Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate

Autores
Fabiani, Camila; Valero, María Victoria; Basualdo, Jessica; Allegrini, Marco; Iocoli, Gastón Alejandro; Villamil, Maria Bonita; Zabaloy, Maria Celina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anaerobic digestate from manure, a byproduct of biogas production, is increasingly used as an organic fertilizer in circular agriculture systems. This study assessed the microbiological impact of maize fertigation with anaerobic digestate, focusing on fecal indicators (Escherichia coli, Salmonella), antibiotic resistance genes (ARGs), and integrons. The trial was conducted in a commercial maize field, where on-site manure-based anaerobic digestate was applied via center-pivot irrigation. Leaf samples were collected two days (2 dai) and four weeks (4 wai) after the last fertigation. E. coli and Salmonella were assessed by culturable methods, while ARGs and integrons were analyzed by qPCR. Results showed that E. coli (3 MPN/g) and Salmonella were detected at 2 dai but were undetectable at 4 wai and in the control condition, suggesting transient contamination. The abundance of tetW was approximately tenfold higher in digestate-treated plants than in the control, while no consistent changes were observed for the other genes. Overall, fertigation with anaerobic digestate appears to pose minimal microbiological impact within the specific conditions of this study, although it may act as a source of specific resistance determinants. Although limited by the use of single treated and control plots, this study offers preliminary insight into microbial and resistance gene dynamics in the phyllosphere, providing a basis for future replicated hypothesis-driven studies.
Fil: Fabiani, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Valero, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Basualdo, Jessica. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Iocoli, Gastón Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Villamil, Maria Bonita. University of Illinois; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Materia
FEEDLOT MANURE
ANAEROBIC DIGESTION
FECAL INDICATORS
ANTIBIOTIC RESISTANCE GENES
TETW
ERMB
BLACTX-M
INTI1
A-INT1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278667
Acceder
id CONICETDig_6dfb88089df8906fb8e8e746acca8037
oai_identifier_str oai:ri.conicet.gov.ar:11336/278667
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic DigestateFabiani, CamilaValero, María VictoriaBasualdo, JessicaAllegrini, MarcoIocoli, Gastón AlejandroVillamil, Maria BonitaZabaloy, Maria CelinaFEEDLOT MANUREANAEROBIC DIGESTIONFECAL INDICATORSANTIBIOTIC RESISTANCE GENESTETWERMBBLACTX-MINTI1A-INT1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Anaerobic digestate from manure, a byproduct of biogas production, is increasingly used as an organic fertilizer in circular agriculture systems. This study assessed the microbiological impact of maize fertigation with anaerobic digestate, focusing on fecal indicators (Escherichia coli, Salmonella), antibiotic resistance genes (ARGs), and integrons. The trial was conducted in a commercial maize field, where on-site manure-based anaerobic digestate was applied via center-pivot irrigation. Leaf samples were collected two days (2 dai) and four weeks (4 wai) after the last fertigation. E. coli and Salmonella were assessed by culturable methods, while ARGs and integrons were analyzed by qPCR. Results showed that E. coli (3 MPN/g) and Salmonella were detected at 2 dai but were undetectable at 4 wai and in the control condition, suggesting transient contamination. The abundance of tetW was approximately tenfold higher in digestate-treated plants than in the control, while no consistent changes were observed for the other genes. Overall, fertigation with anaerobic digestate appears to pose minimal microbiological impact within the specific conditions of this study, although it may act as a source of specific resistance determinants. Although limited by the use of single treated and control plots, this study offers preliminary insight into microbial and resistance gene dynamics in the phyllosphere, providing a basis for future replicated hypothesis-driven studies.Fil: Fabiani, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Valero, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Basualdo, Jessica. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Iocoli, Gastón Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Villamil, Maria Bonita. University of Illinois; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaMDPI2025-10-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278667Fabiani, Camila; Valero, María Victoria; Basualdo, Jessica; Allegrini, Marco; Iocoli, Gastón Alejandro; et al.; Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate; MDPI; Agronomy; 15; 10; 16-10-2025; 1-162073-4395CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/15/10/2398info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy15102398info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:30:44Zoai:ri.conicet.gov.ar:11336/278667instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:30:45.26CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate
title Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate
spellingShingle Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate
Fabiani, Camila
FEEDLOT MANURE
ANAEROBIC DIGESTION
FECAL INDICATORS
ANTIBIOTIC RESISTANCE GENES
TETW
ERMB
BLACTX-M
INTI1
A-INT1
