Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina

Autores
Salazar, S. M.; Moschen, Sebastián Nicolás; Arias, M. E.; Furio, Ramiro Nicolás; Castagnaro, Atilio Pedro; Diaz Ricci, Juan Carlos
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: Anthracnose is a disease caused by Colletotrichum species and is a significant disease in the strawberry crop. This study aims to identify at the level of species Colletotrichum spp. isolates collected from symptomatic strawberry plants in Tucumán, Argentina’s second most important strawberry production area.Methods: 45 isolates of Colletotrichum were collected and characterized. Morphological characterization was conducted by analyzing fungal cultural characteristics, the growth rate in potato dextrose agar plates, conidial morphology and size, and sexual state. Phytopathological characterization was carried out by plant-pathogen interactions under controlled conditions in infection chambers. Molecular characterization was performed by polymerase chain reaction analysis of regions of amplified mitochondrial small rRNA genes. Results: Preliminary morphological characterization led to three distinctive groups, based on conidial shape and size, and colony type, colour, and growth, whereas the phytopathological characterization, led to two distinct groups based on the severity of symptoms, virulence, and host specificity. Molecular analyses confirmed the identity of three isolates representing each of the microbiological groups (i.e., isolate F7 of Colletotrichum acutatum (C. acutatum), isolate L9 of C. acutatum, isolate M11 of C. acutatum), demonstrating that they correspond to C. acutatum species. Based on the analysis of ribosomal DNA internal transcribed spacers sequences, and using specific microsatellites, we found a high genetic variability among all C. acutatum sequences. Implications of these results, as well as biological products commercially available and research advancing the field of biological control agents, were also discussed. Conclusion: This is the latest study to report with confidence that the species of Colletotrichum present in Tucumán, Argentina corresponds to C. acutatum, and such species are genetically diverse.
Fil: Salazar, S. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Moschen, Sebastián Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Arias, M. E.. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Furio, Ramiro Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
COLLETOTRICHUM
ANTHRACNOSE
STRAWBERRY
FRAGARIA X ANANASSA
BIOLOGICAL CONTROL AGENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217919
Acceder
id CONICETDig_606b3610cd81e3d0594e3b874823dee5
oai_identifier_str oai:ri.conicet.gov.ar:11336/217919
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, ArgentinaSalazar, S. M.Moschen, Sebastián NicolásArias, M. E.Furio, Ramiro NicolásCastagnaro, Atilio PedroDiaz Ricci, Juan CarlosCOLLETOTRICHUMANTHRACNOSESTRAWBERRYFRAGARIA X ANANASSABIOLOGICAL CONTROL AGENTShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Objective: Anthracnose is a disease caused by Colletotrichum species and is a significant disease in the strawberry crop. This study aims to identify at the level of species Colletotrichum spp. isolates collected from symptomatic strawberry plants in Tucumán, Argentina’s second most important strawberry production area.Methods: 45 isolates of Colletotrichum were collected and characterized. Morphological characterization was conducted by analyzing fungal cultural characteristics, the growth rate in potato dextrose agar plates, conidial morphology and size, and sexual state. Phytopathological characterization was carried out by plant-pathogen interactions under controlled conditions in infection chambers. Molecular characterization was performed by polymerase chain reaction analysis of regions of amplified mitochondrial small rRNA genes. Results: Preliminary morphological characterization led to three distinctive groups, based on conidial shape and size, and colony type, colour, and growth, whereas the phytopathological characterization, led to two distinct groups based on the severity of symptoms, virulence, and host specificity. Molecular analyses confirmed the identity of three isolates representing each of the microbiological groups (i.e., isolate F7 of Colletotrichum acutatum (C. acutatum), isolate L9 of C. acutatum, isolate M11 of C. acutatum), demonstrating that they correspond to C. acutatum species. Based on the analysis of ribosomal DNA internal transcribed spacers sequences, and using specific microsatellites, we found a high genetic variability among all C. acutatum sequences. Implications of these results, as well as biological products commercially available and research advancing the field of biological control agents, were also discussed. Conclusion: This is the latest study to report with confidence that the species of Colletotrichum present in Tucumán, Argentina corresponds to C. acutatum, and such species are genetically diverse.Fil: Salazar, S. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Moschen, Sebastián Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Arias, M. E.. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Furio, Ramiro Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaInnovation Forever Publishing Group Limited2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217919Salazar, S. M.; Moschen, Sebastián Nicolás; Arias, M. E.; Furio, Ramiro Nicolás; Castagnaro, Atilio Pedro; et al.; Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina; Innovation Forever Publishing Group Limited; Journal of Modern Agriculture and Biotechnology; 11-2022; 1-152788-810XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.53964/jmab.2022026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:36Zoai:ri.conicet.gov.ar:11336/217919instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:36.853CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina
title Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina
spellingShingle Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina
Salazar, S. M.
COLLETOTRICHUM
ANTHRACNOSE
STRAWBERRY
FRAGARIA X ANANASSA
BIOLOGICAL CONTROL AGENTS
title_short Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina
title_full Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina
title_fullStr Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina
title_full_unstemmed Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina
title_sort Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina
dc.creator.none.fl_str_mv Salazar, S. M.
Moschen, Sebastián Nicolás
Arias, M. E.
