Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach

Autores
Pereyra Bonnet, Federico Alberto; Gimeno, Maria Laura; Argumedo, Nelson R.; Ielpi, Marcelo; Cardozo, Johana A.; Giménez, Carla Alejandra; Hyon, Sung Ho; Balzaretti, Vilma Teresa; Loresi, Monica Alejandra; Fainstein Day, Patricia; Litwak, Sara Alejandra; Argibay, Pablo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The conversion of differentiated cells into insulin-producing cells is a promising approach for the autologous replacement of pancreatic cells in patients with type 1 diabetes (T1D). At present, cellular reprogramming strategies encompass ethical problems, epigenetic failure or teratoma formation, which has prompted the development of new approaches. Here, we report a novel technique for the conversion of skin fibroblasts from T1D patients into insulin-expressing clusters using only drug-based induction. Our results demonstrate that skin fibroblasts from diabetic patients have pancreatic differentiation capacities and avoid the necessity of using transgenic strategies, stem cell sources or global demethylation steps. These findings open new possibilities for studying diabetes mechanisms, drug screenings and ultimately autologous transgenic-free regenerative medicine therapies in patients with T1D.
Fil: Pereyra Bonnet, Federico Alberto. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gimeno, Maria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argumedo, Nelson R.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Cardozo, Johana A.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Giménez, Carla Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hyon, Sung Ho. Hospital Italiano; Argentina
Fil: Balzaretti, Vilma Teresa. Hospital Italiano; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano; Argentina
Fil: Fainstein Day, Patricia. Hospital Italiano; Argentina
Fil: Litwak, Sara Alejandra. Hospital Italiano; Argentina
Fil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Cell Transplantation
Diabetes
DNA Methylation
Fibroblast
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34741
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/34741
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free ApproachPereyra Bonnet, Federico AlbertoGimeno, Maria LauraArgumedo, Nelson R.Ielpi, MarceloCardozo, Johana A.Giménez, Carla AlejandraHyon, Sung HoBalzaretti, Vilma TeresaLoresi, Monica AlejandraFainstein Day, PatriciaLitwak, Sara AlejandraArgibay, PabloCell TransplantationDiabetesDNA MethylationFibroblasthttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The conversion of differentiated cells into insulin-producing cells is a promising approach for the autologous replacement of pancreatic cells in patients with type 1 diabetes (T1D). At present, cellular reprogramming strategies encompass ethical problems, epigenetic failure or teratoma formation, which has prompted the development of new approaches. Here, we report a novel technique for the conversion of skin fibroblasts from T1D patients into insulin-expressing clusters using only drug-based induction. Our results demonstrate that skin fibroblasts from diabetic patients have pancreatic differentiation capacities and avoid the necessity of using transgenic strategies, stem cell sources or global demethylation steps. These findings open new possibilities for studying diabetes mechanisms, drug screenings and ultimately autologous transgenic-free regenerative medicine therapies in patients with T1D.Fil: Pereyra Bonnet, Federico Alberto. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gimeno, Maria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Argumedo, Nelson R.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Cardozo, Johana A.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Giménez, Carla Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hyon, Sung Ho. Hospital Italiano; ArgentinaFil: Balzaretti, Vilma Teresa. Hospital Italiano; ArgentinaFil: Loresi, Monica Alejandra. Hospital Italiano; ArgentinaFil: Fainstein Day, Patricia. Hospital Italiano; ArgentinaFil: Litwak, Sara Alejandra. Hospital Italiano; ArgentinaFil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPublic Library of Science2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34741Pereyra Bonnet, Federico Alberto; Gimeno, Maria Laura; Argumedo, Nelson R.; Ielpi, Marcelo; Cardozo, Johana A.; et al.; Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach; Public Library of Science; Plos One; 9; 6; 6-2014; 1-11; e1003691932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0100369info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0100369info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070975/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:51Zoai:ri.conicet.gov.ar:11336/34741instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:51.971CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach
title Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach
spellingShingle Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach
Pereyra Bonnet, Federico Alberto
Cell Transplantation
Diabetes
DNA Methylation
Fibroblast
title_short Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach
title_full Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach
title_fullStr Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach
title_full_unstemmed Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach
title_sort Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach
dc.creator.none.fl_str_mv Pereyra Bonnet, Federico Alberto
Gimeno, Maria Laura
Argumedo, Nelson R.
