Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients

Autores
Guzmán Mantesana, Gustavo; Báez, Alejandra L.; Lo Presti, Maria Silvina; Domínguez, Ricardo; Córdoba, Roque; Bazán, Carolina; Strauss, Mariana; Fretes, Ricardo; Rivarola, Héctor W.; Paglini Oliva, Patricia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and Aims: The fundamental mechanisms involved in the genesis and progression of heart failure are not clearly understood. The present study was conducted to analyze the cardiac mitochondrial involvement in heart failure, the possible parallelism between cardiac and skeletal muscle and if there is a link between clinical symptoms and mitochondrial damage. Methods: Left ventricle and pectoral biopsies were obtained from patients with heart failure (n: 21) and patients with inter-auricular communication as the unique diagnosis for surgery (n: 6). Mitochondria were isolated from these tissues and studied through electron microscopy, spectrophotometry to measure the activity of respiratory complex III and immunohistochemistry to determine the presence of reactive oxygen species. Results: More than 90% of cardiac and skeletal muscle mitochondria presented structural and functional alterations in relation to an increment in the reactive oxygen species production, even in patients without the presence of any clinical Framingham criteria. Conclusions: We demonstrated some parallelism between cardiac and skeletal muscle mitochondrial alterations in patients with heart failure and that these alterations begin before the major clinical Framingham criteria are installed, pointing to mitochondria as one of the possibly responsible factors for the evolution of cardiac disease.
Fil: Guzmán Mantesana, Gustavo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Báez, Alejandra L.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Lo Presti, Maria Silvina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Domínguez, Ricardo. Instituto del Corazón de La Rioja; Argentina
Fil: Córdoba, Roque. Clínica Allende; Argentina
Fil: Bazán, Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Strauss, Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Fretes, Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Rivarola, Héctor W.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Paglini Oliva, Patricia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Materia
Mitochondria
Heart Failure
Functional And Structural Alterations
Cardiac And Skeletal Muscle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11514
Acceder
id CONICETDig_5a0d7f8ca1b302d3b59a486e8f19a09a
oai_identifier_str oai:ri.conicet.gov.ar:11336/11514
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patientsGuzmán Mantesana, GustavoBáez, Alejandra L.Lo Presti, Maria SilvinaDomínguez, RicardoCórdoba, RoqueBazán, CarolinaStrauss, MarianaFretes, RicardoRivarola, Héctor W.Paglini Oliva, PatriciaMitochondriaHeart FailureFunctional And Structural AlterationsCardiac And Skeletal Musclehttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background and Aims: The fundamental mechanisms involved in the genesis and progression of heart failure are not clearly understood. The present study was conducted to analyze the cardiac mitochondrial involvement in heart failure, the possible parallelism between cardiac and skeletal muscle and if there is a link between clinical symptoms and mitochondrial damage. Methods: Left ventricle and pectoral biopsies were obtained from patients with heart failure (n: 21) and patients with inter-auricular communication as the unique diagnosis for surgery (n: 6). Mitochondria were isolated from these tissues and studied through electron microscopy, spectrophotometry to measure the activity of respiratory complex III and immunohistochemistry to determine the presence of reactive oxygen species. Results: More than 90% of cardiac and skeletal muscle mitochondria presented structural and functional alterations in relation to an increment in the reactive oxygen species production, even in patients without the presence of any clinical Framingham criteria. Conclusions: We demonstrated some parallelism between cardiac and skeletal muscle mitochondrial alterations in patients with heart failure and that these alterations begin before the major clinical Framingham criteria are installed, pointing to mitochondria as one of the possibly responsible factors for the evolution of cardiac disease.Fil: Guzmán Mantesana, Gustavo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Báez, Alejandra L.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Lo Presti, Maria Silvina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Domínguez, Ricardo. Instituto del Corazón de La Rioja; ArgentinaFil: Córdoba, Roque. Clínica Allende; ArgentinaFil: Bazán, Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Strauss, Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Fretes, Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Rivarola, Héctor W.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaFil: Paglini Oliva, Patricia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; ArgentinaElsevier Inc2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11514Guzmán Mantesana, Gustavo; Báez, Alejandra L.; Lo Presti, Maria Silvina; Domínguez, Ricardo; Córdoba, Roque; et al.; Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients; Elsevier Inc; Archives Of Medical Research (eletronico); 45; 3; 4-2014; 237–2460188-4409enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.arcmed.2014.03.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0188440914000411info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:37:50Zoai:ri.conicet.gov.ar:11336/11514instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:37:50.612CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients
title Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients
spellingShingle Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients
Guzmán Mantesana, Gustavo
Mitochondria
Heart Failure
Functional And Structural Alterations
Cardiac And Skeletal Muscle
title_short Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients
title_full Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients
title_fullStr Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients
title_full_unstemmed Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients
title_sort Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients
dc.creator.none.fl_str_mv Guzmán Mantesana, Gustavo
Báez, Alejandra L.
