An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting

Autores
Fernández Honaine, Mariana; Benvenuto, María Laura; Osterrieth, Margarita Luisa
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and aims: Different methodologies were proposed for the detection of silica deposits in plant tissues. These methodologies include dry and wet ashing (which destroy the surrounding tissue), phenol staining (toxic), safranin-crystal violet lactone and fast green-methyl red staining (not specific for silica), fluorescence microscopy, electronic microscopy, EDAX and Raman analyses (which involve expensive equipment). Here we presented an easy and cheap method based on tissue clearing and immersion oil mounting. M & M: We tested the methodology in longitudinal and cross sections of culms, leaves and roots of ten species that effectively accumulate silica. We applied different clearing techniques according to the type of plant material, we mounted in immersion oil and observed under light microscope. We compared the results with the ones obtained by traditional silicophytolith techniques. Results: Silica deposits were observed in all species and organs analyzed, and the observations were coincident with the results obtained by other techniques. It was also possible to identify calcium crystals, allowing the description of the most common biomineralizations produced by plants. Conclusions: The technique here proposed can be used for exploratory as well as for specific studies about the content and distribution of silicophytoliths in almost any tissue, organ and plant species. It can be applied in any laboratory, because it does not require expensive or hardly available equipment.
Fil: Fernández Honaine, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Benvenuto, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Osterrieth, Margarita Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
ANATOMY
DICOTYLEDONS
EQUISETUM
INMMERSION OIL
MONOCOTYLEDONS
SILICOPHYTOLITHS
TISSUE CLEARING
INMMERSION OIL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/122600
Acceder
id CONICETDig_5a5546b5d98ac35438053917c1df1c0d
oai_identifier_str oai:ri.conicet.gov.ar:11336/122600
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mountingUna técnica sencilla para la visualización de silicofitolitos en plantas mediante clarificación y montaje en aceite de inmersiónFernández Honaine, MarianaBenvenuto, María LauraOsterrieth, Margarita LuisaANATOMYDICOTYLEDONSEQUISETUMINMMERSION OILMONOCOTYLEDONSSILICOPHYTOLITHSTISSUE CLEARINGINMMERSION OILhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background and aims: Different methodologies were proposed for the detection of silica deposits in plant tissues. These methodologies include dry and wet ashing (which destroy the surrounding tissue), phenol staining (toxic), safranin-crystal violet lactone and fast green-methyl red staining (not specific for silica), fluorescence microscopy, electronic microscopy, EDAX and Raman analyses (which involve expensive equipment). Here we presented an easy and cheap method based on tissue clearing and immersion oil mounting. M & M: We tested the methodology in longitudinal and cross sections of culms, leaves and roots of ten species that effectively accumulate silica. We applied different clearing techniques according to the type of plant material, we mounted in immersion oil and observed under light microscope. We compared the results with the ones obtained by traditional silicophytolith techniques. Results: Silica deposits were observed in all species and organs analyzed, and the observations were coincident with the results obtained by other techniques. It was also possible to identify calcium crystals, allowing the description of the most common biomineralizations produced by plants. Conclusions: The technique here proposed can be used for exploratory as well as for specific studies about the content and distribution of silicophytoliths in almost any tissue, organ and plant species. It can be applied in any laboratory, because it does not require expensive or hardly available equipment.Fil: Fernández Honaine, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Benvenuto, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Osterrieth, Margarita Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaSociedad Argentina de Botánica2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122600Fernández Honaine, Mariana; Benvenuto, María Laura; Osterrieth, Margarita Luisa; An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting; Sociedad Argentina de Botánica; Boletín de la Sociedad Argentina de Botánica; 54; 3; 9-2019; 353-3650373-580X1851-2372CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://botanicaargentina.org.ar/an-easy-technique-for-silicophytolith-visualization-in-plants-through-tissue-clearing-and-immersion-oil-mounting/info:eu-repo/semantics/altIdentifier/doi/10.31055/1851.2372.v54.n3.25359info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:16:39Zoai:ri.conicet.gov.ar:11336/122600instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:16:40.075CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
Una técnica sencilla para la visualización de silicofitolitos en plantas mediante clarificación y montaje en aceite de inmersión
title An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
spellingShingle An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
Fernández Honaine, Mariana
ANATOMY
DICOTYLEDONS
EQUISETUM
