Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets

Autores
Kokjohn, Tyler A.; Van Vickle, Gregory D.; Maarouf, Chera L.; Kalback, Walter M.; Hunter, Jesse M.; Daugs, Ian D.; Luehrs, Dean C.; Lopez, John; Brune, Daniel; Sue, Lucia I.; Beach, Thomas G.; Castaño, Eduardo Miguel; Roher, Alex E.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Amyloid-β (Aβ) peptides are intimately involved in the inflammatory pathology of atherosclerotic vascular disease (AVD) and Alzheimer's disease (AD). Although substantial amounts of these peptides are produced in the periphery, their role and significance to vascular disease outside the brain requires further investigation. Amyloid-β peptides present in the walls of human aorta atherosclerotic lesions as well as activated and non-activated human platelets were isolated using sequential size-exclusion columns and HPLC reverse-phase methods. The Aβ peptide isolates were quantified by ELISA and structurally analyzed using MALDI-TOF mass spectrometry procedures. Our experiments revealed that both aorta and platelets contained Aβ peptides, predominately Aβ40. The source of the Aβ pool in aortic atherosclerosis lesions is probably the activated platelets and/or vascular wall cells expressing APP/PN2. Significant levels of Aβ42 are present in the plasma, suggesting that this reservoir makes a minor contribution to atherosclerotic plaques. Our data reveal that although aortic atherosclerosis and AD cerebrovascular amyloidosis exhibit clearly divergent end-stage manifestations, both vascular diseases share some key pathophysiological promoting elements and pathways. Whether they happen to be deposited in vessels of the central nervous system or atherosclerotic plaques in the periphery, Aβ peptides may promote and perhaps synergize chronic inflammatory processes which culminate in the degeneration, malfunction and ultimate destruction of arterial walls.
Fil: Kokjohn, Tyler A.. Banner Sun Health Research Institute; Estados Unidos. Midwestern University; Estados Unidos
Fil: Van Vickle, Gregory D.. Banner Sun Health Research Institute; Estados Unidos
Fil: Maarouf, Chera L.. Banner Sun Health Research Institute; Estados Unidos
Fil: Kalback, Walter M.. Banner Sun Health Research Institute; Estados Unidos
Fil: Hunter, Jesse M.. Banner Sun Health Research Institute; Estados Unidos
Fil: Daugs, Ian D.. Banner Sun Health Research Institute; Estados Unidos
Fil: Luehrs, Dean C.. Banner Sun Health Research Institute; Estados Unidos
Fil: Lopez, John. Arizona State University; Estados Unidos
Fil: Brune, Daniel. Arizona State University; Estados Unidos
Fil: Sue, Lucia I.. Banner Sun Health Research Institute; Estados Unidos
Fil: Beach, Thomas G.. Banner Sun Health Research Institute; Estados Unidos
Fil: Castaño, Eduardo Miguel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roher, Alex E.. Banner Sun Health Research Institute; Estados Unidos
Materia
Atherosclerosis
Platelet
Amyloid-Beta
Vascular Inflammation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13722
Acceder
id CONICETDig_6df817d98d4401770981903f1cdc5e75
oai_identifier_str oai:ri.conicet.gov.ar:11336/13722
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and plateletsKokjohn, Tyler A.Van Vickle, Gregory D.Maarouf, Chera L.Kalback, Walter M.Hunter, Jesse M.Daugs, Ian D.Luehrs, Dean C.Lopez, JohnBrune, DanielSue, Lucia I.Beach, Thomas G.Castaño, Eduardo MiguelRoher, Alex E.AtherosclerosisPlateletAmyloid-BetaVascular Inflammationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Amyloid-β (Aβ) peptides are intimately involved in the inflammatory pathology of atherosclerotic vascular disease (AVD) and Alzheimer's disease (AD). Although substantial amounts of these peptides are produced in the periphery, their role and significance to vascular disease outside the brain requires further investigation. Amyloid-β peptides present in the walls of human aorta atherosclerotic lesions as well as activated and non-activated human platelets were isolated using