Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina

Autores
Tanaro, José Daniel; Galli, Lucía; Lound, Liliana Haydee; Leotta, Gerardo Anibal; Piaggio, Mercedes Carolina; Carbonari, Claudia Carolina; Kinue, Irino; Rivas, Marta
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purposes of this study were to detect non-O157 Shiga toxin-producing Escherichia coli (STEC) in bovine rectums and water in a beef cattle farm in Argentina, and to determine the pathogenic potential of the circulating strains. During the study, 292 rectal swabs from healthy animals and 79 environmental water samples were collected. The rectal swabs and one loop of the Moore swabs, enriched in Escherichia coli broth for 24 h at 37°C, were streaked on MacConkey agar plates and incubated overnight at 37°C. The isolates were characterized by biochemical tests and serotyped. Nonmotile STEC strains were typed for their H-specific (fliC) antigens by polymerase chain reaction (PCR). Isolates were characterized by detection of stx1, stx2, and their variants, eae, ehxA, and saa genes. Macrorestriction fragment analysis by pulsed-field gel electrophoresis (PFGE) was performed using the PulseNet standardized protocol. From 371 samples analyzed, 36.6% of rectal swabs and 34.2% of water samples were non-O157 STEC-positive by PCR, and 110 strains from rectal swabs, but only three from water, were isolated. The strains were grouped into 24 different serotypes, from which, O103:[H2] (n = 12), O136:H12 (n = 8), O178:H19 (n = 8), and O103:NM (n = 5) were most prevalent, representing 29.2% of the isolates. Predominant genotypes were stx1/eae/ehxA (16.8%) and stx2/saa/ehxA (15.9%). PFGE analysis revealed 56 different patterns, with 65 strains grouped in 19 clusters of 100% similarity. Two STEC O124:H19 strains isolated from rectal swabs and water with a 5-month interval harbored the stx1/stx2/saa/ehxA genotype, and showed an indistinguishable PFGE profile. By comparison, some XbaI-PFGE patterns identified in the present study were identical to the profiles of strains isolated from human, food, and animal sources included in the Argentine PulseNet database. By PCR, similar non-O157 detection rates were found in rectal swabs and water. However, the methodology for water samples needs to be improved, since only three strains from the total number of positive samples were recovered.
Fil: Tanaro, José Daniel. Universidad Nacional de Entre Ríos. Facultad de Bromatología; Argentina
Fil: Galli, Lucía. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Lound, Liliana Haydee. Universidad Nacional de Entre Ríos. Facultad de Bromatología; Argentina
Fil: Leotta, Gerardo Anibal. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Piaggio, Mercedes Carolina. Universidad Nacional de Entre Ríos. Facultad de Bromatología; Argentina
Fil: Carbonari, Claudia Carolina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Kinue, Irino. Escola Nacional de Saude Publica Sergio Arouca; Brasil
Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Materia
NON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC)
BOVINE RECTUMS
WATER
POLYMERASE CHAIN REACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156531
Acceder
id CONICETDig_5e30c2489e450a8bce6b92347c3767da
oai_identifier_str oai:ri.conicet.gov.ar:11336/156531
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in ArgentinaTanaro, José DanielGalli, LucíaLound, Liliana HaydeeLeotta, Gerardo AnibalPiaggio, Mercedes CarolinaCarbonari, Claudia CarolinaKinue, IrinoRivas, MartaNON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC)BOVINE RECTUMSWATERPOLYMERASE CHAIN REACTIONhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4The purposes of this study were to detect non-O157 Shiga toxin-producing Escherichia coli (STEC) in bovine rectums and water in a beef cattle farm in Argentina, and to determine the pathogenic potential of the circulating strains. During the study, 292 rectal swabs from healthy animals and 79 environmental water samples were collected. The rectal swabs and one loop of the Moore swabs, enriched in Escherichia coli broth for 24 h at 37°C, were streaked on MacConkey agar plates and incubated overnight at 37°C. The isolates were characterized by biochemical tests and serotyped. Nonmotile STEC strains were typed for their H-specific (fliC) antigens by polymerase chain reaction (PCR). Isolates were characterized by detection of stx1, stx2, and their variants, eae, ehxA, and saa genes. Macrorestriction fragment analysis by pulsed-field gel electrophoresis (PFGE) was performed using the PulseNet standardized protocol. From 371 samples analyzed, 36.6% of rectal swabs and 34.2% of water samples were non-O157 STEC-positive by PCR, and 110 strains from rectal swabs, but only three from water, were isolated. The strains were grouped into 24 different serotypes, from which, O103:[H2] (n = 12), O136:H12 (n = 8), O178:H19 (n = 8), and O103:NM (n = 5) were most prevalent, representing 29.2% of the isolates. Predominant genotypes were stx1/eae/ehxA (16.8%) and stx2/saa/ehxA (15.9%). PFGE analysis revealed 56 different patterns, with 65 strains grouped in 19 clusters of 100% similarity. Two STEC O124:H19 strains isolated from rectal swabs and water with a 5-month interval harbored the stx1/stx2/saa/ehxA genotype, and showed an indistinguishable PFGE profile. By comparison, some XbaI-PFGE patterns identified in the present study were identical to the profiles of strains isolated from human, food, and animal sources included in the Argentine PulseNet database. By PCR, similar non-O157 detection rates were found in rectal swabs and water. However, the methodology for water samples needs to be improved, since only three strains from the total number of positive samples were recovered.Fil: Tanaro, José Daniel. Universidad Nacional de Entre Ríos. Facultad de Bromatología; ArgentinaFil: Galli, Lucía. