NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity

Autores
Gupta, D. K.; Pena, Liliana Beatriz; Romero Puertas, M. C.; Hernández, A.; Inouhe, M.; Sandalio, L. M.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohFwhich were grown under hydroponic conditions with 25 and 100 μM Cd for 1 and 5 days. Cadmium reduced the growth of leaves in WT,AtrbohC and D, but not in AtrbohF. A time-dependent increase in H2O2 and lipid peroxidation was observed in all genotypes, with AtrbohCshowing the smallest increase. An opposite behaviour was observed with NO accumulation. Cadmium increased catalase activity in WT plants and decreased it in Atrboh mutants, while glutathione reductase and glycolate oxidase activities increased in Atrboh mutants, and superoxide dismutases were down-regulated inAtrbohC. The GSH/GSSG and ASA/DHA couples were also affected by the treatment, principally inAtrbohC and AtrbohF, respectively. Cadmium translocation to the leaves was severely reduced in Atrboh mutants after 1 d of treatment and even after 5 d in AtrbohF. Similar results were observed for S, P, Ca, Zn and Fe accumulation, while an opposite trend was observed for K accumulation, except in AtrbohF. Thus, under Cd stress, RBOHs differentially regulate ROS metabolism, redox homeostasis, and nutrient balance and could be of potential interest in biotechnology for the phytoremediation of polluted soils.
Fil: Gupta, D. K.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España. Leibniz Universität Hannover; Alemania
Fil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Romero Puertas, M. C.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Hernández, A.. Universidad Centroccidental Lisandro Alvarado; Venezuela
Fil: Inouhe, M.. Ehime University Matsuyama; Japón
Fil: Sandalio, L. M.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Materia
Antioxidants
Ascorbate
Cadmium
Glutathione
Nadph Oxidase
Nitric Oxide
Nutrient Imbalance
Oxidative Stress
Reactive Oxygen Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47277
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47277
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicityGupta, D. K.Pena, Liliana BeatrizRomero Puertas, M. C.Hernández, A.Inouhe, M.Sandalio, L. M.AntioxidantsAscorbateCadmiumGlutathioneNadph OxidaseNitric OxideNutrient ImbalanceOxidative StressReactive Oxygen Specieshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohFwhich were grown under hydroponic conditions with 25 and 100 μM Cd for 1 and 5 days. Cadmium reduced the growth of leaves in WT,AtrbohC and D, but not in AtrbohF. A time-dependent increase in H2O2 and lipid peroxidation was observed in all genotypes, with AtrbohCshowing the smallest increase. An opposite behaviour was observed with NO accumulation. Cadmium increased catalase activity in WT plants and decreased it in Atrboh mutants, while glutathione reductase and glycolate oxidase activities increased in Atrboh mutants, and superoxide dismutases were down-regulated inAtrbohC. The GSH/GSSG and ASA/DHA couples were also affected by the treatment, principally inAtrbohC and AtrbohF, respectively. Cadmium translocation to the leaves was severely reduced in Atrboh mutants after 1 d of treatment and even after 5 d in AtrbohF. Similar results were observed for S, P, Ca, Zn and Fe accumulation, while an opposite trend was observed for K accumulation, except in AtrbohF. Thus, under Cd stress, RBOHs differentially regulate ROS metabolism, redox homeostasis, and nutrient balance and could be of potential interest in biotechnology for the phytoremediation of polluted soils.Fil: Gupta, D. K.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España. Leibniz Universität Hannover; AlemaniaFil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Romero Puertas, M. C.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Hernández, A.. Universidad Centroccidental Lisandro Alvarado; VenezuelaFil: Inouhe, M.. Ehime University Matsuyama; JapónFil: Sandalio, L. M.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaWiley Blackwell Publishing, Inc2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47277Gupta, D. K.; Pena, Liliana Beatriz; Romero Puertas, M. C.; Hernández, A.; Inouhe, M.; et al.; NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity; Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 40; 4; 4-2017; 509-5260140-7791CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/pce.12711info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12711info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:40Zoai:ri.conicet.gov.ar:11336/47277instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:40.969CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
title NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
spellingShingle NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
Gupta, D. K.
Antioxidants
Ascorbate
Cadmium
Glutathione
Nadph Oxidase
Nitric Oxide
Nutrient Imbalance
Oxidative Stress
Reactive Oxygen Species
title_short NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
title_full NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
title_fullStr NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
title_full_unstemmed NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
title_sort NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity
dc.creator.none.fl_str_mv Gupta, D. K.
