Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles

Autores
Zelenková, Tereza; Onnainty, Renée; Granero, Gladys Ester; Barresi, Antonello A.; Fissore, Davide
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed.
Fil: Zelenková, Tereza. Politecnico di Torino; Italia
Fil: Onnainty, Renée. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Barresi, Antonello A.. Politecnico di Torino; Italia
Fil: Fissore, Davide. Politecnico di Torino; Italia
Materia
CHITOSAN
CONFINED IMPINGING JETS MIXER
FREEZE-DRYING
MULTI-INLET VORTEX MIXER
NANOPARTICLES
NANOTECHNOLOGY
SOLVENT DISPLACEMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92597
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92597
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticlesZelenková, TerezaOnnainty, RenéeGranero, Gladys EsterBarresi, Antonello A.Fissore, DavideCHITOSANCONFINED IMPINGING JETS MIXERFREEZE-DRYINGMULTI-INLET VORTEX MIXERNANOPARTICLESNANOTECHNOLOGYSOLVENT DISPLACEMENThttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed.Fil: Zelenková, Tereza. Politecnico di Torino; ItaliaFil: Onnainty, Renée. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Barresi, Antonello A.. Politecnico di Torino; ItaliaFil: Fissore, Davide. Politecnico di Torino; ItaliaElsevier Science2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92597Zelenková, Tereza; Onnainty, Renée; Granero, Gladys Ester; Barresi, Antonello A.; Fissore, Davide; Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles; Elsevier Science; European Journal Of Pharmaceutical Sciences; 119; 7-2018; 135-1460928-0987CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0928098718301611info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejps.2018.04.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:44:04Zoai:ri.conicet.gov.ar:11336/92597instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:44:05.213CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
title Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
spellingShingle Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
Zelenková, Tereza
CHITOSAN
CONFINED IMPINGING JETS MIXER
FREEZE-DRYING
MULTI-INLET VORTEX MIXER
NANOPARTICLES
NANOTECHNOLOGY
SOLVENT DISPLACEMENT
title_short Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
title_full Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
title_fullStr Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
title_full_unstemmed Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
title_sort Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
dc.creator.none.fl_str_mv Zelenková, Tereza
Onnainty, Renée
Granero, Gladys Ester
Barresi, Antonello A.
Fissore, Davide
author Zelenková, Tereza
author_facet Zelenková, Tereza
Onnainty, Renée
Granero, Gladys Ester
Barresi, Antonello A.
Fissore, Davide
author_role author
author2 Onnainty, Renée
Granero, Gladys Ester
Barresi, Antonello A.
Fissore, Davide
author2_role author
author
author
author
dc.subject.none.fl_str_mv CHITOSAN
CONFINED IMPINGING JETS MIXER
FREEZE-DRYING
MULTI-INLET VORTEX MIXER
NANOPARTICLES
NANOTECHNOLOGY
SOLVENT DISPLACEMENT
topic CHITOSAN
CONFINED IMPINGING JETS MIXER
FREEZE-DRYING
MULTI-INLET VORTEX MIXER
NANOPARTICLES
NANOTECHNOLOGY
SOLVENT DISPLACEMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed.
Fil: Zelenková, Tereza. Politecnico di Torino; Italia
Fil: Onnainty, Renée. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Barresi, Antonello A.. Politecnico di Torino; Italia
Fil: Fissore, Davide. Politecnico di Torino; Italia
description This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92597
Zelenková, Tereza; Onnainty, Renée; Granero, Gladys Ester; Barresi, Antonello A.; Fissore, Davide; Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles; Elsevier Science; European Journal Of Pharmaceutical Sciences; 119; 7-2018; 135-146
0928-0987
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92597
identifier_str_mv Zelenková, Tereza; Onnainty, Renée; Granero, Gladys Ester; Barresi, Antonello A.; Fissore, Davide; Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles; Elsevier Science; European Journal Of Pharmaceutical Sciences; 119; 7-2018; 135-146
0928-0987
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0928098718301611
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejps.2018.04.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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