Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Spec...

Autores
Aagaard, C.; Govaerts, M.; Meikle, V.; Vallecillo, A. J.; Gutierrez Pabello, J. A.; Suarez Güemes, F.; McNair, J.; Cataldi, Ángel Adrián; Espitia, C.; Andersen, P.; Pollock, J. M.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine tuberculosis is a major problem in many countries; hence, new and better diagnostic tools are urgently needed. In this work, we have tested ESAT6, CFP10, PE13, PE5, MPB70, TB10.4, and TB27.4 for their potentials as diagnostic markers in field animals from Northern Ireland, Mexico, and Argentina, regions with low, medium, and high prevalences of bovine tuberculosis, respectively. At all three sites, ESAT6 and CFP10 were superior diagnostic antigens, while their combination performed even better at the two sites where the combination was tested, providing the best coverage for the detection of diseased populations. The high sensitivity in the skin test reactor groups, combined with the high specificity in the tuberculosis-free groups, indicated that a diagnosis could correctly be made for 85% of the infected animals, based on their responses to these two antigens. Furthermore, TB10.4, PE13, and PE5 have the potential to supplement ESAT6 and CFP10 in a future five-component diagnostic cocktail.
Fil: Aagaard, C.. No especifíca;
Fil: Govaerts, M.. Queens University; Canadá
Fil: Meikle, V.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vallecillo, A. J.. Universidad Nacional Autónoma de México; México
Fil: Gutierrez Pabello, J. A.. Universidad Nacional Autónoma de México; México
Fil: Suarez Güemes, F.. Universidad Nacional Autónoma de México; México
Fil: McNair, J.. No especifíca;
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Espitia, C.. Universidad Nacional Autónoma de México; México
Fil: Andersen, P.. No especifíca;
Fil: Pollock, J. M.. No especifíca;
Materia
antigen
Bovine tuberculosis
Diagnosis
IFNg
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244787
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/244787
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and SpecificityAagaard, C.Govaerts, M.Meikle, V.Vallecillo, A. J.Gutierrez Pabello, J. A.Suarez Güemes, F.McNair, J.Cataldi, Ángel AdriánEspitia, C.Andersen, P.Pollock, J. M.antigenBovine tuberculosisDiagnosisIFNghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bovine tuberculosis is a major problem in many countries; hence, new and better diagnostic tools are urgently needed. In this work, we have tested ESAT6, CFP10, PE13, PE5, MPB70, TB10.4, and TB27.4 for their potentials as diagnostic markers in field animals from Northern Ireland, Mexico, and Argentina, regions with low, medium, and high prevalences of bovine tuberculosis, respectively. At all three sites, ESAT6 and CFP10 were superior diagnostic antigens, while their combination performed even better at the two sites where the combination was tested, providing the best coverage for the detection of diseased populations. The high sensitivity in the skin test reactor groups, combined with the high specificity in the tuberculosis-free groups, indicated that a diagnosis could correctly be made for 85% of the infected animals, based on their responses to these two antigens. Furthermore, TB10.4, PE13, and PE5 have the potential to supplement ESAT6 and CFP10 in a future five-component diagnostic cocktail.Fil: Aagaard, C.. No especifíca;Fil: Govaerts, M.. Queens University; CanadáFil: Meikle, V.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Vallecillo, A. J.. Universidad Nacional Autónoma de México; MéxicoFil: Gutierrez Pabello, J. A.. Universidad Nacional Autónoma de México; MéxicoFil: Suarez Güemes, F.. Universidad Nacional Autónoma de México; MéxicoFil: McNair, J.. No especifíca;Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Espitia, C.. Universidad Nacional Autónoma de México; MéxicoFil: Andersen, P.. No especifíca;Fil: Pollock, J. M.. No especifíca;American Society for Microbiology2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244787Aagaard, C.; Govaerts, M.; Meikle, V.; Vallecillo, A. J.; Gutierrez Pabello, J. A.; et al.; Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity; American Society for Microbiology; Journal of Clinical Microbiology; 44; 12; 12-2006; 4326-43350095-1137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/jcm.01184-06info:eu-repo/semantics/altIdentifier/doi/10.1128/jcm.01184-06info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:48:06Zoai:ri.conicet.gov.ar:11336/244787instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:48:07.277CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity
title Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity
spellingShingle Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity
Aagaard, C.
antigen
Bovine tuberculosis
Diagnosis
IFNg
title_short Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity
title_full Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity
title_fullStr Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity
title_full_unstemmed Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity
title_sort Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity
dc.creator.none.fl_str_mv Aagaard, C.
