Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques

Autores
Ferreyra Maillard, Anike Paula Virginia; Gonçalves, Sónia; Santos, Nuno C.; López de Mishima, Beatriz A.; Dalmasso, Pablo Roberto; Hollmann, Axel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of silver nanoparticles (AgNPs) with their novel and distinct physical, chemical, and biological properties, has proven to be an alternative for the development of new antibacterial agents. In particular, the possibility to generate AgNPs coated with novel capping agents, such as phytomolecules obtained via a green synthesis (G-AgNPs), is attracting great attention in scientific research. Recently, we showed that membrane interactions seem to be involved in the antibacterial activity of AgNPs obtained via a green chemical synthesis using the aqueous leaf extract of chicory (Cichorium intybus L.). Furthermore, we observed that these G-AgNPs exhibited higher antibacterial activity than those obtained by chemical synthesis. In order to achieve the green AgNPs mode of action as well as their cellular target, we aimed to study the antibacterial activity of this novel green AgNPs against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The effect of the G-AgNPs on the bacterial surface was first evaluated by zeta potential measurements and correlated with direct plate count agar method. Afterwards, atomic force microscopy was applied to directly unravel the effects of these G-AgNPs on bacterial envelopes. Overall, the data obtained in this study seems correlate with a multi-step mechanism by which G-AgNPs-lipid membrane interactions is the first step prior to membrane disruption, resulting in antibacterial activity.
Fil: Ferreyra Maillard, Anike Paula Virginia. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Gonçalves, Sónia. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Santos, Nuno C.. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: López de Mishima, Beatriz A.. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Dalmasso, Pablo Roberto. Universidad Tecnologica Nacional. Facultad Regional Cordoba. Departamento de Ingenieria Quimica. Centro de Investigacion y Transferencia En Ingenieria Quimica.; Argentina
Fil: Hollmann, Axel. Universidad Nacional de Quilmes; Argentina. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
Materia
ANTIMICROBIAL ACTIVITY
ATOMIC FORCE MICROSCOPY
GREEN SYNTHESIS
LIPID INTERACTION
SILVER NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147669
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/147669
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniquesFerreyra Maillard, Anike Paula VirginiaGonçalves, SóniaSantos, Nuno C.López de Mishima, Beatriz A.Dalmasso, Pablo RobertoHollmann, AxelANTIMICROBIAL ACTIVITYATOMIC FORCE MICROSCOPYGREEN SYNTHESISLIPID INTERACTIONSILVER NANOPARTICLEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2The use of silver nanoparticles (AgNPs) with their novel and distinct physical, chemical, and biological properties, has proven to be an alternative for the development of new antibacterial agents. In particular, the possibility to generate AgNPs coated with novel capping agents, such as phytomolecules obtained via a green synthesis (G-AgNPs), is attracting great attention in scientific research. Recently, we showed that membrane interactions seem to be involved in the antibacterial activity of AgNPs obtained via a green chemical synthesis using the aqueous leaf extract of chicory (Cichorium intybus L.). Furthermore, we observed that these G-AgNPs exhibited higher antibacterial activity than those obtained by chemical synthesis. In order to achieve the green AgNPs mode of action as well as their cellular target, we aimed to study the antibacterial activity of this novel green AgNPs against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The effect of the G-AgNPs on the bacterial surface was first evaluated by zeta potential measurements and correlated with direct plate count agar method. Afterwards, atomic force microscopy was applied to directly unravel the effects of these G-AgNPs on bacterial envelopes. Overall, the data obtained in this study seems correlate with a multi-step mechanism by which G-AgNPs-lipid membrane interactions is the first step prior to membrane disruption, resulting in antibacterial activity.Fil: Ferreyra Maillard, Anike Paula Virginia. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Gonçalves, Sónia. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Santos, Nuno C.. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: López de Mishima, Beatriz A.. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Dalmasso, Pablo Roberto. Universidad Tecnologica Nacional. Facultad Regional Cordoba. Departamento de Ingenieria Quimica. Centro de Investigacion y Transferencia En Ingenieria Quimica.; ArgentinaFil: Hollmann, Axel. Universidad Nacional de Quilmes; Argentina. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; ArgentinaElsevier Science2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147669Ferreyra Maillard, Anike Paula Virginia; Gonçalves, Sónia; Santos, Nuno C.; López de Mishima, Beatriz A.; Dalmasso, Pablo Roberto; et al.; Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1861; 6; 6-2019; 1-70005-2736CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273619300616info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2019.03.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:02Zoai:ri.conicet.gov.ar:11336/147669instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:02.523CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques
title Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques
spellingShingle Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques
Ferreyra Maillard, Anike Paula Virginia
ANTIMICROBIAL ACTIVITY
ATOMIC FORCE MICROSCOPY
GREEN SYNTHESIS
LIPID INTERACTION
SILVER NANOPARTICLES
title_short Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques
title_full Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques
title_fullStr Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques
title_full_unstemmed Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques
title_sort Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques
dc.creator.none.fl_str_mv Ferreyra Maillard, Anike Paula Virginia
Gonçalves, Sónia
Santos, Nuno C.
