Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice

Autores
Soriano, Delia Beatriz; Vacotto, Marina; Brusco, Herminia Alicia; Caltana, Laura Romina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cannabinoid receptor type 1 (CB1R) modulates synaptic activity and is widely distributed in brain areas such as the hippocampus, cerebellum, cerebral cortex, and striatum, among others. CB1R is involved in processes such as memory, learning, motor coordination, and mood. Genetic deletion of CB1R causes behavioral alterations. In this work, we evaluated neuronal morphology and synaptic structure in the hippocampus of adult male CB1R knockout mice (CB1R−/−). Morphological changes in the CB1R−/− hippocampus evidenced a decrease in the expression of cytoskeletal proteins neurofilaments 160 KDa, neurofilaments 200 KDa, and microtubule-associated protein 2. CA1 neurons showed decreased arborization and changes in synaptic structure such as lower thickness of postsynaptic density and a reduction in synaptophysin levels. Results obtained in the present work provide evidence of the participation of CB1R in the establishment of neuronal structure and networks that could have an important role in neuronal plasticity. In addition, these changes observed in CB1R−/− could be correlated with behavioral alterations reported.
Fil: Soriano, Delia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Vacotto, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Materia
CANNABINOID RECEPTOR TYPE 1
CANNABINOID SYSTEM
NEURONAL PLASTICITY
RRID:AB_10015282
RRID:AB_143162
RRID:AB_2340770
RRID:AB_476738
RRID:AB_477193
RRID:AB_477257
RRID:AB_477261
RRID:AB_477523
SYNAPSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148369
Acceder
id CONICETDig_6093b5d4360203ac62c3ab378abc1538
oai_identifier_str oai:ri.conicet.gov.ar:11336/148369
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout miceSoriano, Delia BeatrizVacotto, MarinaBrusco, Herminia AliciaCaltana, Laura RominaCANNABINOID RECEPTOR TYPE 1CANNABINOID SYSTEMNEURONAL PLASTICITYRRID:AB_10015282RRID:AB_143162RRID:AB_2340770RRID:AB_476738RRID:AB_477193RRID:AB_477257RRID:AB_477261RRID:AB_477523SYNAPSEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Cannabinoid receptor type 1 (CB1R) modulates synaptic activity and is widely distributed in brain areas such as the hippocampus, cerebellum, cerebral cortex, and striatum, among others. CB1R is involved in processes such as memory, learning, motor coordination, and mood. Genetic deletion of CB1R causes behavioral alterations. In this work, we evaluated neuronal morphology and synaptic structure in the hippocampus of adult male CB1R knockout mice (CB1R−/−). Morphological changes in the CB1R−/− hippocampus evidenced a decrease in the expression of cytoskeletal proteins neurofilaments 160 KDa, neurofilaments 200 KDa, and microtubule-associated protein 2. CA1 neurons showed decreased arborization and changes in synaptic structure such as lower thickness of postsynaptic density and a reduction in synaptophysin levels. Results obtained in the present work provide evidence of the participation of CB1R in the establishment of neuronal structure and networks that could have an important role in neuronal plasticity. In addition, these changes observed in CB1R−/− could be correlated with behavioral alterations reported.Fil: Soriano, Delia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Vacotto, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaWiley-liss, div John Wiley & Sons Inc.2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148369Soriano, Delia Beatriz; Vacotto, Marina; Brusco, Herminia Alicia; Caltana, Laura Romina; Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice; Wiley-liss, div John Wiley & Sons Inc.; Journal of Neuroscience Research; 98; 11; 7-2020; 2245-22620360-4012CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jnr.24694info:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.24694info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:19Zoai:ri.conicet.gov.ar:11336/148369instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:19.899CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice
title Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice
spellingShingle Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice
Soriano, Delia Beatriz
CANNABINOID RECEPTOR TYPE 1
CANNABINOID SYSTEM
NEURONAL PLASTICITY
RRID:AB_10015282
RRID:AB_143162
RRID:AB_2340770
RRID:AB_476738
RRID:AB_477193
RRID:AB_477257
RRID:AB_477261
RRID:AB_477523
SYNAPSE