sequential size-exclusion columns and HPLC reverse-phase methods. The Aβ peptide isolates were quantified by ELISA and structurally analyzed using MALDI-TOF mass spectrometry procedures. Our experiments revealed that both aorta and platelets contained Aβ peptides, predominately Aβ40. The source of the Aβ pool in aortic atherosclerosis lesions is probably the activated platelets and/or vascular wall cells expressing APP/PN2. Significant levels of Aβ42 are present in the plasma, suggesting that this reservoir makes a minor contribution to atherosclerotic plaques. Our data reveal that although aortic atherosclerosis and AD cerebrovascular amyloidosis exhibit clearly divergent end-stage manifestations, both vascular diseases share some key pathophysiological promoting elements and pathways. Whether they happen to be deposited in vessels of the central nervous system or atherosclerotic plaques in the periphery, Aβ peptides may promote and perhaps synergize chronic inflammatory processes which culminate in the degeneration, malfunction and ultimate destruction of arterial walls.Fil: Kokjohn, Tyler A.. Banner Sun Health Research Institute; Estados Unidos. Midwestern University; Estados UnidosFil: Van Vickle, Gregory D.. Banner Sun Health Research Institute; Estados UnidosFil: Maarouf, Chera L.. Banner Sun Health Research Institute; Estados UnidosFil: Kalback, Walter M.. Banner Sun Health Research Institute; Estados UnidosFil: Hunter, Jesse M.. Banner Sun Health Research Institute; Estados UnidosFil: Daugs, Ian D.. Banner Sun Health Research Institute; Estados UnidosFil: Luehrs, Dean C.. Banner Sun Health Research Institute; Estados UnidosFil: Lopez, John. Arizona State University; Estados UnidosFil: Brune, Daniel. Arizona State University; Estados UnidosFil: Sue, Lucia I.. Banner Sun Health Research Institute; Estados UnidosFil: Beach, Thomas G.. Banner Sun Health Research Institute; Estados UnidosFil: Castaño, Eduardo Miguel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roher, Alex E.. Banner Sun Health Research Institute; Estados UnidosElsevier Science2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13722Kokjohn, Tyler A.; Van Vickle, Gregory D.; Maarouf, Chera L.; Kalback, Walter M.; Hunter, Jesse M.; et al.; Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets; Elsevier Science; Biochimica Et Biophysica Acta - Molecular Basis Of Disease; 1812; 11; -1-2011; 1508-15140925-4439enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbadis.2011.07.004info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925443911001475info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:10Zoai:ri.conicet.gov.ar:11336/13722instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:11.161CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
title Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
spellingShingle Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
Kokjohn, Tyler A.
Atherosclerosis
Platelet
Amyloid-Beta
Vascular Inflammation
title_short Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
title_full Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
title_fullStr Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
title_full_unstemmed Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
title_sort Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
dc.creator.none.fl_str_mv Kokjohn, Tyler A.
Van Vickle, Gregory D.
Maarouf, Chera L.
Kalback, Walter M.
Hunter, Jesse M.
Daugs, Ian D.
Luehrs, Dean C.
Lopez, John
Brune, Daniel
Sue, Lucia I.
Beach, Thomas G.
Castaño, Eduardo Miguel
Roher, Alex E.
author Kokjohn, Tyler A.
author_facet Kokjohn, Tyler A.
Van Vickle, Gregory D.
Maarouf, Chera L.
Kalback, Walter M.
Hunter, Jesse M.
Daugs, Ian D.
Luehrs, Dean C.
Lopez, John
Brune, Daniel
Sue, Lucia I.
Beach, Thomas G.
Castaño, Eduardo Miguel
Roher, Alex E.
author_role author
author2 Van Vickle, Gregory D.
Maarouf, Chera L.
Kalback, Walter M.
Hunter, Jesse M.
Daugs, Ian D.
Luehrs, Dean C.
Lopez, John
Brune, Daniel
Sue, Lucia I.
Beach, Thomas G.
