Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina

Autores
Reinoso, Elina Beatríz; El Sayed, A.; Lämmler, C.; Bogni, C.; Zschöck, M.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to characterize genotypically 45 Staphylococcus aureus strains isolated from humans, bovine subclinical mastitis and food samples in Argentina by rep-PCR and PCR amplification of virulence genes. Resistances to various antibiotics could be observed for the human S. aureus, less pronounced for the bovine strains, but not for the eight S. aureus isolated from food samples. The strains could be classified genotypically by rep-PCR and by amplification of the genes encoding protein A, coagulase, clumping factor, the collagen adhesin domains A and B, capsular polysaccharide 5 and 8, the accessory gene regulator agr classes I to III, and the S. aureus gene regulator sae. rep-PCR analyses and the different gene patterns revealed that the strains could be divided into seven groups mostly matching with the origin of the isolates. The present study describes genotypic variations of S. aureus strains isolated from different origins in Argentina. The study provides a valuable insight into molecular specificities of this important pathogen.
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: El Sayed, A.. Justus Liebig Universitat Giessen; Alemania
Fil: Lämmler, C.. Justus Liebig Universitat Giessen; Alemania
Fil: Bogni, C.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Zschöck, M.. No especifíca;
Materia
STAPHYLOCOCCUS COAGULASA NEGATIVOS
HUMAN
BOVINE
FOOD SAMPLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242412
Acceder
id CONICETDig_66e364c8bd1d211410487ac34826197a
oai_identifier_str oai:ri.conicet.gov.ar:11336/242412
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in ArgentinaReinoso, Elina BeatrízEl Sayed, A.Lämmler, C.Bogni, C.Zschöck, M.STAPHYLOCOCCUS COAGULASA NEGATIVOSHUMANBOVINEFOOD SAMPLEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the present study was to characterize genotypically 45 Staphylococcus aureus strains isolated from humans, bovine subclinical mastitis and food samples in Argentina by rep-PCR and PCR amplification of virulence genes. Resistances to various antibiotics could be observed for the human S. aureus, less pronounced for the bovine strains, but not for the eight S. aureus isolated from food samples. The strains could be classified genotypically by rep-PCR and by amplification of the genes encoding protein A, coagulase, clumping factor, the collagen adhesin domains A and B, capsular polysaccharide 5 and 8, the accessory gene regulator agr classes I to III, and the S. aureus gene regulator sae. rep-PCR analyses and the different gene patterns revealed that the strains could be divided into seven groups mostly matching with the origin of the isolates. The present study describes genotypic variations of S. aureus strains isolated from different origins in Argentina. The study provides a valuable insight into molecular specificities of this important pathogen.Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: El Sayed, A.. Justus Liebig Universitat Giessen; AlemaniaFil: Lämmler, C.. Justus Liebig Universitat Giessen; AlemaniaFil: Bogni, C.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Zschöck, M.. No especifíca;Elsevier Gmbh2008-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242412Reinoso, Elina Beatríz; El Sayed, A.; Lämmler, C.; Bogni, C.; Zschöck, M.; Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina; Elsevier Gmbh; Microbiological Research; 163; 3; 5-2008; 314-3220944-5013CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S094450130600067X?info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2006.05.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:23Zoai:ri.conicet.gov.ar:11336/242412instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:23.891CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina
title Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina
spellingShingle Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina
Reinoso, Elina Beatríz
STAPHYLOCOCCUS COAGULASA NEGATIVOS
HUMAN
BOVINE
FOOD SAMPLES
title_short Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina
title_full Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina
title_fullStr Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina
title_full_unstemmed Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina
title_sort Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina
dc.creator.none.fl_str_mv Reinoso, Elina Beatríz
El Sayed, A.
Lämmler, C.
Bogni, C.
Zschöck, M.
author Reinoso, Elina Beatríz
author_facet Reinoso, Elina Beatríz
El Sayed, A.
Lämmler, C.
Bogni, C.
Zschöck, M.
author_role author
author2 El Sayed, A.
Lämmler, C.
Bogni, C.
Zschöck, M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv STAPHYLOCOCCUS COAGULASA NEGATIVOS
HUMAN
BOVINE
FOOD SAMPLES
topic STAPHYLOCOCCUS COAGULASA NEGATIVOS
HUMAN
BOVINE
FOOD SAMPLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present study was to characterize genotypically 45 Staphylococcus aureus strains isolated from humans, bovine subclinical mastitis and food samples in Argentina by rep-PCR and PCR amplification of virulence genes. Resistances to various antibiotics could be observed for the human S. aureus, less pronounced for the bovine strains, but not for the eight S. aureus isolated from food samples. The strains could be classified genotypically by rep-PCR and by amplification of the genes encoding protein A, coagulase, clumping factor, the collagen adhesin domains A and B, capsular polysaccharide 5 and 8, the accessory gene regulator agr classes I to III, and the S. aureus gene regulator sae. rep-PCR analyses and the different gene patterns revealed that the strains could be divided into seven groups mostly matching with the origin of the isolates. The present study describes genotypic variations of S. aureus strains isolated from different origins in Argentina. The study provides a valuable insight into molecular specificities of this important pathogen.
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: El Sayed, A.. Justus Liebig Universitat Giessen; Alemania
Fil: Lämmler, C.. Justus Liebig Universitat Giessen; Alemania
Fil: Bogni, C.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Zschöck, M.. No especifíca;
description The aim of the present study was to characterize genotypically 45 Staphylococcus aureus strains isolated from humans, bovine subclinical mastitis and food samples in Argentina by rep-PCR and PCR amplification of virulence genes. Resistances to various antibiotics could be observed for the human S. aureus, less pronounced for the bovine strains, but not for the eight S. aureus isolated from food samples. The strains could be classified genotypically by rep-PCR and by amplification of the genes encoding protein A, coagulase, clumping factor, the collagen adhesin domains A and B, capsular polysaccharide 5 and 8, the accessory gene regulator agr classes I to III, and the S. aureus gene regulator sae. rep-PCR analyses and the different gene patterns revealed that the strains could be divided into seven groups mostly matching with the origin of the isolates. The present study describes genotypic variations of S. aureus strains isolated from different origins in Argentina. The study provides a valuable insight into molecular specificities of this important pathogen.
publishDate 2008
dc.date.none.fl_str_mv 2008-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242412
Reinoso, Elina Beatríz; El Sayed, A.; Lämmler, C.; Bogni, C.; Zschöck, M.; Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina; Elsevier Gmbh; Microbiological Research; 163; 3; 5-2008; 314-322
0944-5013
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242412
identifier_str_mv Reinoso, Elina Beatríz; El Sayed, A.; Lämmler, C.; Bogni, C.; Zschöck, M.; Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina; Elsevier Gmbh; Microbiological Research; 163; 3; 5-2008; 314-322
0944-5013
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S094450130600067X?
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2006.05.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269460074856448
score 13.13397

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