Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection

Autores
Ferrer Navarro, Mario; Planell, Raquel; Yero, Daniel; Mongiardini, Elias Javier; Torrent, Gerard; Huedo, Pol; Martinez, Paula; Roher, Nerea; Mackenzie, Simon; Gibert, Isidre; Daura, Xavier
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little is known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates the study of pathogenicity and virulence factors. Here, we present a strategy to identify such factors in new clinical isolates of S. maltophilia, incorporating an adultzebrafish model of S. maltophilia infection to evaluate relative virulence coupled to 2D difference gel electrophoresis to explore underlying differences in protein expression. In this study we report upon three recent clinical isolates and use the collection strain ATCC13637 as a reference. The adult-zebrafish model shows discrimination capacity, i.e. from very low to very high mortality rates, with clinical symptoms very similar to those observed in natural S. maltophilia infections in fish. Strain virulence correlates with resistance to human serum, in agreement with previous studies in mouse and rat and therefore supporting zebrafish as a replacement model. Despite its clinical origin, the collection strain ATCC13637 showed obvious signs of attenuation in zebrafish, with null mortality. Multilocus-sequence-typing analysis revealed that the most virulent strains, UV74 and M30, exhibit the strongest genetic similitude. Differential proteomic analysis led to the identification of 38 proteins with significantly different abundance in the three clinical strains relative to the reference strain. Orthologs of several of these proteins have been already reported to have a role in pathogenesis, virulence or resistance mechanisms thus supporting our strategy. Proof of concept is further provided by protein Ax21, whose abundance is shown here to be directly proportional to mortality in the zebrafish infection model. Indeed, recent studies have demonstrated that this protein is a quorum-sensing-related virulence factor.
Fil: Ferrer Navarro, Mario. Universitat Autònoma de Barcelona; España
Fil: Planell, Raquel. Universitat Autònoma de Barcelona; España
Fil: Yero, Daniel. Universitat Autònoma de Barcelona; España
Fil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet -La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universitat Autònoma de Barcelona; España
Fil: Torrent, Gerard. Universitat Autònoma de Barcelona; España
Fil: Huedo, Pol. Universitat Autònoma de Barcelona; España
Fil: Martinez, Paula. Universitat Autònoma de Barcelona; España
Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España
Fil: Mackenzie, Simon. Universitat Autònoma de Barcelona; España. University of Stirling; Reino Unido
Fil: Gibert, Isidre. Universitat Autònoma de Barcelona; España
Fil: Daura, Xavier. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España
Materia
Stenotrophomonas
Oportunista
Multiresistencia
Proteomica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22286
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22286
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infectionFerrer Navarro, MarioPlanell, RaquelYero, DanielMongiardini, Elias JavierTorrent, GerardHuedo, PolMartinez, PaulaRoher, NereaMackenzie, SimonGibert, IsidreDaura, XavierStenotrophomonasOportunistaMultiresistenciaProteomicahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little is known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates the study of pathogenicity and virulence factors. Here, we present a strategy to identify such factors in new clinical isolates of S. maltophilia, incorporating an adultzebrafish model of S. maltophilia infection to evaluate relative virulence coupled to 2D difference gel electrophoresis to explore underlying differences in protein expression. In this study we report upon three recent clinical isolates and use the collection strain ATCC13637 as a reference. The adult-zebrafish model shows discrimination capacity, i.e. from very low to very high mortality rates, with clinical symptoms very similar to those observed in natural S. maltophilia infections in fish. Strain virulence correlates with resistance to human serum, in agreement with previous studies in mouse and rat and therefore supporting zebrafish as a replacement model. Despite its clinical origin, the collection strain ATCC13637 showed obvious signs of attenuation in zebrafish, with null mortality. Multilocus-sequence-typing analysis revealed that the most virulent strains, UV74 and M30, exhibit the strongest genetic similitude. Differential proteomic analysis led to the identification of 38 proteins with significantly different abundance in the three clinical strains relative to the reference strain. Orthologs of several of these proteins have been already reported to have a role in pathogenesis, virulence or resistance mechanisms thus supporting our strategy. Proof of concept is further provided by protein Ax21, whose abundance is shown here to be directly proportional to mortality in the zebrafish infection model. Indeed, recent studies have demonstrated that this protein is a quorum-sensing-related virulence factor.Fil: Ferrer Navarro, Mario. Universitat Autònoma de Barcelona; EspañaFil: Planell, Raquel. Universitat Autònoma de Barcelona; EspañaFil: Yero, Daniel. Universitat Autònoma de Barcelona; EspañaFil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet -La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universitat Autònoma de Barcelona; EspañaFil: Torrent, Gerard. Universitat Autònoma de Barcelona; EspañaFil: Huedo, Pol. Universitat Autònoma de Barcelona; EspañaFil: Martinez, Paula. Universitat Autònoma de Barcelona; EspañaFil: Roher, Nerea. Universitat Autònoma de Barcelona; EspañaFil: Mackenzie, Simon. Universitat Autònoma de Barcelona; España. University of Stirling; Reino UnidoFil: Gibert, Isidre. Universitat Autònoma de Barcelona; EspañaFil: Daura, Xavier. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; EspañaPublic Library Science2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22286Ferrer Navarro, Mario; Planell, Raquel; Yero, Daniel; Mongiardini, Elias Javier; Torrent, Gerard; et al.; Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection; Public Library Science; Plos One; 8; 6; 6-2013; 1-10; e672071932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0067207info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067207info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:16:30Zoai:ri.conicet.gov.ar:11336/22286instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:16:30.395CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
title Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
