Changes in prolactin receptor location in prostate tumors

Autores
López Fontana, Gastón; Rey Echalecu, Lara Sofía; Santiano, Flavia Eliana; López Fontana, José Rodrigo; López Laur, José Daniel; Zyla, Leila Ester; Valdemoros, Paula; Guerrero Gimenez, Martin Eduardo; Fernandez Muñoz, Juan Manuel; Gomez, Silvina Esther; Bruna, Flavia Alejandra; Guglielmi, Juan Manuel; Caron, Ruben Walter; López Fontana, Constanza Matilde
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Prolactin (PRL) binds its receptor (PRLR) and stimulates cell proliferation, differentiation and survival in prostate cancer (PCa) cell lines via STAT5a, MAPK and AKT. Objective: To evaluate the expression of PRL and PRLR in normal and tumor prostate tissues with different Gleason patterns. Methods: Samples of normal, benign prostatic hyperplasia and PCa with different Gleason patterns were selected from radical prostatectomy. The intensity, location and percentage of stained cells for PRL and PRLR were evaluated by Immunohistochemistry. Co-localization was observed by confocal microscopy. Results: PRL was expressed diffusely and with a mild intensity in the cytoplasm of normal and tumor prostate luminal cells. Its expression only augmented in the Gleason 3 pattern (p 0.0001). The immunostaining intensity and the percentage of positive cells for PRLR did not vary between normal and tumor tissues. However, the location of the PRLR was modified by the tumorigenic process. In non-tumor tissues, PRLR expression was mostly in plasma membrane in the apical zone of epithelial cells. In tumor tissues, it was expressed in intracellular vesicles. The co-localization of PRL and PRLR was demonstrated in normal and tumor tissues suggesting that PRL could be acting in an autocrine and paracrine manner. Conclusion: PRL and its receptor were present in the cytoplasm of the epithelial cells of the normal and tumor prostate gland. In tumor tissues, the change in the location and appearance of cryptic PRLRs that store PRL may keep active the different signaling pathways related to cell proliferation and survival.
Fil: López Fontana, Gastón. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Clinica Andina de Urología; Argentina
Fil: Rey Echalecu, Lara Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Santiano, Flavia Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: López Fontana, José Rodrigo. Clinica Andina de Urología; Argentina
Fil: López Laur, José Daniel. Clinica Andina de Urología; Argentina
Fil: Zyla, Leila Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Valdemoros, Paula. Laboratorio de Anatomía Patológica Vega Valdemoros; Argentina
Fil: Guerrero Gimenez, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Fernandez Muñoz, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Gomez, Silvina Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Bruna, Flavia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Guglielmi, Juan Manuel. Clinica Andina de Urología; Argentina
Fil: Caron, Ruben Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: López Fontana, Constanza Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
PROLACTIN
PROSTATE CANCER
PROLACTIN RECEPTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173012
Acceder
id CONICETDig_5af279709be9e9baa5e04b0f30adcfd9
oai_identifier_str oai:ri.conicet.gov.ar:11336/173012
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in prolactin receptor location in prostate tumorsLópez Fontana, GastónRey Echalecu, Lara SofíaSantiano, Flavia ElianaLópez Fontana, José RodrigoLópez Laur, José DanielZyla, Leila EsterValdemoros, PaulaGuerrero Gimenez, Martin EduardoFernandez Muñoz, Juan ManuelGomez, Silvina EstherBruna, Flavia AlejandraGuglielmi, Juan ManuelCaron, Ruben WalterLópez Fontana, Constanza MatildePROLACTINPROSTATE CANCERPROLACTIN RECEPTORhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Introduction: Prolactin (PRL) binds its receptor (PRLR) and stimulates cell proliferation, differentiation and survival in prostate cancer (PCa) cell lines via STAT5a, MAPK and AKT. Objective: To evaluate the expression of PRL and PRLR in normal and tumor prostate tissues with different Gleason patterns. Methods: Samples of normal, benign prostatic hyperplasia and PCa with different Gleason patterns were selected from radical prostatectomy. The intensity, location and percentage of stained cells for PRL and PRLR were evaluated by Immunohistochemistry. Co-localization was observed by confocal microscopy. Results: PRL was expressed diffusely and with a mild intensity in the cytoplasm of normal and tumor prostate luminal cells. Its expression only augmented in the Gleason 3 pattern (p 0.0001). The immunostaining intensity and the percentage of positive cells for PRLR did not vary between normal and tumor tissues. However, the