Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica
- Autores
- Arias, Diego Gustavo; Carranza, Pedro Gabriel; Lujan, Hugo Daniel; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The components of the redox metabolism in Entamoeba histolytica have been recently revisited by Arias et al. (Free Radic. Biol. Med. 42:1496-1505; 2007), after the identification and characterization of a thioredoxin-linked system. The present work deals with studies performed for a better understanding of the localization and identification of different components of the redox machinery present in the parasite. The gene encoding for amoebic thioredoxin 8 was cloned and the recombinant protein typified as having properties similar to those of thioredoxin 41. The ability of these thioredoxins and the specific reductase to assemble a system utilizing NADPH to metabolize hydroperoxides in association with a peroxiredoxin has been kinetically characterized. The peroxiredoxin behaved as a typical 2 cysteine enzyme, exhibiting a ping-pong mechanism with hyperbolic saturation kinetics for thioredoxin 8 (Km = 3.8 μM), thioredoxin 41 (Km = 3.1 μM), and tert-butyl hydroperoxide (Km about 35 μM). Moreover, the tandem system involving thioredoxin reductase and either thioredoxin proved to be operative for reducing low molecular weight disulfides, including putative physiological substrates as cystine and oxidized trypanothione. Thioredoxin reductase and thioredoxin 41 (by association also the functional redox system) have been immunolocalized underlying the plasma membrane in Entamoeba histolytica cells. These findings suggest an important role for the metabolic pathway involving thioredoxin as a redox interchanger, which could be critical for the maintenance and virulence of the parasite when exposed to highly toxic reactive oxygen species.
Fil: Arias, Diego Gustavo. Universidad Nacional del Litoral; Argentina
Fil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Lujan, Hugo Daniel. Universidad Católica de Córdoba. Facultad de Medicina; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina - Materia
-
ENTAMOEBA HISTOLYTICA
PEROXIREDOXIN
REDOX BALANCE
THIOLS
THIOREDOXIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/84801
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Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolyticaArias, Diego GustavoCarranza, Pedro GabrielLujan, Hugo DanielIglesias, Alberto AlvaroGuerrero, Sergio AdrianENTAMOEBA HISTOLYTICAPEROXIREDOXINREDOX BALANCETHIOLSTHIOREDOXINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The components of the redox metabolism in Entamoeba histolytica have been recently revisited by Arias et al. (Free Radic. Biol. Med. 42:1496-1505; 2007), after the identification and characterization of a thioredoxin-linked system. The present work deals with studies performed for a better understanding of the localization and identification of different components of the redox machinery present in the parasite. The gene encoding for amoebic thioredoxin 8 was cloned and the recombinant protein typified as having properties similar to those of thioredoxin 41. The ability of these thioredoxins and the specific reductase to assemble a system utilizing NADPH to metabolize hydroperoxides in association with a peroxiredoxin has been kinetically characterized. The peroxiredoxin behaved as a typical 2 cysteine enzyme, exhibiting a ping-pong mechanism with hyperbolic saturation kinetics for thioredoxin 8 (Km = 3.8 μM), thioredoxin 41 (Km = 3.1 μM), and tert-butyl hydroperoxide (Km about 35 μM). Moreover, the tandem system involving thioredoxin reductase and either thioredoxin proved to be operative for reducing low molecular weight disulfides, including putative physiological substrates as cystine and oxidized trypanothione. Thioredoxin reductase and thioredoxin 41 (by association also the functional redox system) have been immunolocalized underlying the plasma membrane in Entamoeba histolytica cells. These findings suggest an important role for the metabolic pathway involving thioredoxin as a redox interchanger, which could be critical for the maintenance and virulence of the parasite when exposed to highly toxic reactive oxygen species.Fil: Arias, Diego Gustavo. Universidad Nacional del Litoral; ArgentinaFil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Lujan, Hugo Daniel. Universidad Católica de Córdoba. Facultad de Medicina; ArgentinaFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaElsevier Science Inc2008-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84801Arias, Diego Gustavo; Carranza, Pedro Gabriel; Lujan, Hugo Daniel; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica; Elsevier Science Inc; Free Radical Biology and Medicine; 45; 1; 7-2008; 32-390891-5849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.elsevier.com/wps/find/journaldescription.cws_home/525469/description#descriptioninfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2008.03.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:17Zoai:ri.conicet.gov.ar:11336/84801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:17.408CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica |
title |
Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica |
spellingShingle |
Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica Arias, Diego Gustavo ENTAMOEBA HISTOLYTICA PEROXIREDOXIN REDOX BALANCE THIOLS THIOREDOXIN |
title_short |
Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica |
title_full |
Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica |
title_fullStr |
Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica |
title_full_unstemmed |
Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica |
title_sort |
Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica |
dc.creator.none.fl_str_mv |
Arias, Diego Gustavo Carranza, Pedro Gabriel Lujan, Hugo Daniel Iglesias, Alberto Alvaro Guerrero, Sergio Adrian |
author |
Arias, Diego Gustavo |
author_facet |
Arias, Diego Gustavo Carranza, Pedro Gabriel Lujan, Hugo Daniel Iglesias, Alberto Alvaro Guerrero, Sergio Adrian |
author_role |
author |
author2 |
Carranza, Pedro Gabriel Lujan, Hugo Daniel Iglesias, Alberto Alvaro Guerrero, Sergio Adrian |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
ENTAMOEBA HISTOLYTICA PEROXIREDOXIN REDOX BALANCE THIOLS THIOREDOXIN |
topic |
ENTAMOEBA HISTOLYTICA PEROXIREDOXIN REDOX BALANCE THIOLS THIOREDOXIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The components of the redox metabolism in Entamoeba histolytica have been recently revisited by Arias et al. (Free Radic. Biol. Med. 42:1496-1505; 2007), after the identification and characterization of a thioredoxin-linked system. The present work deals with studies performed for a better understanding of the localization and identification of different components of the redox machinery present in the parasite. The gene encoding for amoebic thioredoxin 8 was cloned and the recombinant protein typified as having properties similar to those of thioredoxin 41. The ability of these thioredoxins and the specific reductase to assemble a system utilizing NADPH to metabolize hydroperoxides in association with a peroxiredoxin has been kinetically characterized. The peroxiredoxin behaved as a typical 2 cysteine enzyme, exhibiting a ping-pong mechanism with hyperbolic saturation kinetics for thioredoxin 8 (Km = 3.8 μM), thioredoxin 41 (Km = 3.1 μM), and tert-butyl hydroperoxide (Km about 35 μM). Moreover, the tandem system involving thioredoxin reductase and either thioredoxin proved to be operative for reducing low molecular weight disulfides, including putative physiological substrates as cystine and oxidized trypanothione. Thioredoxin reductase and thioredoxin 41 (by association also the functional redox system) have been immunolocalized underlying the plasma membrane in Entamoeba histolytica cells. These findings suggest an important role for the metabolic pathway involving thioredoxin as a redox interchanger, which could be critical for the maintenance and virulence of the parasite when exposed to highly toxic reactive oxygen species. Fil: Arias, Diego Gustavo. Universidad Nacional del Litoral; Argentina Fil: Carranza, Pedro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Lujan, Hugo Daniel. Universidad Católica de Córdoba. Facultad de Medicina; Argentina Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina |
description |
The components of the redox metabolism in Entamoeba histolytica have been recently revisited by Arias et al. (Free Radic. Biol. Med. 42:1496-1505; 2007), after the identification and characterization of a thioredoxin-linked system. The present work deals with studies performed for a better understanding of the localization and identification of different components of the redox machinery present in the parasite. The gene encoding for amoebic thioredoxin 8 was cloned and the recombinant protein typified as having properties similar to those of thioredoxin 41. The ability of these thioredoxins and the specific reductase to assemble a system utilizing NADPH to metabolize hydroperoxides in association with a peroxiredoxin has been kinetically characterized. The peroxiredoxin behaved as a typical 2 cysteine enzyme, exhibiting a ping-pong mechanism with hyperbolic saturation kinetics for thioredoxin 8 (Km = 3.8 μM), thioredoxin 41 (Km = 3.1 μM), and tert-butyl hydroperoxide (Km about 35 μM). Moreover, the tandem system involving thioredoxin reductase and either thioredoxin proved to be operative for reducing low molecular weight disulfides, including putative physiological substrates as cystine and oxidized trypanothione. Thioredoxin reductase and thioredoxin 41 (by association also the functional redox system) have been immunolocalized underlying the plasma membrane in Entamoeba histolytica cells. These findings suggest an important role for the metabolic pathway involving thioredoxin as a redox interchanger, which could be critical for the maintenance and virulence of the parasite when exposed to highly toxic reactive oxygen species. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/84801 Arias, Diego Gustavo; Carranza, Pedro Gabriel; Lujan, Hugo Daniel; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica; Elsevier Science Inc; Free Radical Biology and Medicine; 45; 1; 7-2008; 32-39 0891-5849 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/84801 |
identifier_str_mv |
Arias, Diego Gustavo; Carranza, Pedro Gabriel; Lujan, Hugo Daniel; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; Immunolocalization and enzymatic functional characterization of the thioredoxin system in Entamoeba histolytica; Elsevier Science Inc; Free Radical Biology and Medicine; 45; 1; 7-2008; 32-39 0891-5849 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.elsevier.com/wps/find/journaldescription.cws_home/525469/description#description info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2008.03.008 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Inc |
publisher.none.fl_str_mv |
Elsevier Science Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269453241286656 |
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13.13397 |