Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes

Autores
Toneatto, Judith; Lorenzatti, Guadalupe; Cabanillas, Ana Maria de Los A.; Arguello, Gerardo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to investigate the ability of chromium(III) tris-diimine complexes, [Cr(NN)3]3+, to induce DNA photodamage and to examine its capability to impair the survival of irradiated bacteria with the purpose of using these compounds as photocleavage reagents. [Cr(NN)3]3+ complexes, where NN stands for the ligands: phen (1,10-phenanthroline), 5-Mephen, 4,7-diMephen, 3,4,7,8-tetraMephen, 5-Phphen and 5-Clphen were used for this purpose. Their properties of DNA photocleavage were investigated by electrophoretic studies and assays of the photosensitization of transformed bacteria. The results clearly indicate that [Cr(NN)3]3+ complexes promote the photocleavage of plasmid DNA with varied degrees of efficiency after 12 h of irradiation. The combination of DNA, [Cr(NN)3]3+ and light proved to be required to induce the breakdown of DNA. Irradiated plasmid DNA-[Cr(NN) 3]3+ association is also capable of impairing the transforming capacity of bacteria. These results provide evidence which confirms the responsibility and essential role of the excited state of [Cr(NN) 3]3+ for inducing damage. Moreover, assays of the photo sensitization of transformed bacterial suspensions suggest that Escherichia coli may be photo inactivated by irradiation in the presence of [Cr(NN) 3]3+ complexes.
Fil: Toneatto, Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Lorenzatti, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cabanillas, Ana Maria de Los A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Arguello, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Bacterial Growth Photoinhibition
Bacterial Transformation
Chromium(Iii) Complexes
Dna Photocleavage
Photosensitization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54797
Acceder
id CONICETDig_6c73c71423b9cecdfff9684ae5a91738
oai_identifier_str oai:ri.conicet.gov.ar:11336/54797
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel DNA photocleavage properties of [Cr(NN)3]3+ complexesToneatto, JudithLorenzatti, GuadalupeCabanillas, Ana Maria de Los A.Arguello, GerardoBacterial Growth PhotoinhibitionBacterial TransformationChromium(Iii) ComplexesDna PhotocleavagePhotosensitizationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The aim of this study was to investigate the ability of chromium(III) tris-diimine complexes, [Cr(NN)3]3+, to induce DNA photodamage and to examine its capability to impair the survival of irradiated bacteria with the purpose of using these compounds as photocleavage reagents. [Cr(NN)3]3+ complexes, where NN stands for the ligands: phen (1,10-phenanthroline), 5-Mephen, 4,7-diMephen, 3,4,7,8-tetraMephen, 5-Phphen and 5-Clphen were used for this purpose. Their properties of DNA photocleavage were investigated by electrophoretic studies and assays of the photosensitization of transformed bacteria. The results clearly indicate that [Cr(NN)3]3+ complexes promote the photocleavage of plasmid DNA with varied degrees of efficiency after 12 h of irradiation. The combination of DNA, [Cr(NN)3]3+ and light proved to be required to induce the breakdown of DNA. Irradiated plasmid DNA-[Cr(NN) 3]3+ association is also capable of impairing the transforming capacity of bacteria. These results provide evidence which confirms the responsibility and essential role of the excited state of [Cr(NN) 3]3+ for inducing damage. Moreover, assays of the photo sensitization of transformed bacterial suspensions suggest that Escherichia coli may be photo inactivated by irradiation in the presence of [Cr(NN) 3]3+ complexes.Fil: Toneatto, Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Lorenzatti, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Cabanillas, Ana Maria de Los A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Arguello, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54797Toneatto, Judith; Lorenzatti, Guadalupe; Cabanillas, Ana Maria de Los A.; Arguello, Gerardo; Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes; Elsevier Science; Inorganic Chemistry Communications; 15; 1-2012; 43-461387-7003CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1387700311004771info:eu-repo/semantics/altIdentifier/doi/10.1016/j.inoche.2011.09.035info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-17T15:41:28Zoai:ri.conicet.gov.ar:11336/54797instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-17 15:41:28.651CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes
title Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes
spellingShingle Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes
Toneatto, Judith
Bacterial Growth Photoinhibition
Bacterial Transformation
Chromium(Iii) Complexes
Dna Photocleavage
Photosensitization
title_short Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes
title_full Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes
title_fullStr Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes
title_full_unstemmed Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes
title_sort Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes
dc.creator.none.fl_str_mv Toneatto, Judith
Lorenzatti, Guadalupe
Cabanillas, Ana Maria de Los A.
