Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions

Autores
Kivlehan, Francine; Garay, Fernando Sebastian; Guo, Jidong; Chaum, Edward; Lindner, Ernö
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Propofol is a widely used, potent intravenous anesthetic for ambulatory anesthesia and long-term sedation. The target steady state concentration of propofol in blood is 0.25-10 μg/mL (1-60 μM). Although propofol can be oxidized electrochemically, monitoring its concentration in biological matrixes is very challenging due to (i) low therapeutic concentration, (ii) high concentrations of easily oxidizable interfering compounds in the sample, and (iii) fouling of the working electrode. In this work we report the performance characteristics of an organic film coated glassy carbon (GC) electrode for continuous monitoring of propofol. The organic film (a plasticized PVC membrane) improved the detection limit and the selectivity of the voltammetric sensor due to the large difference in hydrophobicity between the analyte (propofol) and interfering compounds of the sample, e.g., ascorbic acid (AA) or p-acetamidophenol (APAP). Furthermore, the membrane coating prevented electrode fouling and served as a protective barrier against electrode passivation by proteins. Studies revealed that sensitivity and selectivity of the voltammetric method is greatly influenced by the composition of the PVC membrane. The detection limit of the membrane-coated sensor for propofol in PBS is reported as 0.03 ± 0.01 μM. In serum-like electrolyte solutions containing physiologically relevant levels of albumin (5%) and 3 mM AA and 1 mM APAP as interfering agents, the detection limit was 0.5 ± 0.4 μM. Both values are below the target concentrations used clinically during anesthesia or sedation. © 2012 American Chemical Society.
Fil: Kivlehan, Francine. University of Memphis; Estados Unidos
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Guo, Jidong. University of Memphis; Estados Unidos
Fil: Chaum, Edward. University of Tennessee; Estados Unidos
Fil: Lindner, Ernö. University of Memphis; Estados Unidos
Materia
Propofol
Membrane-Coated Sensor
Passivation
Voltammetry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61960
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutionsKivlehan, FrancineGaray, Fernando SebastianGuo, JidongChaum, EdwardLindner, ErnöPropofolMembrane-Coated SensorPassivationVoltammetryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Propofol is a widely used, potent intravenous anesthetic for ambulatory anesthesia and long-term sedation. The target steady state concentration of propofol in blood is 0.25-10 μg/mL (1-60 μM). Although propofol can be oxidized electrochemically, monitoring its concentration in biological matrixes is very challenging due to (i) low therapeutic concentration, (ii) high concentrations of easily oxidizable interfering compounds in the sample, and (iii) fouling of the working electrode. In this work we report the performance characteristics of an organic film coated glassy carbon (GC) electrode for continuous monitoring of propofol. The organic film (a plasticized PVC membrane) improved the detection limit and the selectivity of the voltammetric sensor due to the large difference in hydrophobicity between the analyte (propofol) and interfering compounds of the sample, e.g., ascorbic acid (AA) or p-acetamidophenol (APAP). Furthermore, the membrane coating prevented electrode fouling and served as a protective barrier against electrode passivation by proteins. Studies revealed that sensitivity and selectivity of the voltammetric method is greatly influenced by the composition of the PVC membrane. The detection limit of the membrane-coated sensor for propofol in PBS is reported as 0.03 ± 0.01 μM. In serum-like electrolyte solutions containing physiologically relevant levels of albumin (5%) and 3 mM AA and 1 mM APAP as interfering agents, the detection limit was 0.5 ± 0.4 μM. Both values are below the target concentrations used clinically during anesthesia or sedation. © 2012 American Chemical Society.Fil: Kivlehan, Francine. University of Memphis; Estados UnidosFil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Guo, Jidong. University of Memphis; Estados UnidosFil: Chaum, Edward. University of Tennessee; Estados UnidosFil: Lindner, Ernö. University of Memphis; Estados UnidosAmerican Chemical Society2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61960Kivlehan, Francine; Garay, Fernando Sebastian; Guo, Jidong; Chaum, Edward; Lindner, Ernö; Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions; American Chemical Society; Analytical Chemistry; 84; 18; 9-2012; 7670-76760003-2700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/ac3006878info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/ac3006878info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:31:09Zoai:ri.conicet.gov.ar:11336/61960instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:31:09.921CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions
title Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions
spellingShingle Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions
Kivlehan, Francine
Propofol
Membrane-Coated Sensor
Passivation
Voltammetry
title_short Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions
title_full Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions
title_fullStr Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions
title_full_unstemmed Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions
title_sort Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions
dc.creator.none.fl_str_mv Kivlehan, Francine
Garay, Fernando Sebastian
Guo, Jidong
Chaum, Edward
Lindner, Ernö
author Kivlehan, Francine
author_facet Kivlehan, Francine
Garay, Fernando Sebastian
Guo, Jidong
Chaum, Edward
Lindner, Ernö
author_role author
author2 Garay, Fernando Sebastian
Guo, Jidong
Chaum, Edward
Lindner, Ernö
author2_role author
author
author
author
dc.subject.none.fl_str_mv Propofol
Membrane-Coated Sensor
Passivation
Voltammetry
topic Propofol
Membrane-Coated Sensor
Passivation
Voltammetry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Propofol is a widely used, potent intravenous anesthetic for ambulatory anesthesia and long-term sedation. The target steady state concentration of propofol in blood is 0.25-10 μg/mL (1-60 μM). Although propofol can be oxidized electrochemically, monitoring its concentration in biological matrixes is very challenging due to (i) low therapeutic concentration, (ii) high concentrations of easily oxidizable interfering compounds in the sample, and (iii) fouling of the working electrode. In this work we report the performance characteristics of an organic film coated glassy carbon (GC) electrode for continuous monitoring of propofol. The organic film (a plasticized PVC membrane) improved the detection limit and the selectivity of the voltammetric sensor due to the large difference in hydrophobicity between the analyte (propofol) and interfering compounds of the sample, e.g., ascorbic acid (AA) or p-acetamidophenol (APAP). Furthermore, the membrane coating prevented electrode fouling and served as a protective barrier against electrode passivation by proteins. Studies revealed that sensitivity and selectivity of the voltammetric method is greatly influenced by the composition of the PVC membrane. The detection limit of the membrane-coated sensor for propofol in PBS is reported as 0.03 ± 0.01 μM. In serum-like electrolyte solutions containing physiologically relevant levels of albumin (5%) and 3 mM AA and 1 mM APAP as interfering agents, the detection limit was 0.5 ± 0.4 μM. Both values are below the target concentrations used clinically during anesthesia or sedation. © 2012 American Chemical Society.
Fil: Kivlehan, Francine. University of Memphis; Estados Unidos
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Guo, Jidong. University of Memphis; Estados Unidos
Fil: Chaum, Edward. University of Tennessee; Estados Unidos
Fil: Lindner, Ernö. University of Memphis; Estados Unidos
description Propofol is a widely used, potent intravenous anesthetic for ambulatory anesthesia and long-term sedation. The target steady state concentration of propofol in blood is 0.25-10 μg/mL (1-60 μM). Although propofol can be oxidized electrochemically, monitoring its concentration in biological matrixes is very challenging due to (i) low therapeutic concentration, (ii) high concentrations of easily oxidizable interfering compounds in the sample, and (iii) fouling of the working electrode. In this work we report the performance characteristics of an organic film coated glassy carbon (GC) electrode for continuous monitoring of propofol. The organic film (a plasticized PVC membrane) improved the detection limit and the selectivity of the voltammetric sensor due to the large difference in hydrophobicity between the analyte (propofol) and interfering compounds of the sample, e.g., ascorbic acid (AA) or p-acetamidophenol (APAP). Furthermore, the membrane coating prevented electrode fouling and served as a protective barrier against electrode passivation by proteins. Studies revealed that sensitivity and selectivity of the voltammetric method is greatly influenced by the composition of the PVC membrane. The detection limit of the membrane-coated sensor for propofol in PBS is reported as 0.03 ± 0.01 μM. In serum-like electrolyte solutions containing physiologically relevant levels of albumin (5%) and 3 mM AA and 1 mM APAP as interfering agents, the detection limit was 0.5 ± 0.4 μM. Both values are below the target concentrations used clinically during anesthesia or sedation. © 2012 American Chemical Society.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61960
Kivlehan, Francine; Garay, Fernando Sebastian; Guo, Jidong; Chaum, Edward; Lindner, Ernö; Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions; American Chemical Society; Analytical Chemistry; 84; 18; 9-2012; 7670-7676
0003-2700
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61960
identifier_str_mv Kivlehan, Francine; Garay, Fernando Sebastian; Guo, Jidong; Chaum, Edward; Lindner, Ernö; Toward feedback-controlled anesthesia: Voltammetric measurement of propofol (2,6-diisopropylphenol) in serum-like electrolyte solutions; American Chemical Society; Analytical Chemistry; 84; 18; 9-2012; 7670-7676
0003-2700
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/ac3006878
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/ac3006878
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.891075

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