Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy

Autores
Fricke, Anna Lena; Kihara, Terue C.; Hoppenrath, Mona
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mesoalgae play a key role in shallow coastal ecosystems. Composed of small macroalgae, intermixed with filamentous cyanobacteria and colonial diatoms, these multi-specific, but minute (μm-cm) assemblages form complex three-dimensional structures, providing shelter for different unicellular (e.g. bacteria, diatoms, dinoflagellates) and multicellular (e.g. fishes, invertebrates) organisms. Characterized by a high colonization potential, these primary producers are observed to bloom and overgrow disturbed areas (e.g. damaged coral reefs, urchin barrens), and play a crucial role in terms of invasion and colonizing new habitats. Driven by anthropogenic environmental changes, mesoalgae are receiving considerable attention in current marine research. So far, most studies approach mesoalgae at the functional group level (e.g. turf algae, microphytobenthos), whereas only few studies tackle the importance of species-specific interactions, which play an important role in benthic ecology (e.g. coral-algal competition and disease spreading). To facilitate the study of not only the presence but also the composition and the structure of these habitat formers, we provide a new approach combining inexpensive fixation methodology with modern confocal laser scanning microscopy (CLSM), to study minute macroalgal structures (e.g. germlings, reproductive structures), and investigate their relation to microphytobenthic components (e.g. diatom colonies). Detailed procedures for mounting, staining and imaging phytobenthic communities are provided.
Fil: Fricke, Anna Lena. Zentrum Fur Marine Tropenokologie Bremen; . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz Zentrum für Marine Tropenforschung; Alemania
Fil: Kihara, Terue C.. Senckenberg am Meer. Deutsches Zentrum für Marine Biodiversitätsforschung; Alemania
Fil: Hoppenrath, Mona. Senckenberg am Meer. Deutsches Zentrum für Marine Biodiversitätsforschung; Alemania
Materia
Coralline Crustose Algae
Filamentous Cyanobacteria
Macroalgal Germlings
Tube-Dwelling Diatoms
Turf Algae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37381
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopyFricke, Anna LenaKihara, Terue C.Hoppenrath, MonaCoralline Crustose AlgaeFilamentous CyanobacteriaMacroalgal GermlingsTube-Dwelling DiatomsTurf Algaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mesoalgae play a key role in shallow coastal ecosystems. Composed of small macroalgae, intermixed with filamentous cyanobacteria and colonial diatoms, these multi-specific, but minute (μm-cm) assemblages form complex three-dimensional structures, providing shelter for different unicellular (e.g. bacteria, diatoms, dinoflagellates) and multicellular (e.g. fishes, invertebrates) organisms. Characterized by a high colonization potential, these primary producers are observed to bloom and overgrow disturbed areas (e.g. damaged coral reefs, urchin barrens), and play a crucial role in terms of invasion and colonizing new habitats. Driven by anthropogenic environmental changes, mesoalgae are receiving considerable attention in current marine research. So far, most studies approach mesoalgae at the functional group level (e.g. turf algae, microphytobenthos), whereas only few studies tackle the importance of species-specific interactions, which play an important role in benthic ecology (e.g. coral-algal competition and disease spreading). To facilitate the study of not only the presence but also the composition and the structure of these habitat formers, we provide a new approach combining inexpensive fixation methodology with modern confocal laser scanning microscopy (CLSM), to study minute macroalgal structures (e.g. germlings, reproductive structures), and investigate their relation to microphytobenthic components (e.g. diatom colonies). Detailed procedures for mounting, staining and imaging phytobenthic communities are provided.Fil: Fricke, Anna Lena. Zentrum Fur Marine Tropenokologie Bremen; . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz Zentrum für Marine Tropenforschung; AlemaniaFil: Kihara, Terue C.. Senckenberg am Meer. Deutsches Zentrum für Marine Biodiversitätsforschung; AlemaniaFil: Hoppenrath, Mona. Senckenberg am Meer. Deutsches Zentrum für Marine Biodiversitätsforschung; AlemaniaDe Gruyter2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37381Fricke, Anna Lena; Kihara, Terue C.; Hoppenrath, Mona; Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy; De Gruyter; Botanica Marina; 60; 2; 4-2017; 181-1950006-8055CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1515/bot-2016-0057info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/botm.ahead-of-print/bot-2016-0057/bot-2016-0057.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:07Zoai:ri.conicet.gov.ar:11336/37381instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:07.914CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy
title Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy
spellingShingle Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy
Fricke, Anna Lena
Coralline Crustose Algae
Filamentous Cyanobacteria
Macroalgal Germlings
Tube-Dwelling Diatoms
Turf Algae
title_short Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy
title_full Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy
title_fullStr Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy
title_full_unstemmed Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy
title_sort Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy
dc.creator.none.fl_str_mv Fricke, Anna Lena
Kihara, Terue C.
