Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide

Autores
Kappaun, Karine; Martinelli, Anne H. S.; Broll, Valquiria; Zambelli, Barbara; Lopes, Fernanda C.; Ligabue-Braun, Rodrigo; Fruttero, Leonardo Luis; Moyetta, Natalia Rita; Bonan, Carla D.; Carlini, Célia Regina R. S.; Ciurli, Stefano
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ureases from different biological sources display non-ureolytic properties that contribute to plant defense, in addition to their classical enzymatic urea hydrolysis. Antifungal and entomotoxic effects were demonstrated for Jaburetox, an intrinsically disordered polypeptide derived from jack bean (Canavalia ensiformis) urease. Here we describe the properties of Soyuretox, a polypeptide derived from soybean (Glycine max) ubiquitous urease. Soyuretox was fungitoxic to Candida albicans, leading to the production of reactive oxygen species. Soyuretox further induced aggregation of Rhodnius prolixus hemocytes, indicating an interference on the insect immune response. No relevant toxicity of Soyuretox to zebrafish larvae was observed. These data suggest the presence of antifungal and entomotoxic portions of the amino acid sequences encompassing both Soyuretox and Jaburetox, despite their small sequence identity. Nuclear Magnetic Resonance (NMR) and circular dichroism (CD) spectroscopic data revealed that Soyuretox, in analogy with Jaburetox, possesses an intrinsic and largely disordered nature. Some folding is observed upon interaction of Soyuretox with sodium dodecyl sulfate (SDS) micelles, taken here as models for membranes. This observation suggests the possibility for this protein to modify its secondary structure upon interaction with the cells of the affected organisms, leading to alterations of membrane integrity. Altogether, Soyuretox can be considered a promising biopesticide for use in plant protection.
Fil: Kappaun, Karine. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Martinelli, Anne H. S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Broll, Valquiria. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Zambelli, Barbara. Universidad de Bologna; Italia
Fil: Lopes, Fernanda C.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Ligabue-Braun, Rodrigo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fruttero, Leonardo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Moyetta, Natalia Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Bonan, Carla D.. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Carlini, Célia Regina R. S.. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Ciurli, Stefano. Universidad de Bologna; Italia
Materia
ANTIFUNGAL ENTOMOTOXIN
CIRCULAR DICHROISM (CD)
INTRINSICALLY DISORDERED PROTEINS
MOLECULAR DYNAMICS (MD)
NUCLEAR MAGNETIC RESONANCE (NMR)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128861
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/128861
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticideKappaun, KarineMartinelli, Anne H. S.Broll, ValquiriaZambelli, BarbaraLopes, Fernanda C.Ligabue-Braun, RodrigoFruttero, Leonardo LuisMoyetta, Natalia RitaBonan, Carla D.Carlini, Célia Regina R. S.Ciurli, StefanoANTIFUNGAL ENTOMOTOXINCIRCULAR DICHROISM (CD)INTRINSICALLY DISORDERED PROTEINSMOLECULAR DYNAMICS (MD)NUCLEAR MAGNETIC RESONANCE (NMR)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ureases from different biological sources display non-ureolytic properties that contribute to plant defense, in addition to their classical enzymatic urea hydrolysis. Antifungal and entomotoxic effects were demonstrated for Jaburetox, an intrinsically disordered polypeptide derived from jack bean (Canavalia ensiformis) urease. Here we describe the properties of Soyuretox, a polypeptide derived from soybean (Glycine max) ubiquitous urease. Soyuretox was fungitoxic to Candida albicans, leading to the production of reactive oxygen species. Soyuretox further induced aggregation of Rhodnius prolixus hemocytes, indicating an interference on the insect immune response. No relevant toxicity of Soyuretox to zebrafish larvae was observed. These data suggest the presence of antifungal and entomotoxic portions of the amino acid sequences encompassing both Soyuretox and Jaburetox, despite their small sequence identity. Nuclear Magnetic Resonance (NMR) and circular dichroism (CD) spectroscopic data revealed that Soyuretox, in analogy with Jaburetox, possesses an intrinsic and largely disordered nature. Some folding is observed upon interaction of Soyuretox with sodium dodecyl sulfate (SDS) micelles, taken here as models for membranes. This observation suggests the possibility for this protein to modify its secondary structure upon interaction with the cells of the affected organisms, leading to alterations of membrane integrity. Altogether, Soyuretox can be considered a promising biopesticide for use in plant protection.Fil: Kappaun, Karine. Pontificia Universidade Católica do Rio Grande do Sul; BrasilFil: Martinelli, Anne H. S.. Universidade Federal do Rio Grande do Sul; BrasilFil: Broll, Valquiria. Universidade Federal do Rio Grande do Sul; BrasilFil: Zambelli, Barbara. Universidad de Bologna; ItaliaFil: Lopes, Fernanda C.. Universidade Federal do Rio Grande do Sul; BrasilFil: Ligabue-Braun, Rodrigo. Universidade Federal do Rio Grande do Sul; BrasilFil: Fruttero, Leonardo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Moyetta, Natalia Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Bonan, Carla D.. Pontificia Universidade Católica do Rio Grande do Sul; BrasilFil: Carlini, Célia Regina R. S.. Pontificia Universidade Católica do Rio Grande do Sul; BrasilFil: Ciurli, Stefano. Universidad de Bologna; ItaliaMDPI AG2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128861Kappaun, Karine; Martinelli, Anne H. S.; Broll, Valquiria; Zambelli, Barbara; Lopes, Fernanda C.; et al.; Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide; MDPI AG; International Journal of Molecular Sciences; 20; 21; 11-20191661-65961422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/20/21/5401info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20215401info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:30Zoai:ri.conicet.gov.ar:11336/128861instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:30.372CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide
title Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide
spellingShingle Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide
Kappaun, Karine
ANTIFUNGAL ENTOMOTOXIN
CIRCULAR DICHROISM (CD)
INTRINSICALLY DISORDERED PROTEINS
MOLECULAR DYNAMICS (MD)
NUCLEAR MAGNETIC RESONANCE (NMR)
title_short Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide
title_full Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide
title_fullStr Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide
title_full_unstemmed Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide
title_sort Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide
dc.creator.none.fl_str_mv Kappaun, Karine
Martinelli, Anne H. S.
Broll, Valquiria
Zambelli, Barbara
Lopes, Fernanda C.
Ligabue-Braun, Rodrigo
Fruttero, Leonardo Luis
Moyetta, Natalia Rita
Bonan, Carla D.
Carlini, Célia Regina R. S.
Ciurli, Stefano
author Kappaun, Karine
author_facet Kappaun, Karine
Martinelli, Anne H. S.
Broll, Valquiria
Zambelli, Barbara
Lopes, Fernanda C.
Ligabue-Braun, Rodrigo
Fruttero, Leonardo Luis
Moyetta, Natalia Rita
Bonan, Carla D.
Carlini, Célia Regina R. S.
Ciurli, Stefano
author_role author
author2 Martinelli, Anne H. S.
Broll, Valquiria
Zambelli, Barbara
Lopes, Fernanda C.
Ligabue-Braun, Rodrigo
Fruttero, Leonardo Luis
Moyetta, Natalia Rita
Bonan, Carla D.
Carlini, Célia Regina R. S.
Ciurli, Stefano
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIFUNGAL ENTOMOTOXIN
CIRCULAR DICHROISM (CD)
INTRINSICALLY DISORDERED PROTEINS
MOLECULAR DYNAMICS (MD)
NUCLEAR MAGNETIC RESONANCE (NMR)
topic ANTIFUNGAL ENTOMOTOXIN
CIRCULAR DICHROISM (CD)
INTRINSICALLY DISORDERED PROTEINS
MOLECULAR DYNAMICS (MD)
NUCLEAR MAGNETIC RESONANCE (NMR)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ureases from different biological sources display non-ureolytic properties that contribute to plant defense, in addition to their classical enzymatic urea hydrolysis. Antifungal and entomotoxic effects were demonstrated for Jaburetox, an intrinsically disordered polypeptide derived from jack bean (Canavalia ensiformis) urease. Here we describe the properties of Soyuretox, a polypeptide derived from soybean (Glycine max) ubiquitous urease. Soyuretox was fungitoxic to Candida albicans, leading to the production of reactive oxygen species. Soyuretox further induced aggregation of Rhodnius prolixus hemocytes, indicating an interference on the insect immune response. No relevant toxicity of Soyuretox to zebrafish larvae was observed. These data suggest the presence of antifungal and entomotoxic portions of the amino acid sequences encompassing both Soyuretox and Jaburetox, despite their small sequence identity. Nuclear Magnetic Resonance (NMR) and circular dichroism (CD) spectroscopic data revealed that Soyuretox, in analogy with Jaburetox, possesses an intrinsic and largely disordered nature. Some folding is observed upon interaction of Soyuretox with sodium dodecyl sulfate (SDS) micelles, taken here as models for membranes. This observation suggests the possibility for this protein to modify its secondary structure upon interaction with the cells of the affected organisms, leading to alterations of membrane integrity. Altogether, Soyuretox can be considered a promising biopesticide for use in plant protection.
Fil: Kappaun, Karine. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Martinelli, Anne H. S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Broll, Valquiria. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Zambelli, Barbara. Universidad de Bologna; Italia
Fil: Lopes, Fernanda C.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Ligabue-Braun, Rodrigo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fruttero, Leonardo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Moyetta, Natalia Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Bonan, Carla D.. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Carlini, Célia Regina R. S.. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Ciurli, Stefano. Universidad de Bologna; Italia
description Ureases from different biological sources display non-ureolytic properties that contribute to plant defense, in addition to their classical enzymatic urea hydrolysis. Antifungal and entomotoxic effects were demonstrated for Jaburetox, an intrinsically disordered polypeptide derived from jack bean (Canavalia ensiformis) urease. Here we describe the properties of Soyuretox, a polypeptide derived from soybean (Glycine max) ubiquitous urease. Soyuretox was fungitoxic to Candida albicans, leading to the production of reactive oxygen species. Soyuretox further induced aggregation of Rhodnius prolixus hemocytes, indicating an interference on the insect immune response. No relevant toxicity of Soyuretox to zebrafish larvae was observed. These data suggest the presence of antifungal and entomotoxic portions of the amino acid sequences encompassing both Soyuretox and Jaburetox, despite their small sequence identity. Nuclear Magnetic Resonance (NMR) and circular dichroism (CD) spectroscopic data revealed that Soyuretox, in analogy with Jaburetox, possesses an intrinsic and largely disordered nature. Some folding is observed upon interaction of Soyuretox with sodium dodecyl sulfate (SDS) micelles, taken here as models for membranes. This observation suggests the possibility for this protein to modify its secondary structure upon interaction with the cells of the affected organisms, leading to alterations of membrane integrity. Altogether, Soyuretox can be considered a promising biopesticide for use in plant protection.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128861
Kappaun, Karine; Martinelli, Anne H. S.; Broll, Valquiria; Zambelli, Barbara; Lopes, Fernanda C.; et al.; Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide; MDPI AG; International Journal of Molecular Sciences; 20; 21; 11-2019
1661-6596
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128861
identifier_str_mv Kappaun, Karine; Martinelli, Anne H. S.; Broll, Valquiria; Zambelli, Barbara; Lopes, Fernanda C.; et al.; Soyuretox, an intrinsically disordered polypeptide derived from soybean (Glycine max) ubiquitous urease with potential use as a biopesticide; MDPI AG; International Journal of Molecular Sciences; 20; 21; 11-2019
1661-6596
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/20/21/5401
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20215401
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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