Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord

Autores
Rodrigues de Amorim, Miguel Augusto; Garcia Segura, Luis Miguel; Goya, Rodolfo Gustavo; Portiansky, Enrique Leo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
PTEN is a tumor suppressor gene known to play an important role in the regulation of cell size. In this study we compared PTEN expression in the spinal cord of young (5 mo.) versus aged (32 mo.) female rats and correlated them with alterations in neuron size and morphology in the same animals. Total and phosphorylated PTEN (pPTEN) as well as its downstream target phosphorylated Akt (pAkt) were assessed by western blotting. Spinal cord neurons were morphometrically characterized. Total PTEN, pPTEN and total Akt expression were significantly higher in young rats than in aged animals. Expression of pAkt was stronger in aged animals. A significant increase in neuronal size was observed in large motoneurons of aged as compared with young rats. Our data show that in the spinal cord of rats, neuronal PTEN expression diminishes with advanced age while neuronal size increases. These results suggest that in the spinal cord, an age-related reduction in PTEN and increase of pAkt expression may be involved in the progressive enlargement of neurons.
Fil: Rodrigues de Amorim, Miguel Augusto. Consejo Superior de Investigaciones Científicas; España
Fil: Garcia Segura, Luis Miguel. Consejo Superior de Investigaciones Científicas; España
Fil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
AGING
MOTONEURONS
NEURONAL SIZE
PROTEIN PHOSPHATASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242454
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cordRodrigues de Amorim, Miguel AugustoGarcia Segura, Luis MiguelGoya, Rodolfo GustavoPortiansky, Enrique LeoAGINGMOTONEURONSNEURONAL SIZEPROTEIN PHOSPHATASEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3PTEN is a tumor suppressor gene known to play an important role in the regulation of cell size. In this study we compared PTEN expression in the spinal cord of young (5 mo.) versus aged (32 mo.) female rats and correlated them with alterations in neuron size and morphology in the same animals. Total and phosphorylated PTEN (pPTEN) as well as its downstream target phosphorylated Akt (pAkt) were assessed by western blotting. Spinal cord neurons were morphometrically characterized. Total PTEN, pPTEN and total Akt expression were significantly higher in young rats than in aged animals. Expression of pAkt was stronger in aged animals. A significant increase in neuronal size was observed in large motoneurons of aged as compared with young rats. Our data show that in the spinal cord of rats, neuronal PTEN expression diminishes with advanced age while neuronal size increases. These results suggest that in the spinal cord, an age-related reduction in PTEN and increase of pAkt expression may be involved in the progressive enlargement of neurons.Fil: Rodrigues de Amorim, Miguel Augusto. Consejo Superior de Investigaciones Científicas; EspañaFil: Garcia Segura, Luis Miguel. Consejo Superior de Investigaciones Científicas; EspañaFil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaPergamon-Elsevier Science Ltd2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242454Rodrigues de Amorim, Miguel Augusto; Garcia Segura, Luis Miguel; Goya, Rodolfo Gustavo; Portiansky, Enrique Leo; Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 45; 6; 6-2010; 457-4630531-5565CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0531556510001294info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exger.2010.03.015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:50Zoai:ri.conicet.gov.ar:11336/242454instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:50.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
spellingShingle Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
Rodrigues de Amorim, Miguel Augusto
AGING
MOTONEURONS
NEURONAL SIZE
PROTEIN PHOSPHATASE
title_short Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_full Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_fullStr Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_full_unstemmed Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
title_sort Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord
dc.creator.none.fl_str_mv Rodrigues de Amorim, Miguel Augusto
Garcia Segura, Luis Miguel
Goya, Rodolfo Gustavo
Portiansky, Enrique Leo
author Rodrigues de Amorim, Miguel Augusto
author_facet Rodrigues de Amorim, Miguel Augusto
Garcia Segura, Luis Miguel
Goya, Rodolfo Gustavo
Portiansky, Enrique Leo
author_role author
author2 Garcia Segura, Luis Miguel
Goya, Rodolfo Gustavo
Portiansky, Enrique Leo
author2_role author
author
author
dc.subject.none.fl_str_mv AGING
MOTONEURONS
NEURONAL SIZE
PROTEIN PHOSPHATASE
topic AGING
MOTONEURONS
NEURONAL SIZE
PROTEIN PHOSPHATASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv PTEN is a tumor suppressor gene known to play an important role in the regulation of cell size. In this study we compared PTEN expression in the spinal cord of young (5 mo.) versus aged (32 mo.) female rats and correlated them with alterations in neuron size and morphology in the same animals. Total and phosphorylated PTEN (pPTEN) as well as its downstream target phosphorylated Akt (pAkt) were assessed by western blotting. Spinal cord neurons were morphometrically characterized. Total PTEN, pPTEN and total Akt expression were significantly higher in young rats than in aged animals. Expression of pAkt was stronger in aged animals. A significant increase in neuronal size was observed in large motoneurons of aged as compared with young rats. Our data show that in the spinal cord of rats, neuronal PTEN expression diminishes with advanced age while neuronal size increases. These results suggest that in the spinal cord, an age-related reduction in PTEN and increase of pAkt expression may be involved in the progressive enlargement of neurons.
Fil: Rodrigues de Amorim, Miguel Augusto. Consejo Superior de Investigaciones Científicas; España
Fil: Garcia Segura, Luis Miguel. Consejo Superior de Investigaciones Científicas; España
Fil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description PTEN is a tumor suppressor gene known to play an important role in the regulation of cell size. In this study we compared PTEN expression in the spinal cord of young (5 mo.) versus aged (32 mo.) female rats and correlated them with alterations in neuron size and morphology in the same animals. Total and phosphorylated PTEN (pPTEN) as well as its downstream target phosphorylated Akt (pAkt) were assessed by western blotting. Spinal cord neurons were morphometrically characterized. Total PTEN, pPTEN and total Akt expression were significantly higher in young rats than in aged animals. Expression of pAkt was stronger in aged animals. A significant increase in neuronal size was observed in large motoneurons of aged as compared with young rats. Our data show that in the spinal cord of rats, neuronal PTEN expression diminishes with advanced age while neuronal size increases. These results suggest that in the spinal cord, an age-related reduction in PTEN and increase of pAkt expression may be involved in the progressive enlargement of neurons.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242454
Rodrigues de Amorim, Miguel Augusto; Garcia Segura, Luis Miguel; Goya, Rodolfo Gustavo; Portiansky, Enrique Leo; Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 45; 6; 6-2010; 457-463
0531-5565
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242454
identifier_str_mv Rodrigues de Amorim, Miguel Augusto; Garcia Segura, Luis Miguel; Goya, Rodolfo Gustavo; Portiansky, Enrique Leo; Decrease in PTEN and increase in Akt expression and neuron size in aged rat spinal cord; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 45; 6; 6-2010; 457-463
0531-5565
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0531556510001294
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exger.2010.03.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.891075

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