Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media

Autores
Asaro, Antonela; Pinoni, Silvina Andrea; Lorin Nebel, Catherine; Ituarte, Romina Belen
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To understand the response of freshwater organisms to rising environmental salinity, it is essential to investigate their osmo-ionic regulatory physiology. Our laboratory experiment investigated the transfer of Palaemon argentinus Nobili, 1901 from 2‰ (control condition) to concentrated salinity (15, 25 ‰) for short (6 h), medium (48 h) and long-term (> 504 h) acclimation periods. We measured relevant parameters in the shrimp’s haemolymph, the time course of the response of branchial V-H+-ATPase (VHA), Na+, K+-ATPase (NKA), carbonic anhydrase (CA) activity, and muscle water content. Upon prolonged acclimation to 15 ‰ (hyper-regulating condition), shrimp reached a new steady-state of haemolymph osmolality by tightly adjusting ion concentrations to levels higher than the external medium. While NKA and CA activities recovered their pretransfer levels, the downregulation of VHA suggests other functions rather than ion uptake after prolonged acclimation to 15‰. The activity of the three transport-related enzymes remained almost unchanged at the highest salinity (isosmotic condition), leading to increasing osmotic pressure and ion concentration after prolonged acclimation to 25‰. Although the freshwater shrimp studied here retains a certain degree of tolerance to high salinity, a common trait in palaemonid shrimps, our results highlight that 25 ‰ represents a significant hypertonic challenge for this species.
Fil: Asaro, Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Pinoni, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Lorin Nebel, Catherine. Université Montpellier II; Francia
Fil: Ituarte, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
CARBONIC ANHYDRASE
GILLS
IONIC REGULATION
Na+, K+-ATPase
PALAEMON ARGENTINUS
(V)-type H+-ATPase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236519
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/236519
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline mediaAsaro, AntonelaPinoni, Silvina AndreaLorin Nebel, CatherineItuarte, Romina BelenCARBONIC ANHYDRASEGILLSIONIC REGULATIONNa+, K+-ATPasePALAEMON ARGENTINUS(V)-type H+-ATPasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To understand the response of freshwater organisms to rising environmental salinity, it is essential to investigate their osmo-ionic regulatory physiology. Our laboratory experiment investigated the transfer of Palaemon argentinus Nobili, 1901 from 2‰ (control condition) to concentrated salinity (15, 25 ‰) for short (6 h), medium (48 h) and long-term (> 504 h) acclimation periods. We measured relevant parameters in the shrimp’s haemolymph, the time course of the response of branchial V-H+-ATPase (VHA), Na+, K+-ATPase (NKA), carbonic anhydrase (CA) activity, and muscle water content. Upon prolonged acclimation to 15 ‰ (hyper-regulating condition), shrimp reached a new steady-state of haemolymph osmolality by tightly adjusting ion concentrations to levels higher than the external medium. While NKA and CA activities recovered their pretransfer levels, the downregulation of VHA suggests other functions rather than ion uptake after prolonged acclimation to 15‰. The activity of the three transport-related enzymes remained almost unchanged at the highest salinity (isosmotic condition), leading to increasing osmotic pressure and ion concentration after prolonged acclimation to 25‰. Although the freshwater shrimp studied here retains a certain degree of tolerance to high salinity, a common trait in palaemonid shrimps, our results highlight that 25 ‰ represents a significant hypertonic challenge for this species.Fil: Asaro, Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Pinoni, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Lorin Nebel, Catherine. Université Montpellier II; FranciaFil: Ituarte, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaNational Research Council Canada-NRC Research Press2023-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236519Asaro, Antonela; Pinoni, Silvina Andrea; Lorin Nebel, Catherine; Ituarte, Romina Belen; Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 101; 12; 12-2023; 1-390008-4301CONICET DigitalCONICETenghttps://ri.conicet.gov.ar/handle/11336/201004info:eu-repo/semantics/altIdentifier/url/https://cdnsciencepub.com/doi/10.1139/cjz-2023-0056info:eu-repo/semantics/altIdentifier/doi/10.1139/cjz-2023-0056info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:21Zoai:ri.conicet.gov.ar:11336/236519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:21.977CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
title Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
spellingShingle Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
Asaro, Antonela
CARBONIC ANHYDRASE
GILLS
IONIC REGULATION
Na+, K+-ATPase
PALAEMON ARGENTINUS
(V)-type H+-ATPase
title_short Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
