Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application

Autores
Druille, Magdalena; Omacini, Marina; Golluscio, Rodolfo; Cabello, Marta Noemi
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glyphosate is a systemic non-selective herbicide, the most widely used in the world. Alongside with itsuse in agricultural and forestry systems, this herbicide is used in grasslands in late summer with theaim of promoting winter species with the consequent increase in stocking rate. However, its effects onnon-target organisms, such as arbuscular mycorrhizal fungi (AMF), are unclear. Arbuscular mycorrhizalfungi (AMF) colonize the root of more than 80% of terrestrial plants, improving their growth and survival,and therefore playing a key role in ecosystem structure and function. The aim of this work was to inves-tigate the possible pathways through which glyphosate application affects AMF spores viability and rootcolonization in grassland communities. Our hypothesis is that glyphosate application can damage AMFdirectly (through contact with spores and external hyphae) or indirectly through the changes it generateson host plants. The experiment had a factorial array with three factors: (1) plant species, at two levels(Paspalum dilatatum and Lotus tenuis), (2) doses of glyphosate, at three levels (0 l ha−1, 0.8 l ha−1and 3 l ha−1), and (3) application site, at two levels: soil (direct pathway) and plant foliage (indirect pathway).Spore viability was reduced even under the lowest glyphosate rate, but only when it was applied on thesoil. Total root colonization for both species was similarly decreased when glyphosate was applied toplant foliage or on soil, with no difference between 0.8 and 3 l ha−1. The number of arbuscules was 20%lower when glyphosate was applied on plant foliage, than when it was applied on the soil. Our findingsillustrate that glyphosate application negatively affects AMF functionality in grasslands, due to differentcauses depending on the herbicide application site. While, under field conditions, the occurrence of directand/or indirect pathways will depend on the plant cover at the time of glyphosate application, the con-sequences of this practice on the plant community structure will vary with the mycorrhizal dependenceof the species composition regardless of the pathway involved.
Fil: Druille, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Omacini, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Golluscio, Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Cabello, Marta Noemi. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Materia
Amf Propagules
Grasslands
Non-Target Organisms
Root Colonization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4187
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4187
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate applicationDruille, MagdalenaOmacini, MarinaGolluscio, RodolfoCabello, Marta NoemiAmf PropagulesGrasslandsNon-Target OrganismsRoot Colonizationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Glyphosate is a systemic non-selective herbicide, the most widely used in the world. Alongside with itsuse in agricultural and forestry systems, this herbicide is used in grasslands in late summer with theaim of promoting winter species with the consequent increase in stocking rate. However, its effects onnon-target organisms, such as arbuscular mycorrhizal fungi (AMF), are unclear. Arbuscular mycorrhizalfungi (AMF) colonize the root of more than 80% of terrestrial plants, improving their growth and survival,and therefore playing a key role in ecosystem structure and function. The aim of this work was to inves-tigate the possible pathways through which glyphosate application affects AMF spores viability and rootcolonization in grassland communities. Our hypothesis is that glyphosate application can damage AMFdirectly (through contact with spores and external hyphae) or indirectly through the changes it generateson host plants. The experiment had a factorial array with three factors: (1) plant species, at two levels(Paspalum dilatatum and Lotus tenuis), (2) doses of glyphosate, at three levels (0 l ha−1, 0.8 l ha−1and 3 l ha−1), and (3) application site, at two levels: soil (direct pathway) and plant foliage (indirect pathway).Spore viability was reduced even under the lowest glyphosate rate, but only when it was applied on thesoil. Total root colonization for both species was similarly decreased when glyphosate was applied toplant foliage or on soil, with no difference between 0.8 and 3 l ha−1. The number of arbuscules was 20%lower when glyphosate was applied on plant foliage, than when it was applied on the soil. Our findingsillustrate that glyphosate application negatively affects AMF functionality in grasslands, due to differentcauses depending on the herbicide application site. While, under field conditions, the occurrence of directand/or indirect pathways will depend on the plant cover at the time of glyphosate application, the con-sequences of this practice on the plant community structure will vary with the mycorrhizal dependenceof the species composition regardless of the pathway involved.Fil: Druille, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Omacini, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Golluscio, Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Cabello, Marta Noemi. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaElsevier2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4187Druille, Magdalena; Omacini, Marina; Golluscio, Rodolfo; Cabello, Marta Noemi; Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application; Elsevier; Applied Soil Ecology; 72; 6-2013; 143-1490929-1393enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2013.06.011info:eu-repo/semantics/altIdentifier/issn/0929-1393info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139313001868info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:38Zoai:ri.conicet.gov.ar:11336/4187instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:38.792CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application
title Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application
spellingShingle Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application
Druille, Magdalena
Amf Propagules
Grasslands
Non-Target Organisms