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Lound, Liliana Haydee. Universidad Nacional de Entre Ríos. Facultad de Bromatología; ArgentinaFil: Leotta, Gerardo Anibal. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Piaggio, Mercedes Carolina. Universidad Nacional de Entre Ríos. Facultad de Bromatología; ArgentinaFil: Carbonari, Claudia Carolina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Kinue, Irino. Escola Nacional de Saude Publica Sergio Arouca; BrasilFil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaMary Ann Liebert2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156531Tanaro, José Daniel; Galli, Lucía; Lound, Liliana Haydee; Leotta, Gerardo Anibal; Piaggio, Mercedes Carolina; et al.; Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina; Mary Ann Liebert; Foodborne Pathogens And Disease; 9; 10; 10-2012; 878-8841535-3141CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/22994915info:eu-repo/semantics/altIdentifier/doi/10.1089/fpd.2012.1182info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:27Zoai:ri.conicet.gov.ar:11336/156531instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:28.136CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina
title Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina
spellingShingle Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina
Tanaro, José Daniel
NON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC)
BOVINE RECTUMS
WATER
POLYMERASE CHAIN REACTION
title_short Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina
title_full Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina
title_fullStr Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina
title_full_unstemmed Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina
title_sort Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina
dc.creator.none.fl_str_mv Tanaro, José Daniel
Galli, Lucía
Lound, Liliana Haydee
Leotta, Gerardo Anibal
Piaggio, Mercedes Carolina
Carbonari, Claudia Carolina
Kinue, Irino
Rivas, Marta
author Tanaro, José Daniel
author_facet Tanaro, José Daniel
Galli, Lucía
Lound, Liliana Haydee
Leotta, Gerardo Anibal
Piaggio, Mercedes Carolina
Carbonari, Claudia Carolina
Kinue, Irino
Rivas, Marta
author_role author
author2 Galli, Lucía
Lound, Liliana Haydee
Leotta, Gerardo Anibal
Piaggio, Mercedes Carolina
Carbonari, Claudia Carolina
Kinue, Irino
Rivas, Marta
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC)
BOVINE RECTUMS
WATER
POLYMERASE CHAIN REACTION
topic NON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC)
BOVINE RECTUMS
WATER
POLYMERASE CHAIN REACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The purposes of this study were to detect non-O157 Shiga toxin-producing Escherichia coli (STEC) in bovine rectums and water in a beef cattle farm in Argentina, and to determine the pathogenic potential of the circulating strains. During the study, 292 rectal swabs from healthy animals and 79 environmental water samples were collected. The rectal swabs and one loop of the Moore swabs, enriched in Escherichia coli broth for 24 h at 37°C, were streaked on MacConkey agar plates and incubated overnight at 37°C. The isolates were characterized by biochemical tests and serotyped. Nonmotile STEC strains were typed for their H-specific (fliC) antigens by polymerase chain reaction (PCR). Isolates were characterized by detection of stx1, stx2, and their variants, eae, ehxA, and saa genes. Macrorestriction fragment analysis by pulsed-field gel electrophoresis (PFGE) was performed using the PulseNet standardized protocol. From 371 samples analyzed, 36.6% of rectal swabs and 34.2% of water samples were non-O157 STEC-positive by PCR, and 110 strains from rectal swabs, but only three from water, were isolated. The strains were grouped into 24 different serotypes, from which, O103:[H2] (n = 12), O136:H12 (n = 8), O178:H19 (n = 8), and O103:NM (n = 5) were most prevalent, representing 29.2% of the isolates. Predominant genotypes were stx1/eae/ehxA (16.8%) and stx2/saa/ehxA (15.9%). PFGE analysis revealed 56 different patterns, with 65 strains grouped in 19 clusters of 100% similarity. Two STEC O124:H19 strains isolated from rectal swabs and water with a 5-month interval harbored the stx1/stx2/saa/ehxA genotype, and showed an indistinguishable PFGE profile. By comparison, some XbaI-PFGE patterns identified in the present study were identical to the profiles of strains isolated from human, food, and animal sources included in the Argentine PulseNet database. By PCR, similar non-O157 detection rates were found in rectal swabs and water. However, the methodology for water samples needs to be improved, since only three strains from the total number of positive samples were recovered.
