Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4

Autores
Ribichich, Karina Fabiana; Chiozza, Mariana; Ávalos-Britez, Selva; Cabello, Julieta Virginia; Arce, Agustín Lucas; Watson, Geronimo; Arias, Claudia Vanina; Portapila, Margarita Isabel; Trucco, Federico; Otegui, Maria Elena; Chan, Raquel Lia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic-stress tolerance is available in the market. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat plants. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield (SY). Such line was evaluated in the greenhouse and in twenty-seven field trials developed in different environments of Argentina. In greenhouse experiments, transgenic plants showed increased SY under stress conditions together with wider epycotyl diameter, enlarged xylem area and enhanced water use efficiency than controls. They also exhibited enhanced SY in warm-dry field conditions. This response was accompanied by the increased in seed number that was not compensated by the decreased in individual seed weight. The transcriptome analysis of plants from a field trial with maximum SY difference between genotypes indicated an induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have reduced SY penalization when cropped in warm-dry conditions, which constitute the best target environments for this technology.
Fil: Ribichich, Karina Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Chiozza, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Ávalos-Britez, Selva. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cabello, Julieta Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Watson, Geronimo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Arias, Claudia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina
Fil: Portapila, Margarita Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina
Fil: Trucco, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Otegui, Maria Elena. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
TRANSGENIC SOYBEAN
HAHB4
DROUGHT TOLERANCE
SOYBEAN FIELD TRIALS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111219
Acceder
id CONICETDig_5b04bc7184323e6443013bc38c2371c9
oai_identifier_str oai:ri.conicet.gov.ar:11336/111219
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4Ribichich, Karina FabianaChiozza, MarianaÁvalos-Britez, SelvaCabello, Julieta VirginiaArce, Agustín LucasWatson, GeronimoArias, Claudia VaninaPortapila, Margarita IsabelTrucco, FedericoOtegui, Maria ElenaChan, Raquel LiaTRANSGENIC SOYBEANHAHB4DROUGHT TOLERANCESOYBEAN FIELD TRIALShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic-stress tolerance is available in the market. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat plants. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield (SY). Such line was evaluated in the greenhouse and in twenty-seven field trials developed in different environments of Argentina. In greenhouse experiments, transgenic plants showed increased SY under stress conditions together with wider epycotyl diameter, enlarged xylem area and enhanced water use efficiency than controls. They also exhibited enhanced SY in warm-dry field conditions. This response was accompanied by the increased in seed number that was not compensated by the decreased in individual seed weight. The transcriptome analysis of plants from a field trial with maximum SY difference between genotypes indicated an induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have reduced SY penalization when cropped in warm-dry conditions, which constitute the best target environments for this technology.Fil: Ribichich, Karina Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Chiozza, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Ávalos-Britez, Selva. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Cabello, Julieta Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Watson, Geronimo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Arias, Claudia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; ArgentinaFil: Portapila, Margarita Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; ArgentinaFil: Trucco, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Otegui, Maria Elena. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaOxford University Press2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111219Ribichich, Karina Fabiana; Chiozza, Mariana; Ávalos-Britez, Selva; Cabello, Julieta Virginia; Arce, Agustín Lucas; et al.; Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4; Oxford University Press; Journal of Experimental Botany; 71; 10; 3-2020; 3142–31560022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/eraa064/5788192info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/eraa064info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:15Zoai:ri.conicet.gov.ar:11336/111219instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:15.409CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4
title Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4
spellingShingle Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4
Ribichich, Karina Fabiana
TRANSGENIC SOYBEAN
HAHB4
DROUGHT TOLERANCE
SOYBEAN FIELD TRIALS
title_short Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4
title_full Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4
title_fullStr Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4
title_full_unstemmed Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4
title_sort Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4
dc.creator.none.fl_str_mv Ribichich, Karina Fabiana
Chiozza, Mariana
Ávalos-Britez, Selva
Cabello, Julieta Virginia
Arce, Agustín Lucas
Watson, Geronimo
Arias, Claudia Vanina
Portapila, Margarita Isabel
Trucco, Federico
Otegui, Maria Elena
Chan, Raquel Lia
author Ribichich, Karina Fabiana
author_facet Ribichich, Karina Fabiana
Chiozza, Mariana
Ávalos-Britez, Selva
Cabello, Julieta Virginia
Arce, Agustín Lucas
Watson, Geronimo
Arias, Claudia Vanina
Portapila, Margarita Isabel
Trucco, Federico
Otegui, Maria Elena
Chan, Raquel Lia
author_role author
author2 Chiozza, Mariana
Ávalos-Britez, Selva
Cabello, Julieta Virginia
Arce, Agustín Lucas
Watson, Geronimo
Arias, Claudia Vanina
Portapila, Margarita Isabel
Trucco, Federico
Otegui, Maria Elena
Chan, Raquel Lia
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRANSGENIC SOYBEAN
HAHB4
DROUGHT TOLERANCE
SOYBEAN FIELD TRIALS
topic TRANSGENIC SOYBEAN
HAHB4
DROUGHT TOLERANCE
SOYBEAN FIELD TRIALS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic-stress tolerance is available in the market. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat plants. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield (SY). Such line was evaluated in the greenhouse and in twenty-seven field trials developed in different environments of Argentina. In greenhouse experiments, transgenic plants showed increased SY under stress conditions together with wider epycotyl diameter, enlarged xylem area and enhanced water use efficiency than controls. They also exhibited enhanced SY in warm-dry field conditions. This response was accompanied by the increased in seed number that was not compensated by the decreased in individual seed weight. The transcriptome analysis of plants from a field trial with maximum SY difference between genotypes indicated an induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have reduced SY penalization when cropped in warm-dry conditions, which constitute the best target environments for this technology.
Fil: Ribichich, Karina Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Chiozza, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Ávalos-Britez, Selva. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cabello, Julieta Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Watson, Geronimo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Arias, Claudia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina
Fil: Portapila, Margarita Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas. Universidad Nacional de Rosario. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina
Fil: Trucco, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Otegui, Maria Elena. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic-stress tolerance is available in the market. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat plants. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield (SY). Such line was evaluated in the greenhouse and in twenty-seven field trials developed in different environments of Argentina. In greenhouse experiments, transgenic plants showed increased SY under stress conditions together with wider epycotyl diameter, enlarged xylem area and enhanced water use efficiency than controls. They also exhibited enhanced SY in warm-dry field conditions. This response was accompanied by the increased in seed number that was not compensated by the decreased in individual seed weight. The transcriptome analysis of plants from a field trial with maximum SY difference between genotypes indicated an induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have reduced SY penalization when cropped in warm-dry conditions, which constitute the best target environments for this technology.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111219
Ribichich, Karina Fabiana; Chiozza, Mariana; Ávalos-Britez, Selva; Cabello, Julieta Virginia; Arce, Agustín Lucas; et al.; Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4; Oxford University Press; Journal of Experimental Botany; 71; 10; 3-2020; 3142–3156
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111219
identifier_str_mv Ribichich, Karina Fabiana; Chiozza, Mariana; Ávalos-Britez, Selva; Cabello, Julieta Virginia; Arce, Agustín Lucas; et al.; Successful field performance in dry-warm environments of soybean expressing the sunflower transcription factor HaHB4; Oxford University Press; Journal of Experimental Botany; 71; 10; 3-2020; 3142–3156
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/eraa064/5788192
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/eraa064
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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