Antibacterial activity of plant extracts from northwestern Argentina

Autores
Soberon, Jose Rodolfo; Sgariglia, Melina Araceli; Sampietro, Diego Alejandro; Quiroga, Emma Nelly; Vattuone, Marta Amelia
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To determine the antibacterial and cytotoxic activities of aqueous and ethanolic extracts of northwestern Argentinian plants used in folk medicine. To compare the mentioned activities with those of five commercial antibiotics. To identify the compounds responsible for the antibacterial activity. Methods and Results: Plant extracts were prepared according to traditional uses in northwestern Argentina. Antibacterial activity was assayed by agar dilution in Petri dishes and broth dilution in 96-well plates. Lethal dose 50 (LD50) was determined by the Artemia salina assay. Phytochemical analysis was performed by sample adsorption on silica gel, thin-layer chromatography (TLC), bioautography and UV-visible spectra. The results showed that Tripodanthus acutifolius aqueous extracts have lower minimal inhibitory concentrations (MIC) (502 and 506 ìg of extracted material (EM) per ml for infusion and decoction, respectively) than cefotaxim MIC (640 ìg . ml-1) against Acinetobacter freundii (303). These data were lower than their LD50. Tripodanthus acutifolius tincture showed lower MIC (110 ìg of EM per ml) and minimal bactericidal concentration (MBC) (220 ìg of EM per ml) than cefotaxim (MIC and MBC of 320 ìg . ml-1) for Pseudomonas aeruginosa. This extract also showed a MIC/MBC of 110/220 ìg of EM per ml, lower than  oxacillin (MIC/MBC of 160/220 ìg ml-1) for Staphylococcus aureus (ATCC 25923). The cytotoxicity of all extracts were compared with that of commercial antibiotics. Rutin (3,3´,4´,5,7-pentahydroxyflavone 3-â-rhamnosilglucoside), iso-quercitrin (3,3´,4´,5,7-pentahydroxyflavone 3- â -glucoside) and a terpene would be partially responsible for the antibacterial activity of T. acutifolius infusion. Conclusions: Tripodanthus acutifolius extracts had the ability to inhibit bacterial growth. The antibacterial activity differs with the applied extractive method, and it could be partially attributed to glycoflavonoids. This paper contributes to the knowledge of antibacterial capacity of plants from northwestern Argentina. Significance and Impact of the Study: These antibacterial activities support further studies to discover new chemical structures that can contribute to alleviate or cure some illnesses.
Fil: Soberon, Jose Rodolfo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Sgariglia, Melina Araceli. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Quiroga, Emma Nelly. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Materia
Antibacterial
Infusion
Traditional Uses
Tripodanthus Acutifolius
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84541
Acceder
id CONICETDig_6c2024c2a13048508d8ee889b9e987f1
oai_identifier_str oai:ri.conicet.gov.ar:11336/84541
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial activity of plant extracts from northwestern ArgentinaSoberon, Jose RodolfoSgariglia, Melina AraceliSampietro, Diego AlejandroQuiroga, Emma NellyVattuone, Marta AmeliaAntibacterialInfusionTraditional UsesTripodanthus Acutifoliushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To determine the antibacterial and cytotoxic activities of aqueous and ethanolic extracts of northwestern Argentinian plants used in folk medicine. To compare the mentioned activities with those of five commercial antibiotics. To identify the compounds responsible for the antibacterial activity. Methods and Results: Plant extracts were prepared according to traditional uses in northwestern Argentina. Antibacterial activity was assayed by agar dilution in Petri dishes and broth dilution in 96-well plates. Lethal dose 50 (LD50) was determined by the Artemia salina assay. Phytochemical analysis was performed by sample adsorption on silica gel, thin-layer chromatography (TLC), bioautography and UV-visible spectra. The results showed that Tripodanthus acutifolius aqueous extracts have lower minimal inhibitory concentrations (MIC) (502 and 506 ìg of extracted material (EM) per ml for infusion and decoction, respectively) than cefotaxim MIC (640 ìg . ml-1) against Acinetobacter freundii (303). These data were lower than their LD50. Tripodanthus acutifolius tincture showed lower MIC (110 ìg of EM per ml) and minimal bactericidal concentration (MBC) (220 ìg of EM per ml) than cefotaxim (MIC and MBC of 320 ìg . ml-1) for Pseudomonas aeruginosa. This extract also showed a MIC/MBC of 110/220 ìg of EM per ml, lower than  oxacillin (MIC/MBC of 160/220 ìg ml-1) for Staphylococcus aureus (ATCC 25923). The cytotoxicity of all extracts were compared with that of commercial antibiotics. Rutin (3,3´,4´,5,7-pentahydroxyflavone 3-â-rhamnosilglucoside), iso-quercitrin (3,3´,4´,5,7-pentahydroxyflavone 3- â -glucoside) and a terpene would be partially responsible for the antibacterial activity of T. acutifolius infusion. Conclusions: Tripodanthus acutifolius extracts had the ability to inhibit bacterial growth. The antibacterial activity differs with the applied extractive method, and it could be partially attributed to glycoflavonoids. This paper contributes to the knowledge of antibacterial capacity of plants from northwestern Argentina. Significance and Impact of the Study: These antibacterial activities support further studies to discover new chemical structures that can contribute to alleviate or cure some illnesses.Fil: Soberon, Jose Rodolfo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Sgariglia, Melina Araceli. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Quiroga, Emma Nelly. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; ArgentinaFil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaWiley Blackwell Publishing, Inc2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84541Soberon, Jose Rodolfo; Sgariglia, Melina Araceli; Sampietro, Diego Alejandro; Quiroga, Emma Nelly; Vattuone, Marta Amelia; Antibacterial activity of plant extracts from northwestern Argentina; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 102; 6; 6-2007; 1450-14611364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2006.03229.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2006.03229.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:59Zoai:ri.conicet.gov.ar:11336/84541instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:59.573CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial activity of plant extracts from northwestern Argentina
title Antibacterial activity of plant extracts from northwestern Argentina
spellingShingle Antibacterial activity of plant extracts from northwestern Argentina
Soberon, Jose Rodolfo
Antibacterial
Infusion
Traditional Uses
Tripodanthus Acutifolius
title_short Antibacterial activity of plant extracts from northwestern Argentina
title_full Antibacterial activity of plant extracts from northwestern Argentina
title_fullStr Antibacterial activity of plant extracts from northwestern Argentina
title_full_unstemmed Antibacterial activity of plant extracts from northwestern Argentina
title_sort Antibacterial activity of plant extracts from northwestern Argentina
dc.creator.none.fl_str_mv Soberon, Jose Rodolfo
Sgariglia, Melina Araceli
Sampietro, Diego Alejandro
Quiroga, Emma Nelly
Vattuone, Marta Amelia
author Soberon, Jose Rodolfo
author_facet Soberon, Jose Rodolfo
Sgariglia, Melina Araceli
Sampietro, Diego Alejandro
Quiroga, Emma Nelly
Vattuone, Marta Amelia
author_role author
author2 Sgariglia, Melina Araceli
Sampietro, Diego Alejandro
Quiroga, Emma Nelly
Vattuone, Marta Amelia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antibacterial
Infusion
Traditional Uses
Tripodanthus Acutifolius
topic Antibacterial
Infusion
Traditional Uses
Tripodanthus Acutifolius
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To determine the antibacterial and cytotoxic activities of aqueous and ethanolic extracts of northwestern Argentinian plants used in folk medicine. To compare the mentioned activities with those of five commercial antibiotics. To identify the compounds responsible for the antibacterial activity. Methods and Results: Plant extracts were prepared according to traditional uses in northwestern Argentina. Antibacterial activity was assayed by agar dilution in Petri dishes and broth dilution in 96-well plates. Lethal dose 50 (LD50) was determined by the Artemia salina assay. Phytochemical analysis was performed by sample adsorption on silica gel, thin-layer chromatography (TLC), bioautography and UV-visible spectra. The results showed that Tripodanthus acutifolius aqueous extracts have lower minimal inhibitory concentrations (MIC) (502 and 506 ìg of extracted material (EM) per ml for infusion and decoction, respectively) than cefotaxim MIC (640 ìg . ml-1) against Acinetobacter freundii (303). These data were lower than their LD50. Tripodanthus acutifolius tincture showed lower MIC (110 ìg of EM per ml) and minimal bactericidal concentration (MBC) (220 ìg of EM per ml) than cefotaxim (MIC and MBC of 320 ìg . ml-1) for Pseudomonas aeruginosa. This extract also showed a MIC/MBC of 110/220 ìg of EM per ml, lower than  oxacillin (MIC/MBC of 160/220 ìg ml-1) for Staphylococcus aureus (ATCC 25923). The cytotoxicity of all extracts were compared with that of commercial antibiotics. Rutin (3,3´,4´,5,7-pentahydroxyflavone 3-â-rhamnosilglucoside), iso-quercitrin (3,3´,4´,5,7-pentahydroxyflavone 3- â -glucoside) and a terpene would be partially responsible for the antibacterial activity of T. acutifolius infusion. Conclusions: Tripodanthus acutifolius extracts had the ability to inhibit bacterial growth. The antibacterial activity differs with the applied extractive method, and it could be partially attributed to glycoflavonoids. This paper contributes to the knowledge of antibacterial capacity of plants from northwestern Argentina. Significance and Impact of the Study: These antibacterial activities support further studies to discover new chemical structures that can contribute to alleviate or cure some illnesses.