Lo Presti, Maria Silvina
Domínguez, Ricardo
Córdoba, Roque
Bazán, Carolina
Strauss, Mariana
Fretes, Ricardo
Rivarola, Héctor W.
Paglini Oliva, Patricia
author Guzmán Mantesana, Gustavo
author_facet Guzmán Mantesana, Gustavo
Báez, Alejandra L.
Lo Presti, Maria Silvina
Domínguez, Ricardo
Córdoba, Roque
Bazán, Carolina
Strauss, Mariana
Fretes, Ricardo
Rivarola, Héctor W.
Paglini Oliva, Patricia
author_role author
author2 Báez, Alejandra L.
Lo Presti, Maria Silvina
Domínguez, Ricardo
Córdoba, Roque
Bazán, Carolina
Strauss, Mariana
Fretes, Ricardo
Rivarola, Héctor W.
Paglini Oliva, Patricia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mitochondria
Heart Failure
Functional And Structural Alterations
Cardiac And Skeletal Muscle
topic Mitochondria
Heart Failure
Functional And Structural Alterations
Cardiac And Skeletal Muscle
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background and Aims: The fundamental mechanisms involved in the genesis and progression of heart failure are not clearly understood. The present study was conducted to analyze the cardiac mitochondrial involvement in heart failure, the possible parallelism between cardiac and skeletal muscle and if there is a link between clinical symptoms and mitochondrial damage. Methods: Left ventricle and pectoral biopsies were obtained from patients with heart failure (n: 21) and patients with inter-auricular communication as the unique diagnosis for surgery (n: 6). Mitochondria were isolated from these tissues and studied through electron microscopy, spectrophotometry to measure the activity of respiratory complex III and immunohistochemistry to determine the presence of reactive oxygen species. Results: More than 90% of cardiac and skeletal muscle mitochondria presented structural and functional alterations in relation to an increment in the reactive oxygen species production, even in patients without the presence of any clinical Framingham criteria. Conclusions: We demonstrated some parallelism between cardiac and skeletal muscle mitochondrial alterations in patients with heart failure and that these alterations begin before the major clinical Framingham criteria are installed, pointing to mitochondria as one of the possibly responsible factors for the evolution of cardiac disease.
Fil: Guzmán Mantesana, Gustavo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Báez, Alejandra L.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Lo Presti, Maria Silvina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Domínguez, Ricardo. Instituto del Corazón de La Rioja; Argentina
Fil: Córdoba, Roque. Clínica Allende; Argentina
Fil: Bazán, Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Strauss, Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Fretes, Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Rivarola, Héctor W.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
Fil: Paglini Oliva, Patricia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Departamento de Fisiologia Humana y Fisica Biomedica. Catedra de Fisica Biomedica; Argentina
description Background and Aims: The fundamental mechanisms involved in the genesis and progression of heart failure are not clearly understood. The present study was conducted to analyze the cardiac mitochondrial involvement in heart failure, the possible parallelism between cardiac and skeletal muscle and if there is a link between clinical symptoms and mitochondrial damage. Methods: Left ventricle and pectoral biopsies were obtained from patients with heart failure (n: 21) and patients with inter-auricular communication as the unique diagnosis for surgery (n: 6). Mitochondria were isolated from these tissues and studied through electron microscopy, spectrophotometry to measure the activity of respiratory complex III and immunohistochemistry to determine the presence of reactive oxygen species. Results: More than 90% of cardiac and skeletal muscle mitochondria presented structural and functional alterations in relation to an increment in the reactive oxygen species production, even in patients without the presence of any clinical Framingham criteria. Conclusions: We demonstrated some parallelism between cardiac and skeletal muscle mitochondrial alterations in patients with heart failure and that these alterations begin before the major clinical Framingham criteria are installed, pointing to mitochondria as one of the possibly responsible factors for the evolution of cardiac disease.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11514
Guzmán Mantesana, Gustavo; Báez, Alejandra L.; Lo Presti, Maria Silvina; Domínguez, Ricardo; Córdoba, Roque; et al.; Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients; Elsevier Inc; Archives Of Medical Research (eletronico); 45; 3; 4-2014; 237–246
0188-4409
url http://hdl.handle.net/11336/11514
identifier_str_mv Guzmán Mantesana, Gustavo; Báez, Alejandra L.; Lo Presti, Maria Silvina; Domínguez, Ricardo; Córdoba, Roque; et al.; Functional and structural alterations of cardiac and skeletal muscle mitochondria in heart failure patients; Elsevier Inc; Archives Of Medical Research (eletronico); 45; 3; 4-2014; 237–246
0188-4409
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.arcmed.2014.03.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0188440914000411
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc
publisher.none.fl_str_mv Elsevier Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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