location of the PRLR was modified by the tumorigenic process. In non-tumor tissues, PRLR expression was mostly in plasma membrane in the apical zone of epithelial cells. In tumor tissues, it was expressed in intracellular vesicles. The co-localization of PRL and PRLR was demonstrated in normal and tumor tissues suggesting that PRL could be acting in an autocrine and paracrine manner. Conclusion: PRL and its receptor were present in the cytoplasm of the epithelial cells of the normal and tumor prostate gland. In tumor tissues, the change in the location and appearance of cryptic PRLRs that store PRL may keep active the different signaling pathways related to cell proliferation and survival.Fil: López Fontana, Gastón. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Clinica Andina de Urología; ArgentinaFil: Rey Echalecu, Lara Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Santiano, Flavia Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: López Fontana, José Rodrigo. Clinica Andina de Urología; ArgentinaFil: López Laur, José Daniel. Clinica Andina de Urología; ArgentinaFil: Zyla, Leila Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Valdemoros, Paula. Laboratorio de Anatomía Patológica Vega Valdemoros; ArgentinaFil: Guerrero Gimenez, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Fernandez Muñoz, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Gomez, Silvina Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Bruna, Flavia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Guglielmi, Juan Manuel. Clinica Andina de Urología; ArgentinaFil: Caron, Ruben Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: López Fontana, Constanza Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaIniestares2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/173012López Fontana, Gastón; Rey Echalecu, Lara Sofía; Santiano, Flavia Eliana; López Fontana, José Rodrigo; López Laur, José Daniel; et al.; Changes in prolactin receptor location in prostate tumors; Iniestares; Archivos Españoles de Urología; 74; 4; 4-2021; 419-4260004-06141576-8260CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.aeurologia.com/EN/Y2021/V74/I4/419info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:16:25Zoai:ri.conicet.gov.ar:11336/173012instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:16:25.63CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in prolactin receptor location in prostate tumors
title Changes in prolactin receptor location in prostate tumors
spellingShingle Changes in prolactin receptor location in prostate tumors
López Fontana, Gastón
PROLACTIN
PROSTATE CANCER
PROLACTIN RECEPTOR
title_short Changes in prolactin receptor location in prostate tumors
title_full Changes in prolactin receptor location in prostate tumors
title_fullStr Changes in prolactin receptor location in prostate tumors
title_full_unstemmed Changes in prolactin receptor location in prostate tumors
title_sort Changes in prolactin receptor location in prostate tumors
dc.creator.none.fl_str_mv López Fontana, Gastón
Rey Echalecu, Lara Sofía
Santiano, Flavia Eliana
López Fontana, José Rodrigo
López Laur, José Daniel
Zyla, Leila Ester
Valdemoros, Paula
Guerrero Gimenez, Martin Eduardo
Fernandez Muñoz, Juan Manuel
Gomez, Silvina Esther
Bruna, Flavia Alejandra
Guglielmi, Juan Manuel
Caron, Ruben Walter
López Fontana, Constanza Matilde
author López Fontana, Gastón
author_facet López Fontana, Gastón
Rey Echalecu, Lara Sofía
Santiano, Flavia Eliana
López Fontana, José Rodrigo
López Laur, José Daniel
Zyla, Leila Ester
Valdemoros, Paula
Guerrero Gimenez, Martin Eduardo
Fernandez Muñoz, Juan Manuel
Gomez, Silvina Esther
Bruna, Flavia Alejandra
Guglielmi, Juan Manuel
Caron, Ruben Walter
López Fontana, Constanza Matilde
author_role author
author2 Rey Echalecu, Lara Sofía
Santiano, Flavia Eliana
López Fontana, José Rodrigo
López Laur, José Daniel
Zyla, Leila Ester
Valdemoros, Paula
Guerrero Gimenez, Martin Eduardo
Fernandez Muñoz, Juan Manuel
Gomez, Silvina Esther
Bruna, Flavia Alejandra
Guglielmi, Juan Manuel
Caron, Ruben Walter
López Fontana, Constanza Matilde
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PROLACTIN
PROSTATE CANCER
PROLACTIN RECEPTOR
topic PROLACTIN
PROSTATE CANCER
