Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity

Autores
Soria, Elio Andres; Quiroga, Patricia L.; Albrecht, Claudia; Ramos Elizagaray, Sabina I.; Cantero, Juan J.; Bongiovanni, Guillermina Azucena
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae)(LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely induced nitrite formation in T- Activated cell cultures (P < 0.0001). Water extraction released a fraction lacking nitrite inducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The infusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG- 1i at 100g/mL), which protected them against in vitro As-induced lymphotoxicity (P < 0.0001). This infusive LG phytoextract contained 10.23 ± 0.43mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound was 0.723 mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was assessed. It contained one-third of saturated fatty acids, one-third of 9, followed by 6 (∼24%) and 3 (∼4%), and scarce 7. Summing up, L. grisebachiiwas a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This supports its phytomedical use and research in order to reduce As-related dysfunctions
Fil: Soria, Elio Andres. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Quiroga, Patricia L.. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Albrecht, Claudia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Ramos Elizagaray, Sabina I.. Consejo Interuniversitario Nacional; Argentina
Fil: Cantero, Juan J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Bongiovanni, Guillermina Azucena. Universidad Nacional del Comahue. Facultad de Ciencis Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Materia
LANTANA GRISEBACHII
LYMPHOPROTECTIVE ACTIVITY
ARSENIC TOXICITY
ANTIOXIDANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11581
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11581
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic ToxicitySoria, Elio AndresQuiroga, Patricia L.Albrecht, ClaudiaRamos Elizagaray, Sabina I.Cantero, Juan J.Bongiovanni, Guillermina AzucenaLANTANA GRISEBACHIILYMPHOPROTECTIVE ACTIVITYARSENIC TOXICITYANTIOXIDANTShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae)(LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely induced nitrite formation in T- Activated cell cultures (P < 0.0001). Water extraction released a fraction lacking nitrite inducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The infusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG- 1i at 100g/mL), which protected them against in vitro As-induced lymphotoxicity (P < 0.0001). This infusive LG phytoextract contained 10.23 ± 0.43mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound was 0.723 mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was assessed. It contained one-third of saturated fatty acids, one-third of 9, followed by 6 (∼24%) and 3 (∼4%), and scarce 7. Summing up, L. grisebachiiwas a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This supports its phytomedical use and research in order to reduce As-related dysfunctionsFil: Soria, Elio Andres. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Quiroga, Patricia L.. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Albrecht, Claudia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Ramos Elizagaray, Sabina I.. Consejo Interuniversitario Nacional; ArgentinaFil: Cantero, Juan J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Bongiovanni, Guillermina Azucena. Universidad Nacional del Comahue. Facultad de Ciencis Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; ArgentinaHindawi Publishing Corporation2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11581Soria, Elio Andres; Quiroga, Patricia L.; Albrecht, Claudia; Ramos Elizagaray, Sabina I.; Cantero, Juan J.; et al.; Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity; Hindawi Publishing Corporation; Advances in Pharmacological Sciences; 6-2014; 1-7; 4167611687-6334enginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/aps/2014/416761/info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1155/2014/416761info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:39:40Zoai:ri.conicet.gov.ar:11336/11581instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:39:40.99CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
title Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
spellingShingle Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
Soria, Elio Andres
LANTANA GRISEBACHII
LYMPHOPROTECTIVE ACTIVITY
ARSENIC TOXICITY
ANTIOXIDANTS
title_short Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
title_full Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
title_fullStr Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
title_full_unstemmed Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
title_sort Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity
dc.creator.none.fl_str_mv Soria, Elio Andres
Quiroga, Patricia L.
Albrecht, Claudia
Ramos Elizagaray, Sabina I.
Cantero, Juan J.
Bongiovanni, Guillermina Azucena
author Soria, Elio Andres
author_facet Soria, Elio Andres
Quiroga, Patricia L.
Albrecht, Claudia
Ramos Elizagaray, Sabina I.
Cantero, Juan J.
Bongiovanni, Guillermina Azucena
author_role author
author2 Quiroga, Patricia L.
Albrecht, Claudia
Ramos Elizagaray, Sabina I.
Cantero, Juan J.
Bongiovanni, Guillermina Azucena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv LANTANA GRISEBACHII
LYMPHOPROTECTIVE ACTIVITY
ARSENIC TOXICITY
ANTIOXIDANTS
topic LANTANA GRISEBACHII
LYMPHOPROTECTIVE ACTIVITY
ARSENIC TOXICITY
ANTIOXIDANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae)(LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely induced nitrite formation in T- Activated cell cultures (P < 0.0001). Water extraction released a fraction lacking nitrite inducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The infusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG- 1i at 100g/mL), which protected them against in vitro As-induced lymphotoxicity (P < 0.0001). This infusive LG phytoextract contained 10.23 ± 0.43mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound was 0.723 mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was assessed. It contained one-third of saturated fatty acids, one-third of 9, followed by 6 (∼24%) and 3 (∼4%), and scarce 7. Summing up, L. grisebachiiwas a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This supports its phytomedical use and research in order to reduce As-related dysfunctions
Fil: Soria, Elio Andres. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Quiroga, Patricia L.. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Albrecht, Claudia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Biologia Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Ramos Elizagaray, Sabina I.. Consejo Interuniversitario Nacional; Argentina
Fil: Cantero, Juan J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Bongiovanni, Guillermina Azucena. Universidad Nacional del Comahue. Facultad de Ciencis Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
description Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae)(LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely induced nitrite formation in T- Activated cell cultures (P < 0.0001). Water extraction released a fraction lacking nitrite inducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The infusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG- 1i at 100g/mL), which protected them against in vitro As-induced lymphotoxicity (P < 0.0001). This infusive LG phytoextract contained 10.23 ± 0.43mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound was 0.723 mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was assessed. It contained one-third of saturated fatty acids, one-third of 9, followed by 6 (∼24%) and 3 (∼4%), and scarce 7. Summing up, L. grisebachiiwas a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This supports its phytomedical use and research in order to reduce As-related dysfunctions
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11581
Soria, Elio Andres; Quiroga, Patricia L.; Albrecht, Claudia; Ramos Elizagaray, Sabina I.; Cantero, Juan J.; et al.; Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity; Hindawi Publishing Corporation; Advances in Pharmacological Sciences; 6-2014; 1-7; 416761
1687-6334
url http://hdl.handle.net/11336/11581
identifier_str_mv Soria, Elio Andres; Quiroga, Patricia L.; Albrecht, Claudia; Ramos Elizagaray, Sabina I.; Cantero, Juan J.; et al.; Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity; Hindawi Publishing Corporation; Advances in Pharmacological Sciences; 6-2014; 1-7; 416761
1687-6334
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/aps/2014/416761/
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1155/2014/416761
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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