Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays

Autores
Colombetti, Patricia Laura; Gonzalez, Patricia; Almeida, César Américo; Pérez Iglesias, Juan Manuel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Anthropic activities such as industrial, agricultural and urban activity can be a source of pollution gradients being proposed as a cause of habitat fragmentation. The Conlara River located in the northeast of San Luis province suffers different environmental pressures along its course, from urban to agro-industrial areas. Ourprevious work has shown that the presence of environmental stressors in the watershed negatively affects native fish. In this context, the objective of this work was to develop enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays. Sampling was carried out infour sites along the basin from S1 (upstream) to S4 (downstream). Water and sediment samples were taken from each site to carry out the bioassays on the bioindicator H. curvispina. Sediment samples were transferred to the laboratory and subsequently used for acute sediment bioassays (96 h exposure). In the samples, heavy metals and physicochemical parameters were determined. Biochemical parameters such as catalase (CAT), cholinesterase (ChE) and lipid peroxidation (TBARS) were evaluated after 96h of exposure to the sediments. For enzymatic determinations, 500 mg of H. curvispira was taken and 500uL of phosphate buffer was added. Then, they were homogenized and centrifuged at 10000 rpm for 12 min. The supernatant was then extracted for enzymatic determinations. Kruskall Wallis no parametric test was used to detect differences among sites. The biomarkers showed no significant differences in ChE (p=0,20) and CAT (p=0,20) after acute exposure, while in TBARS (p=0,10) enzimatic activity showed a gradient from site 1 (minor contamination) towards site 4 (higher contamination), indicating a stressful situation at the impacted site. Although the results showed no significant differences, there was a trend, probably due to the low number of replicates, suggesting an increase in the number of replicates in future test. It should also be noted that the samples were within the protein calibration curve, without the need for prior dilution of the amphipod pool. It is important to mention that these results are the first in semi-arid regions. Finally, although it is necessary to deepen these methodologies applied to native species of semi-arid zones, we reinforce the use of biochemical biomarkers in their ability to provide an early warning system, at the organismal level, before changes occur at the level of ecological organization.
Fil: Colombetti, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Gonzalez, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pérez Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
SETAC Latin America 15th Biennial Meeting
Montevideo, Uruguay
Uruguay
Society of Environmental Toxicology and Chemistry LatinAmerica
Materia
HYALELLA CURVISPINA
BIOMARKER
SEDIMENT BIOASSAYS
ENZYMATIC ENDPOINTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267518
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassaysColombetti, Patricia LauraGonzalez, PatriciaAlmeida, César AméricoPérez Iglesias, Juan ManuelHYALELLA CURVISPINABIOMARKERSEDIMENT BIOASSAYSENZYMATIC ENDPOINTShttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Anthropic activities such as industrial, agricultural and urban activity can be a source of pollution gradients being proposed as a cause of habitat fragmentation. The Conlara River located in the northeast of San Luis province suffers different environmental pressures along its course, from urban to agro-industrial areas. Ourprevious work has shown that the presence of environmental stressors in the watershed negatively affects native fish. In this context, the objective of this work was to develop enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays. Sampling was carried out infour sites along the basin from S1 (upstream) to S4 (downstream). Water and sediment samples were taken from each site to carry out the bioassays on the bioindicator H. curvispina. Sediment samples were transferred to the laboratory and subsequently used for acute sediment bioassays (96 h exposure). In the samples, heavy metals and physicochemical parameters were determined. Biochemical parameters such as catalase (CAT), cholinesterase (ChE) and lipid peroxidation (TBARS) were evaluated after 96h of exposure to the sediments. For enzymatic determinations, 500 mg of H. curvispira was taken and 500uL of phosphate buffer was added. Then, they were homogenized and centrifuged at 10000 rpm for 12 min. The supernatant was then extracted for enzymatic determinations. Kruskall Wallis no parametric test was used to detect differences among sites. The biomarkers showed no significant differences in ChE (p=0,20) and CAT (p=0,20) after acute exposure, while in TBARS (p=0,10) enzimatic activity showed a gradient from site 1 (minor contamination) towards site 4 (higher contamination), indicating a stressful situation at the impacted site. Although the results showed no significant differences, there was a trend, probably due to the low number of replicates, suggesting an increase in the number of replicates in future test. It should also be noted that the samples were within the protein calibration curve, without the need for prior dilution of the amphipod pool. It is important to mention that these results are the first in semi-arid regions. Finally, although it is necessary to deepen these methodologies applied to native species of semi-arid zones, we reinforce the use of biochemical biomarkers in their ability to provide an early warning system, at the organismal level, before changes occur at the level of ecological organization.Fil: Colombetti, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Gonzalez, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Pérez Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaSETAC Latin America 15th Biennial MeetingMontevideo, UruguayUruguaySociety of Environmental Toxicology and Chemistry LatinAmericaSociety of Environmental Toxicology and Chemistry2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267518Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays; SETAC Latin America 15th Biennial Meeting; Montevideo, Uruguay; Uruguay; 2023; 432-432CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.setac.org/discover-events/global-meetings/sla-15th-biennial-meeting.htmlInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:34Zoai:ri.conicet.gov.ar:11336/267518instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:35.008CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays
title Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays
spellingShingle Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays
Colombetti, Patricia Laura
HYALELLA CURVISPINA
BIOMARKER
SEDIMENT BIOASSAYS
ENZYMATIC ENDPOINTS