PROLACTIN RECEPTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: Prolactin (PRL) binds its receptor (PRLR) and stimulates cell proliferation, differentiation and survival in prostate cancer (PCa) cell lines via STAT5a, MAPK and AKT. Objective: To evaluate the expression of PRL and PRLR in normal and tumor prostate tissues with different Gleason patterns. Methods: Samples of normal, benign prostatic hyperplasia and PCa with different Gleason patterns were selected from radical prostatectomy. The intensity, location and percentage of stained cells for PRL and PRLR were evaluated by Immunohistochemistry. Co-localization was observed by confocal microscopy. Results: PRL was expressed diffusely and with a mild intensity in the cytoplasm of normal and tumor prostate luminal cells. Its expression only augmented in the Gleason 3 pattern (p 0.0001). The immunostaining intensity and the percentage of positive cells for PRLR did not vary between normal and tumor tissues. However, the location of the PRLR was modified by the tumorigenic process. In non-tumor tissues, PRLR expression was mostly in plasma membrane in the apical zone of epithelial cells. In tumor tissues, it was expressed in intracellular vesicles. The co-localization of PRL and PRLR was demonstrated in normal and tumor tissues suggesting that PRL could be acting in an autocrine and paracrine manner. Conclusion: PRL and its receptor were present in the cytoplasm of the epithelial cells of the normal and tumor prostate gland. In tumor tissues, the change in the location and appearance of cryptic PRLRs that store PRL may keep active the different signaling pathways related to cell proliferation and survival.
Fil: López Fontana, Gastón. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Clinica Andina de Urología; Argentina
Fil: Rey Echalecu, Lara Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Santiano, Flavia Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: López Fontana, José Rodrigo. Clinica Andina de Urología; Argentina
Fil: López Laur, José Daniel. Clinica Andina de Urología; Argentina
Fil: Zyla, Leila Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Valdemoros, Paula. Laboratorio de Anatomía Patológica Vega Valdemoros; Argentina
Fil: Guerrero Gimenez, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Fernandez Muñoz, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Gomez, Silvina Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Bruna, Flavia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Guglielmi, Juan Manuel. Clinica Andina de Urología; Argentina
Fil: Caron, Ruben Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: López Fontana, Constanza Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description Introduction: Prolactin (PRL) binds its receptor (PRLR) and stimulates cell proliferation, differentiation and survival in prostate cancer (PCa) cell lines via STAT5a, MAPK and AKT. Objective: To evaluate the expression of PRL and PRLR in normal and tumor prostate tissues with different Gleason patterns. Methods: Samples of normal, benign prostatic hyperplasia and PCa with different Gleason patterns were selected from radical prostatectomy. The intensity, location and percentage of stained cells for PRL and PRLR were evaluated by Immunohistochemistry. Co-localization was observed by confocal microscopy. Results: PRL was expressed diffusely and with a mild intensity in the cytoplasm of normal and tumor prostate luminal cells. Its expression only augmented in the Gleason 3 pattern (p 0.0001). The immunostaining intensity and the percentage of positive cells for PRLR did not vary between normal and tumor tissues. However, the location of the PRLR was modified by the tumorigenic process. In non-tumor tissues, PRLR expression was mostly in plasma membrane in the apical zone of epithelial cells. In tumor tissues, it was expressed in intracellular vesicles. The co-localization of PRL and PRLR was demonstrated in normal and tumor tissues suggesting that PRL could be acting in an autocrine and paracrine manner. Conclusion: PRL and its receptor were present in the cytoplasm of the epithelial cells of the normal and tumor prostate gland. In tumor tissues, the change in the location and appearance of cryptic PRLRs that store PRL may keep active the different signaling pathways related to cell proliferation and survival.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173012
López Fontana, Gastón; Rey Echalecu, Lara Sofía; Santiano, Flavia Eliana; López Fontana, José Rodrigo; López Laur, José Daniel; et al.; Changes in prolactin receptor location in prostate tumors; Iniestares; Archivos Españoles de Urología; 74; 4; 4-2021; 419-426
0004-0614
1576-8260
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173012
identifier_str_mv López Fontana, Gastón; Rey Echalecu, Lara Sofía; Santiano, Flavia Eliana; López Fontana, José Rodrigo; López Laur, José Daniel; et al.; Changes in prolactin receptor location in prostate tumors; Iniestares; Archivos Españoles de Urología; 74; 4; 4-2021; 419-426
0004-0614
1576-8260
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.aeurologia.com/EN/Y2021/V74/I4/419
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Iniestares
publisher.none.fl_str_mv Iniestares
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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