Assessing the potential of Rivularia halophila for arsenic removal

Autores
Mlewski, Estela Cecilia; Soto Rueda, Eliana; Borgnino Bianchi, Laura Carolina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Rivularia halophila (R. halophila) is a filamentous cyanobacteria isolated from microbial mats of the Laguna Negra Lake (LN), Catamarca, Puna-Argentina. The LN is a high-altitude hypersaline lake, where extreme environmental conditions (i.e., high UV-radiation and extreme temperature, salinity, and water activity) restrict eukaryotic life. Besides, the presence of arsenic (As) has been detected in water, sediments and lithified microbial mats. This particular cyanobacteria is part of a microbial consortium that participates actively in the carbonate precipitation process. Taking this into account, the objective of this study were (i) to evaluate the capability of R. halophila to tolerate moderate to high As concentration and (ii) to assess the role of this cyanobacteria in carbonate precipitation with the potential incorporation of As in the carbonate lattice. Tolerance and resistance experiments were perform under different As (III and V) concentration, and evaluated by biomass growth, pigment intensity and chlorophyll a content. Besides, lethal dose 50 (LD 50) was also evaluated. On the other hand, carbonate precipitation experiments were perform under different calcium concentrations and moderate levels of As (similar to LN concentrations). Optical and electronic microscopy images of the precipitates were taken and measured near the cyanobacterial sheaths and from the bottom of the culture flask. DRX analysis were also performed. Preliminary results showed that R. halophila tolerates high concentrations of As (III and V), especially As (V), and can accumulate As in the sheath and in the cells. Moreover, R. halophila might facilitate carbonate precipitation in culture. The precipitation of amorphous crystals, near the sheath, were observed with the addition of As (V); while without R. halophila the minerals were mostly geometric, corresponding to chemical precipitation. All the results obtained until now, give us some clues about the potential of this cyanobacteria for As bio-removal.
Fil: Mlewski, Estela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Soto Rueda, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
Fil: Borgnino Bianchi, Laura Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research and XVI Annual Meeting of the Argentinean Society for General Microbiology
Virtual
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Asociación Civil de Microbiología General
Materia
ARSENIC
CYANOBACTERIA
BIO REMOVAL
CARBONATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/165010
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Assessing the potential of Rivularia halophila for arsenic removalMlewski, Estela CeciliaSoto Rueda, ElianaBorgnino Bianchi, Laura CarolinaARSENICCYANOBACTERIABIO REMOVALCARBONATEhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Rivularia halophila (R. halophila) is a filamentous cyanobacteria isolated from microbial mats of the Laguna Negra Lake (LN), Catamarca, Puna-Argentina. The LN is a high-altitude hypersaline lake, where extreme environmental conditions (i.e., high UV-radiation and extreme temperature, salinity, and water activity) restrict eukaryotic life. Besides, the presence of arsenic (As) has been detected in water, sediments and lithified microbial mats. This particular cyanobacteria is part of a microbial consortium that participates actively in the carbonate precipitation process. Taking this into account, the objective of this study were (i) to evaluate the capability of R. halophila to tolerate moderate to high As concentration and (ii) to assess the role of this cyanobacteria in carbonate precipitation with the potential incorporation of As in the carbonate lattice. Tolerance and resistance experiments were perform under different As (III and V) concentration, and evaluated by biomass growth, pigment intensity and chlorophyll a content. Besides, lethal dose 50 (LD 50) was also evaluated. On the other hand, carbonate precipitation experiments were perform under different calcium concentrations and moderate levels of As (similar to LN concentrations). Optical and electronic microscopy images of the precipitates were taken and measured near the cyanobacterial sheaths and from the bottom of the culture flask. DRX analysis were also performed. Preliminary results showed that R. halophila tolerates high concentrations of As (III and V), especially As (V), and can accumulate As in the sheath and in the cells. Moreover, R. halophila might facilitate carbonate precipitation in culture. The precipitation of amorphous crystals, near the sheath, were observed with the addition of As (V); while without R. halophila the minerals were mostly geometric, corresponding to chemical precipitation. All the results obtained until now, give us some clues about the potential of this cyanobacteria for As bio-removal.Fil: Mlewski, Estela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Soto Rueda, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Borgnino Bianchi, Laura Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaLVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research and XVI Annual Meeting of the Argentinean Society for General MicrobiologyVirtualArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularAsociación Civil de Microbiología GeneralTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/165010Assessing the potential of Rivularia halophila for arsenic removal; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research and XVI Annual Meeting of the Argentinean Society for General Microbiology; Virtual; Argentina; 2021; 40-410327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://congresos.g2consultora.com/wp-content/uploads/2021/10/Biocell-Preprint-SAIB-SAMIGE-2021.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:55Zoai:ri.conicet.gov.ar:11336/165010instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:55.793CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessing the potential of Rivularia halophila for arsenic removal
title Assessing the potential of Rivularia halophila for arsenic removal
