Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation

Autores
Groff, Maria Carla; Noriega, Sandra Edith; Díaz Meglioli, María Eugenia; Rodríguez, Laura; Kuchen, Benjamín; Scaglia, Gustavo Juan Eduardo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Solid-state fermentation (SSF) is the bioprocess where microorganisms are cultivated in the absence of free water under controlled conditions. Lactic acid can be produced by Rhizopus oryzae SSF of grape stalks. During the microorganism’s growth, the temperature and water content of the solid bed fluctuate, leading to areas of either dry or excessive moisture in the solid substrate. Therefore, it is crucial to control the water supply to the matrix. In this work, we obtain lactic acid through SSF of grape stalks using Rhizopus oryzae NCIM 1299. The SSF was conducted at a fixed temperature of 35 ◦C, with five constant relative humidity (RH) levels: 50, 57, 65, 72, and 80%RH. Mathematical models, including the Logistic and First-Order Plus Dead-Time models for fungal biomass growth and the Luedeking and Piret with Delay Time model for lactic acid production, were adjusted to kinetic curves. Growth kinetic parameters (Xmax, μmax, Tp, T0, Yp/x, and td) were determined for all conditions. These kinetic parameters were then correlated with relative humidity using a seconddegree polynomial relationship. We observed a decrease in Xmax with an increasing %RH, while the value of Yp/x increased at a higher %RH. Finally, the optimal variable relative humidity profile was obtained by applying the dynamic optimization technique, resulting in a 16.63% increase in lactic acid production.
Fil: Groff, Maria Carla. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Noriega, Sandra Edith. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina
Fil: Díaz Meglioli, María Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina
Fil: Rodríguez, Laura. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Kuchen, Benjamín. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Scaglia, Gustavo Juan Eduardo. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina
Materia
DYNAMIC OPTIMIZATION
LACTIC ACID;
RHIZOPUS ORYZAE
SOLID-STATE FERMENTATION
VARIABLE HUMIDITY PROFILE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243288
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/243288
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State FermentationGroff, Maria CarlaNoriega, Sandra EdithDíaz Meglioli, María EugeniaRodríguez, LauraKuchen, BenjamínScaglia, Gustavo Juan EduardoDYNAMIC OPTIMIZATIONLACTIC ACID;RHIZOPUS ORYZAESOLID-STATE FERMENTATIONVARIABLE HUMIDITY PROFILEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Solid-state fermentation (SSF) is the bioprocess where microorganisms are cultivated in the absence of free water under controlled conditions. Lactic acid can be produced by Rhizopus oryzae SSF of grape stalks. During the microorganism’s growth, the temperature and water content of the solid bed fluctuate, leading to areas of either dry or excessive moisture in the solid substrate. Therefore, it is crucial to control the water supply to the matrix. In this work, we obtain lactic acid through SSF of grape stalks using Rhizopus oryzae NCIM 1299. The SSF was conducted at a fixed temperature of 35 ◦C, with five constant relative humidity (RH) levels: 50, 57, 65, 72, and 80%RH. Mathematical models, including the Logistic and First-Order Plus Dead-Time models for fungal biomass growth and the Luedeking and Piret with Delay Time model for lactic acid production, were adjusted to kinetic curves. Growth kinetic parameters (Xmax, μmax, Tp, T0, Yp/x, and td) were determined for all conditions. These kinetic parameters were then correlated with relative humidity using a seconddegree polynomial relationship. We observed a decrease in Xmax with an increasing %RH, while the value of Yp/x increased at a higher %RH. Finally, the optimal variable relative humidity profile was obtained by applying the dynamic optimization technique, resulting in a 16.63% increase in lactic acid production.Fil: Groff, Maria Carla. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Noriega, Sandra Edith. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; ArgentinaFil: Díaz Meglioli, María Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; ArgentinaFil: Rodríguez, Laura. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Kuchen, Benjamín. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Scaglia, Gustavo Juan Eduardo. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; ArgentinaMDPI2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243288Groff, Maria Carla; Noriega, Sandra Edith; Díaz Meglioli, María Eugenia; Rodríguez, Laura; Kuchen, Benjamín; et al.; Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation; MDPI; Fermentation; 10; 8; 8-2024; 1-142311-5637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/10/8/406info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation10080406info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:23:13Zoai:ri.conicet.gov.ar:11336/243288instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:23:14.328CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation
title Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation
spellingShingle Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation
Groff, Maria Carla
DYNAMIC OPTIMIZATION