Arguello, Gerardo
author Toneatto, Judith
author_facet Toneatto, Judith
Lorenzatti, Guadalupe
Cabanillas, Ana Maria de Los A.
Arguello, Gerardo
author_role author
author2 Lorenzatti, Guadalupe
Cabanillas, Ana Maria de Los A.
Arguello, Gerardo
author2_role author
author
author
dc.subject.none.fl_str_mv Bacterial Growth Photoinhibition
Bacterial Transformation
Chromium(Iii) Complexes
Dna Photocleavage
Photosensitization
topic Bacterial Growth Photoinhibition
Bacterial Transformation
Chromium(Iii) Complexes
Dna Photocleavage
Photosensitization
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to investigate the ability of chromium(III) tris-diimine complexes, [Cr(NN)3]3+, to induce DNA photodamage and to examine its capability to impair the survival of irradiated bacteria with the purpose of using these compounds as photocleavage reagents. [Cr(NN)3]3+ complexes, where NN stands for the ligands: phen (1,10-phenanthroline), 5-Mephen, 4,7-diMephen, 3,4,7,8-tetraMephen, 5-Phphen and 5-Clphen were used for this purpose. Their properties of DNA photocleavage were investigated by electrophoretic studies and assays of the photosensitization of transformed bacteria. The results clearly indicate that [Cr(NN)3]3+ complexes promote the photocleavage of plasmid DNA with varied degrees of efficiency after 12 h of irradiation. The combination of DNA, [Cr(NN)3]3+ and light proved to be required to induce the breakdown of DNA. Irradiated plasmid DNA-[Cr(NN) 3]3+ association is also capable of impairing the transforming capacity of bacteria. These results provide evidence which confirms the responsibility and essential role of the excited state of [Cr(NN) 3]3+ for inducing damage. Moreover, assays of the photo sensitization of transformed bacterial suspensions suggest that Escherichia coli may be photo inactivated by irradiation in the presence of [Cr(NN) 3]3+ complexes.
Fil: Toneatto, Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Lorenzatti, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cabanillas, Ana Maria de Los A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Arguello, Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description The aim of this study was to investigate the ability of chromium(III) tris-diimine complexes, [Cr(NN)3]3+, to induce DNA photodamage and to examine its capability to impair the survival of irradiated bacteria with the purpose of using these compounds as photocleavage reagents. [Cr(NN)3]3+ complexes, where NN stands for the ligands: phen (1,10-phenanthroline), 5-Mephen, 4,7-diMephen, 3,4,7,8-tetraMephen, 5-Phphen and 5-Clphen were used for this purpose. Their properties of DNA photocleavage were investigated by electrophoretic studies and assays of the photosensitization of transformed bacteria. The results clearly indicate that [Cr(NN)3]3+ complexes promote the photocleavage of plasmid DNA with varied degrees of efficiency after 12 h of irradiation. The combination of DNA, [Cr(NN)3]3+ and light proved to be required to induce the breakdown of DNA. Irradiated plasmid DNA-[Cr(NN) 3]3+ association is also capable of impairing the transforming capacity of bacteria. These results provide evidence which confirms the responsibility and essential role of the excited state of [Cr(NN) 3]3+ for inducing damage. Moreover, assays of the photo sensitization of transformed bacterial suspensions suggest that Escherichia coli may be photo inactivated by irradiation in the presence of [Cr(NN) 3]3+ complexes.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54797
Toneatto, Judith; Lorenzatti, Guadalupe; Cabanillas, Ana Maria de Los A.; Arguello, Gerardo; Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes; Elsevier Science; Inorganic Chemistry Communications; 15; 1-2012; 43-46
1387-7003
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54797
identifier_str_mv Toneatto, Judith; Lorenzatti, Guadalupe; Cabanillas, Ana Maria de Los A.; Arguello, Gerardo; Novel DNA photocleavage properties of [Cr(NN)3]3+ complexes; Elsevier Science; Inorganic Chemistry Communications; 15; 1-2012; 43-46
1387-7003
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1387700311004771
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.inoche.2011.09.035
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176297

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