Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wi...

Autores
Merlo, Julieta; Cutrera, Ana Paula; Zenuto, Roxana Rita
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The immunological variation in wild populations and its relation to life-history traits has recently become a central topic in the field of evolutionary biology, considering the critical contribution of immunity to an individual’s fitness. A common technique used by ecologists to estimate immunocompetence in wild populations is the phytohemagglutinin (PHA) – skin test. In this test, the degree of local swelling triggered by PHA is usually considered an estimate of T-lymphocyte activity, although there is an ongoing debate regarding this interpretation. Here, we coupled the PHA–skin test with a histological analysis to examine the temporal development of the cell-mediated response in the subterranean rodent Talas tuco-tuco (Ctenomys talarum Thomas, 1898). The inflammation response involved lymphocytes, neutrophils, eosinophils, and macrophages at the site of injection, achieving an increase of total leukocytes from 12 to 48 h after injection. However, the abundance of any of the leukocytes observed did not correlate with the degree of swelling at any time studied, suggesting that caution should be taken when interpreting the results of the PHA-induced swelling response. Particularly, the magnitude of macroscopic swelling should not be considered a priori as indicative of T-lymphocyte activity in wild-caught rodents. Our results highlight the importance of avoiding oversimplified approaches to measuring immunocompetence.
Fil: Merlo, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Cutrera, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Zenuto, Roxana Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
Cellular Immune Response
Ctenomys Talarum
Histological Analysis
Subterranean Rodents
Talas Tuco-Tuco
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34463
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodentsMerlo, JulietaCutrera, Ana PaulaZenuto, Roxana RitaCellular Immune ResponseCtenomys TalarumHistological AnalysisSubterranean RodentsTalas Tuco-Tucohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The immunological variation in wild populations and its relation to life-history traits has recently become a central topic in the field of evolutionary biology, considering the critical contribution of immunity to an individual’s fitness. A common technique used by ecologists to estimate immunocompetence in wild populations is the phytohemagglutinin (PHA) – skin test. In this test, the degree of local swelling triggered by PHA is usually considered an estimate of T-lymphocyte activity, although there is an ongoing debate regarding this interpretation. Here, we coupled the PHA–skin test with a histological analysis to examine the temporal development of the cell-mediated response in the subterranean rodent Talas tuco-tuco (Ctenomys talarum Thomas, 1898). The inflammation response involved lymphocytes, neutrophils, eosinophils, and macrophages at the site of injection, achieving an increase of total leukocytes from 12 to 48 h after injection. However, the abundance of any of the leukocytes observed did not correlate with the degree of swelling at any time studied, suggesting that caution should be taken when interpreting the results of the PHA-induced swelling response. Particularly, the magnitude of macroscopic swelling should not be considered a priori as indicative of T-lymphocyte activity in wild-caught rodents. Our results highlight the importance of avoiding oversimplified approaches to measuring immunocompetence.Fil: Merlo, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Cutrera, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Zenuto, Roxana Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaNational Research Council Canada-NRC Research Press2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34463Merlo, Julieta; Cutrera, Ana Paula; Zenuto, Roxana Rita; Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 92; 8; 5-2014; 689-6970008-4301CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1139/cjz-2013-0306info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/cjz-2013-0306#.WmjeX66Wbmginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:40Zoai:ri.conicet.gov.ar:11336/34463instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:40.857CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents
title Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents
spellingShingle Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents
Merlo, Julieta
Cellular Immune Response
Ctenomys Talarum
Histological Analysis
Subterranean Rodents
Talas Tuco-Tuco
title_short Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents
title_full Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents
title_fullStr Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents
title_full_unstemmed Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents
title_sort Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents
dc.creator.none.fl_str_mv Merlo, Julieta
Cutrera, Ana Paula
Zenuto, Roxana Rita
author Merlo, Julieta
author_facet Merlo, Julieta
Cutrera, Ana Paula
Zenuto, Roxana Rita
author_role author
author2 Cutrera, Ana Paula
Zenuto, Roxana Rita
author2_role author
author
dc.subject.none.fl_str_mv Cellular Immune Response
Ctenomys Talarum
Histological Analysis
Subterranean Rodents
Talas Tuco-Tuco
topic Cellular Immune Response
Ctenomys Talarum
Histological Analysis
Subterranean Rodents
Talas Tuco-Tuco
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The immunological variation in wild populations and its relation to life-history traits has recently become a central topic in the field of evolutionary biology, considering the critical contribution of immunity to an individual’s fitness. A common technique used by ecologists to estimate immunocompetence in wild populations is the phytohemagglutinin (PHA) – skin test. In this test, the degree of local swelling triggered by PHA is usually considered an estimate of T-lymphocyte activity, although there is an ongoing debate regarding this interpretation. Here, we coupled the PHA–skin test with a histological analysis to examine the temporal development of the cell-mediated response in the subterranean rodent Talas tuco-tuco (Ctenomys talarum Thomas, 1898). The inflammation response involved lymphocytes, neutrophils, eosinophils, and macrophages at the site of injection, achieving an increase of total leukocytes from 12 to 48 h after injection. However, the abundance of any of the leukocytes observed did not correlate with the degree of swelling at any time studied, suggesting that caution should be taken when interpreting the results of the PHA-induced swelling response. Particularly, the magnitude of macroscopic swelling should not be considered a priori as indicative of T-lymphocyte activity in wild-caught rodents. Our results highlight the importance of avoiding oversimplified approaches to measuring immunocompetence.
Fil: Merlo, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Cutrera, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Zenuto, Roxana Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description The immunological variation in wild populations and its relation to life-history traits has recently become a central topic in the field of evolutionary biology, considering the critical contribution of immunity to an individual’s fitness. A common technique used by ecologists to estimate immunocompetence in wild populations is the phytohemagglutinin (PHA) – skin test. In this test, the degree of local swelling triggered by PHA is usually considered an estimate of T-lymphocyte activity, although there is an ongoing debate regarding this interpretation. Here, we coupled the PHA–skin test with a histological analysis to examine the temporal development of the cell-mediated response in the subterranean rodent Talas tuco-tuco (Ctenomys talarum Thomas, 1898). The inflammation response involved lymphocytes, neutrophils, eosinophils, and macrophages at the site of injection, achieving an increase of total leukocytes from 12 to 48 h after injection. However, the abundance of any of the leukocytes observed did not correlate with the degree of swelling at any time studied, suggesting that caution should be taken when interpreting the results of the PHA-induced swelling response. Particularly, the magnitude of macroscopic swelling should not be considered a priori as indicative of T-lymphocyte activity in wild-caught rodents. Our results highlight the importance of avoiding oversimplified approaches to measuring immunocompetence.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34463
Merlo, Julieta; Cutrera, Ana Paula; Zenuto, Roxana Rita; Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 92; 8; 5-2014; 689-697
0008-4301
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34463
identifier_str_mv Merlo, Julieta; Cutrera, Ana Paula; Zenuto, Roxana Rita; Inflammation in response to phytohemagglutinin injection in the Talas tuco-tuco (Ctenomys talarum): implications for the estimation of immunocompetence in natural populations of wild rodents; National Research Council Canada-NRC Research Press; Canadian Journal of Zoology; 92; 8; 5-2014; 689-697
0008-4301
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1139/cjz-2013-0306
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/cjz-2013-0306#.WmjeX66Wbmg
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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