LACTIC ACID;
RHIZOPUS ORYZAE
SOLID-STATE FERMENTATION
VARIABLE HUMIDITY PROFILE
title_short Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation
title_full Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation
title_fullStr Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation
title_full_unstemmed Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation
title_sort Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation
dc.creator.none.fl_str_mv Groff, Maria Carla
Noriega, Sandra Edith
Díaz Meglioli, María Eugenia
Rodríguez, Laura
Kuchen, Benjamín
Scaglia, Gustavo Juan Eduardo
author Groff, Maria Carla
author_facet Groff, Maria Carla
Noriega, Sandra Edith
Díaz Meglioli, María Eugenia
Rodríguez, Laura
Kuchen, Benjamín
Scaglia, Gustavo Juan Eduardo
author_role author
author2 Noriega, Sandra Edith
Díaz Meglioli, María Eugenia
Rodríguez, Laura
Kuchen, Benjamín
Scaglia, Gustavo Juan Eduardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DYNAMIC OPTIMIZATION
LACTIC ACID;
RHIZOPUS ORYZAE
SOLID-STATE FERMENTATION
VARIABLE HUMIDITY PROFILE
topic DYNAMIC OPTIMIZATION
LACTIC ACID;
RHIZOPUS ORYZAE
SOLID-STATE FERMENTATION
VARIABLE HUMIDITY PROFILE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Solid-state fermentation (SSF) is the bioprocess where microorganisms are cultivated in the absence of free water under controlled conditions. Lactic acid can be produced by Rhizopus oryzae SSF of grape stalks. During the microorganism’s growth, the temperature and water content of the solid bed fluctuate, leading to areas of either dry or excessive moisture in the solid substrate. Therefore, it is crucial to control the water supply to the matrix. In this work, we obtain lactic acid through SSF of grape stalks using Rhizopus oryzae NCIM 1299. The SSF was conducted at a fixed temperature of 35 ◦C, with five constant relative humidity (RH) levels: 50, 57, 65, 72, and 80%RH. Mathematical models, including the Logistic and First-Order Plus Dead-Time models for fungal biomass growth and the Luedeking and Piret with Delay Time model for lactic acid production, were adjusted to kinetic curves. Growth kinetic parameters (Xmax, μmax, Tp, T0, Yp/x, and td) were determined for all conditions. These kinetic parameters were then correlated with relative humidity using a seconddegree polynomial relationship. We observed a decrease in Xmax with an increasing %RH, while the value of Yp/x increased at a higher %RH. Finally, the optimal variable relative humidity profile was obtained by applying the dynamic optimization technique, resulting in a 16.63% increase in lactic acid production.
Fil: Groff, Maria Carla. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Noriega, Sandra Edith. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina
Fil: Díaz Meglioli, María Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina
Fil: Rodríguez, Laura. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Kuchen, Benjamín. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Scaglia, Gustavo Juan Eduardo. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Ingeniería Química; Argentina
description Solid-state fermentation (SSF) is the bioprocess where microorganisms are cultivated in the absence of free water under controlled conditions. Lactic acid can be produced by Rhizopus oryzae SSF of grape stalks. During the microorganism’s growth, the temperature and water content of the solid bed fluctuate, leading to areas of either dry or excessive moisture in the solid substrate. Therefore, it is crucial to control the water supply to the matrix. In this work, we obtain lactic acid through SSF of grape stalks using Rhizopus oryzae NCIM 1299. The SSF was conducted at a fixed temperature of 35 ◦C, with five constant relative humidity (RH) levels: 50, 57, 65, 72, and 80%RH. Mathematical models, including the Logistic and First-Order Plus Dead-Time models for fungal biomass growth and the Luedeking and Piret with Delay Time model for lactic acid production, were adjusted to kinetic curves. Growth kinetic parameters (Xmax, μmax, Tp, T0, Yp/x, and td) were determined for all conditions. These kinetic parameters were then correlated with relative humidity using a seconddegree polynomial relationship. We observed a decrease in Xmax with an increasing %RH, while the value of Yp/x increased at a higher %RH. Finally, the optimal variable relative humidity profile was obtained by applying the dynamic optimization technique, resulting in a 16.63% increase in lactic acid production.
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243288
Groff, Maria Carla; Noriega, Sandra Edith; Díaz Meglioli, María Eugenia; Rodríguez, Laura; Kuchen, Benjamín; et al.; Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation; MDPI; Fermentation; 10; 8; 8-2024; 1-14
2311-5637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243288
identifier_str_mv Groff, Maria Carla; Noriega, Sandra Edith; Díaz Meglioli, María Eugenia; Rodríguez, Laura; Kuchen, Benjamín; et al.; Determination of Variable Humidity Profile for Lactic Acid Maximization in Fungal Solid-State Fermentation; MDPI; Fermentation; 10; 8; 8-2024; 1-14
2311-5637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/10/8/406
info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation10080406
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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