Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)

Autores
Bulgarella, Mariana; Peters, Jeffrey L.; Kopuchian, Cecilia; Valqui, Thomas; Wilson, Robert E.; Mc Cracken, Kevin G.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hypoxia is a key factor determining survival, and haemoglobins are targets of selection in species native to high-altitude regions. We studied population genetic structure and evaluated evidence for local adaptation in the crested duck (Lophonetta specularioides). Differentiation, gene flow and time since divergence between highland and lowland populations were assessed for three haemoglobin genes (αA, αD, βA) and compared to seven reference loci (six autosomal introns and mtDNA). Four derived amino acid replacements were found in the globin genes that had elevated ΦST values between the Andean highlands and Patagonian lowlands. A single βA-globin polymorphism at a site known to influence O2 affinity was fixed for different alleles in the two populations, whereas three αA- and αD-globin polymorphisms exhibited high heterozygosity in the highlands but not in the lowlands. Coalescent analyses supported restricted gene flow for haemoglobin alleles and mitochondrial DNA but nonzero gene flow for the introns. Simulating genetic data under a drift–migration model of selective neutrality, the βA-globin fell outside the 95% confidence limit of simulated data, suggesting that directional selection is maintaining different variants in the contrasting elevational environments, thereby restricting migration of βA-globin alleles. The αA- and αD-globins, by contrast, did not differ from the simulated values, suggesting that variants in these genes are either selectively neutral, or that the effects of selection could not be differentiated from background levels of population structure and linkage disequilibrium. This study illustrates the combined effects of selection and population history on inferring levels of population divergence for a species distributed across an altitudinal gradient in which selection for hypoxia resistance has likely played an important role.
Fil: Bulgarella, Mariana. Massey University; Nueva Zelanda
Fil: Peters, Jeffrey L.. Wright State University; Estados Unidos
Fil: Kopuchian, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valqui, Thomas. Museum of Natural Science; Estados Unidos
Fil: Wilson, Robert E.. University Of Alaska; Estados Unidos
Fil: Mc Cracken, Kevin G.. University Of Alaska; Estados Unidos
Materia
Andes
Gene Flow
Haemoglobin
Hypoxia
Patagonia
Waterfowl
South America
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26529

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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)Bulgarella, MarianaPeters, Jeffrey L.Kopuchian, CeciliaValqui, ThomasWilson, Robert E.Mc Cracken, Kevin G.AndesGene FlowHaemoglobinHypoxiaPatagoniaWaterfowlSouth Americahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hypoxia is a key factor determining survival, and haemoglobins are targets of selection in species native to high-altitude regions. We studied population genetic structure and evaluated evidence for local adaptation in the crested duck (Lophonetta specularioides). Differentiation, gene flow and time since divergence between highland and lowland populations were assessed for three haemoglobin genes (αA, αD, βA) and compared to seven reference loci (six autosomal introns and mtDNA). Four derived amino acid replacements were found in the globin genes that had elevated ΦST values between the Andean highlands and Patagonian lowlands. A single βA-globin polymorphism at a site known to influence O2 affinity was fixed for different alleles in the two populations, whereas three αA- and αD-globin polymorphisms exhibited high heterozygosity in the highlands but not in the lowlands. Coalescent analyses supported restricted gene flow for haemoglobin alleles and mitochondrial DNA but nonzero gene flow for the introns. Simulating genetic data under a drift–migration model of selective neutrality, the βA-globin fell outside the 95% confidence limit of simulated data, suggesting that directional selection is maintaining different variants in the contrasting elevational environments, thereby restricting migration of βA-globin alleles. The αA- and αD-globins, by contrast, did not differ from the simulated values, suggesting that variants in these genes are either selectively neutral, or that the effects of selection could not be differentiated from background levels of population structure and linkage disequilibrium. This study illustrates the combined effects of selection and population history on inferring levels of population divergence for a species distributed across an altitudinal gradient in which selection for hypoxia resistance has likely played an important role.Fil: Bulgarella, Mariana. Massey University; Nueva ZelandaFil: Peters, Jeffrey L.. Wright State University; Estados UnidosFil: Kopuchian, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valqui, Thomas. Museum of Natural Science; Estados UnidosFil: Wilson, Robert E.. University Of Alaska; Estados UnidosFil: Mc Cracken, Kevin G.. University Of Alaska; Estados UnidosWiley Blackwell Publishing, Inc2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26529Bulgarella, Mariana; Peters, Jeffrey L.; Kopuchian, Cecilia; Valqui, Thomas; Wilson, Robert E.; et al.; Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides); Wiley Blackwell Publishing, Inc; Molecular Ecology; 21; 2; 12-2011; 350-3680962-10831365-294XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.2011.05400.xinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-294X.2011.05400.x/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:38Zoai:ri.conicet.gov.ar:11336/26529instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:39.187CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
title Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
spellingShingle Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
Bulgarella, Mariana
Andes
Gene Flow
Haemoglobin
Hypoxia
Patagonia
Waterfowl
South America
title_short Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
title_full Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
title_fullStr Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
title_full_unstemmed Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
title_sort Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides)
dc.creator.none.fl_str_mv Bulgarella, Mariana
Peters, Jeffrey L.