Furio, Ramiro Nicolás
Castagnaro, Atilio Pedro
Diaz Ricci, Juan Carlos
author Salazar, S. M.
author_facet Salazar, S. M.
Moschen, Sebastián Nicolás
Arias, M. E.
Furio, Ramiro Nicolás
Castagnaro, Atilio Pedro
Diaz Ricci, Juan Carlos
author_role author
author2 Moschen, Sebastián Nicolás
Arias, M. E.
Furio, Ramiro Nicolás
Castagnaro, Atilio Pedro
Diaz Ricci, Juan Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv COLLETOTRICHUM
ANTHRACNOSE
STRAWBERRY
FRAGARIA X ANANASSA
BIOLOGICAL CONTROL AGENTS
topic COLLETOTRICHUM
ANTHRACNOSE
STRAWBERRY
FRAGARIA X ANANASSA
BIOLOGICAL CONTROL AGENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Objective: Anthracnose is a disease caused by Colletotrichum species and is a significant disease in the strawberry crop. This study aims to identify at the level of species Colletotrichum spp. isolates collected from symptomatic strawberry plants in Tucumán, Argentina’s second most important strawberry production area.Methods: 45 isolates of Colletotrichum were collected and characterized. Morphological characterization was conducted by analyzing fungal cultural characteristics, the growth rate in potato dextrose agar plates, conidial morphology and size, and sexual state. Phytopathological characterization was carried out by plant-pathogen interactions under controlled conditions in infection chambers. Molecular characterization was performed by polymerase chain reaction analysis of regions of amplified mitochondrial small rRNA genes. Results: Preliminary morphological characterization led to three distinctive groups, based on conidial shape and size, and colony type, colour, and growth, whereas the phytopathological characterization, led to two distinct groups based on the severity of symptoms, virulence, and host specificity. Molecular analyses confirmed the identity of three isolates representing each of the microbiological groups (i.e., isolate F7 of Colletotrichum acutatum (C. acutatum), isolate L9 of C. acutatum, isolate M11 of C. acutatum), demonstrating that they correspond to C. acutatum species. Based on the analysis of ribosomal DNA internal transcribed spacers sequences, and using specific microsatellites, we found a high genetic variability among all C. acutatum sequences. Implications of these results, as well as biological products commercially available and research advancing the field of biological control agents, were also discussed. Conclusion: This is the latest study to report with confidence that the species of Colletotrichum present in Tucumán, Argentina corresponds to C. acutatum, and such species are genetically diverse.
Fil: Salazar, S. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Moschen, Sebastián Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Arias, M. E.. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Furio, Ramiro Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Objective: Anthracnose is a disease caused by Colletotrichum species and is a significant disease in the strawberry crop. This study aims to identify at the level of species Colletotrichum spp. isolates collected from symptomatic strawberry plants in Tucumán, Argentina’s second most important strawberry production area.Methods: 45 isolates of Colletotrichum were collected and characterized. Morphological characterization was conducted by analyzing fungal cultural characteristics, the growth rate in potato dextrose agar plates, conidial morphology and size, and sexual state. Phytopathological characterization was carried out by plant-pathogen interactions under controlled conditions in infection chambers. Molecular characterization was performed by polymerase chain reaction analysis of regions of amplified mitochondrial small rRNA genes. Results: Preliminary morphological characterization led to three distinctive groups, based on conidial shape and size, and colony type, colour, and growth, whereas the phytopathological characterization, led to two distinct groups based on the severity of symptoms, virulence, and host specificity. Molecular analyses confirmed the identity of three isolates representing each of the microbiological groups (i.e., isolate F7 of Colletotrichum acutatum (C. acutatum), isolate L9 of C. acutatum, isolate M11 of C. acutatum), demonstrating that they correspond to C. acutatum species. Based on the analysis of ribosomal DNA internal transcribed spacers sequences, and using specific microsatellites, we found a high genetic variability among all C. acutatum sequences. Implications of these results, as well as biological products commercially available and research advancing the field of biological control agents, were also discussed. Conclusion: This is the latest study to report with confidence that the species of Colletotrichum present in Tucumán, Argentina corresponds to C. acutatum, and such species are genetically diverse.
publishDate 2022
dc.date.none.fl_str_mv 2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217919
Salazar, S. M.; Moschen, Sebastián Nicolás; Arias, M. E.; Furio, Ramiro Nicolás; Castagnaro, Atilio Pedro; et al.; Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina; Innovation Forever Publishing Group Limited; Journal of Modern Agriculture and Biotechnology; 11-2022; 1-15
2788-810X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217919
identifier_str_mv Salazar, S. M.; Moschen, Sebastián Nicolás; Arias, M. E.; Furio, Ramiro Nicolás; Castagnaro, Atilio Pedro; et al.; Characterization of Colletotrichum Species as Causal Agents of Anthracnose in Strawberry Productive Areas in Tucumán, Argentina; Innovation Forever Publishing Group Limited; Journal of Modern Agriculture and Biotechnology; 11-2022; 1-15
2788-810X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.53964/jmab.2022026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Innovation Forever Publishing Group Limited
publisher.none.fl_str_mv Innovation Forever Publishing Group Limited
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270051991814144
score 13.13397

Publicaciones similares