Hoppenrath, Mona
author Fricke, Anna Lena
author_facet Fricke, Anna Lena
Kihara, Terue C.
Hoppenrath, Mona
author_role author
author2 Kihara, Terue C.
Hoppenrath, Mona
author2_role author
author
dc.subject.none.fl_str_mv Coralline Crustose Algae
Filamentous Cyanobacteria
Macroalgal Germlings
Tube-Dwelling Diatoms
Turf Algae
topic Coralline Crustose Algae
Filamentous Cyanobacteria
Macroalgal Germlings
Tube-Dwelling Diatoms
Turf Algae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mesoalgae play a key role in shallow coastal ecosystems. Composed of small macroalgae, intermixed with filamentous cyanobacteria and colonial diatoms, these multi-specific, but minute (μm-cm) assemblages form complex three-dimensional structures, providing shelter for different unicellular (e.g. bacteria, diatoms, dinoflagellates) and multicellular (e.g. fishes, invertebrates) organisms. Characterized by a high colonization potential, these primary producers are observed to bloom and overgrow disturbed areas (e.g. damaged coral reefs, urchin barrens), and play a crucial role in terms of invasion and colonizing new habitats. Driven by anthropogenic environmental changes, mesoalgae are receiving considerable attention in current marine research. So far, most studies approach mesoalgae at the functional group level (e.g. turf algae, microphytobenthos), whereas only few studies tackle the importance of species-specific interactions, which play an important role in benthic ecology (e.g. coral-algal competition and disease spreading). To facilitate the study of not only the presence but also the composition and the structure of these habitat formers, we provide a new approach combining inexpensive fixation methodology with modern confocal laser scanning microscopy (CLSM), to study minute macroalgal structures (e.g. germlings, reproductive structures), and investigate their relation to microphytobenthic components (e.g. diatom colonies). Detailed procedures for mounting, staining and imaging phytobenthic communities are provided.
Fil: Fricke, Anna Lena. Zentrum Fur Marine Tropenokologie Bremen; . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz Zentrum für Marine Tropenforschung; Alemania
Fil: Kihara, Terue C.. Senckenberg am Meer. Deutsches Zentrum für Marine Biodiversitätsforschung; Alemania
Fil: Hoppenrath, Mona. Senckenberg am Meer. Deutsches Zentrum für Marine Biodiversitätsforschung; Alemania
description Mesoalgae play a key role in shallow coastal ecosystems. Composed of small macroalgae, intermixed with filamentous cyanobacteria and colonial diatoms, these multi-specific, but minute (μm-cm) assemblages form complex three-dimensional structures, providing shelter for different unicellular (e.g. bacteria, diatoms, dinoflagellates) and multicellular (e.g. fishes, invertebrates) organisms. Characterized by a high colonization potential, these primary producers are observed to bloom and overgrow disturbed areas (e.g. damaged coral reefs, urchin barrens), and play a crucial role in terms of invasion and colonizing new habitats. Driven by anthropogenic environmental changes, mesoalgae are receiving considerable attention in current marine research. So far, most studies approach mesoalgae at the functional group level (e.g. turf algae, microphytobenthos), whereas only few studies tackle the importance of species-specific interactions, which play an important role in benthic ecology (e.g. coral-algal competition and disease spreading). To facilitate the study of not only the presence but also the composition and the structure of these habitat formers, we provide a new approach combining inexpensive fixation methodology with modern confocal laser scanning microscopy (CLSM), to study minute macroalgal structures (e.g. germlings, reproductive structures), and investigate their relation to microphytobenthic components (e.g. diatom colonies). Detailed procedures for mounting, staining and imaging phytobenthic communities are provided.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37381
Fricke, Anna Lena; Kihara, Terue C.; Hoppenrath, Mona; Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy; De Gruyter; Botanica Marina; 60; 2; 4-2017; 181-195
0006-8055
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37381
identifier_str_mv Fricke, Anna Lena; Kihara, Terue C.; Hoppenrath, Mona; Studying mesoalgal structures: A non-destructive approach based on confocal laser scanning microscopy; De Gruyter; Botanica Marina; 60; 2; 4-2017; 181-195
0006-8055
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1515/bot-2016-0057
info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/botm.ahead-of-print/bot-2016-0057/bot-2016-0057.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv De Gruyter
publisher.none.fl_str_mv De Gruyter
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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