Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells

Autores
Shi, Jian; Miralles, Francesc; Birnbaumer, Lutz; Large, William A.; Albert, Anthony P.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Depletion of sarcoplasmic reticulum (SR) Ca2+ stores activates store-operated channels (SOCs) composed of canonical transient receptor potential (TRPC) 1 proteins in vascular smooth muscle cells (VSMCs), which contribute to important cellular functions. We have previously shown that PKC is obligatory for activation of TRPC1 SOCs in VSMCs, and the present study investigates if the classic phosphoinositol signaling pathway involving Gαq-mediated PLC activity is responsible for driving PKCdependent channel gating. The G-protein inhibitor GDPb- S, anti-Gαq antibodies, thePLCinhibitorU73122, and the PKCinhibitorGF109203Xall inhibited activation ofTRPC1 SOCs, and U73122 and GF109203X also reduced storeoperated PKC-dependent phosphorylation of TRPC1 proteins. Three distinct SR Ca2+ store-depleting agents, 1,2-bis(2-aminophenoxy)ethane-N,N,N9,N9-tetraacetic acid acetoxymethyl ester, cyclopiazonic acid, and N,N,N9,N9- tetrakis(2-pyridylmethyl)ethane-1,2-diamineed,inducedtranslocations of the fluorescent biosensor GFP-PLCd1-PH from the cell membrane to the cytosol, which were inhibited by U73122. Knockdown of PLCβ1 with small hairpin RNA reduced both store-operated PLC activity and stimulation of TRPC1 SOCs. Immunoprecipitation studies and proximity ligation assays revealed that store depletion induced interactions betweenTRPC1andGαq, andTRPC1and PLCβ1. Wepropose a novel activation mechanismforTRPC1SOCs in VSMCs, in which store depletion induces formation of TRPC1-Gαq-PLCβ1 complexes that lead to PKC stimulation and channel gating.
Fil: Shi, Jian. University of London; Reino Unido
Fil: Miralles, Francesc. University of London; Reino Unido
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park; Estados Unidos
Fil: Large, William A.. University of London; Reino Unido
Fil: Albert, Anthony P.. University of London; Reino Unido
Materia
CA2+ SIGNALING
ELECTROPHYSIOLOGY
PHOSPHOINOSITOL SIGNALING
PLC ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48670
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48670
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cellsShi, JianMiralles, FrancescBirnbaumer, LutzLarge, William A.Albert, Anthony P.CA2+ SIGNALINGELECTROPHYSIOLOGYPHOSPHOINOSITOL SIGNALINGPLC ACTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Depletion of sarcoplasmic reticulum (SR) Ca2+ stores activates store-operated channels (SOCs) composed of canonical transient receptor potential (TRPC) 1 proteins in vascular smooth muscle cells (VSMCs), which contribute to important cellular functions. We have previously shown that PKC is obligatory for activation of TRPC1 SOCs in VSMCs, and the present study investigates if the classic phosphoinositol signaling pathway involving Gαq-mediated PLC activity is responsible for driving PKCdependent channel gating. The G-protein inhibitor GDPb- S, anti-Gαq antibodies, thePLCinhibitorU73122, and the PKCinhibitorGF109203Xall inhibited activation ofTRPC1 SOCs, and U73122 and GF109203X also reduced storeoperated PKC-dependent phosphorylation of TRPC1 proteins. Three distinct SR Ca2+ store-depleting agents, 1,2-bis(2-aminophenoxy)ethane-N,N,N9,N9-tetraacetic acid acetoxymethyl ester, cyclopiazonic acid, and N,N,N9,N9- tetrakis(2-pyridylmethyl)ethane-1,2-diamineed,inducedtranslocations of the fluorescent biosensor GFP-PLCd1-PH from the cell membrane to the cytosol, which were inhibited by U73122. Knockdown of PLCβ1 with small hairpin RNA reduced both store-operated PLC activity and stimulation of TRPC1 SOCs. Immunoprecipitation studies and proximity ligation assays revealed that store depletion induced interactions betweenTRPC1andGαq, andTRPC1and PLCβ1. Wepropose a novel activation mechanismforTRPC1SOCs in VSMCs, in which store depletion induces formation of TRPC1-Gαq-PLCβ1 complexes that lead to PKC stimulation and channel gating.Fil: Shi, Jian. University of London; Reino UnidoFil: Miralles, Francesc. University of London; Reino UnidoFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park; Estados UnidosFil: Large, William A.. University of London; Reino UnidoFil: Albert, Anthony P.. University of London; Reino UnidoFederation of American Societies for Experimental Biology2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48670Shi, Jian; Miralles, Francesc; Birnbaumer, Lutz; Large, William A.; Albert, Anthony P.; Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells; Federation of American Societies for Experimental Biology; FASEB Journal; 30; 2; 2-2016; 702-7150892-6638CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1096/fj.15-280271info:eu-repo/semantics/altIdentifier/url/https://www.fasebj.org/doi/10.1096/fj.15-280271info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:09Zoai:ri.conicet.gov.ar:11336/48670instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:09.428CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells
title Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells
spellingShingle Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells
Shi, Jian
CA2+ SIGNALING
ELECTROPHYSIOLOGY
PHOSPHOINOSITOL SIGNALING
PLC ACTIVITY
title_short Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells
title_full Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells
title_fullStr Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells
title_full_unstemmed Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells
title_sort Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells
dc.creator.none.fl_str_mv Shi, Jian
Miralles, Francesc
Birnbaumer, Lutz
Large, William A.