spellingShingle Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
Ferrer Navarro, Mario
Stenotrophomonas
Oportunista
Multiresistencia
Proteomica
title_short Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
title_full Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
title_fullStr Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
title_full_unstemmed Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
title_sort Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection
dc.creator.none.fl_str_mv Ferrer Navarro, Mario
Planell, Raquel
Yero, Daniel
Mongiardini, Elias Javier
Torrent, Gerard
Huedo, Pol
Martinez, Paula
Roher, Nerea
Mackenzie, Simon
Gibert, Isidre
Daura, Xavier
author Ferrer Navarro, Mario
author_facet Ferrer Navarro, Mario
Planell, Raquel
Yero, Daniel
Mongiardini, Elias Javier
Torrent, Gerard
Huedo, Pol
Martinez, Paula
Roher, Nerea
Mackenzie, Simon
Gibert, Isidre
Daura, Xavier
author_role author
author2 Planell, Raquel
Yero, Daniel
Mongiardini, Elias Javier
Torrent, Gerard
Huedo, Pol
Martinez, Paula
Roher, Nerea
Mackenzie, Simon
Gibert, Isidre
Daura, Xavier
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Stenotrophomonas
Oportunista
Multiresistencia
Proteomica
topic Stenotrophomonas
Oportunista
Multiresistencia
Proteomica
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Stenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little is known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates the study of pathogenicity and virulence factors. Here, we present a strategy to identify such factors in new clinical isolates of S. maltophilia, incorporating an adultzebrafish model of S. maltophilia infection to evaluate relative virulence coupled to 2D difference gel electrophoresis to explore underlying differences in protein expression. In this study we report upon three recent clinical isolates and use the collection strain ATCC13637 as a reference. The adult-zebrafish model shows discrimination capacity, i.e. from very low to very high mortality rates, with clinical symptoms very similar to those observed in natural S. maltophilia infections in fish. Strain virulence correlates with resistance to human serum, in agreement with previous studies in mouse and rat and therefore supporting zebrafish as a replacement model. Despite its clinical origin, the collection strain ATCC13637 showed obvious signs of attenuation in zebrafish, with null mortality. Multilocus-sequence-typing analysis revealed that the most virulent strains, UV74 and M30, exhibit the strongest genetic similitude. Differential proteomic analysis led to the identification of 38 proteins with significantly different abundance in the three clinical strains relative to the reference strain. Orthologs of several of these proteins have been already reported to have a role in pathogenesis, virulence or resistance mechanisms thus supporting our strategy. Proof of concept is further provided by protein Ax21, whose abundance is shown here to be directly proportional to mortality in the zebrafish infection model. Indeed, recent studies have demonstrated that this protein is a quorum-sensing-related virulence factor.
Fil: Ferrer Navarro, Mario. Universitat Autònoma de Barcelona; España
Fil: Planell, Raquel. Universitat Autònoma de Barcelona; España
Fil: Yero, Daniel. Universitat Autònoma de Barcelona; España
Fil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet -La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universitat Autònoma de Barcelona; España
Fil: Torrent, Gerard. Universitat Autònoma de Barcelona; España
Fil: Huedo, Pol. Universitat Autònoma de Barcelona; España
Fil: Martinez, Paula. Universitat Autònoma de Barcelona; España
Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España
Fil: Mackenzie, Simon. Universitat Autònoma de Barcelona; España. University of Stirling; Reino Unido
Fil: Gibert, Isidre. Universitat Autònoma de Barcelona; España
Fil: Daura, Xavier. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España
description Stenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little is known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates the study of pathogenicity and virulence factors. Here, we present a strategy to identify such factors in new clinical isolates of S. maltophilia, incorporating an adultzebrafish model of S. maltophilia infection to evaluate relative virulence coupled to 2D difference gel electrophoresis to explore underlying differences in protein expression. In this study we report upon three recent clinical isolates and use the collection strain ATCC13637 as a reference. The adult-zebrafish model shows discrimination capacity, i.e. from very low to very high mortality rates, with clinical symptoms very similar to those observed in natural S. maltophilia infections in fish. Strain virulence correlates with resistance to human serum, in agreement with previous studies in mouse and rat and therefore supporting zebrafish as a replacement model. Despite its clinical origin, the collection strain ATCC13637 showed obvious signs of attenuation in zebrafish, with null mortality. Multilocus-sequence-typing analysis revealed that the most virulent strains, UV74 and M30, exhibit the strongest genetic similitude. Differential proteomic analysis led to the identification of 38 proteins with significantly different abundance in the three clinical strains relative to the reference strain. Orthologs of several of these proteins have been already reported to have a role in pathogenesis, virulence or resistance mechanisms thus supporting our strategy. Proof of concept is further provided by protein Ax21, whose abundance is shown here to be directly proportional to mortality in the zebrafish infection model. Indeed, recent studies have demonstrated that this protein is a quorum-sensing-related virulence factor.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22286
Ferrer Navarro, Mario; Planell, Raquel; Yero, Daniel; Mongiardini, Elias Javier; Torrent, Gerard; et al.; Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection; Public Library Science; Plos One; 8; 6; 6-2013; 1-10; e67207
1932-6203
url http://hdl.handle.net/11336/22286
identifier_str_mv Ferrer Navarro, Mario; Planell, Raquel; Yero, Daniel; Mongiardini, Elias Javier; Torrent, Gerard; et al.; Abundance of the quorum-sensing factor Ax21 in four strains of Stenotrophomonas maltophilia correlates with mortality rate in a new zebrafish model of infection; Public Library Science; Plos One; 8; 6; 6-2013; 1-10; e67207
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0067207
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067207
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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