Fil: Tanaro, José Daniel. Universidad Nacional de Entre Ríos. Facultad de Bromatología; Argentina
Fil: Galli, Lucía. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Lound, Liliana Haydee. Universidad Nacional de Entre Ríos. Facultad de Bromatología; Argentina
Fil: Leotta, Gerardo Anibal. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Piaggio, Mercedes Carolina. Universidad Nacional de Entre Ríos. Facultad de Bromatología; Argentina
Fil: Carbonari, Claudia Carolina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Kinue, Irino. Escola Nacional de Saude Publica Sergio Arouca; Brasil
Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
description The purposes of this study were to detect non-O157 Shiga toxin-producing Escherichia coli (STEC) in bovine rectums and water in a beef cattle farm in Argentina, and to determine the pathogenic potential of the circulating strains. During the study, 292 rectal swabs from healthy animals and 79 environmental water samples were collected. The rectal swabs and one loop of the Moore swabs, enriched in Escherichia coli broth for 24 h at 37°C, were streaked on MacConkey agar plates and incubated overnight at 37°C. The isolates were characterized by biochemical tests and serotyped. Nonmotile STEC strains were typed for their H-specific (fliC) antigens by polymerase chain reaction (PCR). Isolates were characterized by detection of stx1, stx2, and their variants, eae, ehxA, and saa genes. Macrorestriction fragment analysis by pulsed-field gel electrophoresis (PFGE) was performed using the PulseNet standardized protocol. From 371 samples analyzed, 36.6% of rectal swabs and 34.2% of water samples were non-O157 STEC-positive by PCR, and 110 strains from rectal swabs, but only three from water, were isolated. The strains were grouped into 24 different serotypes, from which, O103:[H2] (n = 12), O136:H12 (n = 8), O178:H19 (n = 8), and O103:NM (n = 5) were most prevalent, representing 29.2% of the isolates. Predominant genotypes were stx1/eae/ehxA (16.8%) and stx2/saa/ehxA (15.9%). PFGE analysis revealed 56 different patterns, with 65 strains grouped in 19 clusters of 100% similarity. Two STEC O124:H19 strains isolated from rectal swabs and water with a 5-month interval harbored the stx1/stx2/saa/ehxA genotype, and showed an indistinguishable PFGE profile. By comparison, some XbaI-PFGE patterns identified in the present study were identical to the profiles of strains isolated from human, food, and animal sources included in the Argentine PulseNet database. By PCR, similar non-O157 detection rates were found in rectal swabs and water. However, the methodology for water samples needs to be improved, since only three strains from the total number of positive samples were recovered.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156531
Tanaro, José Daniel; Galli, Lucía; Lound, Liliana Haydee; Leotta, Gerardo Anibal; Piaggio, Mercedes Carolina; et al.; Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina; Mary Ann Liebert; Foodborne Pathogens And Disease; 9; 10; 10-2012; 878-884
1535-3141
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156531
identifier_str_mv Tanaro, José Daniel; Galli, Lucía; Lound, Liliana Haydee; Leotta, Gerardo Anibal; Piaggio, Mercedes Carolina; et al.; Non-O157:H7 Shiga Toxin Producing Escherichia coli in Bovine Rectums and Surface Water Streams on a Beef Cattle Farm in Argentina; Mary Ann Liebert; Foodborne Pathogens And Disease; 9; 10; 10-2012; 878-884
1535-3141
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/22994915
info:eu-repo/semantics/altIdentifier/doi/10.1089/fpd.2012.1182
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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