Root Colonization
title_short Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application
title_full Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application
title_fullStr Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application
title_full_unstemmed Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application
title_sort Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application
dc.creator.none.fl_str_mv Druille, Magdalena
Omacini, Marina
Golluscio, Rodolfo
Cabello, Marta Noemi
author Druille, Magdalena
author_facet Druille, Magdalena
Omacini, Marina
Golluscio, Rodolfo
Cabello, Marta Noemi
author_role author
author2 Omacini, Marina
Golluscio, Rodolfo
Cabello, Marta Noemi
author2_role author
author
author
dc.subject.none.fl_str_mv Amf Propagules
Grasslands
Non-Target Organisms
Root Colonization
topic Amf Propagules
Grasslands
Non-Target Organisms
Root Colonization
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Glyphosate is a systemic non-selective herbicide, the most widely used in the world. Alongside with itsuse in agricultural and forestry systems, this herbicide is used in grasslands in late summer with theaim of promoting winter species with the consequent increase in stocking rate. However, its effects onnon-target organisms, such as arbuscular mycorrhizal fungi (AMF), are unclear. Arbuscular mycorrhizalfungi (AMF) colonize the root of more than 80% of terrestrial plants, improving their growth and survival,and therefore playing a key role in ecosystem structure and function. The aim of this work was to inves-tigate the possible pathways through which glyphosate application affects AMF spores viability and rootcolonization in grassland communities. Our hypothesis is that glyphosate application can damage AMFdirectly (through contact with spores and external hyphae) or indirectly through the changes it generateson host plants. The experiment had a factorial array with three factors: (1) plant species, at two levels(Paspalum dilatatum and Lotus tenuis), (2) doses of glyphosate, at three levels (0 l ha−1, 0.8 l ha−1and 3 l ha−1), and (3) application site, at two levels: soil (direct pathway) and plant foliage (indirect pathway).Spore viability was reduced even under the lowest glyphosate rate, but only when it was applied on thesoil. Total root colonization for both species was similarly decreased when glyphosate was applied toplant foliage or on soil, with no difference between 0.8 and 3 l ha−1. The number of arbuscules was 20%lower when glyphosate was applied on plant foliage, than when it was applied on the soil. Our findingsillustrate that glyphosate application negatively affects AMF functionality in grasslands, due to differentcauses depending on the herbicide application site. While, under field conditions, the occurrence of directand/or indirect pathways will depend on the plant cover at the time of glyphosate application, the con-sequences of this practice on the plant community structure will vary with the mycorrhizal dependenceof the species composition regardless of the pathway involved.
Fil: Druille, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Omacini, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Golluscio, Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Cabello, Marta Noemi. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
description Glyphosate is a systemic non-selective herbicide, the most widely used in the world. Alongside with itsuse in agricultural and forestry systems, this herbicide is used in grasslands in late summer with theaim of promoting winter species with the consequent increase in stocking rate. However, its effects onnon-target organisms, such as arbuscular mycorrhizal fungi (AMF), are unclear. Arbuscular mycorrhizalfungi (AMF) colonize the root of more than 80% of terrestrial plants, improving their growth and survival,and therefore playing a key role in ecosystem structure and function. The aim of this work was to inves-tigate the possible pathways through which glyphosate application affects AMF spores viability and rootcolonization in grassland communities. Our hypothesis is that glyphosate application can damage AMFdirectly (through contact with spores and external hyphae) or indirectly through the changes it generateson host plants. The experiment had a factorial array with three factors: (1) plant species, at two levels(Paspalum dilatatum and Lotus tenuis), (2) doses of glyphosate, at three levels (0 l ha−1, 0.8 l ha−1and 3 l ha−1), and (3) application site, at two levels: soil (direct pathway) and plant foliage (indirect pathway).Spore viability was reduced even under the lowest glyphosate rate, but only when it was applied on thesoil. Total root colonization for both species was similarly decreased when glyphosate was applied toplant foliage or on soil, with no difference between 0.8 and 3 l ha−1. The number of arbuscules was 20%lower when glyphosate was applied on plant foliage, than when it was applied on the soil. Our findingsillustrate that glyphosate application negatively affects AMF functionality in grasslands, due to differentcauses depending on the herbicide application site. While, under field conditions, the occurrence of directand/or indirect pathways will depend on the plant cover at the time of glyphosate application, the con-sequences of this practice on the plant community structure will vary with the mycorrhizal dependenceof the species composition regardless of the pathway involved.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4187
Druille, Magdalena; Omacini, Marina; Golluscio, Rodolfo; Cabello, Marta Noemi; Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application; Elsevier; Applied Soil Ecology; 72; 6-2013; 143-149
0929-1393
url http://hdl.handle.net/11336/4187
identifier_str_mv Druille, Magdalena; Omacini, Marina; Golluscio, Rodolfo; Cabello, Marta Noemi; Arbuscular mycorrhizal fungi are directly and indirectly affected byglyphosate application; Elsevier; Applied Soil Ecology; 72; 6-2013; 143-149
0929-1393
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2013.06.011
info:eu-repo/semantics/altIdentifier/issn/0929-1393
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139313001868
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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