Govaerts, M.
Meikle, V.
Vallecillo, A. J.
Gutierrez Pabello, J. A.
Suarez Güemes, F.
McNair, J.
Cataldi, Ángel Adrián
Espitia, C.
Andersen, P.
Pollock, J. M.
author Aagaard, C.
author_facet Aagaard, C.
Govaerts, M.
Meikle, V.
Vallecillo, A. J.
Gutierrez Pabello, J. A.
Suarez Güemes, F.
McNair, J.
Cataldi, Ángel Adrián
Espitia, C.
Andersen, P.
Pollock, J. M.
author_role author
author2 Govaerts, M.
Meikle, V.
Vallecillo, A. J.
Gutierrez Pabello, J. A.
Suarez Güemes, F.
McNair, J.
Cataldi, Ángel Adrián
Espitia, C.
Andersen, P.
Pollock, J. M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv antigen
Bovine tuberculosis
Diagnosis
IFNg
topic antigen
Bovine tuberculosis
Diagnosis
IFNg
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bovine tuberculosis is a major problem in many countries; hence, new and better diagnostic tools are urgently needed. In this work, we have tested ESAT6, CFP10, PE13, PE5, MPB70, TB10.4, and TB27.4 for their potentials as diagnostic markers in field animals from Northern Ireland, Mexico, and Argentina, regions with low, medium, and high prevalences of bovine tuberculosis, respectively. At all three sites, ESAT6 and CFP10 were superior diagnostic antigens, while their combination performed even better at the two sites where the combination was tested, providing the best coverage for the detection of diseased populations. The high sensitivity in the skin test reactor groups, combined with the high specificity in the tuberculosis-free groups, indicated that a diagnosis could correctly be made for 85% of the infected animals, based on their responses to these two antigens. Furthermore, TB10.4, PE13, and PE5 have the potential to supplement ESAT6 and CFP10 in a future five-component diagnostic cocktail.
Fil: Aagaard, C.. No especifíca;
Fil: Govaerts, M.. Queens University; Canadá
Fil: Meikle, V.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vallecillo, A. J.. Universidad Nacional Autónoma de México; México
Fil: Gutierrez Pabello, J. A.. Universidad Nacional Autónoma de México; México
Fil: Suarez Güemes, F.. Universidad Nacional Autónoma de México; México
Fil: McNair, J.. No especifíca;
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Espitia, C.. Universidad Nacional Autónoma de México; México
Fil: Andersen, P.. No especifíca;
Fil: Pollock, J. M.. No especifíca;
description Bovine tuberculosis is a major problem in many countries; hence, new and better diagnostic tools are urgently needed. In this work, we have tested ESAT6, CFP10, PE13, PE5, MPB70, TB10.4, and TB27.4 for their potentials as diagnostic markers in field animals from Northern Ireland, Mexico, and Argentina, regions with low, medium, and high prevalences of bovine tuberculosis, respectively. At all three sites, ESAT6 and CFP10 were superior diagnostic antigens, while their combination performed even better at the two sites where the combination was tested, providing the best coverage for the detection of diseased populations. The high sensitivity in the skin test reactor groups, combined with the high specificity in the tuberculosis-free groups, indicated that a diagnosis could correctly be made for 85% of the infected animals, based on their responses to these two antigens. Furthermore, TB10.4, PE13, and PE5 have the potential to supplement ESAT6 and CFP10 in a future five-component diagnostic cocktail.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244787
Aagaard, C.; Govaerts, M.; Meikle, V.; Vallecillo, A. J.; Gutierrez Pabello, J. A.; et al.; Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity; American Society for Microbiology; Journal of Clinical Microbiology; 44; 12; 12-2006; 4326-4335
0095-1137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244787
identifier_str_mv Aagaard, C.; Govaerts, M.; Meikle, V.; Vallecillo, A. J.; Gutierrez Pabello, J. A.; et al.; Optimizing Antigen Cocktails for Detection of Mycobacterium bovis in Herds with Different Prevalences of Bovine Tuberculosis: ESAT6-CFP10 Mixture Shows Optimal Sensitivity and Specificity; American Society for Microbiology; Journal of Clinical Microbiology; 44; 12; 12-2006; 4326-4335
0095-1137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/jcm.01184-06
info:eu-repo/semantics/altIdentifier/doi/10.1128/jcm.01184-06
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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