Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation

Autores
Bédard, Andréanne; Tremblay, Pierrot; Chernomoretz, Ariel; Vallières, Luc
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microglia, monocytes, and peripheral macrophages share a common origin and many characteristics, but what distinguishes them from each other at the level of gene expression remains largely unknown. In this study, we compared the transcriptional profiles of freshly purified microglia, monocytes, and spleen macrophages using Affymetrix Mouse Genome arrays to identify genes predominantly expressed by microglia. Among tens of thousands of genes assayed, 127 potential candidates were found, including nine newly discovered genes encoding plasma membrane and extracellular proteins. In the brain, the latter were selectively expressed by microglia, as revealed by in situ hybridization. Three of them were confirmed to be exclusively (MSR2) or predominantly (GPR12, GPR34) expressed in the brain compared to the other tissues examined. Furthermore, all of these genes were upregulated in activated microglia after treatment with the demyelinating toxin cuprizone, suggesting that they play roles in neuroinflammation. In conclusion, this study reports the identification of new selective markers for microglia, which should prove useful not only to identify and isolate these cells, but also to better understand their distinctive properties. © 2007 Wiley-Liss, Inc.
Fil: Bédard, Andréanne. Laval University; Canadá
Fil: Tremblay, Pierrot. Laval University; Canadá
Fil: Chernomoretz, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laval University; Canadá
Fil: Vallières, Luc. Laval University; Canadá
Materia
Cuprizone
Demyelination
Dna Microarray
G Protein-Coupled Receptor
Gene Profiling
Microglia
Monocytes
Spleen Macrophages
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/70606
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/70606
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammationBédard, AndréanneTremblay, PierrotChernomoretz, ArielVallières, LucCuprizoneDemyelinationDna MicroarrayG Protein-Coupled ReceptorGene ProfilingMicrogliaMonocytesSpleen Macrophageshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microglia, monocytes, and peripheral macrophages share a common origin and many characteristics, but what distinguishes them from each other at the level of gene expression remains largely unknown. In this study, we compared the transcriptional profiles of freshly purified microglia, monocytes, and spleen macrophages using Affymetrix Mouse Genome arrays to identify genes predominantly expressed by microglia. Among tens of thousands of genes assayed, 127 potential candidates were found, including nine newly discovered genes encoding plasma membrane and extracellular proteins. In the brain, the latter were selectively expressed by microglia, as revealed by in situ hybridization. Three of them were confirmed to be exclusively (MSR2) or predominantly (GPR12, GPR34) expressed in the brain compared to the other tissues examined. Furthermore, all of these genes were upregulated in activated microglia after treatment with the demyelinating toxin cuprizone, suggesting that they play roles in neuroinflammation. In conclusion, this study reports the identification of new selective markers for microglia, which should prove useful not only to identify and isolate these cells, but also to better understand their distinctive properties. © 2007 Wiley-Liss, Inc.Fil: Bédard, Andréanne. Laval University; CanadáFil: Tremblay, Pierrot. Laval University; CanadáFil: Chernomoretz, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laval University; CanadáFil: Vallières, Luc. Laval University; CanadáWiley-liss, Div John Wiley & Sons Inc2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/70606Bédard, Andréanne; Tremblay, Pierrot; Chernomoretz, Ariel; Vallières, Luc; Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation; Wiley-liss, Div John Wiley & Sons Inc; Glia; 55; 8; 6-2007; 777-7890894-1491CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/glia.20477info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.20477info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:03Zoai:ri.conicet.gov.ar:11336/70606instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:03.963CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation
title Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation
spellingShingle Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation
Bédard, Andréanne
Cuprizone
Demyelination
Dna Microarray
G Protein-Coupled Receptor
Gene Profiling
Microglia
Monocytes
Spleen Macrophages
title_short Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation
title_full Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation
title_fullStr Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation
title_full_unstemmed Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation
title_sort Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation
dc.creator.none.fl_str_mv Bédard, Andréanne
Tremblay, Pierrot
Chernomoretz, Ariel
Vallières, Luc
author Bédard, Andréanne
author_facet Bédard, Andréanne
Tremblay, Pierrot
Chernomoretz, Ariel
Vallières, Luc
author_role author
author2 Tremblay, Pierrot
Chernomoretz, Ariel
Vallières, Luc
author2_role author
author
author
dc.subject.none.fl_str_mv Cuprizone
Demyelination
Dna Microarray
G Protein-Coupled Receptor
Gene Profiling
Microglia
Monocytes
Spleen Macrophages
topic Cuprizone
Demyelination
Dna Microarray
G Protein-Coupled Receptor
Gene Profiling
Microglia
Monocytes
Spleen Macrophages
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microglia, monocytes, and peripheral macrophages share a common origin and many characteristics, but what distinguishes them from each other at the level of gene expression remains largely unknown. In this study, we compared the transcriptional profiles of freshly purified microglia, monocytes, and spleen macrophages using Affymetrix Mouse Genome arrays to identify genes predominantly expressed by microglia. Among tens of thousands of genes assayed, 127 potential candidates were found, including nine newly discovered genes encoding plasma membrane and extracellular proteins. In the brain, the latter were selectively expressed by microglia, as revealed by in situ hybridization. Three of them were confirmed to be exclusively (MSR2) or predominantly (GPR12, GPR34) expressed in the brain compared to the other tissues examined. Furthermore, all of these genes were upregulated in activated microglia after treatment with the demyelinating toxin cuprizone, suggesting that they play roles in neuroinflammation. In conclusion, this study reports the identification of new selective markers for microglia, which should prove useful not only to identify and isolate these cells, but also to better understand their distinctive properties. © 2007 Wiley-Liss, Inc.
Fil: Bédard, Andréanne. Laval University; Canadá
Fil: Tremblay, Pierrot. Laval University; Canadá
Fil: Chernomoretz, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laval University; Canadá
Fil: Vallières, Luc. Laval University; Canadá
description Microglia, monocytes, and peripheral macrophages share a common origin and many characteristics, but what distinguishes them from each other at the level of gene expression remains largely unknown. In this study, we compared the transcriptional profiles of freshly purified microglia, monocytes, and spleen macrophages using Affymetrix Mouse Genome arrays to identify genes predominantly expressed by microglia. Among tens of thousands of genes assayed, 127 potential candidates were found, including nine newly discovered genes encoding plasma membrane and extracellular proteins. In the brain, the latter were selectively expressed by microglia, as revealed by in situ hybridization. Three of them were confirmed to be exclusively (MSR2) or predominantly (GPR12, GPR34) expressed in the brain compared to the other tissues examined. Furthermore, all of these genes were upregulated in activated microglia after treatment with the demyelinating toxin cuprizone, suggesting that they play roles in neuroinflammation. In conclusion, this study reports the identification of new selective markers for microglia, which should prove useful not only to identify and isolate these cells, but also to better understand their distinctive properties. © 2007 Wiley-Liss, Inc.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/70606
Bédard, Andréanne; Tremblay, Pierrot; Chernomoretz, Ariel; Vallières, Luc; Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation; Wiley-liss, Div John Wiley & Sons Inc; Glia; 55; 8; 6-2007; 777-789
0894-1491
CONICET Digital
CONICET
url http://hdl.handle.net/11336/70606
identifier_str_mv Bédard, Andréanne; Tremblay, Pierrot; Chernomoretz, Ariel; Vallières, Luc; Identification of genes preferentially expressed by microglia and upregulated during cuprizone-induced inflammation; Wiley-liss, Div John Wiley & Sons Inc; Glia; 55; 8; 6-2007; 777-789
0894-1491
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/glia.20477
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.20477
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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