Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction

Autores
Brandoni, Anabel; Di Giusto, Gisela; Franca, Raffaella; Passamonti, Sabina; Torres, Adriana Monica
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background/Aim: It has been recently demonstrated that acute obstructive jaundice is associated with modifications in the renal expression and function of organic anion transporters such as Oat1, Oat3, Oatp1 and Mrp2. This study examined the expression and function of bilitranslocase in liver and kidney from rats with bile duct ligation (BDL). Methods: Bilitranslocase expression was evaluated in renal homogenates (H), renal basolateral plasma membranes (KBLM) and liver plasma membranes (LPM) by immunoblotting. Bilitranslocase function was studied by measuring the kinetic parameters of electrogenic bromosulfophthalein (BSP) uptake in KBLM and LPM by a spectrophotometric technique. Results: An increased abundance of bilitranslocase in KBLM without modifications in renal H and in LPM from BDL rats was observed compared with Sham rats. BDL rats showed a higher V max for BSP uptake in KBLM. No differences between groups were observed for Michaelis-Menten parameters in LPM. Conclusion: The higher renal expression and function of bilitranslocase in renal basolateral membranes from rats with obstructive cholestasis might also contribute to the dramatic increase in BSP renal excretion observed in this experimental model. This would be another compensation mechanism to overcome the hepatic dysfunction in the elimination of organic anions.
Fil: Brandoni, Anabel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Di Giusto, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Franca, Raffaella. Università degli Studi di Trieste; Italia
Fil: Passamonti, Sabina. Università degli Studi di Trieste; Italia
Fil: Torres, Adriana Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; Argentina
Materia
CHOLESTASIS
BILITRANSLOCASE
ACUTE JAUNDICE
BILE DUCT OBSTRUCTION
ORGANIC ANION TRANSPORTERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242066
Acceder
id CONICETDig_614f076ee64b42354bc7e4b43b7c01a9
oai_identifier_str oai:ri.conicet.gov.ar:11336/242066
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary ObstructionBrandoni, AnabelDi Giusto, GiselaFranca, RaffaellaPassamonti, SabinaTorres, Adriana MonicaCHOLESTASISBILITRANSLOCASEACUTE JAUNDICEBILE DUCT OBSTRUCTIONORGANIC ANION TRANSPORTERShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background/Aim: It has been recently demonstrated that acute obstructive jaundice is associated with modifications in the renal expression and function of organic anion transporters such as Oat1, Oat3, Oatp1 and Mrp2. This study examined the expression and function of bilitranslocase in liver and kidney from rats with bile duct ligation (BDL). Methods: Bilitranslocase expression was evaluated in renal homogenates (H), renal basolateral plasma membranes (KBLM) and liver plasma membranes (LPM) by immunoblotting. Bilitranslocase function was studied by measuring the kinetic parameters of electrogenic bromosulfophthalein (BSP) uptake in KBLM and LPM by a spectrophotometric technique. Results: An increased abundance of bilitranslocase in KBLM without modifications in renal H and in LPM from BDL rats was observed compared with Sham rats. BDL rats showed a higher V max for BSP uptake in KBLM. No differences between groups were observed for Michaelis-Menten parameters in LPM. Conclusion: The higher renal expression and function of bilitranslocase in renal basolateral membranes from rats with obstructive cholestasis might also contribute to the dramatic increase in BSP renal excretion observed in this experimental model. This would be another compensation mechanism to overcome the hepatic dysfunction in the elimination of organic anions.Fil: Brandoni, Anabel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Di Giusto, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Franca, Raffaella. Università degli Studi di Trieste; ItaliaFil: Passamonti, Sabina. Università degli Studi di Trieste; ItaliaFil: Torres, Adriana Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; ArgentinaKarger2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242066Brandoni, Anabel; Di Giusto, Gisela; Franca, Raffaella; Passamonti, Sabina; Torres, Adriana Monica; Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction; Karger; Nephron Physiology; 114; 4; 1-2010; p35-p401660-2137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000276588info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:11Zoai:ri.conicet.gov.ar:11336/242066instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:11.342CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction
title Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction
spellingShingle Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction
Brandoni, Anabel
CHOLESTASIS
BILITRANSLOCASE
ACUTE JAUNDICE
