Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
- Autores
- Bulgarella, Mariana; Peters, Jeffrey L.; Kopuchian, Cecilia; Valqui, Thomas; Wilson, Robert E.; Mc Cracken, Kevin G.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Hypoxia is a key factor determining survival, and haemoglobins are targets of selection in species native to high-altitude regions. We studied population genetic structure and evaluated evidence for local adaptation in the crested duck (Lophonetta specularioides). Differentiation, gene flow and time since divergence between highland and lowland populations were assessed for three haemoglobin genes (αA, αD, βA) and compared to seven reference loci (six autosomal introns and mtDNA). Four derived amino acid replacements were found in the globin genes that had elevated ΦST values between the Andean highlands and Patagonian lowlands. A single βA-globin polymorphism at a site known to influence O2 affinity was fixed for different alleles in the two populations, whereas three αA- and αD-globin polymorphisms exhibited high heterozygosity in the highlands but not in the lowlands. Coalescent analyses supported restricted gene flow for haemoglobin alleles and mitochondrial DNA but nonzero gene flow for the introns. Simulating genetic data under a drift–migration model of selective neutrality, the βA-globin fell outside the 95% confidence limit of simulated data, suggesting that directional selection is maintaining different variants in the contrasting elevational environments, thereby restricting migration of βA-globin alleles. The αA- and αD-globins, by contrast, did not differ from the simulated values, suggesting that variants in these genes are either selectively neutral, or that the effects of selection could not be differentiated from background levels of population structure and linkage disequilibrium. This study illustrates the combined effects of selection and population history on inferring levels of population divergence for a species distributed across an altitudinal gradient in which selection for hypoxia resistance has likely played an important role.
Fil: Bulgarella, Mariana. Massey University; Nueva Zelanda
Fil: Peters, Jeffrey L.. Wright State University; Estados Unidos
Fil: Kopuchian, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valqui, Thomas. Museum of Natural Science; Estados Unidos
Fil: Wilson, Robert E.. University Of Alaska; Estados Unidos
Fil: Mc Cracken, Kevin G.. University Of Alaska; Estados Unidos - Materia
-
Andes
Gene Flow
Haemoglobin
Hypoxia
Patagonia
Waterfowl
South America - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/26529
Ver los metadatos del registro completo
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Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)Bulgarella, MarianaPeters, Jeffrey L.Kopuchian, CeciliaValqui, ThomasWilson, Robert E.Mc Cracken, Kevin G.AndesGene FlowHaemoglobinHypoxiaPatagoniaWaterfowlSouth Americahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hypoxia is a key factor determining survival, and haemoglobins are targets of selection in species native to high-altitude regions. We studied population genetic structure and evaluated evidence for local adaptation in the crested duck (Lophonetta specularioides). Differentiation, gene flow and time since divergence between highland and lowland populations were assessed for three haemoglobin genes (αA, αD, βA) and compared to seven reference loci (six autosomal introns and mtDNA). Four derived amino acid replacements were found in the globin genes that had elevated ΦST values between the Andean highlands and Patagonian lowlands. A single βA-globin polymorphism at a site known to influence O2 affinity was fixed for different alleles in the two populations, whereas three αA- and αD-globin polymorphisms exhibited high heterozygosity in the highlands but not in the lowlands. Coalescent analyses supported restricted gene flow for haemoglobin alleles and mitochondrial DNA but nonzero gene flow for the introns. Simulating genetic data under a drift–migration model of selective neutrality, the βA-globin fell outside the 95% confidence limit of simulated data, suggesting that directional selection is maintaining different variants in the contrasting elevational environments, thereby restricting migration of βA-globin alleles. The αA- and αD-globins, by contrast, did not differ from the simulated values, suggesting that variants in these genes are either selectively neutral, or that the effects of selection could not be differentiated from background levels of population structure and linkage disequilibrium. This study illustrates the combined effects of selection and population history on inferring levels of population divergence for a species distributed across an altitudinal gradient in which selection for hypoxia resistance has likely played an important role.Fil: Bulgarella, Mariana. Massey University; Nueva ZelandaFil: Peters, Jeffrey L.. Wright State University; Estados UnidosFil: Kopuchian, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valqui, Thomas. Museum of Natural Science; Estados UnidosFil: Wilson, Robert E.. University Of Alaska; Estados UnidosFil: Mc Cracken, Kevin G.. University Of Alaska; Estados UnidosWiley Blackwell Publishing, Inc2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26529Bulgarella, Mariana; Peters, Jeffrey L.; Kopuchian, Cecilia; Valqui, Thomas; Wilson, Robert E.; et al.; Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides); Wiley Blackwell Publishing, Inc; Molecular Ecology; 21; 2; 12-2011; 350-3680962-10831365-294XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.2011.05400.xinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-294X.2011.05400.x/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:38Zoai:ri.conicet.gov.ar:11336/26529instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:39.187CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides) |
title |
Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides) |
spellingShingle |
Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides) Bulgarella, Mariana Andes Gene Flow Haemoglobin Hypoxia Patagonia Waterfowl South America |