title_short Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays
title_full Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays
title_fullStr Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays
title_full_unstemmed Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays
title_sort Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays
dc.creator.none.fl_str_mv Colombetti, Patricia Laura
Gonzalez, Patricia
Almeida, César Américo
Pérez Iglesias, Juan Manuel
author Colombetti, Patricia Laura
author_facet Colombetti, Patricia Laura
Gonzalez, Patricia
Almeida, César Américo
Pérez Iglesias, Juan Manuel
author_role author
author2 Gonzalez, Patricia
Almeida, César Américo
Pérez Iglesias, Juan Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv HYALELLA CURVISPINA
BIOMARKER
SEDIMENT BIOASSAYS
ENZYMATIC ENDPOINTS
topic HYALELLA CURVISPINA
BIOMARKER
SEDIMENT BIOASSAYS
ENZYMATIC ENDPOINTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Anthropic activities such as industrial, agricultural and urban activity can be a source of pollution gradients being proposed as a cause of habitat fragmentation. The Conlara River located in the northeast of San Luis province suffers different environmental pressures along its course, from urban to agro-industrial areas. Ourprevious work has shown that the presence of environmental stressors in the watershed negatively affects native fish. In this context, the objective of this work was to develop enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays. Sampling was carried out infour sites along the basin from S1 (upstream) to S4 (downstream). Water and sediment samples were taken from each site to carry out the bioassays on the bioindicator H. curvispina. Sediment samples were transferred to the laboratory and subsequently used for acute sediment bioassays (96 h exposure). In the samples, heavy metals and physicochemical parameters were determined. Biochemical parameters such as catalase (CAT), cholinesterase (ChE) and lipid peroxidation (TBARS) were evaluated after 96h of exposure to the sediments. For enzymatic determinations, 500 mg of H. curvispira was taken and 500uL of phosphate buffer was added. Then, they were homogenized and centrifuged at 10000 rpm for 12 min. The supernatant was then extracted for enzymatic determinations. Kruskall Wallis no parametric test was used to detect differences among sites. The biomarkers showed no significant differences in ChE (p=0,20) and CAT (p=0,20) after acute exposure, while in TBARS (p=0,10) enzimatic activity showed a gradient from site 1 (minor contamination) towards site 4 (higher contamination), indicating a stressful situation at the impacted site. Although the results showed no significant differences, there was a trend, probably due to the low number of replicates, suggesting an increase in the number of replicates in future test. It should also be noted that the samples were within the protein calibration curve, without the need for prior dilution of the amphipod pool. It is important to mention that these results are the first in semi-arid regions. Finally, although it is necessary to deepen these methodologies applied to native species of semi-arid zones, we reinforce the use of biochemical biomarkers in their ability to provide an early warning system, at the organismal level, before changes occur at the level of ecological organization.
Fil: Colombetti, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Gonzalez, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pérez Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
SETAC Latin America 15th Biennial Meeting
Montevideo, Uruguay
Uruguay
Society of Environmental Toxicology and Chemistry LatinAmerica
description Anthropic activities such as industrial, agricultural and urban activity can be a source of pollution gradients being proposed as a cause of habitat fragmentation. The Conlara River located in the northeast of San Luis province suffers different environmental pressures along its course, from urban to agro-industrial areas. Ourprevious work has shown that the presence of environmental stressors in the watershed negatively affects native fish. In this context, the objective of this work was to develop enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays. Sampling was carried out infour sites along the basin from S1 (upstream) to S4 (downstream). Water and sediment samples were taken from each site to carry out the bioassays on the bioindicator H. curvispina. Sediment samples were transferred to the laboratory and subsequently used for acute sediment bioassays (96 h exposure). In the samples, heavy metals and physicochemical parameters were determined. Biochemical parameters such as catalase (CAT), cholinesterase (ChE) and lipid peroxidation (TBARS) were evaluated after 96h of exposure to the sediments. For enzymatic determinations, 500 mg of H. curvispira was taken and 500uL of phosphate buffer was added. Then, they were homogenized and centrifuged at 10000 rpm for 12 min. The supernatant was then extracted for enzymatic determinations. Kruskall Wallis no parametric test was used to detect differences among sites. The biomarkers showed no significant differences in ChE (p=0,20) and CAT (p=0,20) after acute exposure, while in TBARS (p=0,10) enzimatic activity showed a gradient from site 1 (minor contamination) towards site 4 (higher contamination), indicating a stressful situation at the impacted site. Although the results showed no significant differences, there was a trend, probably due to the low number of replicates, suggesting an increase in the number of replicates in future test. It should also be noted that the samples were within the protein calibration curve, without the need for prior dilution of the amphipod pool. It is important to mention that these results are the first in semi-arid regions. Finally, although it is necessary to deepen these methodologies applied to native species of semi-arid zones, we reinforce the use of biochemical biomarkers in their ability to provide an early warning system, at the organismal level, before changes occur at the level of ecological organization.
publishDate 2023
dc.date.none.fl_str_mv 2023
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status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267518
Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays; SETAC Latin America 15th Biennial Meeting; Montevideo, Uruguay; Uruguay; 2023; 432-432
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267518
identifier_str_mv Development of enzymatic endpoints in Hyalella curvispina as future biomarkers in evaluations with acute sediment bioassays; SETAC Latin America 15th Biennial Meeting; Montevideo, Uruguay; Uruguay; 2023; 432-432
CONICET Digital
CONICET
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Society of Environmental Toxicology and Chemistry
publisher.none.fl_str_mv Society of Environmental Toxicology and Chemistry
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