title_short Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice
title_full Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice
title_fullStr Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice
title_full_unstemmed Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice
title_sort Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice
dc.creator.none.fl_str_mv Soriano, Delia Beatriz
Vacotto, Marina
Brusco, Herminia Alicia
Caltana, Laura Romina
author Soriano, Delia Beatriz
author_facet Soriano, Delia Beatriz
Vacotto, Marina
Brusco, Herminia Alicia
Caltana, Laura Romina
author_role author
author2 Vacotto, Marina
Brusco, Herminia Alicia
Caltana, Laura Romina
author2_role author
author
author
dc.subject.none.fl_str_mv CANNABINOID RECEPTOR TYPE 1
CANNABINOID SYSTEM
NEURONAL PLASTICITY
RRID:AB_10015282
RRID:AB_143162
RRID:AB_2340770
RRID:AB_476738
RRID:AB_477193
RRID:AB_477257
RRID:AB_477261
RRID:AB_477523
SYNAPSE
topic CANNABINOID RECEPTOR TYPE 1
CANNABINOID SYSTEM
NEURONAL PLASTICITY
RRID:AB_10015282
RRID:AB_143162
RRID:AB_2340770
RRID:AB_476738
RRID:AB_477193
RRID:AB_477257
RRID:AB_477261
RRID:AB_477523
SYNAPSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cannabinoid receptor type 1 (CB1R) modulates synaptic activity and is widely distributed in brain areas such as the hippocampus, cerebellum, cerebral cortex, and striatum, among others. CB1R is involved in processes such as memory, learning, motor coordination, and mood. Genetic deletion of CB1R causes behavioral alterations. In this work, we evaluated neuronal morphology and synaptic structure in the hippocampus of adult male CB1R knockout mice (CB1R−/−). Morphological changes in the CB1R−/− hippocampus evidenced a decrease in the expression of cytoskeletal proteins neurofilaments 160 KDa, neurofilaments 200 KDa, and microtubule-associated protein 2. CA1 neurons showed decreased arborization and changes in synaptic structure such as lower thickness of postsynaptic density and a reduction in synaptophysin levels. Results obtained in the present work provide evidence of the participation of CB1R in the establishment of neuronal structure and networks that could have an important role in neuronal plasticity. In addition, these changes observed in CB1R−/− could be correlated with behavioral alterations reported.
Fil: Soriano, Delia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Vacotto, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
description Cannabinoid receptor type 1 (CB1R) modulates synaptic activity and is widely distributed in brain areas such as the hippocampus, cerebellum, cerebral cortex, and striatum, among others. CB1R is involved in processes such as memory, learning, motor coordination, and mood. Genetic deletion of CB1R causes behavioral alterations. In this work, we evaluated neuronal morphology and synaptic structure in the hippocampus of adult male CB1R knockout mice (CB1R−/−). Morphological changes in the CB1R−/− hippocampus evidenced a decrease in the expression of cytoskeletal proteins neurofilaments 160 KDa, neurofilaments 200 KDa, and microtubule-associated protein 2. CA1 neurons showed decreased arborization and changes in synaptic structure such as lower thickness of postsynaptic density and a reduction in synaptophysin levels. Results obtained in the present work provide evidence of the participation of CB1R in the establishment of neuronal structure and networks that could have an important role in neuronal plasticity. In addition, these changes observed in CB1R−/− could be correlated with behavioral alterations reported.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148369
Soriano, Delia Beatriz; Vacotto, Marina; Brusco, Herminia Alicia; Caltana, Laura Romina; Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice; Wiley-liss, div John Wiley & Sons Inc.; Journal of Neuroscience Research; 98; 11; 7-2020; 2245-2262
0360-4012
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148369
identifier_str_mv Soriano, Delia Beatriz; Vacotto, Marina; Brusco, Herminia Alicia; Caltana, Laura Romina; Neuronal and synaptic morphological alterations in the hippocampus of cannabinoid receptor type 1 knockout mice; Wiley-liss, div John Wiley & Sons Inc.; Journal of Neuroscience Research; 98; 11; 7-2020; 2245-2262
0360-4012
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jnr.24694
info:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.24694
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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