title_short |
Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides) |
title_full |
Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides) |
title_fullStr |
Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides) |
title_full_unstemmed |
Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides) |
title_sort |
Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides) |
dc.creator.none.fl_str_mv |
Bulgarella, Mariana Peters, Jeffrey L. Kopuchian, Cecilia Valqui, Thomas Wilson, Robert E. Mc Cracken, Kevin G. |
author |
Bulgarella, Mariana |
author_facet |
Bulgarella, Mariana Peters, Jeffrey L. Kopuchian, Cecilia Valqui, Thomas Wilson, Robert E. Mc Cracken, Kevin G. |
author_role |
author |
author2 |
Peters, Jeffrey L. Kopuchian, Cecilia Valqui, Thomas Wilson, Robert E. Mc Cracken, Kevin G. |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Andes Gene Flow Haemoglobin Hypoxia Patagonia Waterfowl South America |
topic |
Andes Gene Flow Haemoglobin Hypoxia Patagonia Waterfowl South America |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Hypoxia is a key factor determining survival, and haemoglobins are targets of selection in species native to high-altitude regions. We studied population genetic structure and evaluated evidence for local adaptation in the crested duck (Lophonetta specularioides). Differentiation, gene flow and time since divergence between highland and lowland populations were assessed for three haemoglobin genes (αA, αD, βA) and compared to seven reference loci (six autosomal introns and mtDNA). Four derived amino acid replacements were found in the globin genes that had elevated ΦST values between the Andean highlands and Patagonian lowlands. A single βA-globin polymorphism at a site known to influence O2 affinity was fixed for different alleles in the two populations, whereas three αA- and αD-globin polymorphisms exhibited high heterozygosity in the highlands but not in the lowlands. Coalescent analyses supported restricted gene flow for haemoglobin alleles and mitochondrial DNA but nonzero gene flow for the introns. Simulating genetic data under a drift–migration model of selective neutrality, the βA-globin fell outside the 95% confidence limit of simulated data, suggesting that directional selection is maintaining different variants in the contrasting elevational environments, thereby restricting migration of βA-globin alleles. The αA- and αD-globins, by contrast, did not differ from the simulated values, suggesting that variants in these genes are either selectively neutral, or that the effects of selection could not be differentiated from background levels of population structure and linkage disequilibrium. This study illustrates the combined effects of selection and population history on inferring levels of population divergence for a species distributed across an altitudinal gradient in which selection for hypoxia resistance has likely played an important role. Fil: Bulgarella, Mariana. Massey University; Nueva Zelanda Fil: Peters, Jeffrey L.. Wright State University; Estados Unidos Fil: Kopuchian, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Valqui, Thomas. Museum of Natural Science; Estados Unidos Fil: Wilson, Robert E.. University Of Alaska; Estados Unidos Fil: Mc Cracken, Kevin G.. University Of Alaska; Estados Unidos |
description |
Hypoxia is a key factor determining survival, and haemoglobins are targets of selection in species native to high-altitude regions. We studied population genetic structure and evaluated evidence for local adaptation in the crested duck (Lophonetta specularioides). Differentiation, gene flow and time since divergence between highland and lowland populations were assessed for three haemoglobin genes (αA, αD, βA) and compared to seven reference loci (six autosomal introns and mtDNA). Four derived amino acid replacements were found in the globin genes that had elevated ΦST values between the Andean highlands and Patagonian lowlands. A single βA-globin polymorphism at a site known to influence O2 affinity was fixed for different alleles in the two populations, whereas three αA- and αD-globin polymorphisms exhibited high heterozygosity in the highlands but not in the lowlands. Coalescent analyses supported restricted gene flow for haemoglobin alleles and mitochondrial DNA but nonzero gene flow for the introns. Simulating genetic data under a drift–migration model of selective neutrality, the βA-globin fell outside the 95% confidence limit of simulated data, suggesting that directional selection is maintaining different variants in the contrasting elevational environments, thereby restricting migration of βA-globin alleles. The αA- and αD-globins, by contrast, did not differ from the simulated values, suggesting that variants in these genes are either selectively neutral, or that the effects of selection could not be differentiated from background levels of population structure and linkage disequilibrium. This study illustrates the combined effects of selection and population history on inferring levels of population divergence for a species distributed across an altitudinal gradient in which selection for hypoxia resistance has likely played an important role. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/26529 Bulgarella, Mariana; Peters, Jeffrey L.; Kopuchian, Cecilia; Valqui, Thomas; Wilson, Robert E.; et al.; Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides); Wiley Blackwell Publishing, Inc; Molecular Ecology; 21; 2; 12-2011; 350-368 0962-1083 1365-294X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/26529 |
identifier_str_mv |
Bulgarella, Mariana; Peters, Jeffrey L.; Kopuchian, Cecilia; Valqui, Thomas; Wilson, Robert E.; et al.; Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides); Wiley Blackwell Publishing, Inc; Molecular Ecology; 21; 2; 12-2011; 350-368 0962-1083 1365-294X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.2011.05400.x info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-294X.2011.05400.x/abstract |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614303375687680 |
score |
13.070432 |