López de Mishima, Beatriz A.
Dalmasso, Pablo Roberto
Hollmann, Axel
author Ferreyra Maillard, Anike Paula Virginia
author_facet Ferreyra Maillard, Anike Paula Virginia
Gonçalves, Sónia
Santos, Nuno C.
López de Mishima, Beatriz A.
Dalmasso, Pablo Roberto
Hollmann, Axel
author_role author
author2 Gonçalves, Sónia
Santos, Nuno C.
López de Mishima, Beatriz A.
Dalmasso, Pablo Roberto
Hollmann, Axel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL ACTIVITY
ATOMIC FORCE MICROSCOPY
GREEN SYNTHESIS
LIPID INTERACTION
SILVER NANOPARTICLES
topic ANTIMICROBIAL ACTIVITY
ATOMIC FORCE MICROSCOPY
GREEN SYNTHESIS
LIPID INTERACTION
SILVER NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The use of silver nanoparticles (AgNPs) with their novel and distinct physical, chemical, and biological properties, has proven to be an alternative for the development of new antibacterial agents. In particular, the possibility to generate AgNPs coated with novel capping agents, such as phytomolecules obtained via a green synthesis (G-AgNPs), is attracting great attention in scientific research. Recently, we showed that membrane interactions seem to be involved in the antibacterial activity of AgNPs obtained via a green chemical synthesis using the aqueous leaf extract of chicory (Cichorium intybus L.). Furthermore, we observed that these G-AgNPs exhibited higher antibacterial activity than those obtained by chemical synthesis. In order to achieve the green AgNPs mode of action as well as their cellular target, we aimed to study the antibacterial activity of this novel green AgNPs against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The effect of the G-AgNPs on the bacterial surface was first evaluated by zeta potential measurements and correlated with direct plate count agar method. Afterwards, atomic force microscopy was applied to directly unravel the effects of these G-AgNPs on bacterial envelopes. Overall, the data obtained in this study seems correlate with a multi-step mechanism by which G-AgNPs-lipid membrane interactions is the first step prior to membrane disruption, resulting in antibacterial activity.
Fil: Ferreyra Maillard, Anike Paula Virginia. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Gonçalves, Sónia. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Santos, Nuno C.. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: López de Mishima, Beatriz A.. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Dalmasso, Pablo Roberto. Universidad Tecnologica Nacional. Facultad Regional Cordoba. Departamento de Ingenieria Quimica. Centro de Investigacion y Transferencia En Ingenieria Quimica.; Argentina
Fil: Hollmann, Axel. Universidad Nacional de Quilmes; Argentina. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
description The use of silver nanoparticles (AgNPs) with their novel and distinct physical, chemical, and biological properties, has proven to be an alternative for the development of new antibacterial agents. In particular, the possibility to generate AgNPs coated with novel capping agents, such as phytomolecules obtained via a green synthesis (G-AgNPs), is attracting great attention in scientific research. Recently, we showed that membrane interactions seem to be involved in the antibacterial activity of AgNPs obtained via a green chemical synthesis using the aqueous leaf extract of chicory (Cichorium intybus L.). Furthermore, we observed that these G-AgNPs exhibited higher antibacterial activity than those obtained by chemical synthesis. In order to achieve the green AgNPs mode of action as well as their cellular target, we aimed to study the antibacterial activity of this novel green AgNPs against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The effect of the G-AgNPs on the bacterial surface was first evaluated by zeta potential measurements and correlated with direct plate count agar method. Afterwards, atomic force microscopy was applied to directly unravel the effects of these G-AgNPs on bacterial envelopes. Overall, the data obtained in this study seems correlate with a multi-step mechanism by which G-AgNPs-lipid membrane interactions is the first step prior to membrane disruption, resulting in antibacterial activity.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147669
Ferreyra Maillard, Anike Paula Virginia; Gonçalves, Sónia; Santos, Nuno C.; López de Mishima, Beatriz A.; Dalmasso, Pablo Roberto; et al.; Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1861; 6; 6-2019; 1-7
0005-2736
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147669
identifier_str_mv Ferreyra Maillard, Anike Paula Virginia; Gonçalves, Sónia; Santos, Nuno C.; López de Mishima, Beatriz A.; Dalmasso, Pablo Roberto; et al.; Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1861; 6; 6-2019; 1-7
0005-2736
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273619300616
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2019.03.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781379389423616
score 12.982451

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