title_full Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
title_fullStr Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
title_full_unstemmed Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
title_sort Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media
dc.creator.none.fl_str_mv Asaro, Antonela
Pinoni, Silvina Andrea
Lorin Nebel, Catherine
Ituarte, Romina Belen
author Asaro, Antonela
author_facet Asaro, Antonela
Pinoni, Silvina Andrea
Lorin Nebel, Catherine
Ituarte, Romina Belen
author_role author
author2 Pinoni, Silvina Andrea
Lorin Nebel, Catherine
Ituarte, Romina Belen
author2_role author
author
author
dc.subject.none.fl_str_mv CARBONIC ANHYDRASE
GILLS
IONIC REGULATION
Na+, K+-ATPase
PALAEMON ARGENTINUS
(V)-type H+-ATPase
topic CARBONIC ANHYDRASE
GILLS
IONIC REGULATION
Na+, K+-ATPase
PALAEMON ARGENTINUS
(V)-type H+-ATPase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To understand the response of freshwater organisms to rising environmental salinity, it is essential to investigate their osmo-ionic regulatory physiology. Our laboratory experiment investigated the transfer of Palaemon argentinus Nobili, 1901 from 2‰ (control condition) to concentrated salinity (15, 25 ‰) for short (6 h), medium (48 h) and long-term (> 504 h) acclimation periods. We measured relevant parameters in the shrimp’s haemolymph, the time course of the response of branchial V-H+-ATPase (VHA), Na+, K+-ATPase (NKA), carbonic anhydrase (CA) activity, and muscle water content. Upon prolonged acclimation to 15 ‰ (hyper-regulating condition), shrimp reached a new steady-state of haemolymph osmolality by tightly adjusting ion concentrations to levels higher than the external medium. While NKA and CA activities recovered their pretransfer levels, the downregulation of VHA suggests other functions rather than ion uptake after prolonged acclimation to 15‰. The activity of the three transport-related enzymes remained almost unchanged at the highest salinity (isosmotic condition), leading to increasing osmotic pressure and ion concentration after prolonged acclimation to 25‰. Although the freshwater shrimp studied here retains a certain degree of tolerance to high salinity, a common trait in palaemonid shrimps, our results highlight that 25 ‰ represents a significant hypertonic challenge for this species.
Fil: Asaro, Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Pinoni, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Lorin Nebel, Catherine. Université Montpellier II; Francia
Fil: Ituarte, Romina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description To understand the response of freshwater organisms to rising environmental salinity, it is essential to investigate their osmo-ionic regulatory physiology. Our laboratory experiment investigated the transfer of Palaemon argentinus Nobili, 1901 from 2‰ (control condition) to concentrated salinity (15, 25 ‰) for short (6 h), medium (48 h) and long-term (> 504 h) acclimation periods. We measured relevant parameters in the shrimp’s haemolymph, the time course of the response of branchial V-H+-ATPase (VHA), Na+, K+-ATPase (NKA), carbonic anhydrase (CA) activity, and muscle water content. Upon prolonged acclimation to 15 ‰ (hyper-regulating condition), shrimp reached a new steady-state of haemolymph osmolality by tightly adjusting ion concentrations to levels higher than the external medium. While NKA and CA activities recovered their pretransfer levels, the downregulation of VHA suggests other functions rather than ion uptake after prolonged acclimation to 15‰. The activity of the three transport-related enzymes remained almost unchanged at the highest salinity (isosmotic condition), leading to increasing osmotic pressure and ion concentration after prolonged acclimation to 25‰. Although the freshwater shrimp studied here retains a certain degree of tolerance to high salinity, a common trait in palaemonid shrimps, our results highlight that 25 ‰ represents a significant hypertonic challenge for this species.
publishDate 2023
dc.date.none.fl_str_mv 2023-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236519
Asaro, Antonela; Pinoni, Silvina Andrea; Lorin Nebel, Catherine; Ituarte, Romina Belen; Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 101; 12; 12-2023; 1-39
0008-4301
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236519
identifier_str_mv Asaro, Antonela; Pinoni, Silvina Andrea; Lorin Nebel, Catherine; Ituarte, Romina Belen; Transport-related enzymes and osmo-ionic regulation in a euryhaline freshwater shrimp after transfer to saline media; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 101; 12; 12-2023; 1-39
0008-4301
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ri.conicet.gov.ar/handle/11336/201004
info:eu-repo/semantics/altIdentifier/url/https://cdnsciencepub.com/doi/10.1139/cjz-2023-0056
info:eu-repo/semantics/altIdentifier/doi/10.1139/cjz-2023-0056
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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