spellingShingle Assessing the potential of Rivularia halophila for arsenic removal
Mlewski, Estela Cecilia
ARSENIC
CYANOBACTERIA
BIO REMOVAL
CARBONATE
title_short Assessing the potential of Rivularia halophila for arsenic removal
title_full Assessing the potential of Rivularia halophila for arsenic removal
title_fullStr Assessing the potential of Rivularia halophila for arsenic removal
title_full_unstemmed Assessing the potential of Rivularia halophila for arsenic removal
title_sort Assessing the potential of Rivularia halophila for arsenic removal
dc.creator.none.fl_str_mv Mlewski, Estela Cecilia
Soto Rueda, Eliana
Borgnino Bianchi, Laura Carolina
author Mlewski, Estela Cecilia
author_facet Mlewski, Estela Cecilia
Soto Rueda, Eliana
Borgnino Bianchi, Laura Carolina
author_role author
author2 Soto Rueda, Eliana
Borgnino Bianchi, Laura Carolina
author2_role author
author
dc.subject.none.fl_str_mv ARSENIC
CYANOBACTERIA
BIO REMOVAL
CARBONATE
topic ARSENIC
CYANOBACTERIA
BIO REMOVAL
CARBONATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rivularia halophila (R. halophila) is a filamentous cyanobacteria isolated from microbial mats of the Laguna Negra Lake (LN), Catamarca, Puna-Argentina. The LN is a high-altitude hypersaline lake, where extreme environmental conditions (i.e., high UV-radiation and extreme temperature, salinity, and water activity) restrict eukaryotic life. Besides, the presence of arsenic (As) has been detected in water, sediments and lithified microbial mats. This particular cyanobacteria is part of a microbial consortium that participates actively in the carbonate precipitation process. Taking this into account, the objective of this study were (i) to evaluate the capability of R. halophila to tolerate moderate to high As concentration and (ii) to assess the role of this cyanobacteria in carbonate precipitation with the potential incorporation of As in the carbonate lattice. Tolerance and resistance experiments were perform under different As (III and V) concentration, and evaluated by biomass growth, pigment intensity and chlorophyll a content. Besides, lethal dose 50 (LD 50) was also evaluated. On the other hand, carbonate precipitation experiments were perform under different calcium concentrations and moderate levels of As (similar to LN concentrations). Optical and electronic microscopy images of the precipitates were taken and measured near the cyanobacterial sheaths and from the bottom of the culture flask. DRX analysis were also performed. Preliminary results showed that R. halophila tolerates high concentrations of As (III and V), especially As (V), and can accumulate As in the sheath and in the cells. Moreover, R. halophila might facilitate carbonate precipitation in culture. The precipitation of amorphous crystals, near the sheath, were observed with the addition of As (V); while without R. halophila the minerals were mostly geometric, corresponding to chemical precipitation. All the results obtained until now, give us some clues about the potential of this cyanobacteria for As bio-removal.
Fil: Mlewski, Estela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Soto Rueda, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
Fil: Borgnino Bianchi, Laura Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research and XVI Annual Meeting of the Argentinean Society for General Microbiology
Virtual
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Asociación Civil de Microbiología General
description Rivularia halophila (R. halophila) is a filamentous cyanobacteria isolated from microbial mats of the Laguna Negra Lake (LN), Catamarca, Puna-Argentina. The LN is a high-altitude hypersaline lake, where extreme environmental conditions (i.e., high UV-radiation and extreme temperature, salinity, and water activity) restrict eukaryotic life. Besides, the presence of arsenic (As) has been detected in water, sediments and lithified microbial mats. This particular cyanobacteria is part of a microbial consortium that participates actively in the carbonate precipitation process. Taking this into account, the objective of this study were (i) to evaluate the capability of R. halophila to tolerate moderate to high As concentration and (ii) to assess the role of this cyanobacteria in carbonate precipitation with the potential incorporation of As in the carbonate lattice. Tolerance and resistance experiments were perform under different As (III and V) concentration, and evaluated by biomass growth, pigment intensity and chlorophyll a content. Besides, lethal dose 50 (LD 50) was also evaluated. On the other hand, carbonate precipitation experiments were perform under different calcium concentrations and moderate levels of As (similar to LN concentrations). Optical and electronic microscopy images of the precipitates were taken and measured near the cyanobacterial sheaths and from the bottom of the culture flask. DRX analysis were also performed. Preliminary results showed that R. halophila tolerates high concentrations of As (III and V), especially As (V), and can accumulate As in the sheath and in the cells. Moreover, R. halophila might facilitate carbonate precipitation in culture. The precipitation of amorphous crystals, near the sheath, were observed with the addition of As (V); while without R. halophila the minerals were mostly geometric, corresponding to chemical precipitation. All the results obtained until now, give us some clues about the potential of this cyanobacteria for As bio-removal.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/165010
Assessing the potential of Rivularia halophila for arsenic removal; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research and XVI Annual Meeting of the Argentinean Society for General Microbiology; Virtual; Argentina; 2021; 40-41
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/165010
identifier_str_mv Assessing the potential of Rivularia halophila for arsenic removal; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research and XVI Annual Meeting of the Argentinean Society for General Microbiology; Virtual; Argentina; 2021; 40-41
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://congresos.g2consultora.com/wp-content/uploads/2021/10/Biocell-Preprint-SAIB-SAMIGE-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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