Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish

Autores
Rojas, Diego A.; Perez Munizaga, Daniela A.; Centanin, Lazaro; Antonelli, Marcelo; Wappner, Pablo; Allende, Miguel L.; Reyes, Ariel E.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1alpha and hif-2alpha genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1alpha and hif-2alpha genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1alpha and hif-2alpha are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1alpha is switched off, while hif-2alpha transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1alpha and hif-2alpha is directly assessed in a vertebrate model system throughout development
Fil: Rojas, Diego A.. Universidad de Chile; Chile
Fil: Perez Munizaga, Daniela A.. Universidad de Chile; Chile
Fil: Centanin, Lazaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Antonelli, Marcelo. Universidad de Chile; Chile
Fil: Wappner, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Allende, Miguel L.. Universidad de Chile; Chile
Fil: Reyes, Ariel E.. Universidad de Chile; Chile
Materia
Hif
Zebra Fish
Development
Circulatory System
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28384
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/28384
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafishRojas, Diego A.Perez Munizaga, Daniela A.Centanin, LazaroAntonelli, MarceloWappner, PabloAllende, Miguel L.Reyes, Ariel E.HifZebra FishDevelopmentCirculatory Systemhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1alpha and hif-2alpha genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1alpha and hif-2alpha genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1alpha and hif-2alpha are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1alpha is switched off, while hif-2alpha transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1alpha and hif-2alpha is directly assessed in a vertebrate model system throughout developmentFil: Rojas, Diego A.. Universidad de Chile; ChileFil: Perez Munizaga, Daniela A.. Universidad de Chile; ChileFil: Centanin, Lazaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Antonelli, Marcelo. Universidad de Chile; ChileFil: Wappner, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Allende, Miguel L.. Universidad de Chile; ChileFil: Reyes, Ariel E.. Universidad de Chile; ChileElsevier2007-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28384Rojas, Diego A.; Perez Munizaga, Daniela A.; Centanin, Lazaro; Antonelli, Marcelo; Wappner, Pablo; et al.; Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish; Elsevier; Gene Expression Patterns; 7; 3; 1-2007; 339-3451567-133X1872-7298CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567133X06001372info:eu-repo/semantics/altIdentifier/doi/10.1016/j.modgep.2006.08.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:23:58Zoai:ri.conicet.gov.ar:11336/28384instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:23:58.512CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish
title Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish
spellingShingle Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish
Rojas, Diego A.
Hif
Zebra Fish
Development
Circulatory System
title_short Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish
title_full Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish
title_fullStr Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish
title_full_unstemmed Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish
title_sort Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish
dc.creator.none.fl_str_mv Rojas, Diego A.
Perez Munizaga, Daniela A.
Centanin, Lazaro
Antonelli, Marcelo
Wappner, Pablo
Allende, Miguel L.
Reyes, Ariel E.
author Rojas, Diego A.
author_facet Rojas, Diego A.
Perez Munizaga, Daniela A.
Centanin, Lazaro
Antonelli, Marcelo
Wappner, Pablo
Allende, Miguel L.
Reyes, Ariel E.
author_role author
author2 Perez Munizaga, Daniela A.
Centanin, Lazaro
Antonelli, Marcelo
Wappner, Pablo
Allende, Miguel L.
Reyes, Ariel E.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hif
Zebra Fish
Development
Circulatory System
topic Hif
Zebra Fish
Development
Circulatory System
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1alpha and hif-2alpha genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1alpha and hif-2alpha genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1alpha and hif-2alpha are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1alpha is switched off, while hif-2alpha transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1alpha and hif-2alpha is directly assessed in a vertebrate model system throughout development
Fil: Rojas, Diego A.. Universidad de Chile; Chile
Fil: Perez Munizaga, Daniela A.. Universidad de Chile; Chile
Fil: Centanin, Lazaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Antonelli, Marcelo. Universidad de Chile; Chile
Fil: Wappner, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Allende, Miguel L.. Universidad de Chile; Chile
Fil: Reyes, Ariel E.. Universidad de Chile; Chile
description Hypoxia-inducible factors (HIFs) regulate gene expression in response to hypoxia and in vertebrates they are known to participate in several developmental processes, including angiogenesis, vasculogenesis, heart and central nervous system development. Over the last decade, major progress in unraveling the molecular mechanisms that mediate regulation of HIF proteins by oxygen tension has been reported, but our knowledge on their developmental regulation during embryogenesis in model organisms is limited. Expression of hif-1alpha and hif-2alpha genes has been characterized during normal mouse development and they were found to be expressed from stages E7.5, later in E9.5 and E15.5 in several different tissues such as the brain, heart and blood vessels. However, there is no detailed temporal information on their expression at other embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe the cloning and detailed expression pattern of zebrafish hif-1alpha and hif-2alpha genes. Sequence analysis revealed that zebrafish Hif proteins are highly homologous to other vertebrate orthologues. Zebrafish hif-1alpha and hif-2alpha are both expressed throughout development in discrete territories in a dynamic pattern. Interestingly, in the notochord the expression of hif-1alpha is switched off, while hif-2alpha transcription is turned on, signifying that the two genes might have partially overlapping, although non-redundant functions in development. This is the first time that a detailed comparison of the expression of hif-1alpha and hif-2alpha is directly assessed in a vertebrate model system throughout development
publishDate 2007
dc.date.none.fl_str_mv 2007-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28384
Rojas, Diego A.; Perez Munizaga, Daniela A.; Centanin, Lazaro; Antonelli, Marcelo; Wappner, Pablo; et al.; Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish; Elsevier; Gene Expression Patterns; 7; 3; 1-2007; 339-345
1567-133X
1872-7298
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28384
identifier_str_mv Rojas, Diego A.; Perez Munizaga, Daniela A.; Centanin, Lazaro; Antonelli, Marcelo; Wappner, Pablo; et al.; Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish; Elsevier; Gene Expression Patterns; 7; 3; 1-2007; 339-345
1567-133X
1872-7298
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567133X06001372
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.modgep.2006.08.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.121305

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