In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases

Autores
Castro Cabrera, Inelvis; Vives Hernández, Karel; Bruno, Mariela Anahí; Obregón, Walter David; Hernández de la Torre, Martha
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
P. chilensis is an endemic species of Chile belonging to the Bromeliaceae family, known for its morphological plasticity and ecological importance. In recent years, greater attention has been paid to its conservation due to the indiscriminate use of its populations for food and medicinal purposes. In vitro culture is a tool for the propagation and conservation of endemic species, as well as for obtaining bioactive plant molecules. In Chile, P. chilensis is the most representative within the Puya genus. This study was focused to establish an in vitro propagation protocol of P. chilensis using culture systems that allow the production of proteolytic enzymes. The combined effect of plants cuts and the application of 0.5 µmol. L-1 of BAP and GA3 favored the in vitro multiplication of P. chilensis. Culture in liquid medium induced greater morphological development and early differentiation of anatomical structures in the leaves of P. chilensis in vitro. The TIS provided photoautotrophic conditions during in vitro cultivation and guaranteed 100% plant survival upon transfer to the greenhouse. The management of cultivation conditions and the efficient use of TIS allowed the generation of P. chilensis plants with an optimal degree of development for obtaining proteolytic extracts. The main enzymes present in the extracts of P. chilensis plants grown in vitro belong to the cysteine type. This study proposes for the first time an optimized protocol for the propagation and conservation of P. chilensis, enhancing its uses as a source of biologically active molecules for the biotechnology and pharmaceutical industries.
Fil: Castro Cabrera, Inelvis. Universidad de Concepción; Chile
Fil: Vives Hernández, Karel. Universidad de Concepción; Chile
Fil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Obregón, Walter David. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Hernández de la Torre, Martha. Universidad de Concepción; Chile
Materia
PUYA CHILENSIS
IN VITRO CULTURE
PROTEASES
TEMPORARY IMMERSION SYSTEMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268726
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteasesCastro Cabrera, InelvisVives Hernández, KarelBruno, Mariela AnahíObregón, Walter DavidHernández de la Torre, MarthaPUYA CHILENSISIN VITRO CULTUREPROTEASESTEMPORARY IMMERSION SYSTEMShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1P. chilensis is an endemic species of Chile belonging to the Bromeliaceae family, known for its morphological plasticity and ecological importance. In recent years, greater attention has been paid to its conservation due to the indiscriminate use of its populations for food and medicinal purposes. In vitro culture is a tool for the propagation and conservation of endemic species, as well as for obtaining bioactive plant molecules. In Chile, P. chilensis is the most representative within the Puya genus. This study was focused to establish an in vitro propagation protocol of P. chilensis using culture systems that allow the production of proteolytic enzymes. The combined effect of plants cuts and the application of 0.5 µmol. L-1 of BAP and GA3 favored the in vitro multiplication of P. chilensis. Culture in liquid medium induced greater morphological development and early differentiation of anatomical structures in the leaves of P. chilensis in vitro. The TIS provided photoautotrophic conditions during in vitro cultivation and guaranteed 100% plant survival upon transfer to the greenhouse. The management of cultivation conditions and the efficient use of TIS allowed the generation of P. chilensis plants with an optimal degree of development for obtaining proteolytic extracts. The main enzymes present in the extracts of P. chilensis plants grown in vitro belong to the cysteine type. This study proposes for the first time an optimized protocol for the propagation and conservation of P. chilensis, enhancing its uses as a source of biologically active molecules for the biotechnology and pharmaceutical industries.Fil: Castro Cabrera, Inelvis. Universidad de Concepción; ChileFil: Vives Hernández, Karel. Universidad de Concepción; ChileFil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Obregón, Walter David. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Hernández de la Torre, Martha. Universidad de Concepción; ChileSpringer2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268726Castro Cabrera, Inelvis; Vives Hernández, Karel; Bruno, Mariela Anahí; Obregón, Walter David; Hernández de la Torre, Martha; In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases; Springer; Plant Cell; 6-2024; 1-151573-5044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4774144info:eu-repo/semantics/altIdentifier/doi/10.2139/ssrn.4774144info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:50Zoai:ri.conicet.gov.ar:11336/268726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:50.475CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
title In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
spellingShingle In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
Castro Cabrera, Inelvis
PUYA CHILENSIS
IN VITRO CULTURE
PROTEASES
TEMPORARY IMMERSION SYSTEMS
title_short In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
