Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen

Autores
Demonte, Ana M.; Asención Diez, Matías Damián; Guerrero, Sergio Adrian; Ballicora, Miguel A.; Iglesias, Alberto Alvaro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The presence of intracellular glycogen can be detected by the following iodine staining technique. Cells with glycogen stain dark brown, whereas in its absence they remain with a pale yellowish color. It is hypothesized that iodine atoms fit into helical coils formed by the α-polyglucan to form a coloured glycogen-iodine complex. Here, we have studied the expression of Streptococcus mutans (S. mutans) genes that control the biosynthesis of this polysaccharide. Thus, we expressed glgC and glgD genes coding for both ADP-Glc pyrophosphorylase subunits in Escherichia coli (E. coli) AC70R1-504 cells to complement the deficient accumulation of glycogen by this strain. In control cells or in those where an inactive protein was expressed, the synthesis of the polysaccharide was undetectable by this iodine staining technique.
Fil: Demonte, Ana M.. Universidad Nacional del Litoral; Argentina
Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
Fil: Ballicora, Miguel A.. Loyola University Chicago. Department of Chemistry & Biochemistry; Estados Unidos
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina
Materia
Polysaccharide detection
Bacteria carbohydrate
Glycogen metabolism
Polyglucan storage
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13367
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial GlycogenDemonte, Ana M.Asención Diez, Matías DamiánGuerrero, Sergio AdrianBallicora, Miguel A.Iglesias, Alberto AlvaroPolysaccharide detectionBacteria carbohydrateGlycogen metabolismPolyglucan storagehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The presence of intracellular glycogen can be detected by the following iodine staining technique. Cells with glycogen stain dark brown, whereas in its absence they remain with a pale yellowish color. It is hypothesized that iodine atoms fit into helical coils formed by the α-polyglucan to form a coloured glycogen-iodine complex. Here, we have studied the expression of Streptococcus mutans (S. mutans) genes that control the biosynthesis of this polysaccharide. Thus, we expressed glgC and glgD genes coding for both ADP-Glc pyrophosphorylase subunits in Escherichia coli (E. coli) AC70R1-504 cells to complement the deficient accumulation of glycogen by this strain. In control cells or in those where an inactive protein was expressed, the synthesis of the polysaccharide was undetectable by this iodine staining technique.Fil: Demonte, Ana M.. Universidad Nacional del Litoral; ArgentinaFil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; ArgentinaFil: Ballicora, Miguel A.. Loyola University Chicago. Department of Chemistry & Biochemistry; Estados UnidosFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; ArgentinaBioProtocol Inc.2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13367Demonte, Ana M.; Asención Diez, Matías Damián; Guerrero, Sergio Adrian; Ballicora, Miguel A.; Iglesias, Alberto Alvaro; Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen; BioProtocol Inc.; Bio-protocol; 4; 17; 9-2014; 25582331-8325enginfo:eu-repo/semantics/altIdentifier/url/http://www.bio-protocol.org/e1224info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.1224info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:20:47Zoai:ri.conicet.gov.ar:11336/13367instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:20:48.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
title Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
spellingShingle Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
Demonte, Ana M.
Polysaccharide detection
Bacteria carbohydrate
Glycogen metabolism
Polyglucan storage
title_short Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
title_full Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
title_fullStr Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
title_full_unstemmed Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
title_sort Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
dc.creator.none.fl_str_mv Demonte, Ana M.
Asención Diez, Matías Damián
Guerrero, Sergio Adrian
Ballicora, Miguel A.
Iglesias, Alberto Alvaro
author Demonte, Ana M.
author_facet Demonte, Ana M.
Asención Diez, Matías Damián
Guerrero, Sergio Adrian
Ballicora, Miguel A.
Iglesias, Alberto Alvaro
author_role author
author2 Asención Diez, Matías Damián
Guerrero, Sergio Adrian
Ballicora, Miguel A.
Iglesias, Alberto Alvaro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Polysaccharide detection
Bacteria carbohydrate
Glycogen metabolism
Polyglucan storage
topic Polysaccharide detection
Bacteria carbohydrate
Glycogen metabolism
Polyglucan storage
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The presence of intracellular glycogen can be detected by the following iodine staining technique. Cells with glycogen stain dark brown, whereas in its absence they remain with a pale yellowish color. It is hypothesized that iodine atoms fit into helical coils formed by the α-polyglucan to form a coloured glycogen-iodine complex. Here, we have studied the expression of Streptococcus mutans (S. mutans) genes that control the biosynthesis of this polysaccharide. Thus, we expressed glgC and glgD genes coding for both ADP-Glc pyrophosphorylase subunits in Escherichia coli (E. coli) AC70R1-504 cells to complement the deficient accumulation of glycogen by this strain. In control cells or in those where an inactive protein was expressed, the synthesis of the polysaccharide was undetectable by this iodine staining technique.
Fil: Demonte, Ana M.. Universidad Nacional del Litoral; Argentina
Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
Fil: Ballicora, Miguel A.. Loyola University Chicago. Department of Chemistry & Biochemistry; Estados Unidos
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina
description The presence of intracellular glycogen can be detected by the following iodine staining technique. Cells with glycogen stain dark brown, whereas in its absence they remain with a pale yellowish color. It is hypothesized that iodine atoms fit into helical coils formed by the α-polyglucan to form a coloured glycogen-iodine complex. Here, we have studied the expression of Streptococcus mutans (S. mutans) genes that control the biosynthesis of this polysaccharide. Thus, we expressed glgC and glgD genes coding for both ADP-Glc pyrophosphorylase subunits in Escherichia coli (E. coli) AC70R1-504 cells to complement the deficient accumulation of glycogen by this strain. In control cells or in those where an inactive protein was expressed, the synthesis of the polysaccharide was undetectable by this iodine staining technique.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13367
Demonte, Ana M.; Asención Diez, Matías Damián; Guerrero, Sergio Adrian; Ballicora, Miguel A.; Iglesias, Alberto Alvaro; Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen; BioProtocol Inc.; Bio-protocol; 4; 17; 9-2014; 2558
2331-8325
url http://hdl.handle.net/11336/13367
identifier_str_mv Demonte, Ana M.; Asención Diez, Matías Damián; Guerrero, Sergio Adrian; Ballicora, Miguel A.; Iglesias, Alberto Alvaro; Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen; BioProtocol Inc.; Bio-protocol; 4; 17; 9-2014; 2558
2331-8325
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.bio-protocol.org/e1224
info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.1224
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioProtocol Inc.
publisher.none.fl_str_mv BioProtocol Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083356582739968
score 13.22299

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