Stealth nanocarriers based sterosomes using PEG post-insertion process

Autores
Cieślak, Anna; Wauthoz, Nathalie; Nieto Orellana, Alejandro; Lautram, Nolwenn; Béjaud, Jérôme; Hureaux, José; Lafleur, Michel; Benoit, Jean-Pierre; Salomon, Claudio Javier; Bastiat, Guillaume
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sterosomes (STEs), a new and promising non-phospholipidic liposome platform based on palmitic acid (PA) and cholesterol (Chol) mixtures, need to have polyethylene glycol (PEG) chains grafted to their surface in order to obtain long-circulating nanocarriers in the blood stream. A post-insertion method was chosen to achieve this modification. The post-insertion process of PEG-modified distearoylphosphoethanolamine (DSPE-PEG) was monitored using the zeta potential value of STEs. Various conditions including PEG chain length and the DSPE-PEG/PA-Chol ratio, were explored. Zeta potential of STEs changed from about −40 mV for non-modified STEs to values close to 0 mV by the end of the process, i.e. for PEG-modified STEs. The kinetics of DSPE-PEG insertion and the stability of the resulting PEG-modified STEs were not considerably influenced, within the investigated range, by changes in PEG chain lengths and in DSPE-PEG/PA-Chol proportion. The post-insertion of PEG chains reduced in vitro complement activation as well as in vitro macrophage uptake compared to the non-modified STEs. Moreover, longer blood circulation time in mice was established for PEG-modified STEs intravenously injected compared to non-modified STEs. These results establish that post-insertion process of PEG chains to STEs is a promising strategy for developing long-term circulating drug delivery nanocarriers.
Fil: Cieślak, Anna. University Of Angers; Francia
Fil: Wauthoz, Nathalie. University Of Angers; Francia. Université Libre de Bruxelles; Bélgica
Fil: Nieto Orellana, Alejandro. University Of Angers; Francia
Fil: Lautram, Nolwenn. University Of Angers; Francia
Fil: Béjaud, Jérôme. University Of Angers; Francia
Fil: Hureaux, José. University Of Angers; Francia
Fil: Lafleur, Michel. University of Montreal; Canadá
Fil: Benoit, Jean-Pierre. University Of Angers; Francia
Fil: Salomon, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Bastiat, Guillaume. University Of Angers; Francia
Materia
BIODISTRIBUTION
CH50 EXPERIMENT
MACROPHAGE UPTAKE
PEG
STEROSOMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100753
Acceder
id CONICETDig_63dc9a8ebf4effb6c353b318da203c49
oai_identifier_str oai:ri.conicet.gov.ar:11336/100753
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Stealth nanocarriers based sterosomes using PEG post-insertion processCieślak, AnnaWauthoz, NathalieNieto Orellana, AlejandroLautram, NolwennBéjaud, JérômeHureaux, JoséLafleur, MichelBenoit, Jean-PierreSalomon, Claudio JavierBastiat, GuillaumeBIODISTRIBUTIONCH50 EXPERIMENTMACROPHAGE UPTAKEPEGSTEROSOMEShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Sterosomes (STEs), a new and promising non-phospholipidic liposome platform based on palmitic acid (PA) and cholesterol (Chol) mixtures, need to have polyethylene glycol (PEG) chains grafted to their surface in order to obtain long-circulating nanocarriers in the blood stream. A post-insertion method was chosen to achieve this modification. The post-insertion process of PEG-modified distearoylphosphoethanolamine (DSPE-PEG) was monitored using the zeta potential value of STEs. Various conditions including PEG chain length and the DSPE-PEG/PA-Chol ratio, were explored. Zeta potential of STEs changed from about −40 mV for non-modified STEs to values close to 0 mV by the end of the process, i.e. for PEG-modified STEs. The kinetics of DSPE-PEG insertion and the stability of the resulting PEG-modified STEs were not considerably influenced, within the investigated range, by changes in PEG chain lengths and in DSPE-PEG/PA-Chol proportion. The post-insertion of PEG chains reduced in vitro complement activation as well as in vitro macrophage uptake compared to the non-modified STEs. Moreover, longer blood circulation time in mice was established for PEG-modified STEs intravenously injected compared to non-modified STEs. These results establish that post-insertion process of PEG chains to STEs is a promising strategy for developing long-term circulating drug delivery nanocarriers.Fil: Cieślak, Anna. University Of Angers; FranciaFil: Wauthoz, Nathalie. University Of Angers; Francia. Université Libre de Bruxelles; BélgicaFil: Nieto Orellana, Alejandro. University Of Angers; FranciaFil: Lautram, Nolwenn. University Of Angers; FranciaFil: Béjaud, Jérôme. University Of Angers; FranciaFil: Hureaux, José. University Of Angers; FranciaFil: Lafleur, Michel. University of Montreal; CanadáFil: Benoit, Jean-Pierre. University Of Angers; FranciaFil: Salomon, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Bastiat, Guillaume. University Of Angers; FranciaElsevier Science2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100753Cieślak, Anna; Wauthoz, Nathalie; Nieto Orellana, Alejandro; Lautram, Nolwenn; Béjaud, Jérôme; et al.; Stealth nanocarriers based sterosomes using PEG post-insertion process; Elsevier Science; European Journal Of Pharmaceutics And Biopharmaceutics; 115; 6-2017; 31-380939-6411CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0939641117301996info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejpb.2017.02.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:15:28Zoai:ri.conicet.gov.ar:11336/100753instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:15:28.657CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stealth nanocarriers based sterosomes using PEG post-insertion process
title Stealth nanocarriers based sterosomes using PEG post-insertion process
spellingShingle Stealth nanocarriers based sterosomes using PEG post-insertion process
Cieślak, Anna
BIODISTRIBUTION
CH50 EXPERIMENT
MACROPHAGE UPTAKE
PEG
STEROSOMES
title_short Stealth nanocarriers based sterosomes using PEG post-insertion process
title_full Stealth nanocarriers based sterosomes using PEG post-insertion process
title_fullStr Stealth nanocarriers based sterosomes using PEG post-insertion process
title_full_unstemmed Stealth nanocarriers based sterosomes using PEG post-insertion process
title_sort Stealth nanocarriers based sterosomes using PEG post-insertion process
dc.creator.none.fl_str_mv Cieślak, Anna
Wauthoz, Nathalie
Nieto Orellana, Alejandro
Lautram, Nolwenn
Béjaud, Jérôme
Hureaux, José
Lafleur, Michel
Benoit, Jean-Pierre
Salomon, Claudio Javier
Bastiat, Guillaume
author Cieślak, Anna
author_facet Cieślak, Anna
Wauthoz, Nathalie
Nieto Orellana, Alejandro
Lautram, Nolwenn
Béjaud, Jérôme
Hureaux, José
Lafleur, Michel
Benoit, Jean-Pierre
Salomon, Claudio Javier
Bastiat, Guillaume
author_role author
author2 Wauthoz, Nathalie
Nieto Orellana, Alejandro
Lautram, Nolwenn
Béjaud, Jérôme
Hureaux, José
Lafleur, Michel
Benoit, Jean-Pierre
Salomon, Claudio Javier
Bastiat, Guillaume
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIODISTRIBUTION
CH50 EXPERIMENT
MACROPHAGE UPTAKE
PEG
STEROSOMES
topic BIODISTRIBUTION
CH50 EXPERIMENT
MACROPHAGE UPTAKE
PEG
STEROSOMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Sterosomes (STEs), a new and promising non-phospholipidic liposome platform based on palmitic acid (PA) and cholesterol (Chol) mixtures, need to have polyethylene glycol (PEG) chains grafted to their surface in order to obtain long-circulating nanocarriers in the blood stream. A post-insertion method was chosen to achieve this modification. The post-insertion process of PEG-modified distearoylphosphoethanolamine (DSPE-PEG) was monitored using the zeta potential value of STEs. Various conditions including PEG chain length and the DSPE-PEG/PA-Chol ratio, were explored. Zeta potential of STEs changed from about −40 mV for non-modified STEs to values close to 0 mV by the end of the process, i.e. for PEG-modified STEs. The kinetics of DSPE-PEG insertion and the stability of the resulting PEG-modified STEs were not considerably influenced, within the investigated range, by changes in PEG chain lengths and in DSPE-PEG/PA-Chol proportion. The post-insertion of PEG chains reduced in vitro complement activation as well as in vitro macrophage uptake compared to the non-modified STEs. Moreover, longer blood circulation time in mice was established for PEG-modified STEs intravenously injected compared to non-modified STEs. These results establish that post-insertion process of PEG chains to STEs is a promising strategy for developing long-term circulating drug delivery nanocarriers.
Fil: Cieślak, Anna. University Of Angers; Francia
Fil: Wauthoz, Nathalie. University Of Angers; Francia. Université Libre de Bruxelles; Bélgica
Fil: Nieto Orellana, Alejandro. University Of Angers; Francia
Fil: Lautram, Nolwenn. University Of Angers; Francia
Fil: Béjaud, Jérôme. University Of Angers; Francia
Fil: Hureaux, José. University Of Angers; Francia
Fil: Lafleur, Michel. University of Montreal; Canadá
Fil: Benoit, Jean-Pierre. University Of Angers; Francia
Fil: Salomon, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina
Fil: Bastiat, Guillaume. University Of Angers; Francia
description Sterosomes (STEs), a new and promising non-phospholipidic liposome platform based on palmitic acid (PA) and cholesterol (Chol) mixtures, need to have polyethylene glycol (PEG) chains grafted to their surface in order to obtain long-circulating nanocarriers in the blood stream. A post-insertion method was chosen to achieve this modification. The post-insertion process of PEG-modified distearoylphosphoethanolamine (DSPE-PEG) was monitored using the zeta potential value of STEs. Various conditions including PEG chain length and the DSPE-PEG/PA-Chol ratio, were explored. Zeta potential of STEs changed from about −40 mV for non-modified STEs to values close to 0 mV by the end of the process, i.e. for PEG-modified STEs. The kinetics of DSPE-PEG insertion and the stability of the resulting PEG-modified STEs were not considerably influenced, within the investigated range, by changes in PEG chain lengths and in DSPE-PEG/PA-Chol proportion. The post-insertion of PEG chains reduced in vitro complement activation as well as in vitro macrophage uptake compared to the non-modified STEs. Moreover, longer blood circulation time in mice was established for PEG-modified STEs intravenously injected compared to non-modified STEs. These results establish that post-insertion process of PEG chains to STEs is a promising strategy for developing long-term circulating drug delivery nanocarriers.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100753
Cieślak, Anna; Wauthoz, Nathalie; Nieto Orellana, Alejandro; Lautram, Nolwenn; Béjaud, Jérôme; et al.; Stealth nanocarriers based sterosomes using PEG post-insertion process; Elsevier Science; European Journal Of Pharmaceutics And Biopharmaceutics; 115; 6-2017; 31-38
0939-6411
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100753
identifier_str_mv Cieślak, Anna; Wauthoz, Nathalie; Nieto Orellana, Alejandro; Lautram, Nolwenn; Béjaud, Jérôme; et al.; Stealth nanocarriers based sterosomes using PEG post-insertion process; Elsevier Science; European Journal Of Pharmaceutics And Biopharmaceutics; 115; 6-2017; 31-38
0939-6411
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0939641117301996
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejpb.2017.02.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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