Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli

Autores
Dalla Via, Maria Virginia; Narduzzi, Candela; Zanetti, María Eugenia; Aguilar, Orlando Mario; Blanco, Flavio Antonio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Establishment of nitrogen fixing symbiosis requires the recognition of rhizobial molecules to initiate the development of nodules. Using transcriptional profiling of roots inoculated with mutant strains defective in the synthesis of Nod Factor (NF), exopolysaccharide (EPS) or lipopolysaccharide (LPS), we identified 2606 genes from common bean (Phaseolus vulgaris) that are differentially regulated at early stages of its interaction with Rhizobium etli. Many transcription factors from different families are modulated by NF, EPS and LPS in different combinations, suggesting that the plant response depends on the integration of multiple signals. Some receptors identified as differentially expressed constitute excellent candidates to participate in signal perception of molecules derived from the bacteria. Several components of the ethylene signal response, a hormone that plays a negative role during early stages of the process, were down-regulated by NF and LPS. In addition, genes encoding proteins involved in small RNA-mediated gene regulation were regulated by these signal molecules, such as Argonaute 7, a specific component of the TAS3 derived tasiRNAs biosynthetic pathway, an RNA dependent RNA polymerase and a XH/XP domain-containing protein, which is part of the RNA directed-DNA methylation. Interestingly, a number of genes encoding components of the circadian central oscillator were down-regulated by NF and LPS, suggesting that a root circadian clock is adjusted at early stages of symbiosis. Our results reveal a complex interaction of the responses triggered by NF, LPS and EPS that integrates information of the signals present in the surface or secreted by rhizobia.
Fil: Dalla Via, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Narduzzi, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
SYMBIOSIS
TRANSCRIPTOME
LIPOPOLYSACCHARIDES
EXOPOLYSACCHARIDE
NOD FACTOR
SIGNAL PERCEPTION AND INTEGRATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48106
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48106
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etliDalla Via, Maria VirginiaNarduzzi, CandelaZanetti, María EugeniaAguilar, Orlando MarioBlanco, Flavio AntonioSYMBIOSISTRANSCRIPTOMELIPOPOLYSACCHARIDESEXOPOLYSACCHARIDENOD FACTORSIGNAL PERCEPTION AND INTEGRATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Establishment of nitrogen fixing symbiosis requires the recognition of rhizobial molecules to initiate the development of nodules. Using transcriptional profiling of roots inoculated with mutant strains defective in the synthesis of Nod Factor (NF), exopolysaccharide (EPS) or lipopolysaccharide (LPS), we identified 2606 genes from common bean (Phaseolus vulgaris) that are differentially regulated at early stages of its interaction with Rhizobium etli. Many transcription factors from different families are modulated by NF, EPS and LPS in different combinations, suggesting that the plant response depends on the integration of multiple signals. Some receptors identified as differentially expressed constitute excellent candidates to participate in signal perception of molecules derived from the bacteria. Several components of the ethylene signal response, a hormone that plays a negative role during early stages of the process, were down-regulated by NF and LPS. In addition, genes encoding proteins involved in small RNA-mediated gene regulation were regulated by these signal molecules, such as Argonaute 7, a specific component of the TAS3 derived tasiRNAs biosynthetic pathway, an RNA dependent RNA polymerase and a XH/XP domain-containing protein, which is part of the RNA directed-DNA methylation. Interestingly, a number of genes encoding components of the circadian central oscillator were down-regulated by NF and LPS, suggesting that a root circadian clock is adjusted at early stages of symbiosis. Our results reveal a complex interaction of the responses triggered by NF, LPS and EPS that integrates information of the signals present in the surface or secreted by rhizobia.Fil: Dalla Via, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Narduzzi, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAmerican Society of Plant Biologist2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48106Dalla Via, Maria Virginia; Narduzzi, Candela; Zanetti, María Eugenia; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli; American Society of Plant Biologist; Plant Physiology; 169; 8-2015; 1356-13700032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.15.00508info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/169/2/1356info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:29Zoai:ri.conicet.gov.ar:11336/48106instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:29.926CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli
title Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli
spellingShingle Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli
Dalla Via, Maria Virginia
SYMBIOSIS
TRANSCRIPTOME
LIPOPOLYSACCHARIDES
EXOPOLYSACCHARIDE
NOD FACTOR
SIGNAL PERCEPTION AND INTEGRATION
title_short Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli
title_full Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli
title_fullStr Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli
title_full_unstemmed Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli
title_sort Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli
dc.creator.none.fl_str_mv Dalla Via, Maria Virginia
Narduzzi, Candela
Zanetti, María Eugenia
Aguilar, Orlando Mario
Blanco, Flavio Antonio
author Dalla Via, Maria Virginia
author_facet Dalla Via, Maria Virginia
Narduzzi, Candela
Zanetti, María Eugenia
Aguilar, Orlando Mario
Blanco, Flavio Antonio
author_role author
author2 Narduzzi, Candela
Zanetti, María Eugenia
Aguilar, Orlando Mario
Blanco, Flavio Antonio
author2_role author
author
author
author
dc.subject.none.fl_str_mv SYMBIOSIS
TRANSCRIPTOME
LIPOPOLYSACCHARIDES
EXOPOLYSACCHARIDE
NOD FACTOR
SIGNAL PERCEPTION AND INTEGRATION
topic SYMBIOSIS
TRANSCRIPTOME
LIPOPOLYSACCHARIDES
EXOPOLYSACCHARIDE
NOD FACTOR
SIGNAL PERCEPTION AND INTEGRATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Establishment of nitrogen fixing symbiosis requires the recognition of rhizobial molecules to initiate the development of nodules. Using transcriptional profiling of roots inoculated with mutant strains defective in the synthesis of Nod Factor (NF), exopolysaccharide (EPS) or lipopolysaccharide (LPS), we identified 2606 genes from common bean (Phaseolus vulgaris) that are differentially regulated at early stages of its interaction with Rhizobium etli. Many transcription factors from different families are modulated by NF, EPS and LPS in different combinations, suggesting that the plant response depends on the integration of multiple signals. Some receptors identified as differentially expressed constitute excellent candidates to participate in signal perception of molecules derived from the bacteria. Several components of the ethylene signal response, a hormone that plays a negative role during early stages of the process, were down-regulated by NF and LPS. In addition, genes encoding proteins involved in small RNA-mediated gene regulation were regulated by these signal molecules, such as Argonaute 7, a specific component of the TAS3 derived tasiRNAs biosynthetic pathway, an RNA dependent RNA polymerase and a XH/XP domain-containing protein, which is part of the RNA directed-DNA methylation. Interestingly, a number of genes encoding components of the circadian central oscillator were down-regulated by NF and LPS, suggesting that a root circadian clock is adjusted at early stages of symbiosis. Our results reveal a complex interaction of the responses triggered by NF, LPS and EPS that integrates information of the signals present in the surface or secreted by rhizobia.
Fil: Dalla Via, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Narduzzi, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Blanco, Flavio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Establishment of nitrogen fixing symbiosis requires the recognition of rhizobial molecules to initiate the development of nodules. Using transcriptional profiling of roots inoculated with mutant strains defective in the synthesis of Nod Factor (NF), exopolysaccharide (EPS) or lipopolysaccharide (LPS), we identified 2606 genes from common bean (Phaseolus vulgaris) that are differentially regulated at early stages of its interaction with Rhizobium etli. Many transcription factors from different families are modulated by NF, EPS and LPS in different combinations, suggesting that the plant response depends on the integration of multiple signals. Some receptors identified as differentially expressed constitute excellent candidates to participate in signal perception of molecules derived from the bacteria. Several components of the ethylene signal response, a hormone that plays a negative role during early stages of the process, were down-regulated by NF and LPS. In addition, genes encoding proteins involved in small RNA-mediated gene regulation were regulated by these signal molecules, such as Argonaute 7, a specific component of the TAS3 derived tasiRNAs biosynthetic pathway, an RNA dependent RNA polymerase and a XH/XP domain-containing protein, which is part of the RNA directed-DNA methylation. Interestingly, a number of genes encoding components of the circadian central oscillator were down-regulated by NF and LPS, suggesting that a root circadian clock is adjusted at early stages of symbiosis. Our results reveal a complex interaction of the responses triggered by NF, LPS and EPS that integrates information of the signals present in the surface or secreted by rhizobia.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48106
Dalla Via, Maria Virginia; Narduzzi, Candela; Zanetti, María Eugenia; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli; American Society of Plant Biologist; Plant Physiology; 169; 8-2015; 1356-1370
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48106
identifier_str_mv Dalla Via, Maria Virginia; Narduzzi, Candela; Zanetti, María Eugenia; Aguilar, Orlando Mario; Blanco, Flavio Antonio; Changes in the common bean (Phaseolus vulgaris) transcriptome in response to secreted and surface signal molecules of Rhizobium etli; American Society of Plant Biologist; Plant Physiology; 169; 8-2015; 1356-1370
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.15.00508
info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/169/2/1356
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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