Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy
- Autores
- Gamarra Luques, Carlos Diego; Goyeneche, Alicia A.; Hapon, María Belén; Telleria, Carlos Marcelo
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Advanced ovarian cancer is treated with cytoreductive surgery and combination platinum- and taxane-based chemotherapy. Although most patients have acute clinical response to this strategy, the disease ultimately recurs. In this work we questioned whether the synthetic steroid mifepristone, which as monotherapy inhibits the growth of ovarian cancer cells, is capable of preventing repopulation of ovarian cancer cells if given after a round of lethal cisplatin-paclitaxel combination treatment.Methods: We established an in vitro approach wherein ovarian cancer cells with various sensitivities to cisplatin or paclitaxel were exposed to a round of lethal doses of cisplatin for 1 h plus paclitaxel for 3 h. Thereafter, cells were maintained in media with or without mifepristone, and short- and long-term cytotoxicity was assessed.Results: Four days after treatment the lethality of cisplatin-paclitaxel was evidenced by reduced number of cells, increased hypodiploid DNA content, morphological features of apoptosis, DNA fragmentation, and cleavage of caspase-3, and of its downstream substrate PARP. Short-term presence of mifepristone either enhanced or did not modify such acute lethality. Seven days after receiving cisplatin-paclitaxel, cultures showed signs of relapse with escaping colonies that repopulated the plate in a time-dependent manner. Conversely, cultures exposed to cisplatin-paclitaxel followed by mifepristone not only did not display signs of repopulation following initial chemotherapy, but they also had their clonogenic capacity drastically reduced when compared to cells repopulating after cisplatin-paclitaxel.Conclusions: Cytostatic concentrations of mifepristone after exposure to lethal doses of cisplatin and paclitaxel in combination blocks repopulation of remnant cells surviving and escaping the cytotoxic drugs.
Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. University Of South Dakota. Sanford School Of Medicine; Estados Unidos
Fil: Goyeneche, Alicia A.. University Of South Dakota. Sanford School Of Medicine; Estados Unidos
Fil: Hapon, María Belén. University Of South Dakota. Sanford School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Telleria, Carlos Marcelo. University Of South Dakota. Sanford School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Mifepristone
cancer de ovario
apoptosis
ciclo celular - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/81171
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CONICET Digital (CONICET) |
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Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapyGamarra Luques, Carlos DiegoGoyeneche, Alicia A.Hapon, María BelénTelleria, Carlos MarceloMifepristonecancer de ovarioapoptosisciclo celularhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Advanced ovarian cancer is treated with cytoreductive surgery and combination platinum- and taxane-based chemotherapy. Although most patients have acute clinical response to this strategy, the disease ultimately recurs. In this work we questioned whether the synthetic steroid mifepristone, which as monotherapy inhibits the growth of ovarian cancer cells, is capable of preventing repopulation of ovarian cancer cells if given after a round of lethal cisplatin-paclitaxel combination treatment.Methods: We established an in vitro approach wherein ovarian cancer cells with various sensitivities to cisplatin or paclitaxel were exposed to a round of lethal doses of cisplatin for 1 h plus paclitaxel for 3 h. Thereafter, cells were maintained in media with or without mifepristone, and short- and long-term cytotoxicity was assessed.Results: Four days after treatment the lethality of cisplatin-paclitaxel was evidenced by reduced number of cells, increased hypodiploid DNA content, morphological features of apoptosis, DNA fragmentation, and cleavage of caspase-3, and of its downstream substrate PARP. Short-term presence of mifepristone either enhanced or did not modify such acute lethality. Seven days after receiving cisplatin-paclitaxel, cultures showed signs of relapse with escaping colonies that repopulated the plate in a time-dependent manner. Conversely, cultures exposed to cisplatin-paclitaxel followed by mifepristone not only did not display signs of repopulation following initial chemotherapy, but they also had their clonogenic capacity drastically reduced when compared to cells repopulating after cisplatin-paclitaxel.Conclusions: Cytostatic concentrations of mifepristone after exposure to lethal doses of cisplatin and paclitaxel in combination blocks repopulation of remnant cells surviving and escaping the cytotoxic drugs.Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. University Of South Dakota. Sanford School Of Medicine; Estados UnidosFil: Goyeneche, Alicia A.. University Of South Dakota. Sanford School Of Medicine; Estados UnidosFil: Hapon, María Belén. University Of South Dakota. Sanford School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Telleria, Carlos Marcelo. University Of South Dakota. Sanford School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioMed Central2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81171Gamarra Luques, Carlos Diego; Goyeneche, Alicia A.; Hapon, María Belén; Telleria, Carlos Marcelo; Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy; BioMed Central; BMC Cancer; 12; 5-2012; 1-161471-2407CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-200info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2407-12-200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:59Zoai:ri.conicet.gov.ar:11336/81171instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:59.97CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy |
title |
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy |
spellingShingle |