Ielpi, Marcelo
Cardozo, Johana A.
Giménez, Carla Alejandra
Hyon, Sung Ho
Balzaretti, Vilma Teresa
Loresi, Monica Alejandra
Fainstein Day, Patricia
Litwak, Sara Alejandra
Argibay, Pablo
author Pereyra Bonnet, Federico Alberto
author_facet Pereyra Bonnet, Federico Alberto
Gimeno, Maria Laura
Argumedo, Nelson R.
Ielpi, Marcelo
Cardozo, Johana A.
Giménez, Carla Alejandra
Hyon, Sung Ho
Balzaretti, Vilma Teresa
Loresi, Monica Alejandra
Fainstein Day, Patricia
Litwak, Sara Alejandra
Argibay, Pablo
author_role author
author2 Gimeno, Maria Laura
Argumedo, Nelson R.
Ielpi, Marcelo
Cardozo, Johana A.
Giménez, Carla Alejandra
Hyon, Sung Ho
Balzaretti, Vilma Teresa
Loresi, Monica Alejandra
Fainstein Day, Patricia
Litwak, Sara Alejandra
Argibay, Pablo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell Transplantation
Diabetes
DNA Methylation
Fibroblast
topic Cell Transplantation
Diabetes
DNA Methylation
Fibroblast
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The conversion of differentiated cells into insulin-producing cells is a promising approach for the autologous replacement of pancreatic cells in patients with type 1 diabetes (T1D). At present, cellular reprogramming strategies encompass ethical problems, epigenetic failure or teratoma formation, which has prompted the development of new approaches. Here, we report a novel technique for the conversion of skin fibroblasts from T1D patients into insulin-expressing clusters using only drug-based induction. Our results demonstrate that skin fibroblasts from diabetic patients have pancreatic differentiation capacities and avoid the necessity of using transgenic strategies, stem cell sources or global demethylation steps. These findings open new possibilities for studying diabetes mechanisms, drug screenings and ultimately autologous transgenic-free regenerative medicine therapies in patients with T1D.
Fil: Pereyra Bonnet, Federico Alberto. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gimeno, Maria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argumedo, Nelson R.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Cardozo, Johana A.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Giménez, Carla Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hyon, Sung Ho. Hospital Italiano; Argentina
Fil: Balzaretti, Vilma Teresa. Hospital Italiano; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano; Argentina
Fil: Fainstein Day, Patricia. Hospital Italiano; Argentina
Fil: Litwak, Sara Alejandra. Hospital Italiano; Argentina
Fil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The conversion of differentiated cells into insulin-producing cells is a promising approach for the autologous replacement of pancreatic cells in patients with type 1 diabetes (T1D). At present, cellular reprogramming strategies encompass ethical problems, epigenetic failure or teratoma formation, which has prompted the development of new approaches. Here, we report a novel technique for the conversion of skin fibroblasts from T1D patients into insulin-expressing clusters using only drug-based induction. Our results demonstrate that skin fibroblasts from diabetic patients have pancreatic differentiation capacities and avoid the necessity of using transgenic strategies, stem cell sources or global demethylation steps. These findings open new possibilities for studying diabetes mechanisms, drug screenings and ultimately autologous transgenic-free regenerative medicine therapies in patients with T1D.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34741
Pereyra Bonnet, Federico Alberto; Gimeno, Maria Laura; Argumedo, Nelson R.; Ielpi, Marcelo; Cardozo, Johana A.; et al.; Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach; Public Library of Science; Plos One; 9; 6; 6-2014; 1-11; e100369
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34741
identifier_str_mv Pereyra Bonnet, Federico Alberto; Gimeno, Maria Laura; Argumedo, Nelson R.; Ielpi, Marcelo; Cardozo, Johana A.; et al.; Skin Fibroblasts from Patients with Type 1 Diabetes (T1D) Can Be Chemically Transdifferentiated into Insulin-Expressing Clusters: A Transgene-Free Approach; Public Library of Science; Plos One; 9; 6; 6-2014; 1-11; e100369
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0100369
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0100369
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070975/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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