Castaño, Eduardo Miguel
Roher, Alex E.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Atherosclerosis
Platelet
Amyloid-Beta
Vascular Inflammation
topic Atherosclerosis
Platelet
Amyloid-Beta
Vascular Inflammation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Amyloid-β (Aβ) peptides are intimately involved in the inflammatory pathology of atherosclerotic vascular disease (AVD) and Alzheimer's disease (AD). Although substantial amounts of these peptides are produced in the periphery, their role and significance to vascular disease outside the brain requires further investigation. Amyloid-β peptides present in the walls of human aorta atherosclerotic lesions as well as activated and non-activated human platelets were isolated using sequential size-exclusion columns and HPLC reverse-phase methods. The Aβ peptide isolates were quantified by ELISA and structurally analyzed using MALDI-TOF mass spectrometry procedures. Our experiments revealed that both aorta and platelets contained Aβ peptides, predominately Aβ40. The source of the Aβ pool in aortic atherosclerosis lesions is probably the activated platelets and/or vascular wall cells expressing APP/PN2. Significant levels of Aβ42 are present in the plasma, suggesting that this reservoir makes a minor contribution to atherosclerotic plaques. Our data reveal that although aortic atherosclerosis and AD cerebrovascular amyloidosis exhibit clearly divergent end-stage manifestations, both vascular diseases share some key pathophysiological promoting elements and pathways. Whether they happen to be deposited in vessels of the central nervous system or atherosclerotic plaques in the periphery, Aβ peptides may promote and perhaps synergize chronic inflammatory processes which culminate in the degeneration, malfunction and ultimate destruction of arterial walls.
Fil: Kokjohn, Tyler A.. Banner Sun Health Research Institute; Estados Unidos. Midwestern University; Estados Unidos
Fil: Van Vickle, Gregory D.. Banner Sun Health Research Institute; Estados Unidos
Fil: Maarouf, Chera L.. Banner Sun Health Research Institute; Estados Unidos
Fil: Kalback, Walter M.. Banner Sun Health Research Institute; Estados Unidos
Fil: Hunter, Jesse M.. Banner Sun Health Research Institute; Estados Unidos
Fil: Daugs, Ian D.. Banner Sun Health Research Institute; Estados Unidos
Fil: Luehrs, Dean C.. Banner Sun Health Research Institute; Estados Unidos
Fil: Lopez, John. Arizona State University; Estados Unidos
Fil: Brune, Daniel. Arizona State University; Estados Unidos
Fil: Sue, Lucia I.. Banner Sun Health Research Institute; Estados Unidos
Fil: Beach, Thomas G.. Banner Sun Health Research Institute; Estados Unidos
Fil: Castaño, Eduardo Miguel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roher, Alex E.. Banner Sun Health Research Institute; Estados Unidos
description Amyloid-β (Aβ) peptides are intimately involved in the inflammatory pathology of atherosclerotic vascular disease (AVD) and Alzheimer's disease (AD). Although substantial amounts of these peptides are produced in the periphery, their role and significance to vascular disease outside the brain requires further investigation. Amyloid-β peptides present in the walls of human aorta atherosclerotic lesions as well as activated and non-activated human platelets were isolated using sequential size-exclusion columns and HPLC reverse-phase methods. The Aβ peptide isolates were quantified by ELISA and structurally analyzed using MALDI-TOF mass spectrometry procedures. Our experiments revealed that both aorta and platelets contained Aβ peptides, predominately Aβ40. The source of the Aβ pool in aortic atherosclerosis lesions is probably the activated platelets and/or vascular wall cells expressing APP/PN2. Significant levels of Aβ42 are present in the plasma, suggesting that this reservoir makes a minor contribution to atherosclerotic plaques. Our data reveal that although aortic atherosclerosis and AD cerebrovascular amyloidosis exhibit clearly divergent end-stage manifestations, both vascular diseases share some key pathophysiological promoting elements and pathways. Whether they happen to be deposited in vessels of the central nervous system or atherosclerotic plaques in the periphery, Aβ peptides may promote and perhaps synergize chronic inflammatory processes which culminate in the degeneration, malfunction and ultimate destruction of arterial walls.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13722
Kokjohn, Tyler A.; Van Vickle, Gregory D.; Maarouf, Chera L.; Kalback, Walter M.; Hunter, Jesse M.; et al.; Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets; Elsevier Science; Biochimica Et Biophysica Acta - Molecular Basis Of Disease; 1812; 11; -1-2011; 1508-1514
0925-4439
url http://hdl.handle.net/11336/13722
identifier_str_mv Kokjohn, Tyler A.; Van Vickle, Gregory D.; Maarouf, Chera L.; Kalback, Walter M.; Hunter, Jesse M.; et al.; Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets; Elsevier Science; Biochimica Et Biophysica Acta - Molecular Basis Of Disease; 1812; 11; -1-2011; 1508-1514
0925-4439
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbadis.2011.07.004
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925443911001475
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613548147212288
score 13.070432

Publicaciones similares