Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product

Autores
Maldonado, Natalia Cecilia; Nader, Maria Elena Fatima
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diarrhea in young calves in dairy farms is one of the main causes of economic losses, morbidity and mortality. The use of probiotic products as feed additives or complements is a novel alternative for the prevention of intestinal syndromes. In order to include beneficial bacteria in the design ofa probiotic product, their functional and safety characteristics must be studied. The aim of this work is to evaluate the behavior of the strains in some ?in vitro? gastrointestinal conditions such as acid stress and bile salts in the specific physiological concentration of young calves. The antibioticsusceptibility of a group of lactic acid bacteria from calves which were identified due to their beneficial properties was also studied. The strains, genetically identified and used for the resistance assays were: Lactobacillus johnsonii CRL1692, CRL1693, CRL1699, CRL1700, CRL1701 and CRL1706; L.amylovorus CRL1697; L. murinus CRL 1695 and CRL1705; L. mucosae CRL1696 and CRL1698; L. salivarius CRL1694 and CRL1702; and Enterococcus faecium CRL1703. The results of gut resistance assays showed that all the strains were resistant to pH 4 and to a bile salts concentration of less than 0.5%. However, some of them were sensitive to pH 2. The most pH-sensitive strains were found to be L. johnsonii and L. amylovorus, and enterococci. However, pre-treatment at low pHincreased the growth rate of the L. salivarius strains. The minimal inhibitory concentration showed that the strains were sensitive to Tetracycline, Erythromycin, Chloramphenicol and Ampicillin, while most of them were resistant to Kanamycin. The results allowed the selection of the most adequate strains to be included in a probiotic product that can be utilized most successfully in young calves.
Fil: Maldonado, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
BENEFICIAL LACTIC ACID BACTERIA
NEWBORN CALVES
PROBIOTIC PRODUCTS
FECES
HOST SPECIFICITY
BILE AND ACID TOLERANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28722
Acceder
id CONICETDig_62a4d772946b14c38419fccfa8fa8bab
oai_identifier_str oai:ri.conicet.gov.ar:11336/28722
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic productMaldonado, Natalia CeciliaNader, Maria Elena FatimaBENEFICIAL LACTIC ACID BACTERIANEWBORN CALVESPROBIOTIC PRODUCTSFECESHOST SPECIFICITYBILE AND ACID TOLERANCEhttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Diarrhea in young calves in dairy farms is one of the main causes of economic losses, morbidity and mortality. The use of probiotic products as feed additives or complements is a novel alternative for the prevention of intestinal syndromes. In order to include beneficial bacteria in the design ofa probiotic product, their functional and safety characteristics must be studied. The aim of this work is to evaluate the behavior of the strains in some ?in vitro? gastrointestinal conditions such as acid stress and bile salts in the specific physiological concentration of young calves. The antibioticsusceptibility of a group of lactic acid bacteria from calves which were identified due to their beneficial properties was also studied. The strains, genetically identified and used for the resistance assays were: Lactobacillus johnsonii CRL1692, CRL1693, CRL1699, CRL1700, CRL1701 and CRL1706; L.amylovorus CRL1697; L. murinus CRL 1695 and CRL1705; L. mucosae CRL1696 and CRL1698; L. salivarius CRL1694 and CRL1702; and Enterococcus faecium CRL1703. The results of gut resistance assays showed that all the strains were resistant to pH 4 and to a bile salts concentration of less than 0.5%. However, some of them were sensitive to pH 2. The most pH-sensitive strains were found to be L. johnsonii and L. amylovorus, and enterococci. However, pre-treatment at low pHincreased the growth rate of the L. salivarius strains. The minimal inhibitory concentration showed that the strains were sensitive to Tetracycline, Erythromycin, Chloramphenicol and Ampicillin, while most of them were resistant to Kanamycin. The results allowed the selection of the most adequate strains to be included in a probiotic product that can be utilized most successfully in young calves.Fil: Maldonado, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaPark Publishing group2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28722Maldonado, Natalia Cecilia; Nader, Maria Elena Fatima; Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product; Park Publishing group; International Journal of Veterinary Science and Research; 1; 1; 12-2015; 011-0222410-94442413-8444CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.peertechz.com/Veterinary-Science-Research/pdf/IJVSR-1-104.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:04Zoai:ri.conicet.gov.ar:11336/28722instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:04.319CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
title Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
spellingShingle Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
Maldonado, Natalia Cecilia
BENEFICIAL LACTIC ACID BACTERIA
NEWBORN CALVES
PROBIOTIC PRODUCTS
FECES
HOST SPECIFICITY
BILE AND ACID TOLERANCE
title_short Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
title_full Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