Fil: Soberon, Jose Rodolfo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Sgariglia, Melina Araceli. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Quiroga, Emma Nelly. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
description To determine the antibacterial and cytotoxic activities of aqueous and ethanolic extracts of northwestern Argentinian plants used in folk medicine. To compare the mentioned activities with those of five commercial antibiotics. To identify the compounds responsible for the antibacterial activity. Methods and Results: Plant extracts were prepared according to traditional uses in northwestern Argentina. Antibacterial activity was assayed by agar dilution in Petri dishes and broth dilution in 96-well plates. Lethal dose 50 (LD50) was determined by the Artemia salina assay. Phytochemical analysis was performed by sample adsorption on silica gel, thin-layer chromatography (TLC), bioautography and UV-visible spectra. The results showed that Tripodanthus acutifolius aqueous extracts have lower minimal inhibitory concentrations (MIC) (502 and 506 ìg of extracted material (EM) per ml for infusion and decoction, respectively) than cefotaxim MIC (640 ìg . ml-1) against Acinetobacter freundii (303). These data were lower than their LD50. Tripodanthus acutifolius tincture showed lower MIC (110 ìg of EM per ml) and minimal bactericidal concentration (MBC) (220 ìg of EM per ml) than cefotaxim (MIC and MBC of 320 ìg . ml-1) for Pseudomonas aeruginosa. This extract also showed a MIC/MBC of 110/220 ìg of EM per ml, lower than  oxacillin (MIC/MBC of 160/220 ìg ml-1) for Staphylococcus aureus (ATCC 25923). The cytotoxicity of all extracts were compared with that of commercial antibiotics. Rutin (3,3´,4´,5,7-pentahydroxyflavone 3-â-rhamnosilglucoside), iso-quercitrin (3,3´,4´,5,7-pentahydroxyflavone 3- â -glucoside) and a terpene would be partially responsible for the antibacterial activity of T. acutifolius infusion. Conclusions: Tripodanthus acutifolius extracts had the ability to inhibit bacterial growth. The antibacterial activity differs with the applied extractive method, and it could be partially attributed to glycoflavonoids. This paper contributes to the knowledge of antibacterial capacity of plants from northwestern Argentina. Significance and Impact of the Study: These antibacterial activities support further studies to discover new chemical structures that can contribute to alleviate or cure some illnesses.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84541
Soberon, Jose Rodolfo; Sgariglia, Melina Araceli; Sampietro, Diego Alejandro; Quiroga, Emma Nelly; Vattuone, Marta Amelia; Antibacterial activity of plant extracts from northwestern Argentina; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 102; 6; 6-2007; 1450-1461
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84541
identifier_str_mv Soberon, Jose Rodolfo; Sgariglia, Melina Araceli; Sampietro, Diego Alejandro; Quiroga, Emma Nelly; Vattuone, Marta Amelia; Antibacterial activity of plant extracts from northwestern Argentina; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 102; 6; 6-2007; 1450-1461
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2006.03229.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2006.03229.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614476302647296
score 13.070432

Publicaciones similares