Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium

Autores
Pescaretti, María de Las Mercedes; Morero, Roberto Dionisio; Delgado, Monica Alejandra
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis,flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypothesized to act as an intermediary in the phosphotransfer from RcsC to RcsB. The rcsC gene is convergently transcribed toward rcsB, which follows rcsD in what appears to be a two-gene operon. Here, it is reported that the overproduction of the rcsB gene represses rcsD transcription, but has a weak effect on its own expression. We demonstrated that the differential rcsD and rcsB expression is due to the activity of two promoters to transcribe the rcsB gene: (1) PrcsDB located upstream of rcsD and (2) PrcsB located within the rcsD coding region. In addition,here it was demonstrated that in Salmonella typhimurium, PrcsB is important to activate the rcsB expression during the stationary growth phase.
Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
SALMONELLA
GENE TRANSCRIPTION
RCS SYSTEM
RCSB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47745
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar TyphimuriumPescaretti, María de Las MercedesMorero, Roberto DionisioDelgado, Monica AlejandraSALMONELLAGENE TRANSCRIPTIONRCS SYSTEMRCSBhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis,flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypothesized to act as an intermediary in the phosphotransfer from RcsC to RcsB. The rcsC gene is convergently transcribed toward rcsB, which follows rcsD in what appears to be a two-gene operon. Here, it is reported that the overproduction of the rcsB gene represses rcsD transcription, but has a weak effect on its own expression. We demonstrated that the differential rcsD and rcsB expression is due to the activity of two promoters to transcribe the rcsB gene: (1) PrcsDB located upstream of rcsD and (2) PrcsB located within the rcsD coding region. In addition,here it was demonstrated that in Salmonella typhimurium, PrcsB is important to activate the rcsB expression during the stationary growth phase.Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaWiley Blackwell Publishing, Inc2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47745Pescaretti, María de Las Mercedes; Morero, Roberto Dionisio; Delgado, Monica Alejandra; Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 300; 2; 11-2009; 165-1730378-10971574-6968CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/300/2/165/451763info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2009.01771.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:54Zoai:ri.conicet.gov.ar:11336/47745instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:54.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium
title Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium
spellingShingle Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium
Pescaretti, María de Las Mercedes
SALMONELLA
GENE TRANSCRIPTION
RCS SYSTEM
RCSB
title_short Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium
title_full Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium
title_fullStr Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium
title_full_unstemmed Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium
title_sort Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium
dc.creator.none.fl_str_mv Pescaretti, María de Las Mercedes
Morero, Roberto Dionisio
Delgado, Monica Alejandra
author Pescaretti, María de Las Mercedes
author_facet Pescaretti, María de Las Mercedes
Morero, Roberto Dionisio
Delgado, Monica Alejandra
author_role author
author2 Morero, Roberto Dionisio
Delgado, Monica Alejandra
author2_role author
author
dc.subject.none.fl_str_mv SALMONELLA
GENE TRANSCRIPTION
RCS SYSTEM
RCSB
topic SALMONELLA
GENE TRANSCRIPTION
RCS SYSTEM
RCSB
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis,flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypothesized to act as an intermediary in the phosphotransfer from RcsC to RcsB. The rcsC gene is convergently transcribed toward rcsB, which follows rcsD in what appears to be a two-gene operon. Here, it is reported that the overproduction of the rcsB gene represses rcsD transcription, but has a weak effect on its own expression. We demonstrated that the differential rcsD and rcsB expression is due to the activity of two promoters to transcribe the rcsB gene: (1) PrcsDB located upstream of rcsD and (2) PrcsB located within the rcsD coding region. In addition,here it was demonstrated that in Salmonella typhimurium, PrcsB is important to activate the rcsB expression during the stationary growth phase.
Fil: Pescaretti, María de Las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis,flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypothesized to act as an intermediary in the phosphotransfer from RcsC to RcsB. The rcsC gene is convergently transcribed toward rcsB, which follows rcsD in what appears to be a two-gene operon. Here, it is reported that the overproduction of the rcsB gene represses rcsD transcription, but has a weak effect on its own expression. We demonstrated that the differential rcsD and rcsB expression is due to the activity of two promoters to transcribe the rcsB gene: (1) PrcsDB located upstream of rcsD and (2) PrcsB located within the rcsD coding region. In addition,here it was demonstrated that in Salmonella typhimurium, PrcsB is important to activate the rcsB expression during the stationary growth phase.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47745
Pescaretti, María de Las Mercedes; Morero, Roberto Dionisio; Delgado, Monica Alejandra; Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 300; 2; 11-2009; 165-173
0378-1097
1574-6968
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47745
identifier_str_mv Pescaretti, María de Las Mercedes; Morero, Roberto Dionisio; Delgado, Monica Alejandra; Identification of a new promoter for the response regulator rcsB expression in Salmonella enterica serovar Typhimurium; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 300; 2; 11-2009; 165-173
0378-1097
1574-6968
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/300/2/165/451763
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2009.01771.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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