Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy

Autores
Superina, Mariella; Morales, Melisa Estefanía
Año de publicación
2023
Idioma
español castellano
Tipo de recurso
conjunto de datos
Estado
Descripción
The transmission cycles of Trypanosoma cruzi, the causative agent of Chagas disease, include a wide variety of mammals and haematophagous[MS1]  triatomine insects. Infection with this blood parasite has been confirmed in many armadillo species; however, information on infection in Zaedyus pichiy, a small armadillo that inhabits areas endemic to Chagas disease, is scarce. Our objective was to determine the infection frequency and parasite load of T. cruzi in 49 wild Z. pichiy confiscated dead from poachers in Mendoza, Argentina, 2010-2017. We detected T. cruzi DNA in 32 of 49 armadillos (65%) using real-time PCR, confirming infection with T. cruzi in a high proportion of confiscated pichis. No differences were found related to sex, age, or ecoregion origin of the assessed pichis. Parasite loads ranged between <0.1-8[MS2] .88 parasite-equivalents/µg [MS3] cardiac tissue. Additional studies on the infection status of Z. pichiy are needed to determine their role in the maintenance of the sylvatic transmission cycle and the potential zoonotic risk from hunted pichis.
Fil: Superina, Mariella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Morales, Melisa Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Protección de datos personales (Ley 25.326)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192665
Acceder
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spelling Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiySuperina, MariellaMorales, Melisa Estefaníahttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The transmission cycles of Trypanosoma cruzi, the causative agent of Chagas disease, include a wide variety of mammals and haematophagous[MS1]  triatomine insects. Infection with this blood parasite has been confirmed in many armadillo species; however, information on infection in Zaedyus pichiy, a small armadillo that inhabits areas endemic to Chagas disease, is scarce. Our objective was to determine the infection frequency and parasite load of T. cruzi in 49 wild Z. pichiy confiscated dead from poachers in Mendoza, Argentina, 2010-2017. We detected T. cruzi DNA in 32 of 49 armadillos (65%) using real-time PCR, confirming infection with T. cruzi in a high proportion of confiscated pichis. No differences were found related to sex, age, or ecoregion origin of the assessed pichis. Parasite loads ranged between <0.1-8[MS2] .88 parasite-equivalents/µg [MS3] cardiac tissue. Additional studies on the infection status of Z. pichiy are needed to determine their role in the maintenance of the sylvatic transmission cycle and the potential zoonotic risk from hunted pichis.Fil: Superina, Mariella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Morales, Melisa Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina2023info:ar-repo/semantics/conjuntoDeDatosv1.0info:eu-repo/semantics/dataSetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://hdl.handle.net/11336/192665Superina, Mariella; Morales, Melisa Estefanía; (2023): Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/192665CONICET DigitalCONICETspainfo:eu-repo/grantAgreement/Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica/PICT 2014-0496info:eu-repo/grantAgreement/Fundacion Bunge y Born./PICT 2014-0496info:eu-repo/semantics/restrictedAccessProtección de datos personales (Ley 25.326)reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:38:49Zoai:ri.conicet.gov.ar:11336/192665instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:38:49.535CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy
title Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy
spellingShingle Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy
Superina, Mariella
title_short Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy
title_full Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy
title_fullStr Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy
title_full_unstemmed Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy
title_sort Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy
dc.creator.none.fl_str_mv Superina, Mariella
Morales, Melisa Estefanía
author Superina, Mariella
author_facet Superina, Mariella
Morales, Melisa Estefanía
author_role author
author2 Morales, Melisa Estefanía
author2_role author
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The transmission cycles of Trypanosoma cruzi, the causative agent of Chagas disease, include a wide variety of mammals and haematophagous[MS1]  triatomine insects. Infection with this blood parasite has been confirmed in many armadillo species; however, information on infection in Zaedyus pichiy, a small armadillo that inhabits areas endemic to Chagas disease, is scarce. Our objective was to determine the infection frequency and parasite load of T. cruzi in 49 wild Z. pichiy confiscated dead from poachers in Mendoza, Argentina, 2010-2017. We detected T. cruzi DNA in 32 of 49 armadillos (65%) using real-time PCR, confirming infection with T. cruzi in a high proportion of confiscated pichis. No differences were found related to sex, age, or ecoregion origin of the assessed pichis. Parasite loads ranged between <0.1-8[MS2] .88 parasite-equivalents/µg [MS3] cardiac tissue. Additional studies on the infection status of Z. pichiy are needed to determine their role in the maintenance of the sylvatic transmission cycle and the potential zoonotic risk from hunted pichis.
Fil: Superina, Mariella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Morales, Melisa Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description The transmission cycles of Trypanosoma cruzi, the causative agent of Chagas disease, include a wide variety of mammals and haematophagous[MS1]  triatomine insects. Infection with this blood parasite has been confirmed in many armadillo species; however, information on infection in Zaedyus pichiy, a small armadillo that inhabits areas endemic to Chagas disease, is scarce. Our objective was to determine the infection frequency and parasite load of T. cruzi in 49 wild Z. pichiy confiscated dead from poachers in Mendoza, Argentina, 2010-2017. We detected T. cruzi DNA in 32 of 49 armadillos (65%) using real-time PCR, confirming infection with T. cruzi in a high proportion of confiscated pichis. No differences were found related to sex, age, or ecoregion origin of the assessed pichis. Parasite loads ranged between <0.1-8[MS2] .88 parasite-equivalents/µg [MS3] cardiac tissue. Additional studies on the infection status of Z. pichiy are needed to determine their role in the maintenance of the sylvatic transmission cycle and the potential zoonotic risk from hunted pichis.
publishDate 2023
dc.date.none.fl_str_mv 2023
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v1.0
info:eu-repo/semantics/dataSet
format dataSet
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192665
Superina, Mariella; Morales, Melisa Estefanía; (2023): Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/192665
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192665
identifier_str_mv Superina, Mariella; Morales, Melisa Estefanía; (2023): Análisis molecular de Trypanosoma cruzi en muestras de corazones de Zaedyus pichiy. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/192665
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
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info:eu-repo/grantAgreement/Fundacion Bunge y Born./PICT 2014-0496
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Protección de datos personales (Ley 25.326)
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rights_invalid_str_mv Protección de datos personales (Ley 25.326)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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