Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function

Autores
Susperreguy, Sebastian; Yamashita, Megumi; Choi, Chan-il; Liao, Yanhong; Burch, Lauranell H.; Blankenship, Terry L.; Hayes, Erika; Sliwa, Thomas; Zhang, Yingpei; Grenet, Dagoberto; Walker, Mitzie; Plummer, Nicholas W.; Abramowitz, Joel; Kinet, Jean Pierre; Formoso, Karina; Johnson, Brandon E.; Fleig, Andrea; Hazlehurst, Lori; Penner, Reinhold; Freichel, Marc; Flockerzi, Veit; Prakriya, Murali; Birnbaumer, Lutz
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Using genetically engineered mice andcell lines derived from genetically engineered mice we show that depletion ofER delimited Ca2+ stores activate heteromeric Ca2+ entry (SOCE)channels formed obligatorily, but not exclusively by Orai1 molecules.Comparison of Orai dependent Ca2+ entries revealed Orai1 to bedominant when compared to Orai2 and Orai3. Unexpectedly, we found that storedepletion activated Ca2+ entry does not depend obligatorily on functionallyintact TRPC molecules, as SOCE monitored with the Fura2 Ca2+reporter dye is unaffected in cells in which all seven TRPC coding genes havebeen structurally and functionally inactivated. Unexpectedly as well, we foundthat TRPC-independent Gq-coupled receptor operated Ca2+ entry (ROCE)also depends on Orai1. Biophysical measurements of Ca2+ releaseactivated Ca2+ currents (Icrac) are likewise unaffected by ablationof all seven TRPC genes. We refer to mice and cells carrying the seven-fold disruptionof TRPC genes as TRPC heptaKO mice and cells. TRPC heptaKO mice are fertile allowingthe creation of a new homozygous inbred strain.
Fil: Susperreguy, Sebastian. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Yamashita, Megumi. Northwestern University; Estados Unidos
Fil: Choi, Chan-il. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Liao, Yanhong. National Institute of Environmental Health Sciences; Estados Unidos. Huazhong University of Science and Technology; China
Fil: Burch, Lauranell H.. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Blankenship, Terry L.. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Hayes, Erika. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Sliwa, Thomas. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Zhang, Yingpei. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Grenet, Dagoberto. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Walker, Mitzie. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Plummer, Nicholas W.. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Abramowitz, Joel. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Kinet, Jean Pierre. Israel Deaconess Medical Center; Israel. Harvard Medical School; Estados Unidos
Fil: Formoso, Karina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Johnson, Brandon E.. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados Unidos
Fil: Fleig, Andrea. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados Unidos
Fil: Hazlehurst, Lori. West Virginia University; Estados Unidos
Fil: Penner, Reinhold. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados Unidos
Fil: Freichel, Marc. Universitat Saarland; Alemania. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Flockerzi, Veit. Universitat Saarland; Alemania
Fil: Prakriya, Murali. Northwestern University; Estados Unidos
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. National Institute of Environmental Health Sciences; Estados Unidos
Materia
TRPC
SOCE
ORAI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266092
Acceder
id CONICETDig_638e2533c9e7e073fe31f19d3cb2c459
oai_identifier_str oai:ri.conicet.gov.ar:11336/266092
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel functionSusperreguy, SebastianYamashita, MegumiChoi, Chan-ilLiao, YanhongBurch, Lauranell H.Blankenship, Terry L.Hayes, ErikaSliwa, ThomasZhang, YingpeiGrenet, DagobertoWalker, MitziePlummer, Nicholas W.Abramowitz, JoelKinet, Jean PierreFormoso, KarinaJohnson, Brandon E.Fleig, AndreaHazlehurst, LoriPenner, ReinholdFreichel, MarcFlockerzi, VeitPrakriya, MuraliBirnbaumer, LutzTRPCSOCEORAIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Using genetically engineered mice andcell lines derived from genetically engineered mice we show that depletion ofER delimited Ca2+ stores activate heteromeric Ca2+ entry (SOCE)channels formed obligatorily, but not exclusively by Orai1 molecules.Comparison of Orai dependent Ca2+ entries revealed Orai1 to bedominant when compared to Orai2 and Orai3. Unexpectedly, we found that storedepletion activated Ca2+ entry does not depend obligatorily on functionallyintact TRPC molecules, as SOCE monitored with the Fura2 Ca2+reporter dye is unaffected in cells in which all seven TRPC coding genes havebeen structurally and functionally inactivated. Unexpectedly as well, we foundthat TRPC-independent Gq-coupled receptor operated Ca2+ entry (ROCE)also depends on Orai1. Biophysical measurements of Ca2+ releaseactivated Ca2+ currents (Icrac) are likewise unaffected by ablationof all seven TRPC genes. We refer to mice and cells carrying the seven-fold disruptionof TRPC genes as TRPC heptaKO mice and cells. TRPC heptaKO mice are fertile allowingthe creation of a new homozygous inbred strain.Fil: Susperreguy, Sebastian. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Yamashita, Megumi. Northwestern University; Estados UnidosFil: Choi, Chan-il. National Institute of Environmental Health Sciences; Estados UnidosFil: Liao, Yanhong. National Institute of Environmental Health Sciences; Estados Unidos. Huazhong University of Science and Technology; ChinaFil: Burch, Lauranell H.. National Institute of Environmental Health Sciences; Estados UnidosFil: Blankenship, Terry L.. National Institute of Environmental Health Sciences; Estados UnidosFil: Hayes, Erika. National Institute of Environmental Health Sciences; Estados UnidosFil: Sliwa, Thomas. National Institute of Environmental Health Sciences; Estados UnidosFil: Zhang, Yingpei. National Institute of Environmental Health Sciences; Estados UnidosFil: Grenet, Dagoberto. National Institute of Environmental Health Sciences; Estados UnidosFil: Walker, Mitzie. National Institute of Environmental Health Sciences; Estados UnidosFil: Plummer, Nicholas W.. National Institute of Environmental Health Sciences; Estados UnidosFil: Abramowitz, Joel. National Institute of Environmental Health Sciences; Estados UnidosFil: Kinet, Jean Pierre. Israel Deaconess Medical Center; Israel. Harvard Medical School; Estados UnidosFil: Formoso, Karina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Johnson, Brandon E.. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados UnidosFil: Fleig, Andrea. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados UnidosFil: Hazlehurst, Lori. West Virginia University; Estados UnidosFil: Penner, Reinhold. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados UnidosFil: Freichel, Marc. Universitat Saarland; Alemania. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Flockerzi, Veit. Universitat Saarland; AlemaniaFil: Prakriya, Murali. Northwestern University; Estados UnidosFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. National Institute of Environmental Health Sciences; Estados UnidosNational Academy of Sciences2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266092Susperreguy, Sebastian; Yamashita, Megumi; Choi, Chan-il; Liao, Yanhong; Burch, Lauranell H.; et al.; Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 121; 49; 11-2024; 1-120027-8424CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pnas.org/doi/10.1073/pnas.2411389121info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.2411389121info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:00Zoai:ri.conicet.gov.ar:11336/266092instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:00.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function
title Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function
spellingShingle Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function
Susperreguy, Sebastian
TRPC
SOCE
ORAI
title_short Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function
title_full Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function
title_fullStr Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function
title_full_unstemmed Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function
title_sort Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function
dc.creator.none.fl_str_mv Susperreguy, Sebastian
Yamashita, Megumi
Choi, Chan-il
Liao, Yanhong
Burch, Lauranell H.
Blankenship, Terry L.
Hayes, Erika
Sliwa, Thomas
Zhang, Yingpei
Grenet, Dagoberto
Walker, Mitzie
Plummer, Nicholas W.
Abramowitz, Joel
Kinet, Jean Pierre
Formoso, Karina
Johnson, Brandon E.
Fleig, Andrea
Hazlehurst, Lori
Penner, Reinhold
Freichel, Marc
Flockerzi, Veit
Prakriya, Murali
Birnbaumer, Lutz
author Susperreguy, Sebastian
author_facet Susperreguy, Sebastian
Yamashita, Megumi
Choi, Chan-il
Liao, Yanhong
Burch, Lauranell H.
Blankenship, Terry L.
Hayes, Erika
Sliwa, Thomas
Zhang, Yingpei
Grenet, Dagoberto
Walker, Mitzie
Plummer, Nicholas W.
Abramowitz, Joel
Kinet, Jean Pierre
Formoso, Karina
Johnson, Brandon E.
Fleig, Andrea
Hazlehurst, Lori
Penner, Reinhold
Freichel, Marc
Flockerzi, Veit
Prakriya, Murali
Birnbaumer, Lutz
author_role author
author2 Yamashita, Megumi
Choi, Chan-il
Liao, Yanhong
Burch, Lauranell H.
Blankenship, Terry L.
Hayes, Erika
Sliwa, Thomas
Zhang, Yingpei
Grenet, Dagoberto
Walker, Mitzie
Plummer, Nicholas W.
