In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems

Autores
Alonso, Ana Elisa; Baca Castex, Clara; Ferrante, Alejandro Antonio; Pinto, Marcelo Raul; Castaneira, Catalina; Trasorras, Virginia Luz; Gambarotta, Mariana; Losinno, Luis; Miragaya, Marcelo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (control) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare′s oviduct) cultured for 7 days. With in vitro culture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be produced using air-dried spermatozoa stored for several weeks. Sperm extract activation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved.
Fil: Alonso, Ana Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Pinto, Marcelo Raul. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Castaneira, Catalina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina
Fil: Trasorras, Virginia Luz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Losinno, Luis. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Materia
Air-Dried Spermatozoa
Equine
Icsi
Invivoculture
Sperm Extrac
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33431
Acceder
id CONICETDig_61f3b158f4f327c10a434ec76de14e95
oai_identifier_str oai:ri.conicet.gov.ar:11336/33431
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systemsAlonso, Ana ElisaBaca Castex, ClaraFerrante, Alejandro AntonioPinto, Marcelo RaulCastaneira, CatalinaTrasorras, Virginia LuzGambarotta, MarianaLosinno, LuisMiragaya, MarceloAir-Dried SpermatozoaEquineIcsiInvivocultureSperm Extrachttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (control) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare′s oviduct) cultured for 7 days. With in vitro culture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be produced using air-dried spermatozoa stored for several weeks. Sperm extract activation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved.Fil: Alonso, Ana Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Pinto, Marcelo Raul. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Castaneira, Catalina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; ArgentinaFil: Trasorras, Virginia Luz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Losinno, Luis. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; ArgentinaFil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaWiley Blackwell Publishing, Inc2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33431Ferrante, Alejandro Antonio; Alonso, Ana Elisa; Castaneira, Catalina; Baca Castex, Clara; Losinno, Luis; Gambarotta, Mariana; et al.; In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems; Wiley Blackwell Publishing, Inc; Andrologia; 47; 4; 3-2014; 387-3940303-4569CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/and.12273info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/and.12273/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:32:53Zoai:ri.conicet.gov.ar:11336/33431instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:32:53.877CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
title In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
spellingShingle In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
Alonso, Ana Elisa
Air-Dried Spermatozoa
Equine
Icsi
Invivoculture
Sperm Extrac
title_short In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
title_full In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
title_fullStr In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
title_full_unstemmed In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
title_sort In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
dc.creator.none.fl_str_mv Alonso, Ana Elisa
Baca Castex, Clara
Ferrante, Alejandro Antonio
Pinto, Marcelo Raul
Castaneira, Catalina
Trasorras, Virginia Luz
Gambarotta, Mariana
Losinno, Luis
Miragaya, Marcelo
author Alonso, Ana Elisa
author_facet Alonso, Ana Elisa
Baca Castex, Clara
Ferrante, Alejandro Antonio
Pinto, Marcelo Raul
Castaneira, Catalina
Trasorras, Virginia Luz
Gambarotta, Mariana
Losinno, Luis
Miragaya, Marcelo
author_role author
author2 Baca Castex, Clara
Ferrante, Alejandro Antonio
Pinto, Marcelo Raul
Castaneira, Catalina
Trasorras, Virginia Luz
Gambarotta, Mariana
Losinno, Luis
Miragaya, Marcelo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Air-Dried Spermatozoa
Equine
Icsi
Invivoculture
Sperm Extrac
topic Air-Dried Spermatozoa
Equine
Icsi
Invivoculture
Sperm Extrac
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (control) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare′s oviduct) cultured for 7 days. With in vitro culture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be produced using air-dried spermatozoa stored for several weeks. Sperm extract activation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved.
Fil: Alonso, Ana Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Pinto, Marcelo Raul. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Castaneira, Catalina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina
Fil: Trasorras, Virginia Luz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Losinno, Luis. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
description The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (control) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare′s oviduct) cultured for 7 days. With in vitro culture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be produced using air-dried spermatozoa stored for several weeks. Sperm extract activation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33431
Ferrante, Alejandro Antonio; Alonso, Ana Elisa; Castaneira, Catalina; Baca Castex, Clara; Losinno, Luis; Gambarotta, Mariana; et al.; In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems; Wiley Blackwell Publishing, Inc; Andrologia; 47; 4; 3-2014; 387-394
0303-4569
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33431
identifier_str_mv Ferrante, Alejandro Antonio; Alonso, Ana Elisa; Castaneira, Catalina; Baca Castex, Clara; Losinno, Luis; Gambarotta, Mariana; et al.; In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems; Wiley Blackwell Publishing, Inc; Andrologia; 47; 4; 3-2014; 387-394
0303-4569
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/and.12273
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/and.12273/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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