Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro

Autores
Santoro, Valeria Maricel; Nievas, Fiorela Lujan; Zygadlo, Julio Alberto; Giordano, Walter Fabian; Banchio, Erika
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was experimentally supplemented with the auxin 4-indol-3-ylbutyric acid (IBA) and the cytokinin 6-benzylaminopurine (BAP) individually and in combination. Supplementation with BAP alone resulted in the highest values for root length, root dry weight, shoot length, and numbers of nodes, leaves, and ramifications. Treatment with IBA alone or with IBA + BAP resulted in a ~50% increase in shoot fresh weight. The production of secondary metabolites was affected only by the addition of cytokinin, which resulted in a ~40% increase in the total yield of essential oils (EOs). Similar trends were observed for yields of the major EO components (menthone, menthol, pulegone, and menthofuran). Our findings demonstrate that the application of growth regulators increases EO production and biomass concomitantly in an herbaceous species rich in commercially valuable terpenes.
Fil: Santoro, Valeria Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nievas, Fiorela Lujan. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zygadlo, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Banchio, Erika. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Micropropagated Plants
Mentha piperita
Growth Regulators
IBA
BAP
Secondary Metabolites
Essential Oils
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22617
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22617
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in VitroSantoro, Valeria MaricelNievas, Fiorela LujanZygadlo, Julio AlbertoGiordano, Walter FabianBanchio, ErikaMicropropagated PlantsMentha piperitaGrowth RegulatorsIBABAPSecondary MetabolitesEssential Oilshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was experimentally supplemented with the auxin 4-indol-3-ylbutyric acid (IBA) and the cytokinin 6-benzylaminopurine (BAP) individually and in combination. Supplementation with BAP alone resulted in the highest values for root length, root dry weight, shoot length, and numbers of nodes, leaves, and ramifications. Treatment with IBA alone or with IBA + BAP resulted in a ~50% increase in shoot fresh weight. The production of secondary metabolites was affected only by the addition of cytokinin, which resulted in a ~40% increase in the total yield of essential oils (EOs). Similar trends were observed for yields of the major EO components (menthone, menthol, pulegone, and menthofuran). Our findings demonstrate that the application of growth regulators increases EO production and biomass concomitantly in an herbaceous species rich in commercially valuable terpenes.Fil: Santoro, Valeria Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nievas, Fiorela Lujan. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zygadlo, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Banchio, Erika. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaScientific Research2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22617Santoro, Valeria Maricel; Nievas, Fiorela Lujan; Zygadlo, Julio Alberto; Giordano, Walter Fabian; Banchio, Erika; Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro; Scientific Research; American Journal of Plant Sciences; 4; 5A; -1-2013; 1-7; 322532158-2742CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4236/ajps.2013.45A008info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/8-2600770_32253.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:22Zoai:ri.conicet.gov.ar:11336/22617instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:22.889CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro
title Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro
spellingShingle Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro
Santoro, Valeria Maricel
Micropropagated Plants
Mentha piperita
Growth Regulators
IBA
BAP
Secondary Metabolites
Essential Oils
title_short Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro
title_full Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro
title_fullStr Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro
title_full_unstemmed Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro
title_sort Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro
dc.creator.none.fl_str_mv Santoro, Valeria Maricel
Nievas, Fiorela Lujan
Zygadlo, Julio Alberto
Giordano, Walter Fabian
Banchio, Erika
author Santoro, Valeria Maricel
author_facet Santoro, Valeria Maricel
Nievas, Fiorela Lujan
Zygadlo, Julio Alberto
Giordano, Walter Fabian
Banchio, Erika
author_role author
author2 Nievas, Fiorela Lujan
Zygadlo, Julio Alberto
Giordano, Walter Fabian
Banchio, Erika
author2_role author
author
author
author
dc.subject.none.fl_str_mv Micropropagated Plants
Mentha piperita
Growth Regulators
IBA
BAP
Secondary Metabolites
Essential Oils
topic Micropropagated Plants
Mentha piperita
Growth Regulators
IBA
BAP
Secondary Metabolites
Essential Oils
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was experimentally supplemented with the auxin 4-indol-3-ylbutyric acid (IBA) and the cytokinin 6-benzylaminopurine (BAP) individually and in combination. Supplementation with BAP alone resulted in the highest values for root length, root dry weight, shoot length, and numbers of nodes, leaves, and ramifications. Treatment with IBA alone or with IBA + BAP resulted in a ~50% increase in shoot fresh weight. The production of secondary metabolites was affected only by the addition of cytokinin, which resulted in a ~40% increase in the total yield of essential oils (EOs). Similar trends were observed for yields of the major EO components (menthone, menthol, pulegone, and menthofuran). Our findings demonstrate that the application of growth regulators increases EO production and biomass concomitantly in an herbaceous species rich in commercially valuable terpenes.
Fil: Santoro, Valeria Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nievas, Fiorela Lujan. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zygadlo, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Banchio, Erika. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The effects of plant growth regulators on peppermint (Mentha piperita) cultured in vitro were studied for the purpose of maximizing growth and essential oil production in micropropagated plants. The basal medium was experimentally supplemented with the auxin 4-indol-3-ylbutyric acid (IBA) and the cytokinin 6-benzylaminopurine (BAP) individually and in combination. Supplementation with BAP alone resulted in the highest values for root length, root dry weight, shoot length, and numbers of nodes, leaves, and ramifications. Treatment with IBA alone or with IBA + BAP resulted in a ~50% increase in shoot fresh weight. The production of secondary metabolites was affected only by the addition of cytokinin, which resulted in a ~40% increase in the total yield of essential oils (EOs). Similar trends were observed for yields of the major EO components (menthone, menthol, pulegone, and menthofuran). Our findings demonstrate that the application of growth regulators increases EO production and biomass concomitantly in an herbaceous species rich in commercially valuable terpenes.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22617
Santoro, Valeria Maricel; Nievas, Fiorela Lujan; Zygadlo, Julio Alberto; Giordano, Walter Fabian; Banchio, Erika; Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro; Scientific Research; American Journal of Plant Sciences; 4; 5A; -1-2013; 1-7; 32253
2158-2742
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22617
identifier_str_mv Santoro, Valeria Maricel; Nievas, Fiorela Lujan; Zygadlo, Julio Alberto; Giordano, Walter Fabian; Banchio, Erika; Effects of Growth Regulators on Biomass and the Production of Secondary Metabolites in Peppermint (Mentha piperita) Micropropagated in Vitro; Scientific Research; American Journal of Plant Sciences; 4; 5A; -1-2013; 1-7; 32253
2158-2742
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/ajps.2013.45A008
info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/8-2600770_32253.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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