INMMERSION OIL
MONOCOTYLEDONS
SILICOPHYTOLITHS
TISSUE CLEARING
INMMERSION OIL
title_short An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_full An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_fullStr An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_full_unstemmed An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_sort An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
dc.creator.none.fl_str_mv Fernández Honaine, Mariana
Benvenuto, María Laura
Osterrieth, Margarita Luisa
author Fernández Honaine, Mariana
author_facet Fernández Honaine, Mariana
Benvenuto, María Laura
Osterrieth, Margarita Luisa
author_role author
author2 Benvenuto, María Laura
Osterrieth, Margarita Luisa
author2_role author
author
dc.subject.none.fl_str_mv ANATOMY
DICOTYLEDONS
EQUISETUM
INMMERSION OIL
MONOCOTYLEDONS
SILICOPHYTOLITHS
TISSUE CLEARING
INMMERSION OIL
topic ANATOMY
DICOTYLEDONS
EQUISETUM
INMMERSION OIL
MONOCOTYLEDONS
SILICOPHYTOLITHS
TISSUE CLEARING
INMMERSION OIL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background and aims: Different methodologies were proposed for the detection of silica deposits in plant tissues. These methodologies include dry and wet ashing (which destroy the surrounding tissue), phenol staining (toxic), safranin-crystal violet lactone and fast green-methyl red staining (not specific for silica), fluorescence microscopy, electronic microscopy, EDAX and Raman analyses (which involve expensive equipment). Here we presented an easy and cheap method based on tissue clearing and immersion oil mounting. M & M: We tested the methodology in longitudinal and cross sections of culms, leaves and roots of ten species that effectively accumulate silica. We applied different clearing techniques according to the type of plant material, we mounted in immersion oil and observed under light microscope. We compared the results with the ones obtained by traditional silicophytolith techniques. Results: Silica deposits were observed in all species and organs analyzed, and the observations were coincident with the results obtained by other techniques. It was also possible to identify calcium crystals, allowing the description of the most common biomineralizations produced by plants. Conclusions: The technique here proposed can be used for exploratory as well as for specific studies about the content and distribution of silicophytoliths in almost any tissue, organ and plant species. It can be applied in any laboratory, because it does not require expensive or hardly available equipment.
Fil: Fernández Honaine, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Benvenuto, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Osterrieth, Margarita Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description Background and aims: Different methodologies were proposed for the detection of silica deposits in plant tissues. These methodologies include dry and wet ashing (which destroy the surrounding tissue), phenol staining (toxic), safranin-crystal violet lactone and fast green-methyl red staining (not specific for silica), fluorescence microscopy, electronic microscopy, EDAX and Raman analyses (which involve expensive equipment). Here we presented an easy and cheap method based on tissue clearing and immersion oil mounting. M & M: We tested the methodology in longitudinal and cross sections of culms, leaves and roots of ten species that effectively accumulate silica. We applied different clearing techniques according to the type of plant material, we mounted in immersion oil and observed under light microscope. We compared the results with the ones obtained by traditional silicophytolith techniques. Results: Silica deposits were observed in all species and organs analyzed, and the observations were coincident with the results obtained by other techniques. It was also possible to identify calcium crystals, allowing the description of the most common biomineralizations produced by plants. Conclusions: The technique here proposed can be used for exploratory as well as for specific studies about the content and distribution of silicophytoliths in almost any tissue, organ and plant species. It can be applied in any laboratory, because it does not require expensive or hardly available equipment.
publishDate 2019
dc.date.none.fl_str_mv 2019-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/122600
Fernández Honaine, Mariana; Benvenuto, María Laura; Osterrieth, Margarita Luisa; An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting; Sociedad Argentina de Botánica; Boletín de la Sociedad Argentina de Botánica; 54; 3; 9-2019; 353-365
0373-580X
1851-2372
CONICET Digital
CONICET
url http://hdl.handle.net/11336/122600
identifier_str_mv Fernández Honaine, Mariana; Benvenuto, María Laura; Osterrieth, Margarita Luisa; An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting; Sociedad Argentina de Botánica; Boletín de la Sociedad Argentina de Botánica; 54; 3; 9-2019; 353-365
0373-580X
1851-2372
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://botanicaargentina.org.ar/an-easy-technique-for-silicophytolith-visualization-in-plants-through-tissue-clearing-and-immersion-oil-mounting/
info:eu-repo/semantics/altIdentifier/doi/10.31055/1851.2372.v54.n3.25359
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Botánica
publisher.none.fl_str_mv Sociedad Argentina de Botánica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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