Abramowitz, Joel
Kinet, Jean Pierre
Formoso, Karina
Johnson, Brandon E.
Fleig, Andrea
Hazlehurst, Lori
Penner, Reinhold
Freichel, Marc
Flockerzi, Veit
Prakriya, Murali
Birnbaumer, Lutz
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRPC
SOCE
ORAI
topic TRPC
SOCE
ORAI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Using genetically engineered mice andcell lines derived from genetically engineered mice we show that depletion ofER delimited Ca2+ stores activate heteromeric Ca2+ entry (SOCE)channels formed obligatorily, but not exclusively by Orai1 molecules.Comparison of Orai dependent Ca2+ entries revealed Orai1 to bedominant when compared to Orai2 and Orai3. Unexpectedly, we found that storedepletion activated Ca2+ entry does not depend obligatorily on functionallyintact TRPC molecules, as SOCE monitored with the Fura2 Ca2+reporter dye is unaffected in cells in which all seven TRPC coding genes havebeen structurally and functionally inactivated. Unexpectedly as well, we foundthat TRPC-independent Gq-coupled receptor operated Ca2+ entry (ROCE)also depends on Orai1. Biophysical measurements of Ca2+ releaseactivated Ca2+ currents (Icrac) are likewise unaffected by ablationof all seven TRPC genes. We refer to mice and cells carrying the seven-fold disruptionof TRPC genes as TRPC heptaKO mice and cells. TRPC heptaKO mice are fertile allowingthe creation of a new homozygous inbred strain.
Fil: Susperreguy, Sebastian. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Yamashita, Megumi. Northwestern University; Estados Unidos
Fil: Choi, Chan-il. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Liao, Yanhong. National Institute of Environmental Health Sciences; Estados Unidos. Huazhong University of Science and Technology; China
Fil: Burch, Lauranell H.. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Blankenship, Terry L.. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Hayes, Erika. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Sliwa, Thomas. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Zhang, Yingpei. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Grenet, Dagoberto. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Walker, Mitzie. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Plummer, Nicholas W.. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Abramowitz, Joel. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Kinet, Jean Pierre. Israel Deaconess Medical Center; Israel. Harvard Medical School; Estados Unidos
Fil: Formoso, Karina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Johnson, Brandon E.. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados Unidos
Fil: Fleig, Andrea. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados Unidos
Fil: Hazlehurst, Lori. West Virginia University; Estados Unidos
Fil: Penner, Reinhold. University of Hawaii at Manoa; Estados Unidos. The Queen’s Medical Center; Estados Unidos
Fil: Freichel, Marc. Universitat Saarland; Alemania. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Flockerzi, Veit. Universitat Saarland; Alemania
Fil: Prakriya, Murali. Northwestern University; Estados Unidos
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. National Institute of Environmental Health Sciences; Estados Unidos
description Using genetically engineered mice andcell lines derived from genetically engineered mice we show that depletion ofER delimited Ca2+ stores activate heteromeric Ca2+ entry (SOCE)channels formed obligatorily, but not exclusively by Orai1 molecules.Comparison of Orai dependent Ca2+ entries revealed Orai1 to bedominant when compared to Orai2 and Orai3. Unexpectedly, we found that storedepletion activated Ca2+ entry does not depend obligatorily on functionallyintact TRPC molecules, as SOCE monitored with the Fura2 Ca2+reporter dye is unaffected in cells in which all seven TRPC coding genes havebeen structurally and functionally inactivated. Unexpectedly as well, we foundthat TRPC-independent Gq-coupled receptor operated Ca2+ entry (ROCE)also depends on Orai1. Biophysical measurements of Ca2+ releaseactivated Ca2+ currents (Icrac) are likewise unaffected by ablationof all seven TRPC genes. We refer to mice and cells carrying the seven-fold disruptionof TRPC genes as TRPC heptaKO mice and cells. TRPC heptaKO mice are fertile allowingthe creation of a new homozygous inbred strain.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266092
Susperreguy, Sebastian; Yamashita, Megumi; Choi, Chan-il; Liao, Yanhong; Burch, Lauranell H.; et al.; Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 121; 49; 11-2024; 1-12
0027-8424
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266092
identifier_str_mv Susperreguy, Sebastian; Yamashita, Megumi; Choi, Chan-il; Liao, Yanhong; Burch, Lauranell H.; et al.; Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 121; 49; 11-2024; 1-12
0027-8424
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pnas.org/doi/10.1073/pnas.2411389121
info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.2411389121
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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