Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata

Autores
Medesani, Daniel Alberto; Ferre, Lilian Elizabeth; Canosa, Ivana Sofía; Silveyra, Gabriela Romina; Rodriguez, Enrique Marcelo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adult female crabs (Neohelice granulata) were treated during the post-reproductive period with both 17-hydroxyprogesterone (17PG) and methyl farnesoate (MF). During the 4-week in vivo assay, animals were fed hormone-enriched (17PG or MF) pellets twice a week, at a dose of 2 nmol/g of body weight. Additionally, at the middle of the in vivo experiment, ovarian explants were incubated in vitro with each hormone at a concentration of 15 M for 24 h. At the end of the in vivo assay, both the gonadosomatic index and the vitellogenic protein content (Vg) of crabs treated with either 17PG or MF were significantly higher (p 0.05) than the values of the concurrent controls. In addition, the hemolymphatic vitellogenin level of both hormonally treated groups was higher. At the end of the in vitro assay, ovary explants incubated with 17PG had a significantly higher (p 0.05) protein synthesis and content of Vg proteins than controls. Ovary explants incubated with MF had similar levels to controls (p 0.05). The involvement of these hormones in the regulation of ovarian growth during the reproductive cycle of N. granulata is discussed.
Fil: Medesani, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Ferre, Lilian Elizabeth. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Canosa, Ivana Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Silveyra, Gabriela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Rodriguez, Enrique Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Materia
17-Hydroxyprogesterone
Crabs
Methyl Farnesoate
Ovary
Reproduction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63849
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/63849
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulataMedesani, Daniel AlbertoFerre, Lilian ElizabethCanosa, Ivana SofíaSilveyra, Gabriela RominaRodriguez, Enrique Marcelo17-HydroxyprogesteroneCrabsMethyl FarnesoateOvaryReproductionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Adult female crabs (Neohelice granulata) were treated during the post-reproductive period with both 17-hydroxyprogesterone (17PG) and methyl farnesoate (MF). During the 4-week in vivo assay, animals were fed hormone-enriched (17PG or MF) pellets twice a week, at a dose of 2 nmol/g of body weight. Additionally, at the middle of the in vivo experiment, ovarian explants were incubated in vitro with each hormone at a concentration of 15 M for 24 h. At the end of the in vivo assay, both the gonadosomatic index and the vitellogenic protein content (Vg) of crabs treated with either 17PG or MF were significantly higher (p 0.05) than the values of the concurrent controls. In addition, the hemolymphatic vitellogenin level of both hormonally treated groups was higher. At the end of the in vitro assay, ovary explants incubated with 17PG had a significantly higher (p 0.05) protein synthesis and content of Vg proteins than controls. Ovary explants incubated with MF had similar levels to controls (p 0.05). The involvement of these hormones in the regulation of ovarian growth during the reproductive cycle of N. granulata is discussed.Fil: Medesani, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Ferre, Lilian Elizabeth. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Canosa, Ivana Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Silveyra, Gabriela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Rodriguez, Enrique Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaInt Science Services/Balaban Publishers2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63849Medesani, Daniel Alberto; Ferre, Lilian Elizabeth; Canosa, Ivana Sofía; Silveyra, Gabriela Romina; Rodriguez, Enrique Marcelo; Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata; Int Science Services/Balaban Publishers; Invertebrate Reproduction & Development; 59; 2; 4-2015; 104-1100792-4259CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/07924259.2015.1019014info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/07924259.2015.1019014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:15:16Zoai:ri.conicet.gov.ar:11336/63849instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:15:17.235CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata
title Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata
spellingShingle Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata
Medesani, Daniel Alberto
17-Hydroxyprogesterone
Crabs
Methyl Farnesoate
Ovary
Reproduction
title_short Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata
title_full Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata
title_fullStr Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata
title_full_unstemmed Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata
title_sort Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata
dc.creator.none.fl_str_mv Medesani, Daniel Alberto
Ferre, Lilian Elizabeth
Canosa, Ivana Sofía
Silveyra, Gabriela Romina
Rodriguez, Enrique Marcelo
author Medesani, Daniel Alberto
author_facet Medesani, Daniel Alberto
Ferre, Lilian Elizabeth
Canosa, Ivana Sofía
Silveyra, Gabriela Romina
Rodriguez, Enrique Marcelo
author_role author
author2 Ferre, Lilian Elizabeth
Canosa, Ivana Sofía
Silveyra, Gabriela Romina
Rodriguez, Enrique Marcelo
author2_role author
author
author
author
dc.subject.none.fl_str_mv 17-Hydroxyprogesterone
Crabs
Methyl Farnesoate
Ovary
Reproduction
topic 17-Hydroxyprogesterone
Crabs
Methyl Farnesoate
Ovary
Reproduction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Adult female crabs (Neohelice granulata) were treated during the post-reproductive period with both 17-hydroxyprogesterone (17PG) and methyl farnesoate (MF). During the 4-week in vivo assay, animals were fed hormone-enriched (17PG or MF) pellets twice a week, at a dose of 2 nmol/g of body weight. Additionally, at the middle of the in vivo experiment, ovarian explants were incubated in vitro with each hormone at a concentration of 15 M for 24 h. At the end of the in vivo assay, both the gonadosomatic index and the vitellogenic protein content (Vg) of crabs treated with either 17PG or MF were significantly higher (p 0.05) than the values of the concurrent controls. In addition, the hemolymphatic vitellogenin level of both hormonally treated groups was higher. At the end of the in vitro assay, ovary explants incubated with 17PG had a significantly higher (p 0.05) protein synthesis and content of Vg proteins than controls. Ovary explants incubated with MF had similar levels to controls (p 0.05). The involvement of these hormones in the regulation of ovarian growth during the reproductive cycle of N. granulata is discussed.
Fil: Medesani, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Ferre, Lilian Elizabeth. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Canosa, Ivana Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Silveyra, Gabriela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Rodriguez, Enrique Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
description Adult female crabs (Neohelice granulata) were treated during the post-reproductive period with both 17-hydroxyprogesterone (17PG) and methyl farnesoate (MF). During the 4-week in vivo assay, animals were fed hormone-enriched (17PG or MF) pellets twice a week, at a dose of 2 nmol/g of body weight. Additionally, at the middle of the in vivo experiment, ovarian explants were incubated in vitro with each hormone at a concentration of 15 M for 24 h. At the end of the in vivo assay, both the gonadosomatic index and the vitellogenic protein content (Vg) of crabs treated with either 17PG or MF were significantly higher (p 0.05) than the values of the concurrent controls. In addition, the hemolymphatic vitellogenin level of both hormonally treated groups was higher. At the end of the in vitro assay, ovary explants incubated with 17PG had a significantly higher (p 0.05) protein synthesis and content of Vg proteins than controls. Ovary explants incubated with MF had similar levels to controls (p 0.05). The involvement of these hormones in the regulation of ovarian growth during the reproductive cycle of N. granulata is discussed.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63849
Medesani, Daniel Alberto; Ferre, Lilian Elizabeth; Canosa, Ivana Sofía; Silveyra, Gabriela Romina; Rodriguez, Enrique Marcelo; Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata; Int Science Services/Balaban Publishers; Invertebrate Reproduction & Development; 59; 2; 4-2015; 104-110
0792-4259
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63849
identifier_str_mv Medesani, Daniel Alberto; Ferre, Lilian Elizabeth; Canosa, Ivana Sofía; Silveyra, Gabriela Romina; Rodriguez, Enrique Marcelo; Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crab Neohelice granulata; Int Science Services/Balaban Publishers; Invertebrate Reproduction & Development; 59; 2; 4-2015; 104-110
0792-4259
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/07924259.2015.1019014
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/07924259.2015.1019014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Int Science Services/Balaban Publishers
publisher.none.fl_str_mv Int Science Services/Balaban Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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