title_full In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
title_fullStr In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
title_full_unstemmed In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
title_sort In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases
dc.creator.none.fl_str_mv Castro Cabrera, Inelvis
Vives Hernández, Karel
Bruno, Mariela Anahí
Obregón, Walter David
Hernández de la Torre, Martha
author Castro Cabrera, Inelvis
author_facet Castro Cabrera, Inelvis
Vives Hernández, Karel
Bruno, Mariela Anahí
Obregón, Walter David
Hernández de la Torre, Martha
author_role author
author2 Vives Hernández, Karel
Bruno, Mariela Anahí
Obregón, Walter David
Hernández de la Torre, Martha
author2_role author
author
author
author
dc.subject.none.fl_str_mv PUYA CHILENSIS
IN VITRO CULTURE
PROTEASES
TEMPORARY IMMERSION SYSTEMS
topic PUYA CHILENSIS
IN VITRO CULTURE
PROTEASES
TEMPORARY IMMERSION SYSTEMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv P. chilensis is an endemic species of Chile belonging to the Bromeliaceae family, known for its morphological plasticity and ecological importance. In recent years, greater attention has been paid to its conservation due to the indiscriminate use of its populations for food and medicinal purposes. In vitro culture is a tool for the propagation and conservation of endemic species, as well as for obtaining bioactive plant molecules. In Chile, P. chilensis is the most representative within the Puya genus. This study was focused to establish an in vitro propagation protocol of P. chilensis using culture systems that allow the production of proteolytic enzymes. The combined effect of plants cuts and the application of 0.5 µmol. L-1 of BAP and GA3 favored the in vitro multiplication of P. chilensis. Culture in liquid medium induced greater morphological development and early differentiation of anatomical structures in the leaves of P. chilensis in vitro. The TIS provided photoautotrophic conditions during in vitro cultivation and guaranteed 100% plant survival upon transfer to the greenhouse. The management of cultivation conditions and the efficient use of TIS allowed the generation of P. chilensis plants with an optimal degree of development for obtaining proteolytic extracts. The main enzymes present in the extracts of P. chilensis plants grown in vitro belong to the cysteine type. This study proposes for the first time an optimized protocol for the propagation and conservation of P. chilensis, enhancing its uses as a source of biologically active molecules for the biotechnology and pharmaceutical industries.
Fil: Castro Cabrera, Inelvis. Universidad de Concepción; Chile
Fil: Vives Hernández, Karel. Universidad de Concepción; Chile
Fil: Bruno, Mariela Anahí. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Obregón, Walter David. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Hernández de la Torre, Martha. Universidad de Concepción; Chile
description P. chilensis is an endemic species of Chile belonging to the Bromeliaceae family, known for its morphological plasticity and ecological importance. In recent years, greater attention has been paid to its conservation due to the indiscriminate use of its populations for food and medicinal purposes. In vitro culture is a tool for the propagation and conservation of endemic species, as well as for obtaining bioactive plant molecules. In Chile, P. chilensis is the most representative within the Puya genus. This study was focused to establish an in vitro propagation protocol of P. chilensis using culture systems that allow the production of proteolytic enzymes. The combined effect of plants cuts and the application of 0.5 µmol. L-1 of BAP and GA3 favored the in vitro multiplication of P. chilensis. Culture in liquid medium induced greater morphological development and early differentiation of anatomical structures in the leaves of P. chilensis in vitro. The TIS provided photoautotrophic conditions during in vitro cultivation and guaranteed 100% plant survival upon transfer to the greenhouse. The management of cultivation conditions and the efficient use of TIS allowed the generation of P. chilensis plants with an optimal degree of development for obtaining proteolytic extracts. The main enzymes present in the extracts of P. chilensis plants grown in vitro belong to the cysteine type. This study proposes for the first time an optimized protocol for the propagation and conservation of P. chilensis, enhancing its uses as a source of biologically active molecules for the biotechnology and pharmaceutical industries.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268726
Castro Cabrera, Inelvis; Vives Hernández, Karel; Bruno, Mariela Anahí; Obregón, Walter David; Hernández de la Torre, Martha; In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases; Springer; Plant Cell; 6-2024; 1-15
1573-5044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268726
identifier_str_mv Castro Cabrera, Inelvis; Vives Hernández, Karel; Bruno, Mariela Anahí; Obregón, Walter David; Hernández de la Torre, Martha; In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases; Springer; Plant Cell; 6-2024; 1-15
1573-5044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4774144
info:eu-repo/semantics/altIdentifier/doi/10.2139/ssrn.4774144
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.221938

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