Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells

Autores
Verstraeten, Sandra Viviana; Zago, María Paola; Mackenzie, Gerardo G.; Keen, Carl L.; Oteiza, Patricia Isabel
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We investigated whether zinc deficiency can affect plasma membrane rheology. Three cell lines, human leukaemia T-cells (Jurkat), rat fibroblasts (3T3) and human neuroblastoma cells (IMR-32), were cultured for 48 h in control medium, in zinc-deficient medium (1.5 μM zinc; 1.5 Zn), or in the zinc-deficient medium supplemented with 15 μM zinc (15 Zn). The number of viable cells was lower in the 1.5 Zn group than in the control and 15 Zn groups. The frequency of apoptosis was higher in the 1.5 Zn group than in the control and 15 Zn groups. Membrane fluidity was evaluated using the 6-(9-anthroyloxy) stearic acid and 16-(9-anthroyloxy)palmitic acid probes. Membrane fluidity was higher in 1.5 Zn cells than in the control cells; no differences were observed between control cells and 15 Zn cells. The effect of zinc deficiency on membrane fluidity at the water/lipid interface was associated with a higher phosphatidylserine externalization. The higher membrane fluidity in the hydrophobic region of the bilayer was correlated with a lower content of arachidonic acid. We suggest that the increased fluidity of the membrane secondary to zinc deficiency is in part due to a decrease in arachidonic acid content and the apoptosis-related changes in phosphatidylserine distribution.
Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Mackenzie, Gerardo G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Keen, Carl L.. University of California at Davis; Estados Unidos
Fil: Oteiza, Patricia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; Argentina. University of California at Davis; Estados Unidos
Materia
APOPTOSIS
MEMBRANE FLUIDITY
PHOSPHATIDYLSERINE
ZINC
ZINC DEFICIENCY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98033
Acceder
id CONICETDig_6237a87ce0feb10bc866667195226bef
oai_identifier_str oai:ri.conicet.gov.ar:11336/98033
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cellsVerstraeten, Sandra VivianaZago, María PaolaMackenzie, Gerardo G.Keen, Carl L.Oteiza, Patricia IsabelAPOPTOSISMEMBRANE FLUIDITYPHOSPHATIDYLSERINEZINCZINC DEFICIENCYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We investigated whether zinc deficiency can affect plasma membrane rheology. Three cell lines, human leukaemia T-cells (Jurkat), rat fibroblasts (3T3) and human neuroblastoma cells (IMR-32), were cultured for 48 h in control medium, in zinc-deficient medium (1.5 μM zinc; 1.5 Zn), or in the zinc-deficient medium supplemented with 15 μM zinc (15 Zn). The number of viable cells was lower in the 1.5 Zn group than in the control and 15 Zn groups. The frequency of apoptosis was higher in the 1.5 Zn group than in the control and 15 Zn groups. Membrane fluidity was evaluated using the 6-(9-anthroyloxy) stearic acid and 16-(9-anthroyloxy)palmitic acid probes. Membrane fluidity was higher in 1.5 Zn cells than in the control cells; no differences were observed between control cells and 15 Zn cells. The effect of zinc deficiency on membrane fluidity at the water/lipid interface was associated with a higher phosphatidylserine externalization. The higher membrane fluidity in the hydrophobic region of the bilayer was correlated with a lower content of arachidonic acid. We suggest that the increased fluidity of the membrane secondary to zinc deficiency is in part due to a decrease in arachidonic acid content and the apoptosis-related changes in phosphatidylserine distribution.Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Mackenzie, Gerardo G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Keen, Carl L.. University of California at Davis; Estados UnidosFil: Oteiza, Patricia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; Argentina. University of California at Davis; Estados UnidosPortland Press2004-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98033Verstraeten, Sandra Viviana; Zago, María Paola; Mackenzie, Gerardo G.; Keen, Carl L.; Oteiza, Patricia Isabel; Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells; Portland Press; Biochemical Journal; 378; 2; 3-2004; 579-5870264-6021CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1042/BJ20031054info:eu-repo/semantics/altIdentifier/url/https://portlandpress.com/biochemj/article-abstract/378/2/579/41269/Influence-of-zinc-deficiency-on-cell-membrane?redirectedFrom=fulltextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:05:30Zoai:ri.conicet.gov.ar:11336/98033instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:05:30.932CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells
title Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells
spellingShingle Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells
Verstraeten, Sandra Viviana
APOPTOSIS
MEMBRANE FLUIDITY
PHOSPHATIDYLSERINE
ZINC
ZINC DEFICIENCY
title_short Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells
title_full Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells
title_fullStr Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells
title_full_unstemmed Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells
title_sort Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells
dc.creator.none.fl_str_mv Verstraeten, Sandra Viviana
Zago, María Paola
Mackenzie, Gerardo G.
