Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained
- Autores
- Fenoglio, Cecilia Lorena; Vierling, Noelia; Manzo, Ricardo Martín; Ceruti, Roberto Julio; Sihufe, Guillermo Adrian; Mammarella, Enrique José
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this work, the effects of operating parameters on the modulation of peptide profiles obtained during enzymatic hydrolysis of whey protein concentrates (WPC) with a commercial enzyme (Alcalase 2.4L AF) were analyzed. Hydrolyzates of WPC were obtained working with free and immobilized enzyme and evaluating different operating conditions (initial substrate concentrations, time, temperature and pH). Characteristics of hydrolyzates obtained were evaluated by the hydrolysis degree obtained (DH) and the peptide profile by RP-HPLC. Moreover, a quantitative descriptive sensory analysis was performed to compare different preparations of commercial desserts prepared using as ingredients milk, WPC and WPC hydrolyzates. The DH values increased very quickly during the first 10 min. followed by a slow increase up to 25 min. For the range of conditions tested, we proposed to operate at temperatures close to 50˚C, pH between 8 and 9 and initial substrate concentration from 7% as good reactions conditions. Finally, whey protein hydrolyzates obtained with Alcalase showed very interesting sensorial characteristics to be incorporated in desserts commercial preparations.
Fil: Fenoglio, Cecilia Lorena. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vierling, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Ceruti, Roberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Sihufe, Guillermo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina - Materia
-
milk proteins
WPC
enzymatic hydrolysis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/23809
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Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtainedFenoglio, Cecilia LorenaVierling, NoeliaManzo, Ricardo MartínCeruti, Roberto JulioSihufe, Guillermo AdrianMammarella, Enrique Josémilk proteinsWPCenzymatic hydrolysishttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2In this work, the effects of operating parameters on the modulation of peptide profiles obtained during enzymatic hydrolysis of whey protein concentrates (WPC) with a commercial enzyme (Alcalase 2.4L AF) were analyzed. Hydrolyzates of WPC were obtained working with free and immobilized enzyme and evaluating different operating conditions (initial substrate concentrations, time, temperature and pH). Characteristics of hydrolyzates obtained were evaluated by the hydrolysis degree obtained (DH) and the peptide profile by RP-HPLC. Moreover, a quantitative descriptive sensory analysis was performed to compare different preparations of commercial desserts prepared using as ingredients milk, WPC and WPC hydrolyzates. The DH values increased very quickly during the first 10 min. followed by a slow increase up to 25 min. For the range of conditions tested, we proposed to operate at temperatures close to 50˚C, pH between 8 and 9 and initial substrate concentration from 7% as good reactions conditions. Finally, whey protein hydrolyzates obtained with Alcalase showed very interesting sensorial characteristics to be incorporated in desserts commercial preparations.Fil: Fenoglio, Cecilia Lorena. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vierling, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Ceruti, Roberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Sihufe, Guillermo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaScience Alert2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23809Fenoglio, Cecilia Lorena; Vierling, Noelia; Manzo, Ricardo Martín; Ceruti, Roberto Julio; Sihufe, Guillermo Adrian; et al.; Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained; Science Alert; American Journal of Food Technology; 11; 4; 4-2016; 152-1581557-4571CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://scialert.net/eboard.php?issn=1557-4571info:eu-repo/semantics/altIdentifier/doi/10.3923/ajft.2016.152.158info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:52Zoai:ri.conicet.gov.ar:11336/23809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:52.563CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained |
title |
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained |
spellingShingle |
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained Fenoglio, Cecilia Lorena milk proteins WPC enzymatic hydrolysis |
title_short |
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained |
title_full |
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained |
title_fullStr |
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained |
title_full_unstemmed |
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained |
title_sort |
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained |
dc.creator.none.fl_str_mv |
Fenoglio, Cecilia Lorena Vierling, Noelia Manzo, Ricardo Martín Ceruti, Roberto Julio Sihufe, Guillermo Adrian Mammarella, Enrique José |
author |
Fenoglio, Cecilia Lorena |
author_facet |
Fenoglio, Cecilia Lorena Vierling, Noelia Manzo, Ricardo Martín Ceruti, Roberto Julio Sihufe, Guillermo Adrian Mammarella, Enrique José |
author_role |
author |
author2 |
Vierling, Noelia Manzo, Ricardo Martín Ceruti, Roberto Julio Sihufe, Guillermo Adrian Mammarella, Enrique José |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
milk proteins WPC enzymatic hydrolysis |
topic |
milk proteins WPC enzymatic hydrolysis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
In this work, the effects of operating parameters on the modulation of peptide profiles obtained during enzymatic hydrolysis of whey protein concentrates (WPC) with a commercial enzyme (Alcalase 2.4L AF) were analyzed. Hydrolyzates of WPC were obtained working with free and immobilized enzyme and evaluating different operating conditions (initial substrate concentrations, time, temperature and pH). Characteristics of hydrolyzates obtained were evaluated by the hydrolysis degree obtained (DH) and the peptide profile by RP-HPLC. Moreover, a quantitative descriptive sensory analysis was performed to compare different preparations of commercial desserts prepared using as ingredients milk, WPC and WPC hydrolyzates. The DH values increased very quickly during the first 10 min. followed by a slow increase up to 25 min. For the range of conditions tested, we proposed to operate at temperatures close to 50˚C, pH between 8 and 9 and initial substrate concentration from 7% as good reactions conditions. Finally, whey protein hydrolyzates obtained with Alcalase showed very interesting sensorial characteristics to be incorporated in desserts commercial preparations. Fil: Fenoglio, Cecilia Lorena. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vierling, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Ceruti, Roberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Sihufe, Guillermo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina |
description |
In this work, the effects of operating parameters on the modulation of peptide profiles obtained during enzymatic hydrolysis of whey protein concentrates (WPC) with a commercial enzyme (Alcalase 2.4L AF) were analyzed. Hydrolyzates of WPC were obtained working with free and immobilized enzyme and evaluating different operating conditions (initial substrate concentrations, time, temperature and pH). Characteristics of hydrolyzates obtained were evaluated by the hydrolysis degree obtained (DH) and the peptide profile by RP-HPLC. Moreover, a quantitative descriptive sensory analysis was performed to compare different preparations of commercial desserts prepared using as ingredients milk, WPC and WPC hydrolyzates. The DH values increased very quickly during the first 10 min. followed by a slow increase up to 25 min. For the range of conditions tested, we proposed to operate at temperatures close to 50˚C, pH between 8 and 9 and initial substrate concentration from 7% as good reactions conditions. Finally, whey protein hydrolyzates obtained with Alcalase showed very interesting sensorial characteristics to be incorporated in desserts commercial preparations. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/23809 Fenoglio, Cecilia Lorena; Vierling, Noelia; Manzo, Ricardo Martín; Ceruti, Roberto Julio; Sihufe, Guillermo Adrian; et al.; Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained; Science Alert; American Journal of Food Technology; 11; 4; 4-2016; 152-158 1557-4571 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/23809 |
identifier_str_mv |
Fenoglio, Cecilia Lorena; Vierling, Noelia; Manzo, Ricardo Martín; Ceruti, Roberto Julio; Sihufe, Guillermo Adrian; et al.; Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained; Science Alert; American Journal of Food Technology; 11; 4; 4-2016; 152-158 1557-4571 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://scialert.net/eboard.php?issn=1557-4571 info:eu-repo/semantics/altIdentifier/doi/10.3923/ajft.2016.152.158 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Science Alert |
publisher.none.fl_str_mv |
Science Alert |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |