Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes

Autores
Samelo, Jaime; Mora, Maria Julia; Granero, Gladys Ester; Moreno, Maria João
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A protocol is developed to allow the accurate characterization of partition to lipid bilayers for solutes with low affinity, using isothermal titration calorimetry. The methodology proposed is suitable for studies using complex membranes, such as intact biomembranes or whole cells. In the method developed, the association is characterized at increasing solute concentrations. This allows the characterization of solute partition into unperturbed membranes, as well as effects induced by high solute concentrations. Most druglike molecules are expected to interact with lowto-moderate affinity with relevant cell membranes. This is due to both the need for a relatively high aqueous solubility of the drug and the poor binding properties of the cell membranes. The methodology is applied to characterize the interaction of antibiotic Rifampicin with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and with lipid bilayers representative of bacterial membranes.
Fil: Samelo, Jaime. CQC-Biological Chemistry Group; Portugal
Fil: Mora, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Moreno, Maria João. CQC-Biological Chemistry Group; Portugal
Materia
Partition of Amphiphilic Molecules
Lipid Bilayers
Isothermal Titration Calorimetry
Rifampicin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65828
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/65828
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity SolutesSamelo, JaimeMora, Maria JuliaGranero, Gladys EsterMoreno, Maria JoãoPartition of Amphiphilic MoleculesLipid BilayersIsothermal Titration CalorimetryRifampicinhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2A protocol is developed to allow the accurate characterization of partition to lipid bilayers for solutes with low affinity, using isothermal titration calorimetry. The methodology proposed is suitable for studies using complex membranes, such as intact biomembranes or whole cells. In the method developed, the association is characterized at increasing solute concentrations. This allows the characterization of solute partition into unperturbed membranes, as well as effects induced by high solute concentrations. Most druglike molecules are expected to interact with lowto-moderate affinity with relevant cell membranes. This is due to both the need for a relatively high aqueous solubility of the drug and the poor binding properties of the cell membranes. The methodology is applied to characterize the interaction of antibiotic Rifampicin with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and with lipid bilayers representative of bacterial membranes.Fil: Samelo, Jaime. CQC-Biological Chemistry Group; PortugalFil: Mora, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Moreno, Maria João. CQC-Biological Chemistry Group; PortugalAmerican Chemical Society2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65828Samelo, Jaime; Mora, Maria Julia; Granero, Gladys Ester; Moreno, Maria João; Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes; American Chemical Society; ACS Omega; 2; 10; 2-2017; 6863-68692470-1343CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/pdf/10.1021/acsomega.7b01145info:eu-repo/semantics/altIdentifier/doi/10.1021/acsomega.7b01145info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:21Zoai:ri.conicet.gov.ar:11336/65828instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:21.45CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes
title Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes
spellingShingle Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes
Samelo, Jaime
Partition of Amphiphilic Molecules
Lipid Bilayers
Isothermal Titration Calorimetry
Rifampicin
title_short Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes
title_full Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes
title_fullStr Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes
title_full_unstemmed Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes
title_sort Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes
dc.creator.none.fl_str_mv Samelo, Jaime
Mora, Maria Julia
Granero, Gladys Ester
Moreno, Maria João
author Samelo, Jaime
author_facet Samelo, Jaime
Mora, Maria Julia
Granero, Gladys Ester
Moreno, Maria João
author_role author
author2 Mora, Maria Julia
Granero, Gladys Ester
Moreno, Maria João
author2_role author
author
author
dc.subject.none.fl_str_mv Partition of Amphiphilic Molecules
Lipid Bilayers
Isothermal Titration Calorimetry
Rifampicin
topic Partition of Amphiphilic Molecules
Lipid Bilayers
Isothermal Titration Calorimetry
Rifampicin
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A protocol is developed to allow the accurate characterization of partition to lipid bilayers for solutes with low affinity, using isothermal titration calorimetry. The methodology proposed is suitable for studies using complex membranes, such as intact biomembranes or whole cells. In the method developed, the association is characterized at increasing solute concentrations. This allows the characterization of solute partition into unperturbed membranes, as well as effects induced by high solute concentrations. Most druglike molecules are expected to interact with lowto-moderate affinity with relevant cell membranes. This is due to both the need for a relatively high aqueous solubility of the drug and the poor binding properties of the cell membranes. The methodology is applied to characterize the interaction of antibiotic Rifampicin with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and with lipid bilayers representative of bacterial membranes.
Fil: Samelo, Jaime. CQC-Biological Chemistry Group; Portugal
Fil: Mora, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Moreno, Maria João. CQC-Biological Chemistry Group; Portugal
description A protocol is developed to allow the accurate characterization of partition to lipid bilayers for solutes with low affinity, using isothermal titration calorimetry. The methodology proposed is suitable for studies using complex membranes, such as intact biomembranes or whole cells. In the method developed, the association is characterized at increasing solute concentrations. This allows the characterization of solute partition into unperturbed membranes, as well as effects induced by high solute concentrations. Most druglike molecules are expected to interact with lowto-moderate affinity with relevant cell membranes. This is due to both the need for a relatively high aqueous solubility of the drug and the poor binding properties of the cell membranes. The methodology is applied to characterize the interaction of antibiotic Rifampicin with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and with lipid bilayers representative of bacterial membranes.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65828
Samelo, Jaime; Mora, Maria Julia; Granero, Gladys Ester; Moreno, Maria João; Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes; American Chemical Society; ACS Omega; 2; 10; 2-2017; 6863-6869
2470-1343
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65828
identifier_str_mv Samelo, Jaime; Mora, Maria Julia; Granero, Gladys Ester; Moreno, Maria João; Partition of Amphiphilic Molecules to Lipid Bilayers by ITC: LowAffinity Solutes; American Chemical Society; ACS Omega; 2; 10; 2-2017; 6863-6869
2470-1343
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/pdf/10.1021/acsomega.7b01145
info:eu-repo/semantics/altIdentifier/doi/10.1021/acsomega.7b01145
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268596802158592
score 13.13397

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