Keen, Carl L.
Oteiza, Patricia Isabel
author Verstraeten, Sandra Viviana
author_facet Verstraeten, Sandra Viviana
Zago, María Paola
Mackenzie, Gerardo G.
Keen, Carl L.
Oteiza, Patricia Isabel
author_role author
author2 Zago, María Paola
Mackenzie, Gerardo G.
Keen, Carl L.
Oteiza, Patricia Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
MEMBRANE FLUIDITY
PHOSPHATIDYLSERINE
ZINC
ZINC DEFICIENCY
topic APOPTOSIS
MEMBRANE FLUIDITY
PHOSPHATIDYLSERINE
ZINC
ZINC DEFICIENCY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We investigated whether zinc deficiency can affect plasma membrane rheology. Three cell lines, human leukaemia T-cells (Jurkat), rat fibroblasts (3T3) and human neuroblastoma cells (IMR-32), were cultured for 48 h in control medium, in zinc-deficient medium (1.5 μM zinc; 1.5 Zn), or in the zinc-deficient medium supplemented with 15 μM zinc (15 Zn). The number of viable cells was lower in the 1.5 Zn group than in the control and 15 Zn groups. The frequency of apoptosis was higher in the 1.5 Zn group than in the control and 15 Zn groups. Membrane fluidity was evaluated using the 6-(9-anthroyloxy) stearic acid and 16-(9-anthroyloxy)palmitic acid probes. Membrane fluidity was higher in 1.5 Zn cells than in the control cells; no differences were observed between control cells and 15 Zn cells. The effect of zinc deficiency on membrane fluidity at the water/lipid interface was associated with a higher phosphatidylserine externalization. The higher membrane fluidity in the hydrophobic region of the bilayer was correlated with a lower content of arachidonic acid. We suggest that the increased fluidity of the membrane secondary to zinc deficiency is in part due to a decrease in arachidonic acid content and the apoptosis-related changes in phosphatidylserine distribution.
Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Mackenzie, Gerardo G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Keen, Carl L.. University of California at Davis; Estados Unidos
Fil: Oteiza, Patricia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires; Argentina. University of California at Davis; Estados Unidos
description We investigated whether zinc deficiency can affect plasma membrane rheology. Three cell lines, human leukaemia T-cells (Jurkat), rat fibroblasts (3T3) and human neuroblastoma cells (IMR-32), were cultured for 48 h in control medium, in zinc-deficient medium (1.5 μM zinc; 1.5 Zn), or in the zinc-deficient medium supplemented with 15 μM zinc (15 Zn). The number of viable cells was lower in the 1.5 Zn group than in the control and 15 Zn groups. The frequency of apoptosis was higher in the 1.5 Zn group than in the control and 15 Zn groups. Membrane fluidity was evaluated using the 6-(9-anthroyloxy) stearic acid and 16-(9-anthroyloxy)palmitic acid probes. Membrane fluidity was higher in 1.5 Zn cells than in the control cells; no differences were observed between control cells and 15 Zn cells. The effect of zinc deficiency on membrane fluidity at the water/lipid interface was associated with a higher phosphatidylserine externalization. The higher membrane fluidity in the hydrophobic region of the bilayer was correlated with a lower content of arachidonic acid. We suggest that the increased fluidity of the membrane secondary to zinc deficiency is in part due to a decrease in arachidonic acid content and the apoptosis-related changes in phosphatidylserine distribution.
publishDate 2004
dc.date.none.fl_str_mv 2004-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98033
Verstraeten, Sandra Viviana; Zago, María Paola; Mackenzie, Gerardo G.; Keen, Carl L.; Oteiza, Patricia Isabel; Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells; Portland Press; Biochemical Journal; 378; 2; 3-2004; 579-587
0264-6021
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98033
identifier_str_mv Verstraeten, Sandra Viviana; Zago, María Paola; Mackenzie, Gerardo G.; Keen, Carl L.; Oteiza, Patricia Isabel; Influence of zinc deficiency on cell-membrane fluidity in Jurkat, 3T3 and IMR-32 cells; Portland Press; Biochemical Journal; 378; 2; 3-2004; 579-587
0264-6021
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1042/BJ20031054
info:eu-repo/semantics/altIdentifier/url/https://portlandpress.com/biochemj/article-abstract/378/2/579/41269/Influence-of-zinc-deficiency-on-cell-membrane?redirectedFrom=fulltext
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Portland Press
publisher.none.fl_str_mv Portland Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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