In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
- Autores
- Alonso, Ana Elisa; Baca Castex, Clara; Ferrante, Alejandro Antonio; Pinto, Marcelo Raul; Castaneira, Catalina; Trasorras, Virginia Luz; Gambarotta, Mariana; Losinno, Luis; Miragaya, Marcelo
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (control) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare′s oviduct) cultured for 7 days. With in vitro culture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be produced using air-dried spermatozoa stored for several weeks. Sperm extract activation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved.
Fil: Alonso, Ana Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Pinto, Marcelo Raul. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Castaneira, Catalina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina
Fil: Trasorras, Virginia Luz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Losinno, Luis. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina - Materia
-
Air-Dried Spermatozoa
Equine
Icsi
Invivoculture
Sperm Extrac - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/33431
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/33431 |
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CONICET Digital (CONICET) |
spelling |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systemsAlonso, Ana ElisaBaca Castex, ClaraFerrante, Alejandro AntonioPinto, Marcelo RaulCastaneira, CatalinaTrasorras, Virginia LuzGambarotta, MarianaLosinno, LuisMiragaya, MarceloAir-Dried SpermatozoaEquineIcsiInvivocultureSperm Extrachttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (control) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare′s oviduct) cultured for 7 days. With in vitro culture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be produced using air-dried spermatozoa stored for several weeks. Sperm extract activation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved.Fil: Alonso, Ana Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Pinto, Marcelo Raul. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Castaneira, Catalina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; ArgentinaFil: Trasorras, Virginia Luz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Losinno, Luis. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; ArgentinaFil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaWiley Blackwell Publishing, Inc2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33431Ferrante, Alejandro Antonio; Alonso, Ana Elisa; Castaneira, Catalina; Baca Castex, Clara; Losinno, Luis; Gambarotta, Mariana; et al.; In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems; Wiley Blackwell Publishing, Inc; Andrologia; 47; 4; 3-2014; 387-3940303-4569CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/and.12273info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/and.12273/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:18Zoai:ri.conicet.gov.ar:11336/33431instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:19.033CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems |
title |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems |
spellingShingle |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems Alonso, Ana Elisa Air-Dried Spermatozoa Equine Icsi Invivoculture Sperm Extrac |
title_short |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems |
title_full |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems |
title_fullStr |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems |
title_full_unstemmed |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems |
title_sort |
In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems |
dc.creator.none.fl_str_mv |
Alonso, Ana Elisa Baca Castex, Clara Ferrante, Alejandro Antonio Pinto, Marcelo Raul Castaneira, Catalina Trasorras, Virginia Luz Gambarotta, Mariana Losinno, Luis Miragaya, Marcelo |
author |
Alonso, Ana Elisa |
author_facet |
Alonso, Ana Elisa Baca Castex, Clara Ferrante, Alejandro Antonio Pinto, Marcelo Raul Castaneira, Catalina Trasorras, Virginia Luz Gambarotta, Mariana Losinno, Luis Miragaya, Marcelo |
author_role |
author |
author2 |
Baca Castex, Clara Ferrante, Alejandro Antonio Pinto, Marcelo Raul Castaneira, Catalina Trasorras, Virginia Luz Gambarotta, Mariana Losinno, Luis Miragaya, Marcelo |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Air-Dried Spermatozoa Equine Icsi Invivoculture Sperm Extrac |
topic |
Air-Dried Spermatozoa Equine Icsi Invivoculture Sperm Extrac |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (control) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare′s oviduct) cultured for 7 days. With in vitro culture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be produced using air-dried spermatozoa stored for several weeks. Sperm extract activation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved. Fil: Alonso, Ana Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Pinto, Marcelo Raul. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Castaneira, Catalina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina Fil: Trasorras, Virginia Luz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Losinno, Luis. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina |
description |
The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (control) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare′s oviduct) cultured for 7 days. With in vitro culture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be produced using air-dried spermatozoa stored for several weeks. Sperm extract activation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/33431 Ferrante, Alejandro Antonio; Alonso, Ana Elisa; Castaneira, Catalina; Baca Castex, Clara; Losinno, Luis; Gambarotta, Mariana; et al.; In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems; Wiley Blackwell Publishing, Inc; Andrologia; 47; 4; 3-2014; 387-394 0303-4569 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/33431 |
identifier_str_mv |
Ferrante, Alejandro Antonio; Alonso, Ana Elisa; Castaneira, Catalina; Baca Castex, Clara; Losinno, Luis; Gambarotta, Mariana; et al.; In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems; Wiley Blackwell Publishing, Inc; Andrologia; 47; 4; 3-2014; 387-394 0303-4569 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/and.12273 info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/and.12273/abstract |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614371132571648 |
score |
13.070432 |