title_short Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate
title_full Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate
title_fullStr Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate
title_full_unstemmed Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate
title_sort Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate
dc.creator.none.fl_str_mv Fabiani, Camila
Valero, María Victoria
Basualdo, Jessica
Allegrini, Marco
Iocoli, Gastón Alejandro
Villamil, Maria Bonita
Zabaloy, Maria Celina
author Fabiani, Camila
author_facet Fabiani, Camila
Valero, María Victoria
Basualdo, Jessica
Allegrini, Marco
Iocoli, Gastón Alejandro
Villamil, Maria Bonita
Zabaloy, Maria Celina
author_role author
author2 Valero, María Victoria
Basualdo, Jessica
Allegrini, Marco
Iocoli, Gastón Alejandro
Villamil, Maria Bonita
Zabaloy, Maria Celina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv FEEDLOT MANURE
ANAEROBIC DIGESTION
FECAL INDICATORS
ANTIBIOTIC RESISTANCE GENES
TETW
ERMB
BLACTX-M
INTI1
A-INT1
topic FEEDLOT MANURE
ANAEROBIC DIGESTION
FECAL INDICATORS
ANTIBIOTIC RESISTANCE GENES
TETW
ERMB
BLACTX-M
INTI1
A-INT1
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Anaerobic digestate from manure, a byproduct of biogas production, is increasingly used as an organic fertilizer in circular agriculture systems. This study assessed the microbiological impact of maize fertigation with anaerobic digestate, focusing on fecal indicators (Escherichia coli, Salmonella), antibiotic resistance genes (ARGs), and integrons. The trial was conducted in a commercial maize field, where on-site manure-based anaerobic digestate was applied via center-pivot irrigation. Leaf samples were collected two days (2 dai) and four weeks (4 wai) after the last fertigation. E. coli and Salmonella were assessed by culturable methods, while ARGs and integrons were analyzed by qPCR. Results showed that E. coli (3 MPN/g) and Salmonella were detected at 2 dai but were undetectable at 4 wai and in the control condition, suggesting transient contamination. The abundance of tetW was approximately tenfold higher in digestate-treated plants than in the control, while no consistent changes were observed for the other genes. Overall, fertigation with anaerobic digestate appears to pose minimal microbiological impact within the specific conditions of this study, although it may act as a source of specific resistance determinants. Although limited by the use of single treated and control plots, this study offers preliminary insight into microbial and resistance gene dynamics in the phyllosphere, providing a basis for future replicated hypothesis-driven studies.
Fil: Fabiani, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Valero, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Basualdo, Jessica. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Iocoli, Gastón Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Villamil, Maria Bonita. University of Illinois; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zabaloy, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
description Anaerobic digestate from manure, a byproduct of biogas production, is increasingly used as an organic fertilizer in circular agriculture systems. This study assessed the microbiological impact of maize fertigation with anaerobic digestate, focusing on fecal indicators (Escherichia coli, Salmonella), antibiotic resistance genes (ARGs), and integrons. The trial was conducted in a commercial maize field, where on-site manure-based anaerobic digestate was applied via center-pivot irrigation. Leaf samples were collected two days (2 dai) and four weeks (4 wai) after the last fertigation. E. coli and Salmonella were assessed by culturable methods, while ARGs and integrons were analyzed by qPCR. Results showed that E. coli (3 MPN/g) and Salmonella were detected at 2 dai but were undetectable at 4 wai and in the control condition, suggesting transient contamination. The abundance of tetW was approximately tenfold higher in digestate-treated plants than in the control, while no consistent changes were observed for the other genes. Overall, fertigation with anaerobic digestate appears to pose minimal microbiological impact within the specific conditions of this study, although it may act as a source of specific resistance determinants. Although limited by the use of single treated and control plots, this study offers preliminary insight into microbial and resistance gene dynamics in the phyllosphere, providing a basis for future replicated hypothesis-driven studies.
publishDate 2025
dc.date.none.fl_str_mv 2025-10-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278667
Fabiani, Camila; Valero, María Victoria; Basualdo, Jessica; Allegrini, Marco; Iocoli, Gastón Alejandro; et al.; Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate; MDPI; Agronomy; 15; 10; 16-10-2025; 1-16
2073-4395
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278667
identifier_str_mv Fabiani, Camila; Valero, María Victoria; Basualdo, Jessica; Allegrini, Marco; Iocoli, Gastón Alejandro; et al.; Exploratory Field Case Study of Microbial and Resistance Genes Dynamics in the Maize Phyllosphere Following Fertigation with Anaerobic Digestate; MDPI; Agronomy; 15; 10; 16-10-2025; 1-16
2073-4395
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/15/10/2398
info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy15102398
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1858306055184842752
score 12.665996

Publicaciones similares