Pena, Liliana Beatriz
Romero Puertas, M. C.
Hernández, A.
Inouhe, M.
Sandalio, L. M.
author Gupta, D. K.
author_facet Gupta, D. K.
Pena, Liliana Beatriz
Romero Puertas, M. C.
Hernández, A.
Inouhe, M.
Sandalio, L. M.
author_role author
author2 Pena, Liliana Beatriz
Romero Puertas, M. C.
Hernández, A.
Inouhe, M.
Sandalio, L. M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Antioxidants
Ascorbate
Cadmium
Glutathione
Nadph Oxidase
Nitric Oxide
Nutrient Imbalance
Oxidative Stress
Reactive Oxygen Species
topic Antioxidants
Ascorbate
Cadmium
Glutathione
Nadph Oxidase
Nitric Oxide
Nutrient Imbalance
Oxidative Stress
Reactive Oxygen Species
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohFwhich were grown under hydroponic conditions with 25 and 100 μM Cd for 1 and 5 days. Cadmium reduced the growth of leaves in WT,AtrbohC and D, but not in AtrbohF. A time-dependent increase in H2O2 and lipid peroxidation was observed in all genotypes, with AtrbohCshowing the smallest increase. An opposite behaviour was observed with NO accumulation. Cadmium increased catalase activity in WT plants and decreased it in Atrboh mutants, while glutathione reductase and glycolate oxidase activities increased in Atrboh mutants, and superoxide dismutases were down-regulated inAtrbohC. The GSH/GSSG and ASA/DHA couples were also affected by the treatment, principally inAtrbohC and AtrbohF, respectively. Cadmium translocation to the leaves was severely reduced in Atrboh mutants after 1 d of treatment and even after 5 d in AtrbohF. Similar results were observed for S, P, Ca, Zn and Fe accumulation, while an opposite trend was observed for K accumulation, except in AtrbohF. Thus, under Cd stress, RBOHs differentially regulate ROS metabolism, redox homeostasis, and nutrient balance and could be of potential interest in biotechnology for the phytoremediation of polluted soils.
Fil: Gupta, D. K.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España. Leibniz Universität Hannover; Alemania
Fil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Romero Puertas, M. C.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Hernández, A.. Universidad Centroccidental Lisandro Alvarado; Venezuela
Fil: Inouhe, M.. Ehime University Matsuyama; Japón
Fil: Sandalio, L. M.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
description The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohFwhich were grown under hydroponic conditions with 25 and 100 μM Cd for 1 and 5 days. Cadmium reduced the growth of leaves in WT,AtrbohC and D, but not in AtrbohF. A time-dependent increase in H2O2 and lipid peroxidation was observed in all genotypes, with AtrbohCshowing the smallest increase. An opposite behaviour was observed with NO accumulation. Cadmium increased catalase activity in WT plants and decreased it in Atrboh mutants, while glutathione reductase and glycolate oxidase activities increased in Atrboh mutants, and superoxide dismutases were down-regulated inAtrbohC. The GSH/GSSG and ASA/DHA couples were also affected by the treatment, principally inAtrbohC and AtrbohF, respectively. Cadmium translocation to the leaves was severely reduced in Atrboh mutants after 1 d of treatment and even after 5 d in AtrbohF. Similar results were observed for S, P, Ca, Zn and Fe accumulation, while an opposite trend was observed for K accumulation, except in AtrbohF. Thus, under Cd stress, RBOHs differentially regulate ROS metabolism, redox homeostasis, and nutrient balance and could be of potential interest in biotechnology for the phytoremediation of polluted soils.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47277
Gupta, D. K.; Pena, Liliana Beatriz; Romero Puertas, M. C.; Hernández, A.; Inouhe, M.; et al.; NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity; Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 40; 4; 4-2017; 509-526
0140-7791
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47277
identifier_str_mv Gupta, D. K.; Pena, Liliana Beatriz; Romero Puertas, M. C.; Hernández, A.; Inouhe, M.; et al.; NADPH oxidases differentially regulate ROS metabolism and nutrient uptake under cadmium toxicity; Wiley Blackwell Publishing, Inc; Plant, Cell and Environment; 40; 4; 4-2017; 509-526
0140-7791
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/pce.12711
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12711
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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