title_fullStr Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
title_full_unstemmed Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
title_sort Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product
dc.creator.none.fl_str_mv Maldonado, Natalia Cecilia
Nader, Maria Elena Fatima
author Maldonado, Natalia Cecilia
author_facet Maldonado, Natalia Cecilia
Nader, Maria Elena Fatima
author_role author
author2 Nader, Maria Elena Fatima
author2_role author
dc.subject.none.fl_str_mv BENEFICIAL LACTIC ACID BACTERIA
NEWBORN CALVES
PROBIOTIC PRODUCTS
FECES
HOST SPECIFICITY
BILE AND ACID TOLERANCE
topic BENEFICIAL LACTIC ACID BACTERIA
NEWBORN CALVES
PROBIOTIC PRODUCTS
FECES
HOST SPECIFICITY
BILE AND ACID TOLERANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Diarrhea in young calves in dairy farms is one of the main causes of economic losses, morbidity and mortality. The use of probiotic products as feed additives or complements is a novel alternative for the prevention of intestinal syndromes. In order to include beneficial bacteria in the design ofa probiotic product, their functional and safety characteristics must be studied. The aim of this work is to evaluate the behavior of the strains in some ?in vitro? gastrointestinal conditions such as acid stress and bile salts in the specific physiological concentration of young calves. The antibioticsusceptibility of a group of lactic acid bacteria from calves which were identified due to their beneficial properties was also studied. The strains, genetically identified and used for the resistance assays were: Lactobacillus johnsonii CRL1692, CRL1693, CRL1699, CRL1700, CRL1701 and CRL1706; L.amylovorus CRL1697; L. murinus CRL 1695 and CRL1705; L. mucosae CRL1696 and CRL1698; L. salivarius CRL1694 and CRL1702; and Enterococcus faecium CRL1703. The results of gut resistance assays showed that all the strains were resistant to pH 4 and to a bile salts concentration of less than 0.5%. However, some of them were sensitive to pH 2. The most pH-sensitive strains were found to be L. johnsonii and L. amylovorus, and enterococci. However, pre-treatment at low pHincreased the growth rate of the L. salivarius strains. The minimal inhibitory concentration showed that the strains were sensitive to Tetracycline, Erythromycin, Chloramphenicol and Ampicillin, while most of them were resistant to Kanamycin. The results allowed the selection of the most adequate strains to be included in a probiotic product that can be utilized most successfully in young calves.
Fil: Maldonado, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Diarrhea in young calves in dairy farms is one of the main causes of economic losses, morbidity and mortality. The use of probiotic products as feed additives or complements is a novel alternative for the prevention of intestinal syndromes. In order to include beneficial bacteria in the design ofa probiotic product, their functional and safety characteristics must be studied. The aim of this work is to evaluate the behavior of the strains in some ?in vitro? gastrointestinal conditions such as acid stress and bile salts in the specific physiological concentration of young calves. The antibioticsusceptibility of a group of lactic acid bacteria from calves which were identified due to their beneficial properties was also studied. The strains, genetically identified and used for the resistance assays were: Lactobacillus johnsonii CRL1692, CRL1693, CRL1699, CRL1700, CRL1701 and CRL1706; L.amylovorus CRL1697; L. murinus CRL 1695 and CRL1705; L. mucosae CRL1696 and CRL1698; L. salivarius CRL1694 and CRL1702; and Enterococcus faecium CRL1703. The results of gut resistance assays showed that all the strains were resistant to pH 4 and to a bile salts concentration of less than 0.5%. However, some of them were sensitive to pH 2. The most pH-sensitive strains were found to be L. johnsonii and L. amylovorus, and enterococci. However, pre-treatment at low pHincreased the growth rate of the L. salivarius strains. The minimal inhibitory concentration showed that the strains were sensitive to Tetracycline, Erythromycin, Chloramphenicol and Ampicillin, while most of them were resistant to Kanamycin. The results allowed the selection of the most adequate strains to be included in a probiotic product that can be utilized most successfully in young calves.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28722
Maldonado, Natalia Cecilia; Nader, Maria Elena Fatima; Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product; Park Publishing group; International Journal of Veterinary Science and Research; 1; 1; 12-2015; 011-022
2410-9444
2413-8444
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28722
identifier_str_mv Maldonado, Natalia Cecilia; Nader, Maria Elena Fatima; Functional Properties (acid and bile tolerance) and antibiotic susceptibility of lactic acid bacteria isolated from newborn calves for the design of a probiotic product; Park Publishing group; International Journal of Veterinary Science and Research; 1; 1; 12-2015; 011-022
2410-9444
2413-8444
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.peertechz.com/Veterinary-Science-Research/pdf/IJVSR-1-104.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Park Publishing group
publisher.none.fl_str_mv Park Publishing group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613777387945984
score 13.070432

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