Albert, Anthony P.
author Shi, Jian
author_facet Shi, Jian
Miralles, Francesc
Birnbaumer, Lutz
Large, William A.
Albert, Anthony P.
author_role author
author2 Miralles, Francesc
Birnbaumer, Lutz
Large, William A.
Albert, Anthony P.
author2_role author
author
author
author
dc.subject.none.fl_str_mv CA2+ SIGNALING
ELECTROPHYSIOLOGY
PHOSPHOINOSITOL SIGNALING
PLC ACTIVITY
topic CA2+ SIGNALING
ELECTROPHYSIOLOGY
PHOSPHOINOSITOL SIGNALING
PLC ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Depletion of sarcoplasmic reticulum (SR) Ca2+ stores activates store-operated channels (SOCs) composed of canonical transient receptor potential (TRPC) 1 proteins in vascular smooth muscle cells (VSMCs), which contribute to important cellular functions. We have previously shown that PKC is obligatory for activation of TRPC1 SOCs in VSMCs, and the present study investigates if the classic phosphoinositol signaling pathway involving Gαq-mediated PLC activity is responsible for driving PKCdependent channel gating. The G-protein inhibitor GDPb- S, anti-Gαq antibodies, thePLCinhibitorU73122, and the PKCinhibitorGF109203Xall inhibited activation ofTRPC1 SOCs, and U73122 and GF109203X also reduced storeoperated PKC-dependent phosphorylation of TRPC1 proteins. Three distinct SR Ca2+ store-depleting agents, 1,2-bis(2-aminophenoxy)ethane-N,N,N9,N9-tetraacetic acid acetoxymethyl ester, cyclopiazonic acid, and N,N,N9,N9- tetrakis(2-pyridylmethyl)ethane-1,2-diamineed,inducedtranslocations of the fluorescent biosensor GFP-PLCd1-PH from the cell membrane to the cytosol, which were inhibited by U73122. Knockdown of PLCβ1 with small hairpin RNA reduced both store-operated PLC activity and stimulation of TRPC1 SOCs. Immunoprecipitation studies and proximity ligation assays revealed that store depletion induced interactions betweenTRPC1andGαq, andTRPC1and PLCβ1. Wepropose a novel activation mechanismforTRPC1SOCs in VSMCs, in which store depletion induces formation of TRPC1-Gαq-PLCβ1 complexes that lead to PKC stimulation and channel gating.
Fil: Shi, Jian. University of London; Reino Unido
Fil: Miralles, Francesc. University of London; Reino Unido
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park; Estados Unidos
Fil: Large, William A.. University of London; Reino Unido
Fil: Albert, Anthony P.. University of London; Reino Unido
description Depletion of sarcoplasmic reticulum (SR) Ca2+ stores activates store-operated channels (SOCs) composed of canonical transient receptor potential (TRPC) 1 proteins in vascular smooth muscle cells (VSMCs), which contribute to important cellular functions. We have previously shown that PKC is obligatory for activation of TRPC1 SOCs in VSMCs, and the present study investigates if the classic phosphoinositol signaling pathway involving Gαq-mediated PLC activity is responsible for driving PKCdependent channel gating. The G-protein inhibitor GDPb- S, anti-Gαq antibodies, thePLCinhibitorU73122, and the PKCinhibitorGF109203Xall inhibited activation ofTRPC1 SOCs, and U73122 and GF109203X also reduced storeoperated PKC-dependent phosphorylation of TRPC1 proteins. Three distinct SR Ca2+ store-depleting agents, 1,2-bis(2-aminophenoxy)ethane-N,N,N9,N9-tetraacetic acid acetoxymethyl ester, cyclopiazonic acid, and N,N,N9,N9- tetrakis(2-pyridylmethyl)ethane-1,2-diamineed,inducedtranslocations of the fluorescent biosensor GFP-PLCd1-PH from the cell membrane to the cytosol, which were inhibited by U73122. Knockdown of PLCβ1 with small hairpin RNA reduced both store-operated PLC activity and stimulation of TRPC1 SOCs. Immunoprecipitation studies and proximity ligation assays revealed that store depletion induced interactions betweenTRPC1andGαq, andTRPC1and PLCβ1. Wepropose a novel activation mechanismforTRPC1SOCs in VSMCs, in which store depletion induces formation of TRPC1-Gαq-PLCβ1 complexes that lead to PKC stimulation and channel gating.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48670
Shi, Jian; Miralles, Francesc; Birnbaumer, Lutz; Large, William A.; Albert, Anthony P.; Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells; Federation of American Societies for Experimental Biology; FASEB Journal; 30; 2; 2-2016; 702-715
0892-6638
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48670
identifier_str_mv Shi, Jian; Miralles, Francesc; Birnbaumer, Lutz; Large, William A.; Albert, Anthony P.; Store depletion induces Gαq-mediated PLCβ1 activity to stimulate TRPC1 channels in vascular smooth muscle cells; Federation of American Societies for Experimental Biology; FASEB Journal; 30; 2; 2-2016; 702-715
0892-6638
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1096/fj.15-280271
info:eu-repo/semantics/altIdentifier/url/https://www.fasebj.org/doi/10.1096/fj.15-280271
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268956390326272
score 13.13397

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