BILE DUCT OBSTRUCTION
ORGANIC ANION TRANSPORTERS
title_short Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction
title_full Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction
title_fullStr Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction
title_full_unstemmed Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction
title_sort Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction
dc.creator.none.fl_str_mv Brandoni, Anabel
Di Giusto, Gisela
Franca, Raffaella
Passamonti, Sabina
Torres, Adriana Monica
author Brandoni, Anabel
author_facet Brandoni, Anabel
Di Giusto, Gisela
Franca, Raffaella
Passamonti, Sabina
Torres, Adriana Monica
author_role author
author2 Di Giusto, Gisela
Franca, Raffaella
Passamonti, Sabina
Torres, Adriana Monica
author2_role author
author
author
author
dc.subject.none.fl_str_mv CHOLESTASIS
BILITRANSLOCASE
ACUTE JAUNDICE
BILE DUCT OBSTRUCTION
ORGANIC ANION TRANSPORTERS
topic CHOLESTASIS
BILITRANSLOCASE
ACUTE JAUNDICE
BILE DUCT OBSTRUCTION
ORGANIC ANION TRANSPORTERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background/Aim: It has been recently demonstrated that acute obstructive jaundice is associated with modifications in the renal expression and function of organic anion transporters such as Oat1, Oat3, Oatp1 and Mrp2. This study examined the expression and function of bilitranslocase in liver and kidney from rats with bile duct ligation (BDL). Methods: Bilitranslocase expression was evaluated in renal homogenates (H), renal basolateral plasma membranes (KBLM) and liver plasma membranes (LPM) by immunoblotting. Bilitranslocase function was studied by measuring the kinetic parameters of electrogenic bromosulfophthalein (BSP) uptake in KBLM and LPM by a spectrophotometric technique. Results: An increased abundance of bilitranslocase in KBLM without modifications in renal H and in LPM from BDL rats was observed compared with Sham rats. BDL rats showed a higher V max for BSP uptake in KBLM. No differences between groups were observed for Michaelis-Menten parameters in LPM. Conclusion: The higher renal expression and function of bilitranslocase in renal basolateral membranes from rats with obstructive cholestasis might also contribute to the dramatic increase in BSP renal excretion observed in this experimental model. This would be another compensation mechanism to overcome the hepatic dysfunction in the elimination of organic anions.
Fil: Brandoni, Anabel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Di Giusto, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Franca, Raffaella. Università degli Studi di Trieste; Italia
Fil: Passamonti, Sabina. Università degli Studi di Trieste; Italia
Fil: Torres, Adriana Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Ciencias Fisiológicas. Area Farmacología; Argentina
description Background/Aim: It has been recently demonstrated that acute obstructive jaundice is associated with modifications in the renal expression and function of organic anion transporters such as Oat1, Oat3, Oatp1 and Mrp2. This study examined the expression and function of bilitranslocase in liver and kidney from rats with bile duct ligation (BDL). Methods: Bilitranslocase expression was evaluated in renal homogenates (H), renal basolateral plasma membranes (KBLM) and liver plasma membranes (LPM) by immunoblotting. Bilitranslocase function was studied by measuring the kinetic parameters of electrogenic bromosulfophthalein (BSP) uptake in KBLM and LPM by a spectrophotometric technique. Results: An increased abundance of bilitranslocase in KBLM without modifications in renal H and in LPM from BDL rats was observed compared with Sham rats. BDL rats showed a higher V max for BSP uptake in KBLM. No differences between groups were observed for Michaelis-Menten parameters in LPM. Conclusion: The higher renal expression and function of bilitranslocase in renal basolateral membranes from rats with obstructive cholestasis might also contribute to the dramatic increase in BSP renal excretion observed in this experimental model. This would be another compensation mechanism to overcome the hepatic dysfunction in the elimination of organic anions.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242066
Brandoni, Anabel; Di Giusto, Gisela; Franca, Raffaella; Passamonti, Sabina; Torres, Adriana Monica; Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction; Karger; Nephron Physiology; 114; 4; 1-2010; p35-p40
1660-2137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242066
identifier_str_mv Brandoni, Anabel; Di Giusto, Gisela; Franca, Raffaella; Passamonti, Sabina; Torres, Adriana Monica; Expression of Kidney and Liver Bilitranslocase in Response to Acute Biliary Obstruction; Karger; Nephron Physiology; 114; 4; 1-2010; p35-p40
1660-2137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000276588
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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