Kopuchian, Cecilia
Valqui, Thomas
Wilson, Robert E.
Mc Cracken, Kevin G.
author Bulgarella, Mariana
author_facet Bulgarella, Mariana
Peters, Jeffrey L.
Kopuchian, Cecilia
Valqui, Thomas
Wilson, Robert E.
Mc Cracken, Kevin G.
author_role author
author2 Peters, Jeffrey L.
Kopuchian, Cecilia
Valqui, Thomas
Wilson, Robert E.
Mc Cracken, Kevin G.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Andes
Gene Flow
Haemoglobin
Hypoxia
Patagonia
Waterfowl
South America
topic Andes
Gene Flow
Haemoglobin
Hypoxia
Patagonia
Waterfowl
South America
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hypoxia is a key factor determining survival, and haemoglobins are targets of selection in species native to high-altitude regions. We studied population genetic structure and evaluated evidence for local adaptation in the crested duck (Lophonetta specularioides). Differentiation, gene flow and time since divergence between highland and lowland populations were assessed for three haemoglobin genes (αA, αD, βA) and compared to seven reference loci (six autosomal introns and mtDNA). Four derived amino acid replacements were found in the globin genes that had elevated ΦST values between the Andean highlands and Patagonian lowlands. A single βA-globin polymorphism at a site known to influence O2 affinity was fixed for different alleles in the two populations, whereas three αA- and αD-globin polymorphisms exhibited high heterozygosity in the highlands but not in the lowlands. Coalescent analyses supported restricted gene flow for haemoglobin alleles and mitochondrial DNA but nonzero gene flow for the introns. Simulating genetic data under a drift–migration model of selective neutrality, the βA-globin fell outside the 95% confidence limit of simulated data, suggesting that directional selection is maintaining different variants in the contrasting elevational environments, thereby restricting migration of βA-globin alleles. The αA- and αD-globins, by contrast, did not differ from the simulated values, suggesting that variants in these genes are either selectively neutral, or that the effects of selection could not be differentiated from background levels of population structure and linkage disequilibrium. This study illustrates the combined effects of selection and population history on inferring levels of population divergence for a species distributed across an altitudinal gradient in which selection for hypoxia resistance has likely played an important role.
Fil: Bulgarella, Mariana. Massey University; Nueva Zelanda
Fil: Peters, Jeffrey L.. Wright State University; Estados Unidos
Fil: Kopuchian, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valqui, Thomas. Museum of Natural Science; Estados Unidos
Fil: Wilson, Robert E.. University Of Alaska; Estados Unidos
Fil: Mc Cracken, Kevin G.. University Of Alaska; Estados Unidos
description Hypoxia is a key factor determining survival, and haemoglobins are targets of selection in species native to high-altitude regions. We studied population genetic structure and evaluated evidence for local adaptation in the crested duck (Lophonetta specularioides). Differentiation, gene flow and time since divergence between highland and lowland populations were assessed for three haemoglobin genes (αA, αD, βA) and compared to seven reference loci (six autosomal introns and mtDNA). Four derived amino acid replacements were found in the globin genes that had elevated ΦST values between the Andean highlands and Patagonian lowlands. A single βA-globin polymorphism at a site known to influence O2 affinity was fixed for different alleles in the two populations, whereas three αA- and αD-globin polymorphisms exhibited high heterozygosity in the highlands but not in the lowlands. Coalescent analyses supported restricted gene flow for haemoglobin alleles and mitochondrial DNA but nonzero gene flow for the introns. Simulating genetic data under a drift–migration model of selective neutrality, the βA-globin fell outside the 95% confidence limit of simulated data, suggesting that directional selection is maintaining different variants in the contrasting elevational environments, thereby restricting migration of βA-globin alleles. The αA- and αD-globins, by contrast, did not differ from the simulated values, suggesting that variants in these genes are either selectively neutral, or that the effects of selection could not be differentiated from background levels of population structure and linkage disequilibrium. This study illustrates the combined effects of selection and population history on inferring levels of population divergence for a species distributed across an altitudinal gradient in which selection for hypoxia resistance has likely played an important role.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26529
Bulgarella, Mariana; Peters, Jeffrey L.; Kopuchian, Cecilia; Valqui, Thomas; Wilson, Robert E.; et al.; Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides); Wiley Blackwell Publishing, Inc; Molecular Ecology; 21; 2; 12-2011; 350-368
0962-1083
1365-294X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26529
identifier_str_mv Bulgarella, Mariana; Peters, Jeffrey L.; Kopuchian, Cecilia; Valqui, Thomas; Wilson, Robert E.; et al.; Multilocus coalescent analysis of hemoglobin differentiation between low- and high-altitude populations of crested ducks (lophonetta specularioides); Wiley Blackwell Publishing, Inc; Molecular Ecology; 21; 2; 12-2011; 350-368
0962-1083
1365-294X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.2011.05400.x
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-294X.2011.05400.x/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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