Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
- Autores
- Zelenková, Tereza; Onnainty, Renée; Granero, Gladys Ester; Barresi, Antonello A.; Fissore, Davide
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed.
Fil: Zelenková, Tereza. Politecnico di Torino; Italia
Fil: Onnainty, Renée. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Barresi, Antonello A.. Politecnico di Torino; Italia
Fil: Fissore, Davide. Politecnico di Torino; Italia - Materia
-
CHITOSAN
CONFINED IMPINGING JETS MIXER
FREEZE-DRYING
MULTI-INLET VORTEX MIXER
NANOPARTICLES
NANOTECHNOLOGY
SOLVENT DISPLACEMENT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/92597
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/92597 |
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network_name_str |
CONICET Digital (CONICET) |
spelling |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticlesZelenková, TerezaOnnainty, RenéeGranero, Gladys EsterBarresi, Antonello A.Fissore, DavideCHITOSANCONFINED IMPINGING JETS MIXERFREEZE-DRYINGMULTI-INLET VORTEX MIXERNANOPARTICLESNANOTECHNOLOGYSOLVENT DISPLACEMENThttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed.Fil: Zelenková, Tereza. Politecnico di Torino; ItaliaFil: Onnainty, Renée. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Barresi, Antonello A.. Politecnico di Torino; ItaliaFil: Fissore, Davide. Politecnico di Torino; ItaliaElsevier Science2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92597Zelenková, Tereza; Onnainty, Renée; Granero, Gladys Ester; Barresi, Antonello A.; Fissore, Davide; Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles; Elsevier Science; European Journal Of Pharmaceutical Sciences; 119; 7-2018; 135-1460928-0987CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0928098718301611info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejps.2018.04.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:05Zoai:ri.conicet.gov.ar:11336/92597instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:06.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles |
title |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles |
spellingShingle |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles Zelenková, Tereza CHITOSAN CONFINED IMPINGING JETS MIXER FREEZE-DRYING MULTI-INLET VORTEX MIXER NANOPARTICLES NANOTECHNOLOGY SOLVENT DISPLACEMENT |
title_short |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles |
title_full |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles |
title_fullStr |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles |
title_full_unstemmed |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles |
title_sort |
Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles |
dc.creator.none.fl_str_mv |
Zelenková, Tereza Onnainty, Renée Granero, Gladys Ester Barresi, Antonello A. Fissore, Davide |
author |
Zelenková, Tereza |
author_facet |
Zelenková, Tereza Onnainty, Renée Granero, Gladys Ester Barresi, Antonello A. Fissore, Davide |
author_role |
author |
author2 |
Onnainty, Renée Granero, Gladys Ester Barresi, Antonello A. Fissore, Davide |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
CHITOSAN CONFINED IMPINGING JETS MIXER FREEZE-DRYING MULTI-INLET VORTEX MIXER NANOPARTICLES NANOTECHNOLOGY SOLVENT DISPLACEMENT |
topic |
CHITOSAN CONFINED IMPINGING JETS MIXER FREEZE-DRYING MULTI-INLET VORTEX MIXER NANOPARTICLES NANOTECHNOLOGY SOLVENT DISPLACEMENT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed. Fil: Zelenková, Tereza. Politecnico di Torino; Italia Fil: Onnainty, Renée. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Barresi, Antonello A.. Politecnico di Torino; Italia Fil: Fissore, Davide. Politecnico di Torino; Italia |
description |
This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/92597 Zelenková, Tereza; Onnainty, Renée; Granero, Gladys Ester; Barresi, Antonello A.; Fissore, Davide; Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles; Elsevier Science; European Journal Of Pharmaceutical Sciences; 119; 7-2018; 135-146 0928-0987 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/92597 |
identifier_str_mv |
Zelenková, Tereza; Onnainty, Renée; Granero, Gladys Ester; Barresi, Antonello A.; Fissore, Davide; Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles; Elsevier Science; European Journal Of Pharmaceutical Sciences; 119; 7-2018; 135-146 0928-0987 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0928098718301611 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejps.2018.04.006 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614064528949248 |
score |
13.070432 |