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy Gamarra Luques, Carlos Diego Mifepristone cancer de ovario apoptosis ciclo celular |
title_short |
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy |
title_full |
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy |
title_fullStr |
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy |
title_full_unstemmed |
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy |
title_sort |
Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy |
dc.creator.none.fl_str_mv |
Gamarra Luques, Carlos Diego Goyeneche, Alicia A. Hapon, María Belén Telleria, Carlos Marcelo |
author |
Gamarra Luques, Carlos Diego |
author_facet |
Gamarra Luques, Carlos Diego Goyeneche, Alicia A. Hapon, María Belén Telleria, Carlos Marcelo |
author_role |
author |
author2 |
Goyeneche, Alicia A. Hapon, María Belén Telleria, Carlos Marcelo |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Mifepristone cancer de ovario apoptosis ciclo celular |
topic |
Mifepristone cancer de ovario apoptosis ciclo celular |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Background: Advanced ovarian cancer is treated with cytoreductive surgery and combination platinum- and taxane-based chemotherapy. Although most patients have acute clinical response to this strategy, the disease ultimately recurs. In this work we questioned whether the synthetic steroid mifepristone, which as monotherapy inhibits the growth of ovarian cancer cells, is capable of preventing repopulation of ovarian cancer cells if given after a round of lethal cisplatin-paclitaxel combination treatment.Methods: We established an in vitro approach wherein ovarian cancer cells with various sensitivities to cisplatin or paclitaxel were exposed to a round of lethal doses of cisplatin for 1 h plus paclitaxel for 3 h. Thereafter, cells were maintained in media with or without mifepristone, and short- and long-term cytotoxicity was assessed.Results: Four days after treatment the lethality of cisplatin-paclitaxel was evidenced by reduced number of cells, increased hypodiploid DNA content, morphological features of apoptosis, DNA fragmentation, and cleavage of caspase-3, and of its downstream substrate PARP. Short-term presence of mifepristone either enhanced or did not modify such acute lethality. Seven days after receiving cisplatin-paclitaxel, cultures showed signs of relapse with escaping colonies that repopulated the plate in a time-dependent manner. Conversely, cultures exposed to cisplatin-paclitaxel followed by mifepristone not only did not display signs of repopulation following initial chemotherapy, but they also had their clonogenic capacity drastically reduced when compared to cells repopulating after cisplatin-paclitaxel.Conclusions: Cytostatic concentrations of mifepristone after exposure to lethal doses of cisplatin and paclitaxel in combination blocks repopulation of remnant cells surviving and escaping the cytotoxic drugs. Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. University Of South Dakota. Sanford School Of Medicine; Estados Unidos Fil: Goyeneche, Alicia A.. University Of South Dakota. Sanford School Of Medicine; Estados Unidos Fil: Hapon, María Belén. University Of South Dakota. Sanford School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Telleria, Carlos Marcelo. University Of South Dakota. Sanford School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Background: Advanced ovarian cancer is treated with cytoreductive surgery and combination platinum- and taxane-based chemotherapy. Although most patients have acute clinical response to this strategy, the disease ultimately recurs. In this work we questioned whether the synthetic steroid mifepristone, which as monotherapy inhibits the growth of ovarian cancer cells, is capable of preventing repopulation of ovarian cancer cells if given after a round of lethal cisplatin-paclitaxel combination treatment.Methods: We established an in vitro approach wherein ovarian cancer cells with various sensitivities to cisplatin or paclitaxel were exposed to a round of lethal doses of cisplatin for 1 h plus paclitaxel for 3 h. Thereafter, cells were maintained in media with or without mifepristone, and short- and long-term cytotoxicity was assessed.Results: Four days after treatment the lethality of cisplatin-paclitaxel was evidenced by reduced number of cells, increased hypodiploid DNA content, morphological features of apoptosis, DNA fragmentation, and cleavage of caspase-3, and of its downstream substrate PARP. Short-term presence of mifepristone either enhanced or did not modify such acute lethality. Seven days after receiving cisplatin-paclitaxel, cultures showed signs of relapse with escaping colonies that repopulated the plate in a time-dependent manner. Conversely, cultures exposed to cisplatin-paclitaxel followed by mifepristone not only did not display signs of repopulation following initial chemotherapy, but they also had their clonogenic capacity drastically reduced when compared to cells repopulating after cisplatin-paclitaxel.Conclusions: Cytostatic concentrations of mifepristone after exposure to lethal doses of cisplatin and paclitaxel in combination blocks repopulation of remnant cells surviving and escaping the cytotoxic drugs. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/81171 Gamarra Luques, Carlos Diego; Goyeneche, Alicia A.; Hapon, María Belén; Telleria, Carlos Marcelo; Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy; BioMed Central; BMC Cancer; 12; 5-2012; 1-16 1471-2407 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/81171 |
identifier_str_mv |
Gamarra Luques, Carlos Diego; Goyeneche, Alicia A.; Hapon, María Belén; Telleria, Carlos Marcelo; Mifepristone prevents repopulation of ovarian cancer cells escaping cisplatin-paclitaxel therapy; BioMed Central; BMC Cancer; 12; 5-2012; 1-16 1471-2407 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-200 info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2407-12-200 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |